FastQCFastQC Report
Fri 27 May 2016
SRR939770_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939770_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1176166
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT97120.8257337824762832No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74070.6297580443576842No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54290.4615845042281447No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA28300.24061229452305202No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA20090.17080922250770725No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA16850.14326209055524475No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16390.13935107799409266No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16310.138670901896501No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN11860.10083610646796455No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA104950.033.298511
GGTATCA67650.032.816531
GTACATG92100.029.1604961
TACATGG92700.028.9210172
TATCAAC127900.027.4338472
ATCAACG127650.027.3770333
TCAACGC128500.027.3423544
ACATGGG95500.027.0387973
CAACGCA129700.026.9828835
ACGTCCG350.00875505626.9090488-89
AACGCAG132400.026.5036856
CATGGGA64700.025.5892074
CAACGCG950.001298358424.7572155
ACGCAGA146300.023.9212577
CGCAGAG146850.023.6384688
ATGGGAT25900.023.6101635
GAGTACT68700.023.41465812-13
CATGGGG26250.022.9350174
GCAGAGT146800.022.2687429
ATGGGAG22450.022.2098145
Produced by FastQC (version 0.11.5)