##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939771_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1105141 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.41464663784983 31.0 31.0 34.0 30.0 34.0 2 31.608601074433036 31.0 31.0 34.0 30.0 34.0 3 31.646277714789335 31.0 31.0 34.0 30.0 34.0 4 35.46615318769279 37.0 35.0 37.0 33.0 37.0 5 35.21845447775442 37.0 35.0 37.0 33.0 37.0 6 35.263426114857744 37.0 35.0 37.0 32.0 37.0 7 35.18487143269501 37.0 35.0 37.0 32.0 37.0 8 35.1304494177666 37.0 35.0 37.0 32.0 37.0 9 36.70394365967781 39.0 35.0 39.0 32.0 39.0 10-11 36.563927136899274 38.5 35.0 39.0 32.0 39.0 12-13 36.499987784364166 38.0 35.0 39.0 32.0 39.0 14-15 37.451692589452385 39.0 36.0 41.0 32.0 41.0 16-17 37.40548445854421 39.0 36.0 41.0 32.0 41.0 18-19 37.46540350959742 39.0 36.0 41.0 32.0 41.0 20-21 37.43058532802601 39.0 36.0 41.0 32.0 41.0 22-23 37.28440714804717 39.0 36.0 40.5 31.0 41.0 24-25 37.10902183522283 39.0 36.0 40.0 31.0 41.0 26-27 36.799943174671824 39.0 35.5 40.0 30.5 41.0 28-29 36.55124775933569 38.5 35.0 40.0 30.0 41.0 30-31 36.25134801803571 38.0 35.0 40.0 30.0 41.0 32-33 36.11455370853131 38.0 35.0 40.0 30.0 41.0 34-35 36.091862033894316 38.0 35.0 40.0 30.0 41.0 36-37 35.997910673841616 38.0 35.0 40.0 30.0 41.0 38-39 35.8316468215368 38.0 35.0 40.0 29.0 41.0 40-41 35.602806338738674 38.0 34.5 40.0 28.5 41.0 42-43 35.337470060381435 38.0 34.0 40.0 27.0 41.0 44-45 34.998515574030826 38.0 34.0 40.0 26.0 41.0 46-47 34.56310461741986 38.0 33.0 40.0 24.5 41.0 48-49 34.15435225007488 38.0 33.0 40.0 23.5 41.0 50-51 33.860048174848274 38.0 33.0 40.0 22.5 41.0 52-53 33.55320271349991 37.0 33.0 40.0 21.5 41.0 54-55 33.24152619439511 37.0 32.5 40.0 19.5 41.0 56-57 31.981498288453693 36.5 30.5 39.5 12.5 41.0 58-59 28.74423851798096 34.0 23.0 38.5 2.0 40.0 60-61 28.720667317564004 34.0 23.0 38.0 2.0 40.0 62-63 30.545078863240075 35.0 28.5 38.0 2.0 40.0 64-65 30.09153085443396 35.0 28.0 38.0 2.0 40.0 66-67 24.750472111703395 28.0 16.5 36.0 2.0 39.0 68-69 17.197032324382135 13.0 7.0 29.0 2.0 35.5 70-71 16.109628997566826 12.5 7.5 25.5 2.0 33.5 72-73 18.18776065678497 17.0 7.0 30.0 2.0 34.5 74-75 18.88649683615032 17.5 7.5 30.5 2.0 35.0 76-77 19.844354249819705 22.5 7.5 31.0 2.0 34.5 78-79 22.87389663400417 27.5 10.5 34.0 2.0 36.0 80-81 25.441514250217846 31.5 20.0 34.5 2.0 36.0 82-83 26.362318925820325 32.5 24.5 35.0 2.0 36.0 84-85 26.334644176625424 33.0 24.5 35.0 2.0 36.0 86-87 26.034088410438123 33.0 23.5 35.0 2.0 35.5 88-89 25.58356942688761 33.0 21.0 35.0 2.0 35.0 90-91 24.00917168035572 31.5 9.5 34.5 2.0 35.0 92-93 22.006157132890735 30.5 2.0 34.0 2.0 35.0 94-95 17.677084643497977 20.0 2.0 33.0 2.0 35.0 96-97 16.406677971408172 13.5 2.0 32.5 2.0 34.5 98-99 15.331448656777733 2.0 2.0 32.0 2.0 34.0 100-101 14.72643852684861 2.0 2.0 31.5 2.0 34.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.4631499610948566 1101 2 0.4711155084040435 1101 3 0.43735443961009324 1101 4 0.2954679842185328 1101 5 0.41690245921882507 1101 6 0.34833776848590503 1101 7 0.49545400869058653 1101 8 0.34735033950230587 1101 9 0.5035709925213894 1101 10-11 0.6698899902571966 1101 12-13 0.6873338049783655 1101 14-15 0.5007617604783761 1101 16-17 0.548206040579835 1101 18-19 0.8479734464618573 1101 20-21 0.6739213371908264 1101 22-23 0.681294744334636 1101 24-25 0.7799069781244157 1101 26-27 0.5491793618394283 1101 28-29 0.59474600207799 1101 30-31 0.48084705005599915 1101 32-33 0.47456252045683556 1101 34-35 0.2265367857035514 1101 36-37 0.35851738986048076 1101 38-39 0.18637204875192737 1101 40-41 -0.1535392832882394 1101 42-43 -0.05304941609455227 1101 44-45 -0.0468831644678076 1101 46-47 -0.03829420643283754 1101 48-49 -0.41750857581948964 1101 50-51 -0.41153642273778956 1101 52-53 -0.07733943923975772 1101 54-55 -0.2943406716761672 1101 56-57 0.5360974701294836 1101 58-59 -8.434851017710454 1101 60-61 -9.653523776034994 1101 62-63 -2.975287514236861 1101 64-65 -0.6532603521279761 1101 66-67 -1.0544354320591651 1101 68-69 -3.979412249337024 1101 70-71 -4.615970594640045 1101 72-73 -6.832461754269346 1101 74-75 -7.723283484269139 1101 76-77 -8.60313830703732 1101 78-79 -11.769065229846063 1101 80-81 -8.326281241936478 1101 82-83 -4.4653375585676365 1101 84-85 -3.394076576449674 1101 86-87 -3.3592359859544665 1101 88-89 -3.021417168475068 1101 90-91 -7.930496942764918 1101 92-93 -13.79023906405363 1101 94-95 -11.890841862415744 1101 96-97 -11.485960467367178 1101 98-99 -11.217119639369194 1101 100-101 -11.501995178702426 1102 1 0.48842140344104834 1102 2 0.5276475377717027 1102 3 0.4758716524782223 1102 4 0.3800741292515468 1102 5 0.3330023130438917 1102 6 0.4426146764004315 1102 7 0.5235518155927963 1102 8 0.5423410643830806 1102 9 0.6550977742800868 1102 10-11 0.763979737400625 1102 12-13 0.6813565059215136 1102 14-15 0.7113223662159243 1102 16-17 0.8512726342774926 1102 18-19 0.6366061541096215 1102 20-21 0.8363567489563692 1102 22-23 0.6313891539134815 1102 24-25 0.8088422702492082 1102 26-27 0.9213260485014914 1102 28-29 1.0072068819231461 1102 30-31 0.823230435865419 1102 32-33 0.5552039370032773 1102 34-35 0.6488200523460463 1102 36-37 0.7168047029662432 1102 38-39 0.6265374609676044 1102 40-41 0.9275309446451558 1102 42-43 0.9140173955624817 1102 44-45 0.9149040550575691 1102 46-47 0.6888585570314874 1102 48-49 0.572650084393679 1102 50-51 0.704826640728669 1102 52-53 0.6797389100496218 1102 54-55 0.41744121732091344 1102 56-57 1.1034338880618577 1102 58-59 -8.410696617181667 1102 60-61 -9.373364239246055 1102 62-63 -2.5983125535050853 1102 64-65 0.0710648622184884 1102 66-67 -1.4218438713515589 1102 68-69 -3.5237468454132816 1102 70-71 -3.8977577194004667 1102 72-73 -6.409176348308158 1102 74-75 -7.358303396753708 1102 76-77 -8.328935931753914 1102 78-79 -11.296119437765096 1102 80-81 -7.264674549635892 1102 82-83 -3.0706889351469577 1102 84-85 -2.1831511256646685 1102 86-87 -1.8560791286444136 1102 88-89 -1.9155765678548349 1102 90-91 -7.605438958462731 1102 92-93 -13.391260867098902 1102 94-95 -11.594806827814693 1102 96-97 -11.391657499092116 1102 98-99 -11.05382660482974 1102 100-101 -10.84520183499233 1103 1 0.34380182646387425 1103 2 0.33522996501394076 1103 3 0.30129781957764834 1103 4 0.1680919197416486 1103 5 0.185302702527828 1103 6 0.1575619675937716 1103 7 0.33985029822829915 1103 8 0.27476948994837613 1103 9 0.37346403793907257 1103 10-11 0.376129218764909 1103 12-13 0.3327616233076469 1103 14-15 0.6229928533413087 1103 16-17 0.3597524467031121 1103 18-19 0.6027287435015936 1103 20-21 0.43740154298969003 1103 22-23 0.47340177495102154 1103 24-25 0.5338829260157354 1103 26-27 0.22021481905759543 1103 28-29 0.3263140779093021 1103 30-31 0.31868856972347714 1103 32-33 0.3857040451932576 1103 34-35 0.41269668533858095 1103 36-37 0.023181826194630162 1103 38-39 0.01543480219880422 1103 40-41 0.023959069307061043 1103 42-43 -0.008540976313540227 1103 44-45 -0.04350915291062307 1103 46-47 -0.06623355659504426 1103 48-49 -0.2606883203450465 1103 50-51 -0.07708475505733503 1103 52-53 -0.09152929629572526 1103 54-55 -0.08991358834283858 1103 56-57 0.6120776759283473 1103 58-59 -6.40128831495295 1103 60-61 -8.523521368289657 1103 62-63 -2.6299471350803323 1103 64-65 -0.376886036434545 1103 66-67 -1.847587931045382 1103 68-69 -4.0042595475933105 1103 70-71 -4.363844808954724 1103 72-73 -7.247230737440475 1103 74-75 -7.528233935404581 1103 76-77 -8.49016436137633 1103 78-79 -11.428888830819298 1103 80-81 -7.773111255115715 1103 82-83 -4.101387842428142 1103 84-85 -3.153181655525099 1103 86-87 -2.8455910396851998 1103 88-89 -3.0460490368854494 1103 90-91 -8.837877949455915 1103 92-93 -14.513677831288017 1103 94-95 -12.398191821864245 1103 96-97 -12.14649688768348 1103 98-99 -11.749918833408124 1103 100-101 -11.529047464793262 1104 1 0.15214203548477911 1104 2 0.12660441495893338 1104 3 0.20171207975366556 1104 4 0.19596423822349607 1104 5 0.08196111836941355 1104 6 0.02370092523953815 1104 7 0.22232760845932376 1104 8 0.268744668716252 1104 9 0.21558106214148864 1104 10-11 0.2804239451125383 1104 12-13 0.3504321403593522 1104 14-15 0.25061420922690303 1104 16-17 0.15544568790222968 1104 18-19 0.28310810956319443 1104 20-21 0.2704469569060848 1104 22-23 0.14076030767929382 1104 24-25 0.2647212442225566 1104 26-27 0.38265723517420014 1104 28-29 0.12369615986749949 1104 30-31 0.3280953697784881 1104 32-33 0.35771999678841127 1104 34-35 0.1958160128963371 1104 36-37 0.20906603961597625 1104 38-39 0.3378529815167397 1104 40-41 0.3946316834434782 1104 42-43 0.1651517835874543 1104 44-45 0.12162337596725337 1104 46-47 -0.08614296941132693 1104 48-49 -0.03961777579058179 1104 50-51 0.02757610165334512 1104 52-53 0.17085983324223264 1104 54-55 0.2452632887198689 1104 56-57 0.7332831558513462 1104 58-59 -6.168039208792333 1104 60-61 -8.284380657343561 1104 62-63 -2.3693156031771423 1104 64-65 -0.1623001247183744 1104 66-67 -1.5847948548627642 1104 68-69 -3.6368614796615173 1104 70-71 -4.0046717822770574 1104 72-73 -7.018936423634093 1104 74-75 -8.001294132049246 1104 76-77 -8.698067964236618 1104 78-79 -11.64939213114933 1104 80-81 -7.628803464961699 1104 82-83 -3.851250328676766 1104 84-85 -2.9732363172412732 1104 86-87 -2.597133772083442 1104 88-89 -2.9250240437611374 1104 90-91 -8.706444884274397 1104 92-93 -14.200696533158204 1104 94-95 -12.367182470929151 1104 96-97 -12.053481726792391 1104 98-99 -11.266639646114395 1104 100-101 -10.990754182340016 1105 1 0.101903678315729 1105 2 0.10710496501393862 1105 3 0.16003624550357642 1105 4 0.11074238270460768 1105 5 0.2598397395648675 1105 6 0.1443096527789578 1105 7 0.1195956685986701 1105 8 0.1401051380965228 1105 9 0.1768205194205521 1105 10-11 0.07297528357971572 1105 12-13 0.24202088256690502 1105 14-15 0.25649979778575727 1105 16-17 0.3211818448512531 1105 18-19 0.3741697157238164 1105 20-21 0.4825114966933981 1105 22-23 0.3554330249510187 1105 24-25 0.3745947315712854 1105 26-27 0.5483108838724107 1105 28-29 0.2681543556870807 1105 30-31 0.5022244493531076 1105 32-33 0.4028336748228796 1105 34-35 0.46967006496821995 1105 36-37 0.18082071508352016 1105 38-39 0.6120783207173233 1105 40-41 0.6008977267144644 1105 42-43 0.5046824033160959 1105 44-45 0.6458716341264079 1105 46-47 0.35057778599754386 1105 48-49 0.16928853150681533 1105 50-51 0.18660158753525735 1105 52-53 0.06474963888946661 1105 54-55 0.05704821721272424 1105 56-57 0.8720024444468635 1105 58-59 -6.939193407545542 1105 60-61 -8.668804933104468 1105 62-63 -2.560560561006259 1105 64-65 -0.272806175323435 1105 66-67 -1.6174085328972296 1105 68-69 -4.053188945741457 1105 70-71 -4.768734855251022 1105 72-73 -6.144944857810845 1105 74-75 -6.817383240960137 1105 76-77 -8.582872694709664 1105 78-79 -11.68999994193041 1105 80-81 -7.919205005115714 1105 82-83 -4.001387842428141 1105 84-85 -2.9974524888584355 1105 86-87 -2.9823097896852033 1105 88-89 -3.0156960276261913 1105 90-91 -8.391726329085545 1105 92-93 -11.309511164621352 1105 94-95 -11.140784414456837 1105 96-97 -11.391664711757555 1105 98-99 -10.876105176000717 1105 100-101 -10.880060196274744 1106 1 0.17745612151523105 1106 2 0.20119926940236255 1106 3 0.06436242041798579 1106 4 0.044791557930253134 1106 5 0.03818552232241501 1106 6 0.022896643130650318 1106 7 0.07667627862046089 1106 8 0.13425390871588405 1106 9 0.06588058789238715 1106 10-11 0.19666420359217085 1106 12-13 0.138375535539204 1106 14-15 0.32300306575961457 1106 16-17 0.19181287504110855 1106 18-19 0.3656729836976709 1106 20-21 0.25567520662180954 1106 22-23 0.19204600347575962 1106 24-25 0.2389738165386106 1106 26-27 0.3111454621481613 1106 28-29 0.2871202753882969 1106 30-31 0.28232871328089715 1106 32-33 0.48181749057140877 1106 34-35 0.35764080882130855 1106 36-37 0.2365630120022857 1106 38-39 0.2686881868860169 1106 40-41 0.315607499512474 1106 42-43 0.46240104632884993 1106 44-45 0.37727530670655085 1106 46-47 0.3124319721164355 1106 48-49 0.12013275808633495 1106 50-51 0.044650918378714266 1106 52-53 -0.04110548093624544 1106 54-55 -0.14774870155478936 1106 56-57 0.7761146448825968 1106 58-59 -6.384591011031382 1106 60-61 -8.542855664508146 1106 62-63 -2.3201388367610036 1106 64-65 -0.13760542835798262 1106 66-67 -1.038024001306816 1106 68-69 -3.4254889768650187 1106 70-71 -4.3518269809902055 1106 72-73 -6.062739753546918 1106 74-75 -6.60340098138964 1106 76-77 -7.766731238127031 1106 78-79 -11.351449521762344 1106 80-81 -7.093784837048489 1106 82-83 -3.4545624456027433 1106 84-85 -2.4724875028640376 1106 86-87 -2.380769173438704 1106 88-89 -2.6010173783887183 1106 90-91 -7.641613506178604 1106 92-93 -11.38762741111995 1106 94-95 -11.756793595907194 1106 96-97 -12.145975324891698 1106 98-99 -11.594401939150421 1106 100-101 -11.284281329172348 1107 1 0.06243176782470883 1107 2 -0.05422604297312006 1107 3 0.03182541410803097 1107 4 0.10134326566218022 1107 5 -0.06293433113913949 1107 6 -0.11080779405823904 1107 7 0.010038158404221065 1107 8 0.10755703038918085 1107 9 0.12197436298203712 1107 10-11 0.11155117749000709 1107 12-13 0.1316760404545505 1107 14-15 0.21537402688291962 1107 16-17 0.1845454245364948 1107 18-19 0.08283415814621975 1107 20-21 0.11256828232342997 1107 22-23 0.30202715092412546 1107 24-25 0.06337746334765626 1107 26-27 0.16261471226859214 1107 28-29 0.340499164098766 1107 30-31 0.10617332543574065 1107 32-33 0.376674371752749 1107 34-35 0.2620756188387361 1107 36-37 0.18523887300486308 1107 38-39 0.24767086275890904 1107 40-41 1.870638222811749E-4 1107 42-43 0.3392171655578622 1107 44-45 0.5386192498039648 1107 46-47 0.3075820491323853 1107 48-49 0.4235369222997889 1107 50-51 0.2748185693031999 1107 52-53 0.3423298464855975 1107 54-55 0.3853681545294734 1107 56-57 1.1251842090139803 1107 58-59 -6.41787141448788 1107 60-61 -9.043887499747548 1107 62-63 -2.6576530356465007 1107 64-65 -0.628351620677801 1107 66-67 -1.9416321536972845 1107 68-69 -4.42479613072444 1107 70-71 -5.071804143829864 1107 72-73 -6.063688328305908 1107 74-75 -6.689471503016552 1107 76-77 -8.934016411219623 1107 78-79 -12.239758560876927 1107 80-81 -8.181258892488074 1107 82-83 -4.171881220246359 1107 84-85 -3.2728050821250427 1107 86-87 -3.0640760418336193 1107 88-89 -3.094989914324536 1107 90-91 -7.951735007008235 1107 92-93 -11.118116209611747 1107 94-95 -11.776193287843418 1107 96-97 -12.20018087122913 1107 98-99 -11.459047160296205 1107 100-101 -11.099199228391482 1108 1 -0.05065291452093135 1108 2 -0.03282273039151562 1108 3 -0.06415070916114018 1108 4 -0.04568570930832294 1108 5 -0.12122530406195864 1108 6 -0.00812795781351383 1108 7 0.030499323489145524 1108 8 -0.15206644040124928 1108 9 -0.08087366570361354 1108 10-11 0.11094968714308351 1108 12-13 -0.08842408051808803 1108 14-15 -0.019140875836789917 1108 16-17 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1.7394475184802118 2312 84-85 1.1026793035178848 2312 86-87 0.7843163254810612 2312 88-89 0.8747709904868124 2312 90-91 2.3095679396357305 2312 92-93 4.313167261980432 2312 94-95 8.25676559906033 2312 96-97 8.881030611302734 2312 98-99 9.451169087313694 2312 100-101 8.674809363065618 2313 1 -0.30326358572250456 2313 2 -0.22873732889286913 2313 3 -0.29716208185604387 2313 4 -0.29603778455943086 2313 5 -0.3892940716657236 2313 6 -0.17707624925211007 2313 7 -0.4416349168254641 2313 8 -0.46521146883299025 2313 9 -0.5856645462903174 2313 10-11 -0.5364183842816885 2313 12-13 -0.5816648999898462 2313 14-15 -0.2983837147590478 2313 16-17 -0.40622257569832243 2313 18-19 -0.5882854814088034 2313 20-21 -0.4276886227809129 2313 22-23 -0.5097999499593726 2313 24-25 -0.37737659459357786 2313 26-27 -0.576002138827711 2313 28-29 -0.6515141030942786 2313 30-31 -0.7508370799180994 2313 32-33 -0.5858909368855976 2313 34-35 -0.6449058012205526 2313 36-37 -0.8023334067802779 2313 38-39 -0.9662072228908016 2313 40-41 -1.1879164907287816 2313 42-43 -1.068764430614607 2313 44-45 -1.0219449728031051 2313 46-47 -0.8266026202151195 2313 48-49 -0.8050095569041815 2313 50-51 -0.6051397505770453 2313 52-53 -0.6791721054355051 2313 54-55 -0.6911148652245558 2313 56-57 0.2867724563943028 2313 58-59 3.706849005835579 2313 60-61 3.537497336907478 2313 62-63 0.7072560459232555 2313 64-65 0.8521042189417969 2313 66-67 1.199273068058563 2313 68-69 -0.03711965063990519 2313 70-71 2.6106707600416925 2313 72-73 7.764717627254864 2313 74-75 9.095116759039861 2313 76-77 7.79889253826525 2313 78-79 5.739390739073173 2313 80-81 2.9916372887910967 2313 82-83 1.5058254192206029 2313 84-85 0.9501281563028563 2313 86-87 0.6501274863004589 2313 88-89 0.6432794801396398 2313 90-91 2.0556452360279565 2313 92-93 4.0395927780309755 2313 94-95 7.546342228314966 2313 96-97 8.153645921456302 2313 98-99 8.459229352075749 2313 100-101 7.509396195601001 2314 1 -0.29035838815171644 2314 2 -0.3881341004573997 2314 3 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62-63 1.2352580571228735 2314 64-65 1.4331976279908822 2314 66-67 2.0621565762216676 2314 68-69 0.5088684661937037 2314 70-71 2.9742946657124847 2314 72-73 8.517635509842872 2314 74-75 9.848855552845897 2314 76-77 8.57434341504312 2314 78-79 6.200440835032374 2314 80-81 3.2056999120263683 2314 82-83 1.9206849638744963 2314 84-85 1.4836294182320309 2314 86-87 1.4988773877122448 2314 88-89 1.763325750876028 2314 90-91 2.871063447243351 2314 92-93 4.8234588163620735 2314 94-95 8.5474920955495 2314 96-97 9.302702760849527 2314 98-99 9.740438109338413 2314 100-101 8.626628430004347 2315 1 -0.7123554244049117 2315 2 -0.4876001162296042 2315 3 -0.3802974948301667 2315 4 -0.42976355010901557 2315 5 -0.8565929018677778 2315 6 -0.8281301581223914 2315 7 -0.729698342233803 2315 8 -0.7696847870780203 2315 9 -1.1119822732283922 2315 10-11 -0.9611184360909206 2315 12-13 -0.7208391562546694 2315 14-15 -0.8258872596012949 2315 16-17 -0.9345165521932941 2315 18-19 -0.9824753486135549 2315 20-21 -1.0618210226391227 2315 22-23 -0.7238651341548277 2315 24-25 -0.8361923446003985 2315 26-27 -0.8697008408700881 2315 28-29 -0.8158775312294253 2315 30-31 -0.8625394904108319 2315 32-33 -0.8108360600006961 2315 34-35 -0.7088043096600032 2315 36-37 -0.9169626980845109 2315 38-39 -0.8275858256775948 2315 40-41 -0.4777891490877977 2315 42-43 -0.09762967207289819 2315 44-45 -0.4103257297632652 2315 46-47 -0.4329782235200028 2315 48-49 -0.33399682274008313 2315 50-51 -0.27756633144034737 2315 52-53 -0.1796670199502728 2315 54-55 -0.24953931918250305 2315 56-57 1.0221077166675272 2315 58-59 4.4482064077388905 2315 60-61 4.04164480234526 2315 62-63 1.5668329144641397 2315 64-65 1.5003317978145354 2315 66-67 2.397511554138241 2315 68-69 6.36195460017132 2315 70-71 5.146465827257352 2315 72-73 9.134073488515195 2315 74-75 10.29020340316497 2315 76-77 9.141226816889848 2315 78-79 6.614309957571798 2315 80-81 3.4028035418928333 2315 82-83 1.8435496986632458 2315 84-85 1.3637795483351418 2315 86-87 1.3810085159407954 2315 88-89 1.4188446097990592 2315 90-91 2.6966839429785736 2315 92-93 4.632043968279934 2315 94-95 8.784388952062685 2315 96-97 9.470434158033623 2315 98-99 9.46309561608469 2315 100-101 8.537038125631272 2316 1 -0.5808711352509981 2316 2 -0.5723787942297172 2316 3 -0.7157539507583977 2316 4 -0.5495808723111466 2316 5 -0.6515233294856486 2316 6 -0.7201884594866357 2316 7 -0.5839114611089613 2316 8 -0.6598074956830047 2316 9 -0.9599029352894206 2316 10-11 -0.7054464300462868 2316 12-13 -0.5807867713380546 2316 14-15 -0.9507055752368032 2316 16-17 -0.9821696637761548 2316 18-19 -1.083220530461709 2316 20-21 -0.9077573104456746 2316 22-23 -0.9908028170409295 2316 24-25 -2.1704534758610734 2316 26-27 -1.6227603664006125 2316 28-29 -1.0636754205929932 2316 30-31 -0.6377276552367377 2316 32-33 -0.8200258527754798 2316 34-35 -0.8610688036392276 2316 36-37 -0.8517450435984983 2316 38-39 -1.0372262597688078 2316 40-41 -1.0336240212339902 2316 42-43 -0.9314482091835146 2316 44-45 -0.8493232237474686 2316 46-47 -0.689682830558354 2316 48-49 -0.9350731490448467 2316 50-51 -1.020679060846895 2316 52-53 -0.951567952923071 2316 54-55 -0.8389910507831573 2316 56-57 0.2582660564160406 2316 58-59 3.529937844599612 2316 60-61 3.4851398077610796 2316 62-63 1.0311170264379683 2316 64-65 0.5435749910156069 2316 66-67 5.627772674004699 2316 68-69 8.371548175541577 2316 70-71 5.627184674219478 2316 72-73 9.031648015601743 2316 74-75 9.671643717759324 2316 76-77 8.44086946573216 2316 78-79 5.9338077431767715 2316 80-81 3.0152071450293754 2316 82-83 1.5995154618871261 2316 84-85 1.0289059778643939 2316 86-87 0.8436052154631639 2316 88-89 1.0373387784520034 2316 90-91 2.5596282373595827 2316 92-93 4.183691821431257 2316 94-95 8.105008355988417 2316 96-97 8.832591895312866 2316 98-99 9.259779328976038 2316 100-101 8.143718626777458 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 7.0 4 25.0 5 88.0 6 202.0 7 419.0 8 913.0 9 1527.0 10 2543.0 11 3978.0 12 5346.0 13 7377.0 14 9302.0 15 12973.0 16 16899.0 17 21415.0 18 21461.0 19 19672.0 20 20003.0 21 19638.0 22 18734.0 23 18518.0 24 19083.0 25 20618.0 26 26710.0 27 37923.0 28 52082.0 29 68158.0 30 80259.0 31 87259.0 32 90661.0 33 93600.0 34 95406.0 35 91942.0 36 76707.0 37 47907.0 38 15145.0 39 641.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.530958882289 20.316028313848932 14.731093554988185 21.42191924887388 2 15.738122363881018 22.183713230070918 39.45375949817253 22.62440490787553 3 18.857716301300695 27.70088190954319 30.678887559700375 22.762514229455743 4 12.297344863687076 18.11452113350242 37.51539396330423 32.07274003950627 5 12.245709189194082 39.815555122940616 33.70243436451632 14.236301323348982 6 29.33634712674672 37.833090981150825 17.954722519569902 14.875839372532553 7 27.066410530421003 35.14275553979085 20.636733231325234 17.154100698462912 8 26.76815022196323 34.85790914799781 19.7557228147073 18.61821781533166 9 27.673391902028794 16.24399058581665 20.293700079899306 35.78891743225525 10-11 24.685764078972728 27.861965124812127 26.814135028923914 20.63813576729123 12-13 26.22579381273521 25.695499488300587 27.667284084112342 20.41142261485186 14-15 22.939603072554675 26.286512375366357 27.18526809749724 23.588616454581732 16-17 20.757758512262235 29.943735686215607 27.71750392031424 21.581001881207918 18-19 20.63550449633615 27.535285696963776 31.559637759450993 20.26957204724908 20-21 22.0531588276971 26.535075614785807 31.28320277684024 20.128562780676855 22-23 21.375010066588786 26.805176895979788 31.11150522874457 20.708307808686854 24-25 20.78273270107615 27.099437990265496 31.627864679710555 20.4899646289478 26-27 20.877028757881916 27.316662451516343 31.460841404328228 20.345467386273512 28-29 20.456262685400265 27.246271634743724 31.81097973141111 20.486485948444905 30-31 20.509011568979496 27.442299895748874 31.635240501563764 20.413448033707866 32-33 20.276138459227568 27.559191916998298 31.176626544835283 20.98804307893884 34-35 20.831287945247993 27.345824490093236 31.254464978507023 20.568422586151748 36-37 21.052941213738848 27.09149608052372 31.094828608330232 20.760734097407198 38-39 20.718775214853746 27.20513865991721 31.360026138597014 20.716059986632025 40-41 20.627095863258074 27.24800991985374 31.582696371922108 20.542197844966083 42-43 20.624853204254144 27.32756393053727 31.077208113488552 20.970374751720026 44-45 20.560836058815195 27.650664049117957 30.72116996824457 21.06732992382227 46-47 20.663105640995933 27.74970662947246 30.111605253832824 21.475582475698783 48-49 20.673253812695815 27.84957122304502 29.930753958646072 21.54642100561309 50-51 20.843747468590156 27.960567253026035 29.724035032223288 21.471650246160515 52-53 20.96235248758493 28.068289910372545 29.60918882765699 21.36016877438554 54-55 20.412438962915967 27.89322905236236 29.899946921323643 21.79438506339802 56-57 20.811198291908863 28.289262624450135 29.29283866839941 21.606700415241594 58-59 19.472142634089856 27.578917616595668 30.97390800903544 21.975031740279043 60-61 20.861591664094977 27.790676566158982 29.55334289396772 21.794388875778314 62-63 20.674043127684623 27.76800523374025 29.628091358808906 21.92986027976622 64-65 20.0380948756321 27.57175524133022 30.246098877225126 22.14405100581255 66-67 20.583214245395496 27.619131534577722 29.74775385005108 22.049900369975706 68-69 21.57316276398606 26.949910367389073 29.506450990376408 21.970475878248454 70-71 20.666864953902845 27.299064179842514 29.805809569317347 22.228261296937298 72-73 20.323399092439516 27.287293968834646 29.746023199768405 22.643283738957432 74-75 20.763429296271784 27.249522023678214 29.282070372821533 22.704978307228473 76-77 19.066204297036034 27.612416628651882 30.356298840613793 22.96508023369829 78-79 19.64960824002199 27.835381298657637 29.42177635718878 23.093234104131596 80-81 19.768743917617567 28.21411072005907 28.869322713849893 23.147822648473472 82-83 20.164124225153678 27.996762396749364 28.35345976997453 23.48565360812242 84-85 19.965832398685958 27.523604464771672 28.49099277422192 24.019570362320447 86-87 20.132511935485038 27.445598077291994 28.62769973613439 23.794190251088583 88-89 20.23282118102684 27.27591074434008 28.586694898383914 23.904573176249162 90-91 19.80972341378741 27.40790888924762 28.730077685012034 24.052290011952934 92-93 19.559570991871688 27.3126715114764 29.008490880291504 24.11926661636041 94-95 18.842805118132794 27.175561672216737 29.288819726308695 24.692813483341773 96-97 18.70139927916616 26.873629533565975 29.54250193039577 24.882469256872096 98-99 18.838387415939838 26.8845813729769 29.079708340093184 25.197322870990075 100-101 18.458877180518122 26.963221522607068 29.470206948494322 25.107694348380484 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1390.0 1 1002.0 2 615.5 3 708.5 4 809.5 5 878.0 6 1182.0 7 1815.0 8 2474.5 9 2805.5 10 2781.5 11 2779.5 12 2972.0 13 3087.0 14 3156.0 15 3202.5 16 3174.0 17 3191.0 18 3210.0 19 3309.0 20 3523.5 21 3848.5 22 4347.5 23 4849.5 24 5368.5 25 6165.5 26 7233.5 27 8605.0 28 10217.5 29 11927.0 30 13776.0 31 16035.5 32 18878.0 33 21882.0 34 24574.0 35 27211.0 36 29930.5 37 32126.0 38 33446.5 39 34766.5 40 36062.5 41 37375.0 42 38674.5 43 39707.5 44 40698.5 45 41374.5 46 41873.5 47 42387.0 48 43411.0 49 42601.5 50 39699.5 51 37046.5 52 34447.5 53 32242.0 54 30248.0 55 27761.0 56 24671.0 57 21614.0 58 18829.5 59 15862.0 60 13115.5 61 10608.5 62 8284.5 63 6301.0 64 4637.5 65 3449.0 66 2603.0 67 1878.0 68 1310.0 69 902.5 70 681.0 71 553.0 72 460.5 73 397.5 74 303.5 75 197.0 76 123.5 77 75.0 78 42.0 79 23.5 80 17.5 81 14.0 82 8.0 83 3.5 84 1.0 85 1.0 86 1.0 87 0.5 88 1.0 89 1.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02171668592514439 2 0.005429171481286098 3 0.003528961462835964 4 0.0 5 0.06750269875065715 6 0.0 7 0.0 8 2.714585740643049E-4 9 0.0 10-11 0.0 12-13 0.0 14-15 1.8097238270953662E-4 16-17 0.0 18-19 0.0017192376357405977 20-21 0.0 22-23 0.0 24-25 0.0 26-27 4.5243095677384154E-5 28-29 0.002895558123352586 30-31 0.010586884388507892 32-33 6.334033394833782E-4 34-35 0.0011310773919346038 36-37 0.02479321643120652 38-39 0.02366213903927191 40-41 0.025879050727463736 42-43 0.02619575239720542 44-45 0.026286238588560194 46-47 0.028005476224300795 48-49 0.028276934798365093 50-51 0.024657487144174362 52-53 0.021173768777015784 54-55 0.016061298965471375 56-57 0.15265020481549413 58-59 0.7554239685252832 60-61 0.307743536797567 62-63 3.6194476541907323E-4 64-65 0.1430134254362113 66-67 1.4124894470479332 68-69 2.7072111160476355 70-71 2.1913493391341015 72-73 1.852704767988881 74-75 1.5582627013204648 76-77 1.1188165130060328 78-79 0.42152992242618814 80-81 2.2621547838692075E-4 82-83 2.2621547838692075E-4 84-85 4.5243095677384154E-5 86-87 0.002986044314707354 88-89 9.048619135476831E-5 90-91 0.2246772131338897 92-93 0.17178803428702763 94-95 1.295174099956476 96-97 1.2707428282906887 98-99 1.0006415470967054 100-101 0.403251711772525 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1105141.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 50.72285660644758 #Duplication Level Percentage of deduplicated Percentage of total 1 74.98958930744001 38.03686185417675 2 13.327218501125898 13.519891859908082 3 4.8332318720565794 7.354659815761141 4 2.264677469531182 4.594836421875308 5 1.2091726698909795 3.0666345973657765 6 0.7138544277072788 2.1725241464684397 7 0.49737322246831606 1.7659733450203003 8 0.339280886615208 1.3767436608873276 9 0.2584632918686283 1.179899684233456 >10 1.3811636249427832 13.289358081937284 >50 0.12268955380573517 4.2722067439408615 >100 0.05971981127637526 5.369478605781422 >500 0.0016044125719755055 0.5839495011529244 >1k 0.0014261445084226714 1.2262216142977405 >5k 5.348041906585018E-4 2.190760067193102 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9806 0.887307592424858 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8250 0.7465110786768385 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6172 0.55848077304163 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3146 0.2846695580021011 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1952 0.17662904552450773 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1887 0.1707474430864478 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 1725 0.15608868008697535 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1694 0.15328360815497752 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 1106 0.10007772763837375 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 9.048619135476831E-5 0.0 14-15 0.0 0.0 0.0 9.048619135476831E-5 0.0 16-17 0.0 0.0 0.0 9.048619135476831E-5 0.0 18-19 0.0 0.0 0.0 1.8097238270953662E-4 0.0 20-21 0.0 0.0 0.0 6.334033394833781E-4 0.0 22-23 0.0 0.0 0.0 0.0018097238270953662 0.0 24-25 0.0 0.0 0.0 0.003845663132577653 0.0 26-27 0.0 0.0 0.0 0.007465110786768385 0.0 28-29 0.0 0.0 0.0 0.018413939940695352 0.0 30-31 0.0 0.0 0.0 0.04574076972983538 0.0 32-33 0.0 0.0 0.0 0.10089210336056667 0.0 34-35 0.0 0.0 0.0 0.18762311777411209 0.0 36-37 0.0 0.0 0.0 0.3314509189325163 0.0 38-39 0.0 0.0 0.0 0.5492511815234435 0.0 40-41 0.0 0.0 0.0 0.7939258429467371 0.0 42-43 0.0 0.0 0.0 1.024167956848945 0.0 44-45 0.0 0.0 0.0 1.2830941934106146 0.0 46-47 0.0 0.0 0.0 1.5926474540352769 0.0 48-49 0.0 0.0 0.0 1.9053224882616788 0.0 50-51 0.0 0.0 0.0 2.2240148542131726 0.0 52-53 0.0 0.0 0.0 2.5099512188942406 0.0 54-55 0.0 0.0 0.0 2.808057976312525 0.0 56-57 0.0 0.0 0.0 3.073815920321479 0.0 58-59 0.0 0.0 0.0 3.260579419277721 0.0 60-61 0.0 0.0 0.0 3.409564933343347 0.0 62-63 9.048619135476831E-5 0.0 0.0 3.5299568109408663 4.5243095677384154E-5 64-65 9.048619135476831E-5 0.0 0.0 3.643471737995423 9.048619135476831E-5 66-67 9.048619135476831E-5 0.0 0.0 3.7405634213190897 9.048619135476831E-5 68-69 9.048619135476831E-5 0.0 0.0 3.8538521328952595 9.048619135476831E-5 70-71 9.048619135476831E-5 0.0 0.0 4.074276495035475 9.048619135476831E-5 72-73 9.048619135476831E-5 0.0 0.0 4.341527461201784 9.048619135476831E-5 74-75 1.3572928703215246E-4 0.0 0.0 4.631535704493816 9.048619135476831E-5 76-77 1.8097238270953662E-4 0.0 0.0 5.047455483056009 9.048619135476831E-5 78-79 1.8097238270953662E-4 0.0 0.0 5.631770063729425 9.048619135476831E-5 80-81 1.8097238270953662E-4 0.0 0.0 6.308380559584705 9.048619135476831E-5 82-83 1.8097238270953662E-4 0.0 0.0 6.936128512108409 9.048619135476831E-5 84-85 1.8097238270953662E-4 0.0 0.0 7.441222432250726 9.048619135476831E-5 86-87 1.8097238270953662E-4 0.0 0.0 7.879537543173224 9.048619135476831E-5 88-89 1.8097238270953662E-4 0.0 0.0 8.309889869256502 9.048619135476831E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6165 0.0 36.0361 1 GTATCAA 9405 0.0 32.749477 1 GTACATG 8985 0.0 26.720814 1 TACATGG 8865 0.0 26.708857 2 ATCAACG 11665 0.0 26.360514 3 TCAACGC 11805 0.0 26.047894 4 TATCAAC 11825 0.0 25.926416 2 ACATGGG 8830 0.0 25.797487 3 CAACGCA 12060 0.0 25.536236 5 AACGCAG 12250 0.0 25.04502 6 CATGGGA 6300 0.0 23.580935 4 ATGGGGG 1160 0.0 23.580933 5 CATGGGG 2260 0.0 23.163572 4 GAGTACT 6985 0.0 22.737873 12-13 CGCAGAG 13540 0.0 22.589287 8 ACGCAGA 13635 0.0 22.466461 7 AGTACTT 7170 0.0 21.888268 12-13 GTAAGGT 415 0.0 21.59218 4 GCAGAGT 14040 0.0 21.516293 9 ATAACGC 110 0.0029990575 21.437212 3 >>END_MODULE