FastQCFastQC Report
Fri 27 May 2016
SRR939769_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939769_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2802032
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58260.20792053766695026No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46540.16609374910779034No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32180.11484522660697666No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA33650.052.711921
GTATCAA63750.040.847451
ATCAACG78800.032.1992533
TCAACGC79200.032.0956044
TATCAAC82100.031.1955722
CAACGCA81850.031.1140885
AACGCAG85700.029.6411676
ACGCAGA99250.025.3570167
CGCAGAG100100.024.9533678
TACATGG79400.022.0388872
GTACATG83750.021.5171431
GCAGAGT115950.021.0139269
ACATGGG80400.020.5306053
AGAGTAC100650.020.20479210-11
TGGTATC17550.019.8879872
GTGGTAT20450.019.1465641
CAGAGTA107400.018.75941310-11
GAGTACT64400.018.51509512-13
TAAGGTG15900.018.093175
AGTACTT70200.017.48887412-13
Produced by FastQC (version 0.11.5)