FastQCFastQC Report
Fri 27 May 2016
SRR939768_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939768_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2900386
Sequences flagged as poor quality0
Sequence length101
%GC46

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58980.2033522434600084No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48510.1672536000380639No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32670.1126401796174716No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA35600.053.3253061
GTATCAA63550.040.999651
ATCAACG81050.031.8379083
TCAACGC80950.031.7600444
TATCAAC83950.030.8574812
CAACGCA83700.030.7165555
AACGCAG88850.028.9895236
ACGCAGA102900.024.846897
CGCAGAG105350.024.2240318
GTACATG86000.021.6327111
TACATGG84550.021.1551552
GCAGAGT121500.020.5746759
TGGTATC18500.020.5166652
ACATGGG86950.020.130543
GTGGTAT21750.020.074831
AGAGTAC106700.019.42744410-11
GAGTACT71900.018.86843112-13
CAGAGTA113150.018.86499210-11
GTACTTT74900.017.9543614-15
AGTACTT78400.017.3645912-13
Produced by FastQC (version 0.11.5)