FastQCFastQC Report
Fri 27 May 2016
SRR939771_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939771_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1105141
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92670.838535535284638No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70350.636570356180795No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48260.43668635947811185No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA26690.2415076447258766No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA18440.16685653685819274No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14980.13554831464944292No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA14480.1310240050817045No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14360.12993817078544728No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN11960.10822148486030289No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA92600.034.070271
GGTATCA58450.033.603211
TACATGG88400.030.2148952
GTACATG88050.029.7423061
ACATGGG92250.028.0821653
TATCAAC114850.027.8002682
ATCAACG116600.027.5482773
TCAACGC117850.027.3768624
CAACGCA118900.026.975485
AACGCAG122450.026.2309826
CATGGGA66200.025.4433734
ATGGGAT24800.023.3406245
GAGTACT64450.023.18742812-13
ACGCAGA136900.023.185017
CGCAGAG137800.023.0335858
CATGGGG23300.022.3996414
CTGGTCG3059.094947E-1221.7777429
AGTACTT65900.021.74134612-13
GCAGAGT136850.021.6680499
TATTCGC550.00288717721.57931722-23
Produced by FastQC (version 0.11.5)