FastQCFastQC Report
Fri 27 May 2016
SRR939770_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939770_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1176166
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT104460.8881399394303185No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT88470.7521897419241841No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65390.5559589377689884No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA33870.2879695553178718No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20510.1743801470200635No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA19830.16859865019053433No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19380.16477265964158122No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA17440.1482783892749833No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT11870.1009211284801635No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA70350.034.5926251
GTACACG1701.09139364E-1030.6954521
GTATCAA105100.030.3317051
GTACATG96650.026.9464021
TACATGG96950.026.614972
TAAGGTG3850.025.8689085
ACATGGG97750.025.4719413
TATCAAC131500.024.2392082
ATCAACG131500.024.200053
TCAACGC132650.024.0260014
CAACGCA134900.023.6604295
CATGGGG26000.023.3483454
AACGCAG137600.023.1616956
GAGTACT76850.022.37090912-13
CATGGGA69250.022.2578434
TACAGCG1100.00290343821.557427
AGTACTT78450.021.37056412-13
GTACTTT82450.020.9377614-15
ACGCAGA152400.020.8812757
GCACCGT2509.660653E-920.8675866
Produced by FastQC (version 0.11.5)