FastQCFastQC Report
Fri 27 May 2016
SRR939767_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939767_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1548296
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT124360.8032055885954623No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT81790.5282581625218951No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63900.4127117812097945No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA24480.1581093020972734No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA21380.13808729080227553No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17550.11335041878297172No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA17400.11238161178482667No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16240.10488950433250491No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA139150.036.2778431
GGTATCA85800.032.8459781
TATCAAC175300.029.8792842
ATCAACG178700.029.1533813
TCAACGC180050.028.908444
CAACGCA182150.028.5733075
AACGCAG184550.028.1760146
TACATGG132650.027.468632
GTACATG131900.027.2291531
ACATGGG135550.025.6225723
ACGCAGA206600.025.1458827
CGCAGAG208350.024.7988458
GCAGAGT203050.024.2310859
ATGGGAG29400.024.0448465
ATGGGAT32500.023.9410695
AGTACTT92300.023.90122812-13
GAGTACT90450.023.65576212-13
CATGGGA92400.023.5695824
CATGGGG43150.023.0913684
GTACTTT100050.021.50588414-15
Produced by FastQC (version 0.11.5)