##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939772_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1800673 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.365207341921604 31.0 31.0 34.0 30.0 34.0 2 31.58417658286652 31.0 31.0 34.0 30.0 34.0 3 31.630973530452227 31.0 31.0 34.0 30.0 34.0 4 35.447696500141895 37.0 35.0 37.0 33.0 37.0 5 35.18514522070359 37.0 35.0 37.0 32.0 37.0 6 35.21306978002114 37.0 35.0 37.0 32.0 37.0 7 35.107394846260256 37.0 35.0 37.0 32.0 37.0 8 35.068478840966684 37.0 35.0 37.0 32.0 37.0 9 36.63231136358461 39.0 35.0 39.0 32.0 39.0 10-11 36.500477599208736 38.0 35.0 39.0 32.0 39.0 12-13 36.40861055838567 38.0 35.0 39.0 32.0 39.0 14-15 37.394921787576095 39.0 36.0 41.0 32.0 41.0 16-17 37.33676269927966 39.0 36.0 41.0 32.0 41.0 18-19 37.336518346196115 39.0 36.0 41.0 32.0 41.0 20-21 37.268230544912925 39.0 36.0 40.0 31.0 41.0 22-23 37.130096080743144 39.0 36.0 40.0 31.0 41.0 24-25 36.95695248387686 39.0 36.0 40.0 31.0 41.0 26-27 36.64980065786514 38.5 35.0 40.0 30.0 41.0 28-29 36.39758606920857 38.0 35.0 40.0 30.0 41.0 30-31 36.09780259936146 38.0 35.0 40.0 29.5 41.0 32-33 36.042207274724504 38.0 35.0 40.0 30.0 41.0 34-35 35.86504740172147 38.0 34.0 40.0 29.0 41.0 36-37 35.86674843239167 38.0 35.0 40.0 29.0 41.0 38-39 35.746065776518 38.0 34.5 40.0 29.0 41.0 40-41 35.62023060266911 38.0 34.0 40.0 28.0 41.0 42-43 35.33939810282045 38.0 34.0 40.0 27.0 41.0 44-45 35.026075250753465 38.0 34.0 40.0 26.0 41.0 46-47 34.65782265852823 38.0 33.0 40.0 24.5 41.0 48-49 34.3075527872079 38.0 33.0 40.0 24.0 41.0 50-51 34.054628186239256 37.5 33.0 40.0 23.5 41.0 52-53 33.7696066970516 37.0 33.0 40.0 23.0 41.0 54-55 33.46685294886967 37.0 32.0 40.0 22.5 41.0 56-57 33.08630217701937 36.5 31.5 39.5 21.0 41.0 58-59 32.702324630846356 36.0 31.0 39.0 20.0 41.0 60-61 32.36646853704143 35.5 31.0 39.0 19.0 40.0 62-63 32.047039912299454 35.0 31.0 39.0 18.0 40.0 64-65 31.587431477008874 35.0 30.5 38.0 15.5 40.0 66-67 30.679903847061624 34.5 29.0 37.5 9.0 40.0 68-69 28.820807275946272 34.0 25.5 37.0 6.5 39.0 70-71 28.172756519368036 33.0 24.5 36.0 6.5 39.0 72-73 28.725370181037867 33.0 26.0 36.0 4.5 39.0 74-75 28.8047518899878 33.0 26.5 35.0 3.5 38.0 76-77 28.3215000724729 32.5 26.5 35.0 2.0 37.0 78-79 28.847928524501675 33.5 28.0 35.0 2.0 37.0 80-81 28.66479893906334 33.5 28.0 35.0 2.0 36.5 82-83 28.243894088488027 33.0 27.0 35.0 2.0 36.0 84-85 27.914085733500755 33.0 27.0 35.0 2.0 36.0 86-87 27.53990896736942 33.0 26.0 35.0 2.0 35.0 88-89 27.10708635049229 33.0 25.5 35.0 2.0 35.0 90-91 26.624851097339715 32.0 25.0 34.5 2.0 35.0 92-93 26.153823320502944 32.0 24.0 34.0 2.0 35.0 94-95 25.357259757879415 32.0 20.0 34.0 2.0 35.0 96-97 24.837058977393454 31.0 19.0 34.0 2.0 35.0 98-99 24.18098261039067 31.0 9.5 34.0 2.0 35.0 100-101 22.727589351314755 30.0 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.24874053515969763 1101 2 0.3026251782530167 1101 3 0.28411090621001733 1101 4 0.22492043075352797 1101 5 0.22445146641852887 1101 6 0.2902625524346476 1101 7 0.23074810012997915 1101 8 0.36055978537920197 1101 9 0.37687253771695595 1101 10-11 0.3318944115665019 1101 12-13 0.41460583563508635 1101 14-15 0.32539641985945167 1101 16-17 0.3728443078218149 1101 18-19 0.23427535222970874 1101 20-21 0.3911164441243713 1101 22-23 0.3893216861033082 1101 24-25 0.35913676699323105 1101 26-27 0.39671349669063005 1101 28-29 0.44077436386410085 1101 30-31 0.1291953273753137 1101 32-33 0.12699554351989661 1101 34-35 0.030446670367894058 1101 36-37 0.24533894461492878 1101 38-39 0.359046103375789 1101 40-41 0.47268999979720405 1101 42-43 0.25118724922190694 1101 44-45 0.3094955729190261 1101 46-47 0.3087956829612608 1101 48-49 0.30759925392543863 1101 50-51 0.19093673780223952 1101 52-53 0.06504919893730943 1101 54-55 0.03927237219226498 1101 56-57 -0.013586500269298085 1101 58-59 -0.0023436880390690362 1101 60-61 -0.23307402112236986 1101 62-63 -0.4024639481170311 1101 64-65 -0.5352008638397905 1101 66-67 -0.5403353626570677 1101 68-69 -8.970511580998132 1101 70-71 -7.024580388423203 1101 72-73 -2.2547724542941303 1101 74-75 -1.1772486435967124 1101 76-77 -0.9476952981174129 1101 78-79 -1.0594500113369243 1101 80-81 -1.032246519555862 1101 82-83 -0.9165516255229953 1101 84-85 -0.6686676752104503 1101 86-87 -0.79258763389754 1101 88-89 -0.7859855548761665 1101 90-91 -0.9453651173877802 1101 92-93 -1.057562917127317 1101 94-95 -0.7076491037222645 1101 96-97 -1.01774670179228 1101 98-99 -1.1665182613082017 1101 100-101 -1.2590045856537522 1102 1 0.22480522101546896 1102 2 0.21181428379309963 1102 3 0.32880759454180364 1102 4 0.23581334316455127 1102 5 0.40220757907714955 1102 6 0.24439440015981262 1102 7 0.36701241458371214 1102 8 0.15214598160328308 1102 9 0.40795269865783723 1102 10-11 0.30954916241763186 1102 12-13 0.3167591937285579 1102 14-15 0.5200157327656783 1102 16-17 0.4955725653272296 1102 18-19 0.3871544918149752 1102 20-21 0.26098567965517105 1102 22-23 0.35149825682444913 1102 24-25 0.39963583848812334 1102 26-27 0.24044376595711725 1102 28-29 0.22090125956201945 1102 30-31 0.06851287612183654 1102 32-33 0.053172423742736896 1102 34-35 -0.03567527330280029 1102 36-37 0.021713905927221333 1102 38-39 0.3376278118251861 1102 40-41 0.26411061261057966 1102 42-43 0.25343732659732865 1102 44-45 0.19158393565501797 1102 46-47 0.2838761534038454 1102 48-49 0.3883830669863997 1102 50-51 0.30849090060014106 1102 52-53 0.3106852249354546 1102 54-55 0.010028330100041671 1102 56-57 0.09700464798201125 1102 58-59 0.026491811806174326 1102 60-61 0.23620716117413565 1102 62-63 0.20733410202224434 1102 64-65 0.29650835931093766 1102 66-67 0.45141719073197706 1102 68-69 -4.722266455649944 1102 70-71 0.3833799953589079 1102 72-73 0.7945102755108842 1102 74-75 0.43956890514980884 1102 76-77 0.23539430262539085 1102 78-79 0.25802135975562024 1102 80-81 0.12792602764314864 1102 82-83 0.21975369790628108 1102 84-85 0.43179038730889374 1102 86-87 0.334440376006512 1102 88-89 0.1875435382838475 1102 90-91 0.0843749011823185 1102 92-93 -0.19423809509079604 1102 94-95 0.002559036172502971 1102 96-97 -0.35472287016120774 1102 98-99 -0.1318362743072754 1102 100-101 -0.30360532845783794 1103 1 0.22736872167756417 1103 2 0.1300964554555506 1103 3 0.23136507618379198 1103 4 0.19263108723581013 1103 5 0.1850160538574528 1103 6 0.18942870857440397 1103 7 0.05979079426349898 1103 8 0.17698587566852808 1103 9 0.19563296830993693 1103 10-11 0.32752887831988886 1103 12-13 0.3563369594682939 1103 14-15 0.30042923944678535 1103 16-17 0.329177356467234 1103 18-19 0.17916213597050756 1103 20-21 0.201787714083963 1103 22-23 0.24341802328647333 1103 24-25 0.3055955712436287 1103 26-27 0.20445333619907302 1103 28-29 0.13006461576762973 1103 30-31 0.24483465293656792 1103 32-33 0.1662737238510843 1103 34-35 -0.08607493623766516 1103 36-37 0.06177680468729818 1103 38-39 0.1195337345409655 1103 40-41 0.05745839159215649 1103 42-43 0.034725396899482064 1103 44-45 0.10188037239511516 1103 46-47 0.16983438140432838 1103 48-49 0.11149459942892292 1103 50-51 0.11574412077589358 1103 52-53 0.1945602700781066 1103 54-55 0.12517669894558736 1103 56-57 0.25215211397455306 1103 58-59 0.20340917066015862 1103 60-61 -0.018067245952387623 1103 62-63 0.005549161610545639 1103 64-65 -0.057583671506446166 1103 66-67 0.16842784439756286 1103 68-69 -4.418463638748534 1103 70-71 0.32842754561772836 1103 72-73 0.6142042083386201 1103 74-75 0.38969079030690423 1103 76-77 0.2528599942107981 1103 78-79 -0.05745955995065444 1103 80-81 -0.008699408735807879 1103 82-83 -0.0044900122177082835 1103 84-85 0.04802248072408588 1103 86-87 -0.06169150072395624 1103 88-89 -0.08815557090117565 1103 90-91 -0.22861173659305223 1103 92-93 -0.12649913999268492 1103 94-95 -0.3113211251372334 1103 96-97 -0.7966646060466047 1103 98-99 -0.42206469612020214 1103 100-101 -0.5887822876488826 1104 1 0.22298489004620237 1104 2 0.2537882790649668 1104 3 0.2956633865039784 1104 4 0.1724327285073386 1104 5 0.31292861926627324 1104 6 0.3011319888122941 1104 7 0.2289864098555796 1104 8 0.21988829838814894 1104 9 0.3774326040951905 1104 10-11 0.2804238669093664 1104 12-13 0.21774028119901345 1104 14-15 0.28329587460964234 1104 16-17 0.419910626792948 1104 18-19 0.11970177313884989 1104 20-21 0.2419135756362536 1104 22-23 0.2383126776282367 1104 24-25 0.3084833562186162 1104 26-27 0.49732319858122054 1104 28-29 0.3157121342665121 1104 30-31 0.43723628037715656 1104 32-33 0.32806604076401413 1104 34-35 0.564046948919426 1104 36-37 0.5326122510808844 1104 38-39 0.4428760387755375 1104 40-41 0.18703614452547157 1104 42-43 0.3098853171410738 1104 44-45 0.23550828553681669 1104 46-47 0.07260546782465127 1104 48-49 0.28365493459870095 1104 50-51 0.2511268344062856 1104 52-53 -0.013960165135465274 1104 54-55 -0.1747529937770338 1104 56-57 -0.19066674681703688 1104 58-59 -0.06837225438767547 1104 60-61 -0.05275264970056526 1104 62-63 -0.016921217126693477 1104 64-65 0.005651385315662338 1104 66-67 0.16345619782417486 1104 68-69 -4.005989859905263 1104 70-71 0.5634981986686007 1104 72-73 0.6862124031704582 1104 74-75 0.3730795434476768 1104 76-77 0.3376992671643997 1104 78-79 0.011094645807627757 1104 80-81 -0.05789548535921796 1104 82-83 -0.21844370162090243 1104 84-85 -0.1798289980338943 1104 86-87 0.03962359113653591 1104 88-89 -0.1822673364206473 1104 90-91 -0.4057137512999489 1104 92-93 -0.46115889887330397 1104 94-95 -0.15745278415846542 1104 96-97 -0.5975183784354385 1104 98-99 -0.4629178345909608 1104 100-101 -0.5046336972521672 1105 1 0.25477876598901616 1105 2 0.18424814622695962 1105 3 0.0918499225841316 1105 4 0.17229482464603052 1105 5 0.16056736743693278 1105 6 0.2322250879904999 1105 7 0.12188013945143439 1105 8 0.2622782567355557 1105 9 0.24139185209698866 1105 10-11 0.18257826294674118 1105 12-13 0.19695760642697735 1105 14-15 0.2523702301201709 1105 16-17 0.15607521082987574 1105 18-19 0.1694428516033355 1105 20-21 0.2890147801842673 1105 22-23 0.25992418275037465 1105 24-25 0.3091728755251637 1105 26-27 0.3475469405602851 1105 28-29 0.27508115374191533 1105 30-31 0.1623488549578127 1105 32-33 0.174481947552259 1105 34-35 0.2355416579141334 1105 36-37 0.08318215989547184 1105 38-39 0.3280907747881443 1105 40-41 0.31744394594391423 1105 42-43 0.2675643107243175 1105 44-45 0.03987758644866801 1105 46-47 -0.05985400532630791 1105 48-49 0.002734918838257272 1105 50-51 0.00911259372183082 1105 52-53 -0.04301847876825349 1105 54-55 -0.02800077042906679 1105 56-57 -0.07325990228253687 1105 58-59 0.04986482767919398 1105 60-61 0.11291351038048703 1105 62-63 0.1438817210698602 1105 64-65 0.1504898407924209 1105 66-67 0.7384674552822403 1105 68-69 1.737945184561699 1105 70-71 2.0099408419197538 1105 72-73 1.2243118628124705 1105 74-75 0.7408255189064192 1105 76-77 0.5563599110909969 1105 78-79 0.5738943756286332 1105 80-81 0.4325159747330929 1105 82-83 0.4676505233031101 1105 84-85 0.3951766306951363 1105 86-87 0.2568081008742382 1105 88-89 0.5138530620933714 1105 90-91 0.45363416044157745 1105 92-93 0.5507486773959762 1105 94-95 0.7910907822042503 1105 96-97 0.4269173414790046 1105 98-99 0.6980975881265898 1105 100-101 0.6315534017008879 1106 1 0.5011969540454295 1106 2 0.3862435594851732 1106 3 0.4141678524105892 1106 4 0.3370401160124814 1106 5 0.45544610770816973 1106 6 0.45122033872636536 1106 7 0.36144169027578243 1106 8 0.49302387314670426 1106 9 0.6063448109066059 1106 10-11 0.5416433982917326 1106 12-13 0.5588136116276843 1106 14-15 0.6625811480102684 1106 16-17 0.655585837985555 1106 18-19 0.7535301080455454 1106 20-21 0.6772039200798972 1106 22-23 0.5537745177269997 1106 24-25 0.7504066007179304 1106 26-27 0.7551394428626921 1106 28-29 0.7098925375294058 1106 30-31 0.7814082117304864 1106 32-33 0.7973732201022372 1106 34-35 0.851802921615672 1106 36-37 0.9034080659575565 1106 38-39 1.0448206445896915 1106 40-41 0.9882061757505127 1106 42-43 0.8779386538631613 1106 44-45 1.0449239053175106 1106 46-47 1.0920312538968275 1106 48-49 1.2193510229970244 1106 50-51 1.1371133882755302 1106 52-53 1.2929946674838035 1106 54-55 1.3310986751891534 1106 56-57 1.2103759031962724 1106 58-59 1.3262178262164852 1106 60-61 1.3049661676208544 1106 62-63 1.3116372874602433 1106 64-65 1.3021900051205684 1106 66-67 1.7243153704816905 1106 68-69 3.2247528845093285 1106 70-71 3.505264712840898 1106 72-73 2.382857829549561 1106 74-75 2.0138152077664166 1106 76-77 1.7080763276538313 1106 78-79 1.46755871280833 1106 80-81 1.4968215528611992 1106 82-83 1.3361293141368478 1106 84-85 1.419898085450722 1106 86-87 1.5617848962947178 1106 88-89 1.5784584036611697 1106 90-91 1.3698584051641163 1106 92-93 1.4426153368394274 1106 94-95 1.7807624870636687 1106 96-97 1.7314128901725034 1106 98-99 1.9085875027120665 1106 100-101 1.799801952543298 1107 1 0.17384949971078711 1107 2 0.16083636394463952 1107 3 0.19058199814092447 1107 4 0.22884092855494487 1107 5 0.15975271611615938 1107 6 0.0973867638607544 1107 7 0.08728649260419274 1107 8 0.16973640367159248 1107 9 0.2138924370110047 1107 10-11 0.2310783461031818 1107 12-13 0.05944714139918972 1107 14-15 0.091279273877376 1107 16-17 0.23253432463582158 1107 18-19 0.1443937329207614 1107 20-21 0.29598582764545966 1107 22-23 0.20080403436544714 1107 24-25 0.16160484932100871 1107 26-27 0.1307665327836105 1107 28-29 0.18954685241113367 1107 30-31 0.2636407397339795 1107 32-33 0.19601072954987586 1107 34-35 0.17316389084803774 1107 36-37 -0.008582814607912326 1107 38-39 0.048674251178759675 1107 40-41 -0.04210142787956528 1107 42-43 0.054395711727273977 1107 44-45 0.1269960886177799 1107 46-47 0.11320457345949109 1107 48-49 0.19589875395733714 1107 50-51 0.3891247430200764 1107 52-53 0.27819543626559096 1107 54-55 0.018875929697500737 1107 56-57 0.09901376418399366 1107 58-59 0.386054796474383 1107 60-61 0.4182208650751633 1107 62-63 0.3880733135299721 1107 64-65 0.2437316174652011 1107 66-67 0.6153041557470722 1107 68-69 1.9601203317768032 1107 70-71 2.4836724241755803 1107 72-73 1.5536591833425284 1107 74-75 0.9178787080267377 1107 76-77 0.5793185611940288 1107 78-79 0.6563490694578604 1107 80-81 0.6870894385233939 1107 82-83 0.7472380109353516 1107 84-85 0.5408272664896216 1107 86-87 0.661790017870004 1107 88-89 0.7451699219930781 1107 90-91 0.8421284085522345 1107 92-93 0.7373485088279885 1107 94-95 0.9443190847133209 1107 96-97 0.41190887079658367 1107 98-99 0.775310324875818 1107 100-101 0.7325170299154529 1108 1 -0.017218246417478156 1108 2 -0.13563303421456396 1108 3 -0.12723646676088052 1108 4 -0.08563541162472177 1108 5 -0.019554873030131148 1108 6 -0.022380025127546332 1108 7 -0.11086593790670918 1108 8 0.025340426047726794 1108 9 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-0.5057061405356365 2316 64-65 -0.4371352153552408 2316 66-67 0.10976798088265838 2316 68-69 1.4386376237326388 2316 70-71 1.580683682728747 2316 72-73 0.537561543427092 2316 74-75 -0.029105295511715923 2316 76-77 -0.2012052931114674 2316 78-79 -0.39539037612579975 2316 80-81 -0.5766896161152744 2316 82-83 -0.421387120420313 2316 84-85 -0.48176957838999 2316 86-87 -0.5399020838734323 2316 88-89 -0.4608813667755669 2316 90-91 -0.41404112233844614 2316 92-93 -0.36183977339534223 2316 94-95 -0.009951376665913614 2316 96-97 -0.011912628828365968 2316 98-99 -0.2882245506762544 2316 100-101 -0.4829125684431368 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 16.0 4 46.0 5 113.0 6 277.0 7 653.0 8 1470.0 9 2431.0 10 3997.0 11 5779.0 12 8039.0 13 10039.0 14 12482.0 15 15487.0 16 18916.0 17 22062.0 18 19025.0 19 15556.0 20 15563.0 21 16005.0 22 17389.0 23 19390.0 24 22944.0 25 27544.0 26 33647.0 27 41400.0 28 50319.0 29 60639.0 30 71905.0 31 85574.0 32 101718.0 33 123635.0 34 153405.0 35 193748.0 36 237842.0 37 249457.0 38 133839.0 39 8322.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.797513917418684 19.466222138777578 14.077062116514153 22.659201827289582 2 16.48699707651553 21.534076674948853 37.91519950851243 24.063726740023185 3 19.692192863446056 26.09518774369361 29.208134958429433 25.004484434430907 4 13.367446504723512 16.669489685245463 35.60196659804418 34.36109721198685 5 13.8394922342924 38.768504886783994 32.2728224391658 15.119180439757802 6 32.28481795417602 35.396765542660994 17.1826311606827 15.135785342480284 7 29.363965583978878 33.223633608100975 19.559964524375054 17.8524362835451 8 27.979483226549185 33.314488527345056 19.419739175297234 19.286289070808525 9 28.231111367805262 15.906386112303567 19.145563908605283 36.71693861128589 10-11 25.96309824160189 26.59869393276847 26.03287770739052 21.405330118239124 12-13 27.049636441486047 23.971648378134176 26.862650797785047 22.11606438259473 14-15 23.671482828920077 24.886445234642824 25.6020387932734 25.840033143163694 16-17 22.884393885373523 28.490541832855175 25.792234650956924 22.832829630814377 18-19 22.778649982534308 26.33384851108447 29.00626599054909 21.881235515832138 20-21 23.877822347533396 25.506435649337778 28.574233078410128 22.041508924718702 22-23 23.412996140887323 25.69497626720676 28.52694520326567 22.365082388640246 24-25 22.810629256918876 25.968951027436155 28.861455295982637 22.358964419662332 26-27 22.733153122383783 26.273534675335675 28.617970835815054 22.37534136646549 28-29 22.551982011150287 26.170855197867905 28.887453621455556 22.38970916952625 30-31 22.48849106066618 26.28903036127476 28.754674313469103 22.46780426458995 32-33 22.16453264920374 26.29518999840889 28.508438835554266 23.031838516833105 34-35 22.805803521221996 26.09846237066439 28.575169395253187 22.520564712860434 36-37 22.961494297762716 25.843675115485976 28.383490988355366 22.81133959839594 38-39 22.645845852300553 25.936678160491443 28.633826532001954 22.783649455206046 40-41 22.57327728065182 26.005957357859533 28.850740277876607 22.57002508361204 42-43 22.705837452616546 25.867217640196237 28.374645692535754 23.052299214651466 44-45 22.409357388327614 26.099463109782878 28.375409560848485 23.115769941041027 46-47 22.55979557344467 26.24362591430105 27.834413156096204 23.362165356158073 48-49 22.575289040877347 26.313684464473226 27.61348596166578 23.497540532983653 50-51 22.433526442117948 26.572886059146654 27.50352142818232 23.490066070553077 52-53 22.80949800619677 26.33440317896989 27.427563113945507 23.428535700887835 54-55 22.06970459623781 26.14505842336112 27.837299260865432 23.947937719535638 56-57 22.380838495005545 26.595618502077585 27.29765592166647 23.725887081250402 58-59 22.325247880418647 26.056555309902247 27.358200014496663 24.259996795182442 60-61 22.39266376263755 26.162616003336876 27.584897863744644 23.85982237028093 62-63 22.42090791683061 26.186655455828582 27.32686211030143 24.065574517039373 64-65 22.11057257884018 26.100580204190553 27.365011027045277 24.423836189923986 66-67 21.925749801860427 26.393498241076767 27.441010025167202 24.239741931895605 68-69 21.83439134540825 26.041540322821245 27.74085633035294 24.38321200141756 70-71 21.57310418365427 26.210820492448793 27.544098071602306 24.67197725229463 72-73 21.84492877832865 26.057754548592825 27.13975870837127 24.957557964707252 74-75 22.025716149088336 26.175981076754027 26.672060769484602 25.12624200467304 76-77 21.722668967254673 26.018439331388834 27.15128078996978 25.10761091138672 78-79 21.771306039550566 25.99452621126181 26.906231932914988 25.327935816272635 80-81 21.5399122982393 26.338129384532678 26.918198337487393 25.203759979740624 82-83 21.804958479412974 26.12892512965985 26.485458492463653 25.58065789846352 84-85 21.490340360839415 25.729780011523534 26.502995425294856 26.276884202342192 86-87 21.63210133240922 25.567351309084284 26.77711024031349 26.023437118193005 88-89 21.48495323544937 25.401718638539723 26.71711827184278 26.396209854168127 90-91 21.36946224807196 25.542341904065335 26.82026009308793 26.26793575477477 92-93 21.545449723887003 25.30334665003167 26.777670931953217 26.37353269412811 94-95 21.08635476017322 25.041149169944195 27.030426347918418 26.84206972196417 96-97 21.33237389052854 24.75215242809664 26.91606439968217 26.99940928169265 98-99 21.007630857146665 24.752738676747825 26.843086958888467 27.396543507217043 100-101 20.981604189744857 24.617805749152385 27.099822465143546 27.300767595959215 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 744.0 1 528.5 2 446.5 3 625.5 4 575.0 5 580.0 6 961.0 7 1955.5 8 2833.0 9 2904.0 10 2485.5 11 2090.0 12 2041.5 13 2083.5 14 2142.5 15 2188.0 16 2217.5 17 2346.5 18 2547.0 19 2766.5 20 3107.5 21 3436.5 22 3644.0 23 4078.5 24 4653.5 25 5361.0 26 6406.0 27 7657.5 28 8940.0 29 10378.0 30 12318.5 31 14736.5 32 18366.5 33 21739.0 34 24525.5 35 27988.0 36 32378.0 37 37358.5 38 41028.0 39 43236.0 40 45549.0 41 49874.5 42 54462.0 43 58492.0 44 62859.5 45 66304.5 46 68352.0 47 70558.5 48 76727.0 49 78869.5 50 76301.5 51 74298.5 52 71476.0 53 70752.5 54 69227.0 55 64908.0 56 60050.0 57 54939.5 58 49719.5 59 44103.5 60 38104.0 61 31305.0 62 24340.0 63 18224.0 64 13546.5 65 10761.0 66 8372.0 67 6319.0 68 4339.0 69 2730.0 70 1962.0 71 1617.5 72 1352.0 73 1152.5 74 902.0 75 649.0 76 427.0 77 261.0 78 157.0 79 93.0 80 66.5 81 51.5 82 32.0 83 19.0 84 12.5 85 8.0 86 7.5 87 6.5 88 3.0 89 0.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.5 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02282479939444863 2 0.023546751686730462 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 3.8874354199790856E-4 18-19 0.0 20-21 0.0 22-23 0.0 24-25 8.33021875709804E-5 26-27 3.054413544269281E-4 28-29 1.1106958342797386E-4 30-31 4.1651093785490206E-4 32-33 0.002193624272702484 34-35 0.00424841156612 36-37 0.10337801477558668 38-39 0.09640839841548132 40-41 0.10490522154772133 42-43 0.10648796311156995 44-45 0.09968495112660655 46-47 0.09382603060078093 48-49 0.09474235466406172 50-51 0.09368719362149597 52-53 0.07486089923045439 54-55 0.06308752338708916 56-57 0.03479254700881282 58-59 0.014355743658065622 60-61 0.01032947125880157 62-63 0.0019159503141325492 64-65 0.005553479171398694 66-67 0.14983286804433676 68-69 0.4138175004567737 70-71 0.39349176668945446 72-73 0.17890533150660892 74-75 0.031043948568118696 76-77 0.03073850721369177 78-79 0.005109200837686799 80-81 0.0013883697928496735 82-83 0.0 84-85 5.831153129968628E-4 86-87 0.0013883697928496735 88-89 1.9437177099895428E-4 90-91 5.553479171398693E-4 92-93 0.0028600417732703273 94-95 0.07922038038000236 96-97 0.017909970327760788 98-99 0.019492711891609418 100-101 0.010079564696088629 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1800673.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 44.50362700153737 #Duplication Level Percentage of deduplicated Percentage of total 1 70.89296101072739 31.549938938559436 2 13.965685523214196 12.430473186917895 3 5.797765208532718 7.740647408490918 4 2.895397299271616 5.154227257121706 5 1.7145468932942716 3.8151777707906476 6 1.074005723552653 2.8678290071102115 7 0.7098768238863309 2.211446537109131 8 0.490744873249542 1.7471941433611482 9 0.3474501076761881 1.3916510994298565 >10 1.8593563895063392 14.90150638092321 >50 0.1493851563661741 4.638351976131946 >100 0.09544498771301148 7.981948183319769 >500 0.0057539006516006625 1.723514389564607 >1k 0.0012508479677392744 0.7046919885500584 >5k 3.752543903217823E-4 1.1414017326195118 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8984 0.49892456875845864 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6148 0.3414278994575917 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5372 0.2983329010875378 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 2.776739585699347E-5 0.0 14-15 0.0 0.0 0.0 1.9437177099895428E-4 0.0 16-17 0.0 0.0 0.0 3.6097614614091506E-4 0.0 18-19 0.0 0.0 0.0 4.7204572956888896E-4 0.0 20-21 0.0 0.0 0.0 8.33021875709804E-4 0.0 22-23 0.0 0.0 0.0 0.0021658568768454906 0.0 24-25 0.0 0.0 0.0 0.004331713753690981 0.0 26-27 0.0 0.0 0.0 0.008774497090809935 0.0 28-29 0.0 0.0 0.0 0.019492711891609414 0.0 30-31 0.0 0.0 0.0 0.054174189316994255 0.0 32-33 0.0 0.0 0.0 0.12861857760959375 0.0 34-35 0.0 0.0 0.0 0.24432531614568553 0.0 36-37 0.0 0.0 0.0 0.43578151057965553 0.0 38-39 0.0 0.0 0.0 0.7426945369870044 0.0 40-41 0.0 0.0 0.0 1.0905089374917045 0.0 42-43 0.0 0.0 0.0 1.4274662862163203 0.0 44-45 0.0 0.0 0.0 1.8175148958195075 0.0 46-47 0.0 0.0 0.0 2.2686240089122234 0.0 48-49 0.0 0.0 0.0 2.7421414104615334 0.0 50-51 0.0 0.0 0.0 3.2631688263221585 0.0 52-53 0.0 0.0 0.0 3.755345917887368 0.0 54-55 0.0 0.0 0.0 4.263628099049633 0.0 56-57 0.0 0.0 0.0 4.808702079722415 0.0 58-59 0.0 0.0 0.0 5.371047380618247 2.776739585699347E-5 60-61 0.0 0.0 0.0 5.973405498944006 5.553479171398694E-5 62-63 0.0 0.0 0.0 6.544691901305789 5.553479171398694E-5 64-65 0.0 0.0 0.0 7.111035707205028 5.553479171398694E-5 66-67 0.0 0.0 0.0 7.698371664372154 5.553479171398694E-5 68-69 0.0 0.0 0.0 8.344407896380964 5.553479171398694E-5 70-71 0.0 0.0 0.0 9.09826492650248 5.553479171398694E-5 72-73 0.0 0.0 0.0 9.859313712150957 5.553479171398694E-5 74-75 0.0 0.0 0.0 10.616752736338025 5.553479171398694E-5 76-77 0.0 0.0 0.0 11.407318263782486 5.553479171398694E-5 78-79 0.0 0.0 0.0 12.250086495438094 5.553479171398694E-5 80-81 0.0 0.0 0.0 13.132256661814777 5.553479171398694E-5 82-83 0.0 0.0 0.0 14.02947675674595 5.553479171398694E-5 84-85 0.0 0.0 0.0 14.89182100248074 5.553479171398694E-5 86-87 0.0 0.0 0.0 15.751360741233972 5.553479171398694E-5 88-89 0.0 0.0 0.0 16.64366600709846 5.553479171398694E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 10485 0.0 36.886303 1 GGTATCA 6415 0.0 35.211636 1 TATCAAC 13820 0.0 27.839155 2 ATCAACG 13805 0.0 27.75937 3 GTACATG 14050 0.0 27.391792 1 TCAACGC 14020 0.0 27.299845 4 TACATGG 13995 0.0 27.118166 2 CAACGCA 14290 0.0 26.784033 5 AACGCAG 14665 0.0 26.066795 6 ACATGGG 14405 0.0 25.286146 3 GTGTAAG 490 0.0 23.24294 1 CATGGGA 9580 0.0 23.02089 4 ACGCAGA 16645 0.0 22.82356 7 CGCAGAG 16955 0.0 22.462208 8 CATGGGG 4645 0.0 22.156883 4 AGTACTT 7565 0.0 22.036928 12-13 GTATAGA 325 1.8189894E-12 21.902002 1 ATGGGAT 3750 0.0 21.627161 5 ATGGGAG 3325 0.0 21.538723 5 CTACGCT 155 3.953992E-5 21.41909 4 >>END_MODULE