##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939770_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1176166 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.38707291317722 31.0 31.0 34.0 30.0 34.0 2 31.598177468146503 31.0 31.0 34.0 30.0 34.0 3 31.620609675845078 31.0 31.0 34.0 30.0 34.0 4 35.43802235398745 37.0 35.0 37.0 33.0 37.0 5 35.20048870652612 37.0 35.0 37.0 32.0 37.0 6 35.244433183751276 37.0 35.0 37.0 32.0 37.0 7 35.14629142484989 37.0 35.0 37.0 32.0 37.0 8 35.115151262661904 37.0 35.0 37.0 32.0 37.0 9 36.66140493773838 39.0 35.0 39.0 32.0 39.0 10-11 36.536463815481824 38.0 35.0 39.0 32.0 39.0 12-13 36.4515319266158 38.0 35.0 39.0 32.0 39.0 14-15 37.396839816828574 39.0 36.0 41.0 32.0 41.0 16-17 37.33707231802313 39.0 36.0 41.0 32.0 41.0 18-19 37.398553860594504 39.0 36.0 41.0 32.0 41.0 20-21 37.36009415337631 39.0 36.0 41.0 32.0 41.0 22-23 37.220717143668494 39.0 36.0 40.0 31.0 41.0 24-25 37.06484543848402 39.0 36.0 40.0 31.0 41.0 26-27 36.74320419056494 39.0 35.5 40.0 30.5 41.0 28-29 36.49489740393788 38.0 35.0 40.0 30.0 41.0 30-31 36.183261121304305 38.0 35.0 40.0 30.0 41.0 32-33 36.0585712390938 38.0 35.0 40.0 30.0 41.0 34-35 35.86113822368611 38.0 34.5 40.0 29.5 41.0 36-37 35.81007187760911 38.0 35.0 40.0 29.0 41.0 38-39 35.672179352234295 38.0 34.5 40.0 29.0 41.0 40-41 35.53376181593414 38.0 34.0 40.0 27.5 41.0 42-43 35.244044633155525 38.0 34.0 40.0 27.0 41.0 44-45 34.90049151225252 38.0 33.5 40.0 25.5 41.0 46-47 34.4715716148911 38.0 33.0 40.0 24.0 41.0 48-49 34.07648877794461 38.0 33.0 40.0 23.0 41.0 50-51 33.81655098004873 38.0 33.0 40.0 22.5 41.0 52-53 33.54726841279207 37.0 33.0 40.0 21.5 41.0 54-55 33.27813250850645 37.0 32.5 40.0 20.0 41.0 56-57 32.93461637217876 37.0 31.5 40.0 18.5 41.0 58-59 32.58152590705734 36.0 31.0 39.0 17.5 41.0 60-61 32.271071430393334 36.0 31.0 39.0 14.5 40.5 62-63 31.977969521309063 35.5 31.0 39.0 13.0 40.0 64-65 31.54309638265347 35.0 30.5 39.0 9.5 40.0 66-67 30.662723204037526 35.0 29.0 38.0 7.5 40.0 68-69 28.813463830785793 34.0 25.5 37.0 3.5 39.5 70-71 28.181346850699647 33.0 24.0 36.5 2.0 39.0 72-73 28.713891151419105 33.5 26.0 36.0 2.0 39.0 74-75 28.793186505986398 33.0 26.5 36.0 2.0 39.0 76-77 28.303418054934426 32.5 26.5 35.0 2.0 37.5 78-79 28.803987277306096 34.0 28.0 35.0 2.0 37.0 80-81 28.614259806864 34.0 28.0 35.0 2.0 37.0 82-83 28.168816731651823 33.5 26.5 35.0 2.0 36.5 84-85 27.81472895832731 33.0 26.0 35.0 2.0 36.0 86-87 27.429952914809643 33.0 26.0 35.0 2.0 36.0 88-89 26.95648785970688 33.0 25.5 35.0 2.0 35.0 90-91 26.463563391562076 32.0 25.0 35.0 2.0 35.0 92-93 25.98903811196719 32.0 24.0 34.5 2.0 35.0 94-95 25.211400006461673 32.0 20.0 34.0 2.0 35.0 96-97 24.694307606239256 31.0 18.5 34.0 2.0 35.0 98-99 24.040949576845446 31.0 4.5 34.0 2.0 35.0 100-101 22.621033085465825 30.0 2.0 34.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.3194726113669084 1101 2 0.3494610606873181 1101 3 0.4491310642895776 1101 4 0.3795555200558809 1101 5 0.22200123786734594 1101 6 0.1557129653255771 1101 7 0.3074224115517481 1101 8 0.13071736002629564 1101 9 0.38148820816963536 1101 10-11 0.36396616310436514 1101 12-13 0.3518701556377337 1101 14-15 0.4425311246692871 1101 16-17 0.4980138959570226 1101 18-19 0.4127362471094358 1101 20-21 0.3840968310100834 1101 22-23 0.2561023943464491 1101 24-25 0.35302772657315273 1101 26-27 0.3681586163841146 1101 28-29 0.19417372239928454 1101 30-31 -0.0763562834952225 1101 32-33 0.0325369609977173 1101 34-35 0.20190677414432656 1101 36-37 0.30709724176222863 1101 38-39 0.4260210455822886 1101 40-41 0.5740495231505776 1101 42-43 0.272416315714068 1101 44-45 0.11644986658835421 1101 46-47 -0.028729325143750373 1101 48-49 0.28727507587080225 1101 50-51 0.1702998686394821 1101 52-53 0.1156016494273544 1101 54-55 -0.18996541037389392 1101 56-57 -0.15360444643946636 1101 58-59 0.08406259659926008 1101 60-61 -0.01916196533733938 1101 62-63 -0.3794291940096137 1101 64-65 -0.33219293212198764 1101 66-67 -0.18144384225101362 1101 68-69 -8.36675527621156 1101 70-71 -6.55683779608994 1101 72-73 -1.7066905747694037 1101 74-75 -0.6217067324849879 1101 76-77 -0.362397594225758 1101 78-79 -0.6997082054155221 1101 80-81 -0.5353147757424956 1101 82-83 -0.4099678185174547 1101 84-85 -0.41628421794613146 1101 86-87 -0.6982984584868959 1101 88-89 -0.5179218644295815 1101 90-91 -0.575722502043984 1101 92-93 -0.7387087746678738 1101 94-95 -0.2227147652377326 1101 96-97 -0.5181399645307927 1101 98-99 -0.6021970497224203 1101 100-101 -0.4706310248804719 1102 1 0.2183385777307265 1102 2 0.197222606965628 1102 3 0.19070924105691844 1102 4 0.20690325830215528 1102 5 0.28373421684022304 1102 6 0.264134638235646 1102 7 0.31275134744818445 1102 8 0.26125749916488417 1102 9 0.41472528493638094 1102 10-11 0.3401430386793507 1102 12-13 0.398735478620857 1102 14-15 0.3220866508284246 1102 16-17 0.2900737814713281 1102 18-19 0.43383627777524225 1102 20-21 0.4585955803261186 1102 22-23 0.38432643814444845 1102 24-25 0.18849288480271298 1102 26-27 0.3458815598207323 1102 28-29 0.17903135187213337 1102 30-31 0.1483278887383932 1102 32-33 0.1527784993390071 1102 34-35 0.18944352801813835 1102 36-37 0.38243554275616276 1102 38-39 0.5379226865037054 1102 40-41 0.3752899851820999 1102 42-43 0.3967082963044106 1102 44-45 0.4655985145869579 1102 46-47 0.5007523174612842 1102 48-49 0.7529293699859707 1102 50-51 0.6128529625790762 1102 52-53 0.22050464625855426 1102 54-55 0.08966166933917208 1102 56-57 0.11436098237075498 1102 58-59 0.053654215212802114 1102 60-61 0.202126043730118 1102 62-63 0.0923233118679967 1102 64-65 0.10423338876262633 1102 66-67 0.45875377182879973 1102 68-69 -4.631934851580301 1102 70-71 0.12917476185941723 1102 72-73 0.5017347058828747 1102 74-75 0.2635415102439076 1102 76-77 -0.01516029798802876 1102 78-79 -0.2140767903988987 1102 80-81 -0.2980944509254364 1102 82-83 -0.23618550667624305 1102 84-85 -0.29184266637646417 1102 86-87 -0.4667652131423843 1102 88-89 -0.532750812351825 1102 90-91 -0.7651515167094551 1102 92-93 -0.7858766218679669 1102 94-95 -0.37987929030853707 1102 96-97 -0.6087544231590094 1102 98-99 -0.2882592141272369 1102 100-101 -0.19528851485395649 1103 1 0.10686301728819814 1103 2 0.25571361447743257 1103 3 0.1808590608339351 1103 4 0.1292300942296194 1103 5 0.2918525051309686 1103 6 0.19455392403298077 1103 7 0.17134771829842066 1103 8 0.14763950299184359 1103 9 0.17963225944390615 1103 10-11 0.36780414482214496 1103 12-13 0.3326379270983253 1103 14-15 0.3071452250930733 1103 16-17 0.3302977431615659 1103 18-19 0.27345253416127946 1103 20-21 0.290768936033615 1103 22-23 0.27017085927051454 1103 24-25 0.24305860554393632 1103 26-27 0.17671921271599444 1103 28-29 0.24660887072341353 1103 30-31 0.21894357524370633 1103 32-33 0.36380877766185904 1103 34-35 0.3527846942209081 1103 36-37 0.581980643002062 1103 38-39 0.723431064611674 1103 40-41 0.6708337906360171 1103 42-43 0.42035081026792653 1103 44-45 0.36832726715106645 1103 46-47 0.47933262542655086 1103 48-49 0.5966952049904322 1103 50-51 0.6832038052704661 1103 52-53 0.5300256019356127 1103 54-55 0.4948443541520646 1103 56-57 0.5064766035622839 1103 58-59 0.3281005911589574 1103 60-61 0.2565532418261185 1103 62-63 0.2927217977365437 1103 64-65 0.3128929919706138 1103 66-67 0.5269381347035846 1103 68-69 -3.7481280770334457 1103 70-71 0.8111421997072519 1103 72-73 0.9424186632379978 1103 74-75 0.5908123844581681 1103 76-77 0.4596389877231779 1103 78-79 0.40224347395685456 1103 80-81 0.40987505112513034 1103 82-83 0.263319974657648 1103 84-85 -7.373274409765429E-4 1103 86-87 -0.1999788523834809 1103 88-89 -0.08422920066596618 1103 90-91 -0.04582710528714884 1103 92-93 -0.261432399123553 1103 94-95 -0.4391608817255843 1103 96-97 -0.9509125341981921 1103 98-99 -0.3105520294672175 1103 100-101 -0.358489722943677 1104 1 0.16222311648211019 1104 2 0.3010347783934222 1104 3 0.17882819896173174 1104 4 0.27349078961562867 1104 5 0.22565349734808393 1104 6 0.1942501982841307 1104 7 0.05146292557560628 1104 8 0.11777360103757673 1104 9 0.05173313809400071 1104 10-11 0.20566567924185364 1104 12-13 0.1745288666186795 1104 14-15 0.11011582972276557 1104 16-17 0.2698681345577185 1104 18-19 0.1838586509337503 1104 20-21 0.19003033339446773 1104 22-23 0.2376791543890775 1104 24-25 0.25972043020590974 1104 26-27 0.2988624878665007 1104 28-29 0.21596245614680498 1104 30-31 0.30576118271883246 1104 32-33 0.3318897730482888 1104 34-35 0.23356017486516834 1104 36-37 0.3748369021850735 1104 38-39 0.5421116577178609 1104 40-41 0.20687880876920417 1104 42-43 0.0885318196938556 1104 44-45 0.22109568912551936 1104 46-47 0.27590135849401776 1104 48-49 0.2511950027382781 1104 50-51 0.01964853204935224 1104 52-53 0.06190186253648022 1104 54-55 0.13819352477921143 1104 56-57 0.2176956211033101 1104 58-59 0.033608939099394775 1104 60-61 -0.013688307022292179 1104 62-63 0.19629478018438462 1104 64-65 0.2949439817551429 1104 66-67 0.41485047542132136 1104 68-69 -4.038455141708262 1104 70-71 0.6479896932400457 1104 72-73 0.9833142580330971 1104 74-75 0.5286938660838025 1104 76-77 0.18132507569383094 1104 78-79 -0.0620162761957026 1104 80-81 -0.075979460766046 1104 82-83 -0.05582419985468334 1104 84-85 0.002680456014378052 1104 86-87 0.1741574057986952 1104 88-89 0.11010627936667206 1104 90-91 0.03800120139662155 1104 92-93 -0.043928274152566615 1104 94-95 0.36275815068896833 1104 96-97 -0.044079886390782974 1104 98-99 0.22322269082013335 1104 100-101 0.15190854005085086 1105 1 0.03646422902077617 1105 2 0.08388888451911214 1105 3 0.13604927852567172 1105 4 0.02011611839793659 1105 5 0.07577908253923482 1105 6 0.1369800426191503 1105 7 0.14794364528490433 1105 8 0.09474505418643986 1105 9 0.14098202561047657 1105 10-11 0.23196447274174403 1105 12-13 0.017270979978952994 1105 14-15 0.34347992752206125 1105 16-17 0.29352633054229926 1105 18-19 0.15092975889566418 1105 20-21 0.14372958236469913 1105 22-23 0.14303913310438787 1105 24-25 0.1583755244706211 1105 26-27 0.16235407324513318 1105 28-29 0.3437216082882273 1105 30-31 0.2225802931731451 1105 32-33 0.14489397484480548 1105 34-35 0.26810299514186653 1105 36-37 0.06768805823052304 1105 38-39 0.28906404562859933 1105 40-41 0.1684609064692424 1105 42-43 0.1532023343312119 1105 44-45 0.22532195476507866 1105 46-47 0.41277370824671067 1105 48-49 0.2635115877033414 1105 50-51 0.276526423541533 1105 52-53 0.19374908146551917 1105 54-55 0.22142369720727118 1105 56-57 0.24867870087678767 1105 58-59 0.3649811813257884 1105 60-61 0.2327946872815687 1105 62-63 0.33063888360813465 1105 64-65 0.30376837478302576 1105 66-67 0.7487447605369262 1105 68-69 2.019993322871475 1105 70-71 2.4077311614425945 1105 72-73 1.353609638262597 1105 74-75 0.9789074711921302 1105 76-77 0.761363578376475 1105 78-79 0.5173760348475263 1105 80-81 0.34279097612153464 1105 82-83 0.35826436320810373 1105 84-85 0.21367977501455115 1105 86-87 0.3110245860182701 1105 88-89 0.4814355662192398 1105 90-91 0.5643489331433464 1105 92-93 0.554302849469579 1105 94-95 0.9710343664253074 1105 96-97 0.6972828576801042 1105 98-99 0.828006025348202 1105 100-101 0.9168643439890687 1106 1 0.4884289871434788 1106 2 0.393971797957132 1106 3 0.43338208784529186 1106 4 0.3161980405115088 1106 5 0.3402273430287934 1106 6 0.4827645673993288 1106 7 0.4048088603553808 1106 8 0.3761477489465008 1106 9 0.49757506639555515 1106 10-11 0.48494394528675144 1106 12-13 0.4694373758400303 1106 14-15 0.5317182882585811 1106 16-17 0.6316680518100242 1106 18-19 0.597059120869865 1106 20-21 0.5194690429434132 1106 22-23 0.6785130771092156 1106 24-25 0.6055479034733082 1106 26-27 0.7794971671159274 1106 28-29 0.7028054250633176 1106 30-31 0.7179136626790665 1106 32-33 0.6598217665376183 1106 34-35 0.6444931956944302 1106 36-37 0.6231069074729518 1106 38-39 0.7040209464785576 1106 40-41 0.6414524479494261 1106 42-43 0.7346430836317097 1106 44-45 0.7908054600648526 1106 46-47 0.8096088756280224 1106 48-49 0.8546687096948986 1106 50-51 0.8487372970215574 1106 52-53 0.7408157289704889 1106 54-55 0.8821480386217502 1106 56-57 0.8231259201823917 1106 58-59 0.8181297362797721 1106 60-61 1.1492745029773417 1106 62-63 0.9575610411759321 1106 64-65 0.9965116892088481 1106 66-67 1.3840660464431132 1106 68-69 2.8105118764763155 1106 70-71 3.153269468213516 1106 72-73 2.0410377819049224 1106 74-75 1.6505110851269826 1106 76-77 1.1924690957715818 1106 78-79 1.1734986364582802 1106 80-81 1.2830009873252628 1106 82-83 1.378217700569305 1106 84-85 1.4178548493638807 1106 86-87 1.3480584728899672 1106 88-89 1.4776583414584188 1106 90-91 1.3841203566031268 1106 92-93 1.3491819108202456 1106 94-95 1.7774826679756437 1106 96-97 1.4924548436725154 1106 98-99 1.9769066963985935 1106 100-101 2.427399365897923 1107 1 0.07606709841064685 1107 2 0.17549255830783395 1107 3 0.14321173403580545 1107 4 0.05741322807165972 1107 5 0.184525818112391 1107 6 0.18472056338475795 1107 7 0.003037528547899626 1107 8 0.25150090260684266 1107 9 0.026158222016022137 1107 10-11 -0.0033586917412904427 1107 12-13 0.0762441593378611 1107 14-15 0.2702541503956368 1107 16-17 0.06301991738983048 1107 18-19 0.2266114318248853 1107 20-21 0.12716709269516002 1107 22-23 0.14909760628925284 1107 24-25 0.17873342686287685 1107 26-27 0.07959672070906976 1107 28-29 0.08015701946826681 1107 30-31 0.06283480827490706 1107 32-33 0.017486287877162 1107 34-35 0.2114218015462015 1107 36-37 0.37839813305064496 1107 38-39 0.4083843561485949 1107 40-41 0.3164689174002504 1107 42-43 0.21220925987473294 1107 44-45 0.3033520866644537 1107 46-47 0.23993057153195707 1107 48-49 0.2688297109196114 1107 50-51 0.3721334967418315 1107 52-53 0.1329467211609412 1107 54-55 0.3553960501290021 1107 56-57 0.4322104250451204 1107 58-59 0.6071527551927431 1107 60-61 0.5148060425283418 1107 62-63 0.6599610122899087 1107 64-65 0.6390918147451181 1107 66-67 0.983824126053559 1107 68-69 2.4109869482860695 1107 70-71 2.673098760697698 1107 72-73 1.6607942698369058 1107 74-75 0.8429151680770133 1107 76-77 0.7786850128510245 1107 78-79 0.8891253819621561 1107 80-81 0.8807444146511365 1107 82-83 0.8167951231997321 1107 84-85 0.7811315110835757 1107 86-87 0.5051932453167893 1107 88-89 0.46779177717008125 1107 90-91 0.22142502596876668 1107 92-93 0.26058363425432063 1107 94-95 0.7969508852515474 1107 96-97 0.4208079428031368 1107 98-99 0.6838333716033418 1107 100-101 0.9340652563757068 1108 1 -0.21862208082614387 1108 2 -0.1474350759629175 1108 3 -0.2583921502008515 1108 4 -0.28944310427061026 1108 5 -0.153259566901653 1108 6 -0.07758406551243269 1108 7 -0.10750364599726225 1108 8 -0.2166000766354017 1108 9 -0.34095533189685057 1108 10-11 -0.18712004576872943 1108 12-13 -0.17762371115122022 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-0.45833821177280143 2316 80-81 -0.5215671597221494 2316 82-83 -0.3368193760508298 2316 84-85 -0.5983249045322729 2316 86-87 -0.5215899397329196 2316 88-89 -0.5572972617595262 2316 90-91 -0.6568095967674417 2316 92-93 -0.4809449475858649 2316 94-95 -0.126004657163989 2316 96-97 -0.12700836885374756 2316 98-99 -0.2562100821445483 2316 100-101 -0.481255627550528 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 13.0 4 30.0 5 103.0 6 217.0 7 437.0 8 943.0 9 1552.0 10 2717.0 11 4064.0 12 5702.0 13 7475.0 14 8753.0 15 11422.0 16 14061.0 17 16896.0 18 14334.0 19 10831.0 20 10004.0 21 10369.0 22 11038.0 23 12293.0 24 14813.0 25 17647.0 26 21446.0 27 26159.0 28 31691.0 29 37934.0 30 45243.0 31 53664.0 32 64010.0 33 77190.0 34 95985.0 35 121643.0 36 148530.0 37 167181.0 38 101515.0 39 8258.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.619615050008804 20.352191908617538 14.686710440490758 21.3414826008829 2 15.735816130584976 22.209361855566648 39.37015574744999 22.684666266398384 3 18.956353912928883 27.620614454604585 30.705640790195254 22.71739084227128 4 12.332017759397909 18.178216340210483 37.437487565530716 32.052278334860894 5 12.267996184212091 39.835363375577934 33.70366087780126 14.19297956240871 6 29.311848837664073 37.84805886243949 17.97186791660361 14.868224383292835 7 27.00052543603539 35.165274289513555 20.67259213410352 17.161608140347536 8 26.718592443583645 34.90391662401396 19.769658364550583 18.60783256785182 9 27.60503194276998 16.266666439941304 20.33794549408842 35.7903561232003 10-11 24.705568771755008 27.909155680405657 26.719357641693435 20.6659179061459 12-13 26.206887463164215 25.684384687195518 27.65876585447972 20.44996199516055 14-15 22.974775669420815 26.300964319662363 27.147698539151786 23.57656147176504 16-17 20.709774673896963 29.903746252642065 27.726410137379037 21.660068936081935 18-19 20.61881571138768 27.54730199648689 31.559320708131334 20.274561583994096 20-21 21.961780905076324 26.543234543423296 31.39161478906889 20.103369762431495 22-23 21.38669201456257 26.778277896147312 31.12213752140429 20.712892567885827 24-25 20.778701260496522 27.112131944240748 31.67012919547325 20.439037599789486 26-27 20.89057270475346 27.256348920580415 31.520973469272366 20.33210490539376 28-29 20.41316481956188 27.265137119366756 31.810927888519043 20.51077017255232 30-31 20.545869145465627 27.35981723590563 31.65799580159519 20.436317817033554 32-33 20.24697771563468 27.485453456374398 31.291212727072537 20.976356100918387 34-35 20.830047440149272 27.318579371803825 31.29468371887577 20.55668946917113 36-37 20.99774379330389 27.00260685066397 31.23744128628463 20.762208069747512 38-39 20.710609053963722 27.1742733345446 31.387360737790264 20.727756873701413 40-41 20.631750687037066 27.257307189186474 31.60987529075136 20.5010668330251 42-43 20.61399103963396 27.32141398116689 31.07858874234862 20.98600623685053 44-45 20.540412883953778 27.66909710623794 30.790002787168813 21.000487222639467 46-47 20.69470831143799 27.6943402568723 30.110863141143607 21.500088290546103 48-49 20.67795239908687 27.767029123487074 29.979001474364566 21.576017003061487 50-51 20.852195291527618 27.911074385932366 29.77266934701847 21.464060975521544 52-53 20.980654492858434 28.037606219490147 29.64174120199517 21.339998085656248 54-55 20.37989845781099 27.925207916479504 29.933252344590088 21.761641281119417 56-57 20.697495815586432 28.38282621416033 29.24220440349994 21.677473566753303 58-59 20.626360773499687 27.776169260772356 29.49538341447487 22.10208655125309 60-61 20.50410500444491 27.91598590043318 29.678899414105263 21.901009681016646 62-63 20.768495640832484 27.780191507488038 29.53380629209738 21.917506559582094 64-65 20.47334777640225 27.569738256220493 29.723954861441765 22.23295910593549 66-67 20.2096615288721 27.912478956784454 29.600747294930297 22.277112219413148 68-69 20.259492795566185 27.753290613832444 29.71664609232671 22.270570498274658 70-71 20.100142206713592 27.793260801649804 29.585654364277946 22.520942627358657 72-73 20.182529390596287 27.720746215581432 29.162328080183464 22.934396313638814 74-75 20.194598575591492 27.868153979673536 28.841316946413105 23.095930498321863 76-77 20.21633477672749 27.68748354866597 29.118914388427108 22.977267286179433 78-79 20.217751278045252 27.80410037305105 28.92852511983335 23.04962322907035 80-81 19.986957468082355 28.098366537658396 28.819361783170883 23.095314211088365 82-83 20.1600369335621 28.012925046294484 28.350802522773144 23.47623549737027 84-85 19.94220181497184 27.46242756628235 28.584594887691868 24.01077573105394 86-87 20.03235131573354 27.460783063384774 28.640649577009736 23.866216043871955 88-89 20.305246547250213 27.240843963937 28.575054159113865 23.87885532969892 90-91 19.819624880968576 27.43185450959053 28.7276816079445 24.020839001496395 92-93 19.90507868164458 27.24664408416742 28.72283893304493 24.125438301143067 94-95 19.508222037775468 26.96776488115876 28.939199080394438 24.584814000671326 96-97 19.86281392592139 26.671904788629874 28.67174492386866 24.793536361580077 98-99 19.75155997816286 26.722481202879646 28.50763364620767 25.01832517274983 100-101 19.598523202086973 26.660207234677202 28.818774244127415 24.92249531910841 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2156.0 1 1419.0 2 898.0 3 1183.5 4 1085.0 5 1058.0 6 1731.5 7 3331.5 8 4818.0 9 5104.0 10 4165.5 11 3132.0 12 2800.0 13 2691.0 14 2780.5 15 2805.5 16 2739.0 17 2809.5 18 2909.0 19 3125.5 20 3446.5 21 3766.5 22 4240.5 23 4833.5 24 5383.0 25 6165.0 26 7453.5 27 9057.5 28 10594.0 29 12340.0 30 14546.5 31 16749.0 32 19781.0 33 22925.0 34 25652.5 35 28022.5 36 30732.5 37 33528.0 38 34711.0 39 35359.0 40 36126.0 41 37710.5 42 39218.0 43 40124.0 44 40982.5 45 41886.0 46 42504.5 47 43101.5 48 47487.0 49 47053.0 50 41760.0 51 38883.0 52 35708.5 53 34345.5 54 33020.0 55 30191.0 56 27170.5 57 24247.0 58 21807.5 59 19245.0 60 16340.0 61 13488.5 62 10884.5 63 8206.0 64 5922.5 65 4571.0 66 3422.5 67 2581.0 68 1886.5 69 1193.0 70 842.5 71 690.0 72 596.5 73 579.5 74 475.0 75 355.0 76 250.0 77 137.5 78 84.0 79 62.5 80 36.0 81 19.0 82 9.0 83 5.5 84 2.5 85 2.0 86 1.0 87 0.0 88 0.0 89 0.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02321100933031562 2 0.024486339513299996 3 8.502201219895831E-5 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 3.4008804879583326E-4 18-19 0.0 20-21 0.0 22-23 0.0 24-25 2.1255503049739577E-4 26-27 1.2753301829843746E-4 28-29 8.502201219895831E-5 30-31 4.2511006099479155E-4 32-33 0.0023381053354713534 34-35 0.004463655640445312 36-37 0.10075108445576558 38-39 0.09263148229076508 40-41 0.10143126055335726 42-43 0.1032167228095354 44-45 0.09679756088851403 46-47 0.09084602003458694 48-49 0.09212135021757133 50-51 0.09110108607118382 52-53 0.07044073710683696 54-55 0.05900527646607707 56-57 0.031798232562410406 58-59 0.011435460640759893 60-61 0.008629734238194268 62-63 0.0020405282927749993 64-65 0.004591188658743748 66-67 0.15278455592152806 68-69 0.4084457466037957 70-71 0.39382196050557494 72-73 0.17926891272150358 74-75 0.026314312775577598 76-77 0.02971519326353593 78-79 0.0045486776526442695 80-81 0.0011903081707854164 82-83 0.0 84-85 3.8259905489531243E-4 86-87 0.001360352195183333 88-89 1.7004402439791663E-4 90-91 5.101320731937498E-4 92-93 0.0028907484147645823 94-95 0.07477685972898383 96-97 0.014623786098220829 98-99 0.015984138293404164 100-101 0.009182377317487497 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1176166.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 50.24158635837077 #Duplication Level Percentage of deduplicated Percentage of total 1 74.84525293425077 37.60344238810263 2 13.424289132916783 13.489151635423536 3 4.795358316351875 7.227792269109726 4 2.2133773304337874 4.448143531625972 5 1.2531766412684073 3.148079122228986 6 0.7373448751681692 2.2227225732998206 7 0.5063948723869156 1.7809457198724357 8 0.34271658086816326 1.3774899755306707 9 0.26099560850263304 1.1801550063366508 >10 1.4312026618954519 13.546409548850788 >50 0.12284455444134075 4.275176153071175 >100 0.06366883621071448 5.712486350811458 >500 0.0015199448872592388 0.5729878654900378 >1k 0.0013510621220082122 1.2231886670967547 >5k 3.3776553050205306E-4 1.3054925660343137 >10k+ 1.6888276525102653E-4 0.8863366271151983 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10446 0.8881399394303185 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8847 0.7521897419241841 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6539 0.5559589377689884 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3387 0.2879695553178718 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2051 0.1743801470200635 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1983 0.16859865019053433 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1938 0.16477265964158122 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 1744 0.1482783892749833 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 1187 0.1009211284801635 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 1.2753301829843746E-4 0.0 12-13 0.0 0.0 0.0 1.7004402439791663E-4 0.0 14-15 0.0 0.0 0.0 1.7004402439791663E-4 0.0 16-17 0.0 0.0 0.0 2.550660365968749E-4 0.0 18-19 0.0 0.0 0.0 2.550660365968749E-4 0.0 20-21 0.0 0.0 0.0 5.951540853927082E-4 0.0 22-23 0.0 0.0 0.0 0.0016154182317802078 0.0 24-25 0.0 0.0 0.0 0.0032308364635604157 0.0 26-27 0.0 0.0 0.0 0.0077370031101052054 0.0 28-29 0.0 0.0 0.0 0.01708942445199062 0.0 30-31 0.0 0.0 0.0 0.04744228280701873 0.0 32-33 0.0 0.0 0.0 0.10432200896812185 0.0 34-35 0.0 0.0 0.0 0.1957631830881015 0.0 36-37 0.0 0.0 0.0 0.3434889292837916 0.0 38-39 0.0 0.0 0.0 0.5614003465497217 0.0 40-41 0.0 0.0 0.0 0.8088144020486904 0.0 42-43 0.0 0.0 0.0 1.0409244953518466 0.0 44-45 0.0 0.0 0.0 1.3120171812482253 0.0 46-47 0.0 0.0 0.0 1.6368862898604448 0.0 48-49 0.0 0.0 0.0 1.9889199313702317 0.0 50-51 0.0 0.0 0.0 2.3654824233994183 0.0 52-53 0.0 0.0 0.0 2.71632575673842 0.0 54-55 0.0 0.0 0.0 3.070740014589777 0.0 56-57 0.0 0.0 0.0 3.4646469971075513 0.0 58-59 0.0 0.0 0.0 3.8942632247488875 0.0 60-61 0.0 0.0 0.0 4.351511606354885 0.0 62-63 0.0 0.0 0.0 4.77313576484952 0.0 64-65 0.0 0.0 0.0 5.1914015538622955 0.0 66-67 0.0 0.0 0.0 5.622378133698815 0.0 68-69 0.0 0.0 0.0 6.107003603232878 0.0 70-71 0.0 0.0 0.0 6.683962978015009 0.0 72-73 0.0 0.0 0.0 7.2470637648087095 0.0 74-75 0.0 0.0 0.0 7.790779532821047 0.0 76-77 0.0 0.0 0.0 8.37245762928022 0.0 78-79 0.0 0.0 0.0 9.011015451900498 0.0 80-81 0.0 0.0 0.0 9.694549918974023 0.0 82-83 0.0 0.0 0.0 10.391645396993281 0.0 84-85 0.0 0.0 0.0 11.045932291870365 0.0 86-87 0.0 0.0 0.0 11.711144515315015 0.0 88-89 0.0 0.0 0.0 12.396421933638619 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 7035 0.0 34.592625 1 GTACACG 170 1.09139364E-10 30.695452 1 GTATCAA 10510 0.0 30.331705 1 GTACATG 9665 0.0 26.946402 1 TACATGG 9695 0.0 26.61497 2 TAAGGTG 385 0.0 25.868908 5 ACATGGG 9775 0.0 25.471941 3 TATCAAC 13150 0.0 24.239208 2 ATCAACG 13150 0.0 24.20005 3 TCAACGC 13265 0.0 24.026001 4 CAACGCA 13490 0.0 23.660429 5 CATGGGG 2600 0.0 23.348345 4 AACGCAG 13760 0.0 23.161695 6 GAGTACT 7685 0.0 22.370909 12-13 CATGGGA 6925 0.0 22.257843 4 TACAGCG 110 0.002903438 21.55742 7 AGTACTT 7845 0.0 21.370564 12-13 GTACTTT 8245 0.0 20.93776 14-15 ACGCAGA 15240 0.0 20.881275 7 GCACCGT 250 9.660653E-9 20.867586 6 >>END_MODULE