FastQCFastQC Report
Fri 27 May 2016
SRR939772_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939772_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1800673
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT89840.49892456875845864No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61480.3414278994575917No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53720.2983329010875378No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA104850.036.8863031
GGTATCA64150.035.2116361
TATCAAC138200.027.8391552
ATCAACG138050.027.759373
GTACATG140500.027.3917921
TCAACGC140200.027.2998454
TACATGG139950.027.1181662
CAACGCA142900.026.7840335
AACGCAG146650.026.0667956
ACATGGG144050.025.2861463
GTGTAAG4900.023.242941
CATGGGA95800.023.020894
ACGCAGA166450.022.823567
CGCAGAG169550.022.4622088
CATGGGG46450.022.1568834
AGTACTT75650.022.03692812-13
GTATAGA3251.8189894E-1221.9020021
ATGGGAT37500.021.6271615
ATGGGAG33250.021.5387235
CTACGCT1553.953992E-521.419094
Produced by FastQC (version 0.11.5)