FastQCFastQC Report
Fri 27 May 2016
SRR939771_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939771_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1105141
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT98060.887307592424858No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82500.7465110786768385No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61720.55848077304163No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA31460.2846695580021011No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19520.17662904552450773No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA18870.1707474430864478No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA17250.15608868008697535No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16940.15328360815497752No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT11060.10007772763837375No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA61650.036.03611
GTATCAA94050.032.7494771
GTACATG89850.026.7208141
TACATGG88650.026.7088572
ATCAACG116650.026.3605143
TCAACGC118050.026.0478944
TATCAAC118250.025.9264162
ACATGGG88300.025.7974873
CAACGCA120600.025.5362365
AACGCAG122500.025.045026
CATGGGA63000.023.5809354
ATGGGGG11600.023.5809335
CATGGGG22600.023.1635724
GAGTACT69850.022.73787312-13
CGCAGAG135400.022.5892878
ACGCAGA136350.022.4664617
AGTACTT71700.021.88826812-13
GTAAGGT4150.021.592184
GCAGAGT140400.021.5162939
ATAACGC1100.002999057521.4372123
Produced by FastQC (version 0.11.5)