FastQCFastQC Report
Fri 27 May 2016
SRR939768_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939768_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2900386
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51850.1787693086368504No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37970.13091360942991725No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN29180.10060729847682344No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA37200.051.7166821
GTATCAA70100.041.2674261
ATCAACG84400.033.2739753
TATCAAC86850.032.8216672
TCAACGC85800.032.731614
CAACGCA87550.031.9156075
AACGCAG92700.030.2947546
ACGCAGA109900.024.9113967
CGCAGAG110100.024.7375438
GTACATG89200.023.6245461
TACATGG87500.023.6001222
ACATGGG90100.022.3455893
GCAGAGT118450.022.0008779
AGAGTAC102850.020.07736810-11
GAGTACT65800.020.01623712-13
AGTACTT70650.019.64084612-13
CAGAGTA111400.019.42312810-11
GTACTTT69400.018.8798514-15
GTGGTAT22000.018.6014541
ATACCGT3106.3064363E-918.2092636
Produced by FastQC (version 0.11.5)