FastQCFastQC Report
Fri 27 May 2016
SRR939769_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939769_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2802032
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50800.18129700160454984No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36000.1284781901134605No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA32900.050.901271
GTATCAA63300.043.6932831
TCAACGC76150.036.3851244
ATCAACG76450.036.1182253
TATCAAC77200.036.0720332
CAACGCA77950.035.3014685
AACGCAG80800.034.349896
ACGCAGA96850.028.315027
CGCAGAG97850.027.8321978
TACATGG78800.025.8876172
GTACATG81300.025.208271
ACATGGG79400.023.9012373
GCAGAGT109300.023.744539
TGGTATC18700.023.086052
GTGGTAT19800.022.2826881
CAGAGTA101800.020.22623610-11
AGAGTAC94650.020.10003710-11
GAGTACT64350.020.05347312-13
CATGGGG48350.019.723344
CCCGTAC1507.7864114E-418.9775833
Produced by FastQC (version 0.11.5)