FastQCFastQC Report
Fri 27 May 2016
SRR939772_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939772_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1800673
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80030.4444449380870375No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52190.2898360779552978No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44970.24973995833779927No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA101050.039.9008641
GGTATCA59450.035.2956161
TATCAAC129100.031.2332042
ATCAACG133400.030.4733263
TCAACGC135250.030.0225494
CAACGCA136400.029.7012625
AACGCAG141300.028.737886
GTACATG133950.028.484981
TACATGG134650.028.0239892
ACATGGG141600.025.9839653
ACGCAGA160500.025.1534987
CGCAGAG163950.024.3945948
CATGGGG45950.024.0633664
CATGGGA94950.023.339954
GCAGAGT163850.023.174659
GAGTACT68650.022.55004912-13
AGTACTT70950.022.31643312-13
ATGGGAT35900.022.1502345
ATGGGAG31950.020.7651145
GTACTTT76150.020.5505114-15
Produced by FastQC (version 0.11.5)