##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939767_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1548296 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.077674424011946 31.0 30.0 33.0 16.0 34.0 2 29.844219064054936 31.0 30.0 33.0 25.0 34.0 3 30.086021665107964 31.0 30.0 34.0 26.0 34.0 4 33.89303208172081 35.0 35.0 37.0 28.0 37.0 5 33.86521957041806 35.0 35.0 37.0 28.0 37.0 6 33.93709019463979 35.0 35.0 37.0 29.0 37.0 7 33.858565158083465 35.0 35.0 37.0 28.0 37.0 8 33.74165534238931 35.0 35.0 37.0 28.0 37.0 9 34.99569655931424 37.0 35.0 39.0 28.0 39.0 10-11 34.82596932369521 37.0 34.0 39.0 27.0 39.0 12-13 34.6997738158595 37.0 34.0 39.0 27.0 39.0 14-15 35.276814317158994 38.0 33.5 40.0 27.0 41.0 16-17 35.15721412443099 38.0 33.0 40.0 27.0 41.0 18-19 35.09592803959966 38.0 33.5 40.0 26.5 41.0 20-21 35.298874698378086 38.0 34.0 40.0 27.0 41.0 22-23 35.186012235386514 38.0 34.0 40.0 26.5 41.0 24-25 34.95113337501356 38.0 34.0 40.0 25.0 41.0 26-27 34.78317711858715 38.0 33.5 40.0 25.0 41.0 28-29 34.78958351633021 38.0 34.0 40.0 25.0 41.0 30-31 34.503021709027216 38.0 33.0 40.0 24.0 41.0 32-33 34.07946219585919 38.0 33.0 40.0 23.5 41.0 34-35 33.83696560605982 38.0 33.0 40.0 22.5 41.0 36-37 33.56416085813049 37.0 32.5 40.0 20.5 41.0 38-39 33.27060297255822 37.0 32.0 40.0 18.5 41.0 40-41 32.94874042172815 37.0 31.0 40.0 17.0 41.0 42-43 32.444715674522186 37.0 31.0 40.0 14.5 41.0 44-45 31.94616630153407 36.5 30.5 39.5 9.0 41.0 46-47 31.382159806651956 36.0 30.0 39.0 7.0 41.0 48-49 30.89920758046265 36.0 29.0 39.0 2.0 40.0 50-51 30.188708748198017 35.0 27.5 38.5 2.0 40.0 52-53 30.25830784294476 35.0 28.0 38.5 2.0 40.0 54-55 30.318222420002378 35.0 28.0 39.0 2.0 40.0 56-57 30.00225118452802 35.0 28.0 39.0 2.0 40.0 58-59 29.579750900344635 35.0 27.0 38.5 2.0 40.0 60-61 29.146405790624016 34.0 26.0 38.0 2.0 40.0 62-63 28.672572298836915 34.0 26.0 38.0 2.0 40.0 64-65 28.09851733777004 34.0 25.5 37.5 2.0 40.0 66-67 27.49308207216191 33.0 23.0 37.0 2.0 39.0 68-69 26.917345262146256 33.0 22.5 36.0 2.0 39.0 70-71 26.330843391702878 32.0 20.5 35.5 2.0 38.5 72-73 25.680139973235093 32.0 19.0 35.0 2.0 38.0 74-75 25.29762655202881 31.5 18.0 35.0 2.0 37.0 76-77 24.701721440861437 31.0 12.5 35.0 2.0 37.0 78-79 24.01104956674951 31.0 7.0 34.5 2.0 36.0 80-81 23.253044314523837 30.0 2.0 34.0 2.0 35.5 82-83 22.473915517446276 29.5 2.0 34.0 2.0 35.0 84-85 21.64748665629828 29.0 2.0 34.0 2.0 35.0 86-87 20.63278016606644 28.0 2.0 34.0 2.0 35.0 88-89 19.578112970646437 26.5 2.0 33.0 2.0 35.0 90-91 18.14790227450048 22.5 2.0 32.5 2.0 34.5 92-93 16.612316378780285 15.5 2.0 31.5 2.0 34.0 94-95 16.09532673338948 13.5 2.0 31.0 2.0 34.0 96-97 15.792661416163318 4.5 2.0 31.0 2.0 34.0 98-99 14.947207446121414 2.0 2.0 31.0 2.0 34.0 100-101 13.418372843435622 2.0 2.0 30.0 2.0 33.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.8250953425944658 1101 2 1.0625623069432208 1101 3 0.9631959733383297 1101 4 0.8940434452537573 1101 5 1.088852488401706 1101 6 1.098225442750909 1101 7 0.9626724359567689 1101 8 1.1185818882621348 1101 9 1.1367624214981475 1101 10-11 1.2058337627842448 1101 12-13 1.2039772112163334 1101 14-15 1.2281779016561103 1101 16-17 1.2319954920779068 1101 18-19 1.2268319966874017 1101 20-21 1.3168153306016492 1101 22-23 1.4658396202840862 1101 24-25 1.5793406244141224 1101 26-27 1.4998483564519276 1101 28-29 1.426430941190894 1101 30-31 1.5397230924883871 1101 32-33 1.6696800737001851 1101 34-35 1.644138451062453 1101 36-37 1.5487859065056924 1101 38-39 1.5734679571712817 1101 40-41 1.4934992392125537 1101 42-43 1.4560315775443158 1101 44-45 1.51597426840301 1101 46-47 1.5616718837422177 1101 48-49 1.5129815317523736 1101 50-51 1.3368174080990443 1101 52-53 1.3561092236539949 1101 54-55 1.335806561923988 1101 56-57 1.3039247889860768 1101 58-59 1.3310941395607045 1101 60-61 0.9798886907048114 1101 62-63 0.8248577060748765 1101 64-65 1.0084605726054576 1101 66-67 1.2054803368370557 1101 68-69 1.2101288190849218 1101 70-71 1.0708710785798559 1101 72-73 1.0433129321229409 1101 74-75 0.8535807377177349 1101 76-77 0.5431475011880131 1101 78-79 0.45738558026692644 1101 80-81 0.1272510478589517 1101 82-83 0.4110486414669374 1101 84-85 0.22395238833096798 1101 86-87 -0.33697459818016284 1101 88-89 -4.141787090853876 1101 90-91 -7.435734853853118 1101 92-93 -9.431986708857352 1101 94-95 -7.467806563323569 1101 96-97 -4.601090207883219 1101 98-99 -3.2343839264776104 1101 100-101 -2.260676735875583 1102 1 1.7890636776302173 1102 2 1.1154947209711388 1102 3 1.0641067666648532 1102 4 1.0150214840688747 1102 5 1.0791444529914216 1102 6 1.1413817246712412 1102 7 1.150368213961535 1102 8 1.2854187966720758 1102 9 1.2778204739326071 1102 10-11 1.2985346480413043 1102 12-13 1.1602740276957348 1102 14-15 1.6352237818059265 1102 16-17 1.4885497991940042 1102 18-19 1.5613165898096355 1102 20-21 1.621694884568278 1102 22-23 1.3906417142575265 1102 24-25 1.638308108241155 1102 26-27 1.6526232968673114 1102 28-29 1.4610900320999818 1102 30-31 1.529244713189783 1102 32-33 1.742234976662381 1102 34-35 1.8195725675078052 1102 36-37 1.8113156988107662 1102 38-39 1.5480487368784637 1102 40-41 1.5847296614120765 1102 42-43 1.6299143661040674 1102 44-45 1.6679767369082903 1102 46-47 1.9304623161562482 1102 48-49 1.704228978126224 1102 50-51 1.8033436253275106 1102 52-53 1.9046919611412285 1102 54-55 1.7554482030543994 1102 56-57 1.8569040194933955 1102 58-59 1.5699071290057205 1102 60-61 1.5981300934967742 1102 62-63 1.3570270114885652 1102 64-65 1.524450528342605 1102 66-67 1.5843958969323921 1102 68-69 1.3279339774097423 1102 70-71 1.3403467340105664 1102 72-73 1.3409274196952943 1102 74-75 1.1833964510306387 1102 76-77 0.9877487099043911 1102 78-79 0.8384951308287256 1102 80-81 0.6624166079542881 1102 82-83 0.8845441300607426 1102 84-85 0.7450347002138393 1102 86-87 0.541202538353712 1102 88-89 0.26708676001435805 1102 90-91 -5.0124521674384095 1102 92-93 -9.704437338751802 1102 94-95 -7.601537836731808 1102 96-97 -4.2673631905185605 1102 98-99 -2.6866119652927285 1102 100-101 -1.893124812143883 1103 1 2.012539455858253 1103 2 1.309037007106447 1103 3 0.9400606387275836 1103 4 1.0054975649045943 1103 5 0.9357955726398686 1103 6 0.8328698966576624 1103 7 0.911849215461416 1103 8 0.933791951423018 1103 9 1.0540536002647372 1103 10-11 1.0552660245115888 1103 12-13 1.0728491862358496 1103 14-15 1.2576080343649352 1103 16-17 1.1312441259576858 1103 18-19 1.083055827501397 1103 20-21 1.0045158131791823 1103 22-23 1.0964256326323891 1103 24-25 1.1189181916556166 1103 26-27 1.1545951806659573 1103 28-29 1.152907947790844 1103 30-31 1.2238823075902232 1103 32-33 1.1814429010367817 1103 34-35 1.1775640418650966 1103 36-37 1.3461583428125508 1103 38-39 1.0510822144986562 1103 40-41 0.9434176268358598 1103 42-43 1.165308775755939 1103 44-45 1.164749195661546 1103 46-47 0.8967643186297316 1103 48-49 0.9452601138260412 1103 50-51 1.0288060888172375 1103 52-53 0.8417117358958528 1103 54-55 0.8415433797509735 1103 56-57 0.911572400936965 1103 58-59 1.1041026201511563 1103 60-61 1.2370484025776385 1103 62-63 1.1617110166986784 1103 64-65 0.7023706925401676 1103 66-67 0.7930850135810772 1103 68-69 0.6746327577691886 1103 70-71 0.7321513283846954 1103 72-73 0.878216416616592 1103 74-75 0.6885268025818334 1103 76-77 0.485249729560735 1103 78-79 0.20527785192969006 1103 80-81 0.32068125153505633 1103 82-83 -0.14001231943008818 1103 84-85 -0.4395979274734465 1103 86-87 -0.2032970735190318 1103 88-89 -0.8653633097169795 1103 90-91 -6.434492039290632 1103 92-93 -9.140475283124616 1103 94-95 -8.041791731161196 1103 96-97 -4.710787887253028 1103 98-99 -3.1920909735289182 1103 100-101 -2.4904954144647533 1104 1 2.015852559948371 1104 2 1.2970071396583087 1104 3 1.1231694514667439 1104 4 0.8342482729511929 1104 5 0.6582766233048432 1104 6 0.8500600215369971 1104 7 0.9083886160904484 1104 8 0.9736949646200372 1104 9 0.9526800244943985 1104 10-11 1.0286130040436703 1104 12-13 0.9509858845851511 1104 14-15 1.0950663305936175 1104 16-17 1.0162331226712382 1104 18-19 1.1674113562200432 1104 20-21 1.0829419277380055 1104 22-23 0.9646548721522663 1104 24-25 1.0405109467982214 1104 26-27 1.095963036666248 1104 28-29 1.1653515637380636 1104 30-31 1.0921143169745235 1104 32-33 1.3136978684423966 1104 34-35 1.1552467248111782 1104 36-37 0.9941217307445314 1104 38-39 1.0815533199654013 1104 40-41 1.02439184355282 1104 42-43 1.103654017198103 1104 44-45 0.8934904861690818 1104 46-47 0.8054623161562411 1104 48-49 0.8236294807873747 1104 50-51 0.920360183577067 1104 52-53 0.7894864614369119 1104 54-55 0.8214594390094518 1104 56-57 1.1068670591149221 1104 58-59 0.9990356032812961 1104 60-61 0.9644444045553193 1104 62-63 0.9063506365624825 1104 64-65 0.7235930475620016 1104 66-67 0.8891342174527921 1104 68-69 0.9181727414546828 1104 70-71 0.8887648298118691 1104 72-73 0.8613110684238592 1104 74-75 0.6564708153121259 1104 76-77 0.6749345466873571 1104 78-79 0.6244760592734337 1104 80-81 0.46580587466037926 1104 82-83 0.31921666110746116 1104 84-85 0.20506205089391116 1104 86-87 0.3136929286553105 1104 88-89 0.09995192855368273 1104 90-91 -2.478393178674363 1104 92-93 -6.973361052530631 1104 94-95 -5.022202384336776 1104 96-97 -2.637835544155463 1104 98-99 -1.8057537453045551 1104 100-101 -1.3316966631196383 1105 1 1.9133751999919717 1105 2 1.1747726456241487 1105 3 0.8198693244969739 1105 4 0.7217506645050946 1105 5 0.7203030231610654 1105 6 0.7479580630717706 1105 7 0.7407584430868894 1105 8 0.7274834034136504 1105 9 0.7780614411059901 1105 10-11 0.6966537271336151 1105 12-13 0.7265262308241773 1105 14-15 0.8344279016561131 1105 16-17 0.7417994136465325 1105 18-19 0.9078480394681563 1105 20-21 0.7582647512790146 1105 22-23 0.6504318662733937 1105 24-25 0.645767763451552 1105 26-27 0.42763913185299174 1105 28-29 0.6173400320999818 1105 30-31 0.7246105702067496 1105 32-33 1.0734512412581125 1105 34-35 0.9844905009733296 1105 36-37 1.1734962451866195 1105 38-39 1.0608509785616533 1105 40-41 0.8609847561465998 1105 42-43 0.8346022103428865 1105 44-45 0.6408851418432953 1105 46-47 0.8278929176103098 1105 48-49 0.5356586707898074 1105 50-51 0.6070379963343377 1105 52-53 0.42599001688037674 1105 54-55 0.5375278186084813 1105 56-57 0.41687042178465106 1105 58-59 0.4335506903628463 1105 60-61 0.6401995195818166 1105 62-63 0.3076562800499225 1105 64-65 0.5288705547801449 1105 66-67 0.33797253826308093 1105 68-69 0.5292241845038141 1105 70-71 0.7486897060308344 1105 72-73 0.7359655613564549 1105 74-75 0.38757137942896236 1105 76-77 0.34206684165147294 1105 78-79 0.2723978351688885 1105 80-81 0.24411824928497694 1105 82-83 0.1532500674918964 1105 84-85 0.19549873414201713 1105 86-87 0.45626849451145546 1105 88-89 0.22047338151688933 1105 90-91 -2.561492429610695 1105 92-93 -7.14363443613008 1105 94-95 -4.88220607312749 1105 96-97 -2.4404395839973 1105 98-99 -1.507311733964242 1105 100-101 -0.9970849355190765 1106 1 1.7742722164005116 1106 2 1.2009214858771848 1106 3 0.7882512229918461 1106 4 0.8099609640760974 1106 5 0.8107098131947552 1106 6 0.8607970800135263 1106 7 0.7329911983645232 1106 8 -0.16462877211169058 1106 9 -0.6974567791611008 1106 10-11 -0.4246228428268708 1106 12-13 -0.2572197397519389 1106 14-15 0.5060003161596143 1106 16-17 0.7184242106605154 1106 18-19 0.9363630301368531 1106 20-21 0.8158540802739012 1106 22-23 0.8179339567263852 1106 24-25 0.770946891035841 1106 26-27 0.9488318564706546 1106 28-29 0.9184061631259439 1106 30-31 0.9514046484684542 1106 32-33 0.9447812086591654 1106 34-35 1.10712586537867 1106 36-37 0.8555216820610028 1106 38-39 0.8634467001011998 1106 40-41 0.7302992541955078 1106 42-43 0.9766322068285049 1106 44-45 0.9382581392499212 1106 46-47 1.0057859227728194 1106 48-49 0.8601708226839406 1106 50-51 0.8305081785976043 1106 52-53 0.8484679156418586 1106 54-55 1.2769183909684543 1106 56-57 1.18363646070949 1106 58-59 1.0985347695940462 1106 60-61 0.9007725915176366 1106 62-63 0.9469107457422545 1106 64-65 0.9646527477562259 1106 66-67 0.8245409990837516 1106 68-69 0.7722060187702766 1106 70-71 0.8744660517978176 1106 72-73 1.0343887084273966 1106 74-75 0.8515421191463588 1106 76-77 0.6290049945393257 1106 78-79 0.5997836025166166 1106 80-81 0.43921962110688284 1106 82-83 0.3076194172095299 1106 84-85 0.3321719448354443 1106 86-87 0.5339678298079988 1106 88-89 0.2836160443298539 1106 90-91 -2.7420909050948303 1106 92-93 -7.529804347543347 1106 94-95 -5.121926876467368 1106 96-97 -2.3556974671258306 1106 98-99 -1.4265066368945103 1106 100-101 -0.8585575839109421 1107 1 1.7392458916162603 1107 2 0.9902368141241666 1107 3 0.8149136405066386 1107 4 0.708644080669842 1107 5 0.5195619434673802 1107 6 0.6416943129365222 1107 7 0.4713545978578111 1107 8 0.5401558436343734 1107 9 0.47993634342743263 1107 10-11 0.5256050501520946 1107 12-13 0.4150082760027587 1107 14-15 0.44976486262075355 1107 16-17 0.5617906075489856 1107 18-19 0.698166742056479 1107 20-21 0.6629761261723175 1107 22-23 0.6046675841656963 1107 24-25 0.5621442120996818 1107 26-27 0.5991504670737768 1107 28-29 0.4772036127253685 1107 30-31 0.5687225443955626 1107 32-33 0.6176971853439 1107 34-35 0.8243750599471298 1107 36-37 0.515158553886927 1107 38-39 0.5310314058827998 1107 40-41 0.6113373381665284 1107 42-43 0.7498515987248524 1107 44-45 0.6541175807416089 1107 46-47 0.6014687642579801 1107 48-49 0.7131160002881316 1107 50-51 0.6406315303144545 1107 52-53 0.6569425105387836 1107 54-55 0.669253926433587 1107 56-57 0.6973130709473203 1107 58-59 0.46849613313222704 1107 60-61 0.18879403786123206 1107 62-63 0.3615808541714145 1107 64-65 0.6944830444111894 1107 66-67 0.6557560089474919 1107 68-69 0.3912126494176782 1107 70-71 0.3832918838118218 1107 72-73 0.3349122484003715 1107 74-75 0.5060002423904564 1107 76-77 0.5070079790506377 1107 78-79 0.5368153779370672 1107 80-81 0.3986104109565112 1107 82-83 -0.03430929101545743 1107 84-85 -0.05970678154065112 1107 86-87 0.1223680620208647 1107 88-89 -0.2522136278362055 1107 90-91 -3.2090884852003114 1107 92-93 -4.308183920086751 1107 94-95 -2.8865578406585364 1107 96-97 -1.5565553436058632 1107 98-99 -0.987856419649674 1107 100-101 -0.5002978086511636 1108 1 1.56985548120511 1108 2 0.9371092955908651 1108 3 -0.557803178393403 1108 4 -0.20575892628774284 1108 5 0.2242988218974702 1108 6 0.49117443836518504 1108 7 0.44635139348920916 1108 8 0.44396270657028936 1108 9 0.6746520383335621 1108 10-11 0.5760929358129232 1108 12-13 0.5305952378194689 1108 14-15 0.4658243181738797 1108 16-17 0.5458107591019257 1108 18-19 0.5362216044986852 1108 20-21 0.4914631639079161 1108 22-23 0.19668116557619442 1108 24-25 0.294267213112299 1108 26-27 0.4693041562606126 1108 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2311 78-79 1.0539321268134714 2311 80-81 1.2169521125600156 2311 82-83 1.3934947239130224 2311 84-85 1.5107698876484683 2311 86-87 1.612438451232883 2311 88-89 1.900613264046477 2311 90-91 2.3031096724914057 2311 92-93 3.1999372605666174 2311 94-95 2.6903015447845373 2311 96-97 1.9680880612950542 2311 98-99 2.119028576934893 2311 100-101 1.8668348666360206 2312 1 1.1714803334425454 2312 2 0.4388639136139538 2312 3 -0.4445729914776173 2312 4 -0.7842664998347146 2312 5 -0.3810631105407367 2312 6 -0.21652440471705603 2312 7 -0.052540092007440364 2312 8 -0.06463450384687519 2312 9 -0.28008728360659063 2312 10-11 -0.19757278592431504 2312 12-13 -0.026310565883527204 2312 14-15 0.214658479244072 2312 16-17 -0.2541368040376142 2312 18-19 -0.47871000046533396 2312 20-21 0.3228835632638436 2312 22-23 0.2721468197959851 2312 24-25 0.1999044844914053 2312 26-27 0.3817911060960171 2312 28-29 0.33694802477844377 2312 30-31 0.15613808130270002 2312 32-33 0.4061809888923733 2312 34-35 0.24961435100144058 2312 36-37 0.09310663052441015 2312 38-39 0.10444467061444129 2312 40-41 0.011823420623976233 2312 42-43 0.12447270018007117 2312 44-45 0.1313825458079343 2312 46-47 0.20927458864357718 2312 48-49 0.20312033590124656 2312 50-51 0.08505057873154342 2312 52-53 -0.059235498737706394 2312 54-55 0.1766876886272648 2312 56-57 0.007588099948044658 2312 58-59 0.14070619976001808 2312 60-61 0.04239377510586806 2312 62-63 0.16845136074235612 2312 64-65 0.07353327954058031 2312 66-67 0.20610317596660366 2312 68-69 0.3054155793045652 2312 70-71 0.1289501918603122 2312 72-73 0.3941874491048445 2312 74-75 0.21240217355259006 2312 76-77 0.2846336716171365 2312 78-79 0.34997252352501107 2312 80-81 0.26984307551809295 2312 82-83 0.48477245680149395 2312 84-85 0.7596728479608217 2312 86-87 1.2745364858141386 2312 88-89 1.6080069909032915 2312 90-91 2.346441778239619 2312 92-93 3.323212091223997 2312 94-95 3.01601487251129 2312 96-97 2.1145900849782837 2312 98-99 1.8632670802338023 2312 100-101 1.9218786840959456 2313 1 -7.526907575747035 2313 2 -2.854554500134217 2313 3 -1.5650054839969876 2313 4 -0.7808780899040002 2313 5 -0.5136368201899657 2313 6 -0.06911804053745385 2313 7 -0.20196974600825257 2313 8 -0.030182405187197503 2313 9 0.1454743132809071 2313 10-11 -0.07189415234524432 2313 12-13 -0.30065129675863034 2313 14-15 -0.23321418394856863 2313 16-17 -0.7171388709344058 2313 18-19 -0.9335730837964178 2313 20-21 -0.5516085355584366 2313 22-23 -0.9960312844017807 2313 24-25 -0.40667909733394936 2313 26-27 -0.5293352879876281 2313 28-29 -0.7620133900684039 2313 30-31 -0.07925736930702953 2313 32-33 -0.4266320787143343 2313 34-35 -0.2288242161915477 2313 36-37 -0.22067291551218204 2313 38-39 -0.39337146980593474 2313 40-41 -0.49678396839349404 2313 42-43 -0.5928134750408915 2313 44-45 -0.4551536522327808 2313 46-47 -1.3325201119261578 2313 48-49 -1.0017278730160797 2313 50-51 -0.9868626847739606 2313 52-53 -1.248744583955716 2313 54-55 -0.9579660368682923 2313 56-57 -0.7164652868173853 2313 58-59 -0.542781427129821 2313 60-61 -0.6186711811818348 2313 62-63 -0.6309073949658135 2313 64-65 -0.9061175985671994 2313 66-67 -0.8396725090047674 2313 68-69 -0.8951613631737629 2313 70-71 -0.9831012614161061 2313 72-73 -0.8453541750481008 2313 74-75 -0.6654219787259272 2313 76-77 -0.5003685955761163 2313 78-79 -0.42689667291296374 2313 80-81 -0.28438423287081704 2313 82-83 0.11707620969923127 2313 84-85 0.1949316387393516 2313 86-87 0.6211628024769205 2313 88-89 1.1006052608888695 2313 90-91 1.6325440129493707 2313 92-93 2.5648597845442573 2313 94-95 2.212498302414369 2313 96-97 1.1506029808178457 2313 98-99 0.8488961792434413 2313 100-101 0.8524388231061177 2314 1 0.3748528431584184 2314 2 -0.201504540188143 2314 3 -1.4820254718831158 2314 4 -0.29285030615612584 2314 5 -0.17496621977183935 2314 6 -3.523334448970271 2314 7 -2.8522647773675445 2314 8 -2.7642194501843633 2314 9 -3.709432781148667 2314 10-11 -2.8732022549614555 2314 12-13 -1.3567341204627397 2314 14-15 -1.3741380619501768 2314 16-17 -2.240760918178509 2314 18-19 -3.260454641405687 2314 20-21 -3.0980100322075828 2314 22-23 -4.324339152555975 2314 24-25 -3.0619246962866846 2314 26-27 -2.0275719383070836 2314 28-29 -2.1554577346493957 2314 30-31 -2.2341781406870425 2314 32-33 -2.490566937353279 2314 34-35 -1.8765303962192945 2314 36-37 -2.0612900390541213 2314 38-39 -3.365150547979482 2314 40-41 -2.9414338384624656 2314 42-43 -1.7922991240121888 2314 44-45 -2.7495353563808074 2314 46-47 -2.3712112277699298 2314 48-49 -3.0222457409748955 2314 50-51 -3.425020888013446 2314 52-53 -2.5152814824141316 2314 54-55 -3.1808930019529953 2314 56-57 -2.129010958062576 2314 58-59 -1.6282232903212375 2314 60-61 -1.9142960224315146 2314 62-63 -1.807095824265751 2314 64-65 -2.251663308889391 2314 66-67 -1.4516696368451747 2314 68-69 -1.3776841743732362 2314 70-71 -1.2446582845301535 2314 72-73 -2.4528427938714934 2314 74-75 -2.599308577268353 2314 76-77 -1.6598687030378692 2314 78-79 -2.3068203750680603 2314 80-81 -1.4841483054758875 2314 82-83 -1.2131806238913612 2314 84-85 -2.4085979913968316 2314 86-87 -1.1965429867808126 2314 88-89 0.13576479274639297 2314 90-91 1.325862822663911 2314 92-93 2.272864219783898 2314 94-95 2.164658283657408 2314 96-97 1.3420070174720689 2314 98-99 1.035574341061304 2314 100-101 0.704649604450756 2315 1 -2.7893640352370035 2315 2 -1.897120064948453 2315 3 -2.529303774975375 2315 4 -3.7771586085064364 2315 5 -4.099567425018208 2315 6 -2.8142707819546153 2315 7 -5.4423008852956265 2315 8 -3.1239908490780444 2315 9 -2.5679441122781554 2315 10-11 -3.791060428295996 2315 12-13 -3.1860283258822264 2315 14-15 -2.4977634050991497 2315 16-17 -2.5150006319531 2315 18-19 -2.2162415963861903 2315 20-21 -1.3612036465452277 2315 22-23 -0.9070273383386791 2315 24-25 -2.3628910779920034 2315 26-27 -4.257182500288238 2315 28-29 -3.463017110757164 2315 30-31 -3.456570826770651 2315 32-33 -3.994009144058804 2315 34-35 -3.727358075666629 2315 36-37 -3.0518156237469967 2315 38-39 -2.021698407471824 2315 40-41 -1.32254827916055 2315 42-43 -2.9600738286773662 2315 44-45 -6.2614884820248555 2315 46-47 -2.957037217234326 2315 48-49 -2.1912656745297134 2315 50-51 -2.8630856926761474 2315 52-53 -2.676796242972401 2315 54-55 -1.360245738488775 2315 56-57 -1.147881060203492 2315 58-59 -2.3787455362673384 2315 60-61 -2.9247687642433426 2315 62-63 -4.559603181874383 2315 64-65 -4.196063488604651 2315 66-67 -3.4894595101376744 2315 68-69 -1.814825877381402 2315 70-71 -3.432312753092347 2315 72-73 -2.4670112398291373 2315 74-75 -1.6097712204016688 2315 76-77 -3.233406661703359 2315 78-79 -1.7776642536201166 2315 80-81 -1.5000392974546486 2315 82-83 -0.9111903598418323 2315 84-85 -2.388557679120961 2315 86-87 -1.9315281066871606 2315 88-89 -1.4432934333485932 2315 90-91 -0.5346513443393768 2315 92-93 0.1844144287647147 2315 94-95 0.05273607233534605 2315 96-97 -0.08487154282734188 2315 98-99 -0.10338120826559027 2315 100-101 -0.5618722457920224 2316 1 -7.070374374604224 2316 2 -6.011505136516746 2316 3 -3.098077644010054 2316 4 -3.7411974820432476 2316 5 -1.8569773048579918 2316 6 -2.4565597253520117 2316 7 -3.2447475583791174 2316 8 -2.105085021152437 2316 9 -6.759610520201896 2316 10-11 -6.703127680756641 2316 12-13 -5.7250726330204245 2316 14-15 -4.289167447698581 2316 16-17 -4.700219683712039 2316 18-19 -3.9016888594035457 2316 20-21 -2.7081639346813517 2316 22-23 -3.3742456449179983 2316 24-25 -2.8360490871204505 2316 26-27 -4.091060228732363 2316 28-29 -4.077836870436734 2316 30-31 -6.830096768336148 2316 32-33 -4.949493976548638 2316 34-35 -5.514421446983423 2316 36-37 -3.932535653554183 2316 38-39 -6.291076182489679 2316 40-41 -3.5224877333164812 2316 42-43 -4.4805414288885 2316 44-45 -2.94022136618047 2316 46-47 -4.155688131631816 2316 48-49 -5.0995154338987945 2316 50-51 -4.469062654206251 2316 52-53 -5.878688688714011 2316 54-55 -4.840798102881607 2316 56-57 -4.132620402582479 2316 58-59 -2.917272875044624 2316 60-61 -3.0864184056256505 2316 62-63 -3.82356443253418 2316 64-65 -2.971949165631383 2316 66-67 -3.9107933816562905 2316 68-69 -2.935377310299714 2316 70-71 -2.353216593872304 2316 72-73 -2.014238550662185 2316 74-75 -2.208155112102226 2316 76-77 -2.83380409083367 2316 78-79 -4.667514286221724 2316 80-81 -5.40449329813773 2316 82-83 -3.021882134921782 2316 84-85 -1.6540915692690668 2316 86-87 -1.8953837902342165 2316 88-89 -0.8013134290017092 2316 90-91 -0.5536208230241328 2316 92-93 -0.7754421241585625 2316 94-95 -1.4900807066257489 2316 96-97 -3.2712564901990344 2316 98-99 -4.890130983159246 2316 100-101 -4.43913184774191 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 12996.0 3 3826.0 4 3673.0 5 4300.0 6 4875.0 7 5830.0 8 7326.0 9 9074.0 10 10872.0 11 13405.0 12 16290.0 13 18757.0 14 21076.0 15 24596.0 16 28312.0 17 30289.0 18 25941.0 19 22898.0 20 24134.0 21 26511.0 22 29140.0 23 32497.0 24 36819.0 25 41244.0 26 47121.0 27 53531.0 28 60185.0 29 68143.0 30 77171.0 31 85157.0 32 93925.0 33 102466.0 34 115318.0 35 130254.0 36 132444.0 37 99725.0 38 27726.0 39 449.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 46.446299164729126 18.95731807877998 13.356767052234343 21.23961570425655 2 16.361705814949033 20.975628997922954 39.37001916675073 23.292646020377283 3 19.644069703824705 27.05757045464246 29.12474439214804 24.173615449384794 4 12.9415744173352 16.39203141694456 36.09106878148097 34.57532538423927 5 13.83067814750266 38.247221674335286 31.631492325451305 16.29060785271075 6 30.70583190449981 35.274585045400364 17.937719458804644 16.081863591295186 7 28.49996603403713 34.34463483355061 19.73396563938499 17.421433493027273 8 27.630125183065356 32.37345849914872 19.819638578533098 20.176777739252834 9 29.38705111185295 15.757698090454342 19.400236008353453 35.45501478933926 10-11 25.773870133432354 26.516106202476124 25.810582467871047 21.899441196220472 12-13 27.81892391880878 24.302287222344404 26.626444067840467 21.252344791006355 14-15 24.54681826738159 24.681981987226884 26.493382831911187 24.27781691348034 16-17 22.209407774136835 28.43040547271083 26.57021548540166 22.78997126775067 18-19 22.082681679899206 25.91885155887362 30.739561280222027 21.258905481005144 20-21 23.869742881062272 24.861871840358784 30.231822398888248 21.036562879690695 22-23 23.142819328548235 25.266374763007605 29.949820227169692 21.64098568127447 24-25 22.358818503126578 25.45170311104206 30.54909309822504 21.64038528760632 26-27 22.189154220577958 25.705404244451014 30.524038628058463 21.58140290691257 28-29 21.94022053316122 25.762294694606304 30.68638729186236 21.611097480370116 30-31 21.944211048263167 26.033743693448173 30.32177817250143 21.70026708578723 32-33 21.617116584968773 26.142052378355373 29.994254984812123 22.24657605186373 34-35 22.118387522921054 25.978930430679554 30.117212100892115 21.78546994550728 36-37 22.490352645089 25.749237710889737 29.821047756308282 21.939361887712977 38-39 22.189540410813496 25.644226139005532 30.041319532540427 22.12491391764054 40-41 22.001489983067785 25.794538972571885 30.20138072627289 22.002590318087435 42-43 21.849871591079722 25.889801328618102 29.85643313157254 22.403893948729632 44-45 21.721760914789844 26.10521519908128 29.629775842447398 22.543248043681487 46-47 21.921087089773323 26.30463782768457 28.96860713710113 22.805667945440977 48-49 21.84686310725345 26.262389500146497 28.67817813037728 23.212569262222768 50-51 22.06295283183494 26.774330256475338 28.36927605506034 22.79344085662938 52-53 22.010078722822172 26.606727458449146 28.207376278254337 23.175817540474345 54-55 21.41808868250544 26.589373238162118 28.486581460649603 23.505956618682838 56-57 21.712621471095357 26.89708210431326 27.923390065968157 23.46690635862323 58-59 21.76643030840723 26.242984749405974 28.07453199025479 23.916052951932 60-61 21.772614752374324 26.316371931981813 28.315142297797983 23.595871017845877 62-63 22.0050682912119 26.16194717639491 28.14292943608142 23.690055096311767 64-65 21.923271187915923 26.212867819191878 28.20261267665562 23.661248316236573 66-67 21.790507033439052 26.41341043889139 27.8940390750569 23.902043452612666 68-69 21.90098422954656 26.345678916467335 27.97000067306597 23.783336180920134 70-71 21.915821344132404 26.552274943999336 27.56381421954072 23.968089492327536 72-73 21.960858701524536 26.54032394071219 27.195915927036463 24.302901430726816 74-75 21.905409320520974 26.829912550316344 27.158022857013375 24.106655272149307 76-77 22.07224459547967 26.530089612099623 27.37748550630561 24.020180286115096 78-79 22.01470841518704 26.747676477025063 27.30199388509099 23.935621222696906 80-81 22.08053174080485 27.080722776874406 27.11935091771785 23.719394564602894 82-83 22.236047811282976 26.88860136844191 26.607686120921777 24.26766469935334 84-85 22.30938256466565 26.493251646543857 26.73383188775857 24.463533901031916 86-87 22.29957227453287 26.416814307972047 27.007012651778123 24.27660076571696 88-89 22.476093790617245 26.298206148101794 26.810531778206315 24.41516828307464 90-91 22.320815813952283 26.442794815344318 26.97869003247221 24.25769933823119 92-93 22.64841644948982 26.139431434042766 26.93935894071397 24.27279317575345 94-95 22.674854704238843 26.12355737031926 26.79507538336469 24.406512542077206 96-97 23.24215132207008 25.931465824880277 26.05724156065538 24.76914129239426 98-99 23.351841552111903 25.889921826233515 25.845478778282637 24.912757843371942 100-101 23.901090445376163 25.867339351512715 25.888268956438882 24.34330124667224 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2901.0 1 1879.5 2 1034.0 3 1211.5 4 1052.0 5 1083.0 6 1708.0 7 3096.0 8 4676.0 9 5008.5 10 4205.0 11 3508.5 12 3333.0 13 3269.0 14 3254.5 15 3340.0 16 3360.0 17 3470.0 18 3717.5 19 3969.5 20 4250.0 21 4678.0 22 5247.0 23 5897.0 24 6574.5 25 7319.0 26 8300.0 27 9593.5 28 11056.0 29 12859.5 30 15250.5 31 17614.5 32 19874.5 33 21416.5 34 22676.0 35 24431.5 36 26416.0 37 28878.0 38 31409.0 39 33175.0 40 35013.0 41 37980.5 42 40838.5 43 43627.5 44 46549.0 45 49512.0 46 52756.0 47 56476.0 48 62305.5 49 64017.5 50 60988.0 51 59253.5 52 58360.5 53 57989.5 54 56848.5 55 54023.5 56 50322.0 57 46665.0 58 43243.5 59 39158.5 60 34126.5 61 29159.5 62 23874.5 63 17993.5 64 13064.0 65 9828.5 66 7335.5 67 5484.0 68 4068.0 69 2913.0 70 2240.5 71 1783.5 72 1445.5 73 1179.5 74 934.0 75 694.5 76 498.5 77 367.0 78 243.5 79 160.5 80 108.5 81 87.5 82 74.5 83 46.0 84 28.5 85 20.5 86 14.0 87 11.0 88 9.0 89 5.5 90 6.0 91 7.0 92 4.0 93 2.0 94 1.5 95 1.0 96 1.5 97 1.5 98 1.5 99 1.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.13505169554142102 2 0.1206487648356645 3 0.12801169802156695 4 0.13718307093734014 5 0.12600949689206717 6 0.13970196913251728 7 0.1699287474746431 8 0.15500911970320919 9 0.16715150074662727 10-11 0.16108031022491823 12-13 0.14958380051359688 14-15 0.15455700977074152 16-17 0.1484212321158228 18-19 0.16460030898484526 20-21 0.16986416034143342 22-23 0.2035463503102766 24-25 0.22450487503681466 26-27 0.23687331104646656 28-29 0.23154487255666872 30-31 0.22437570077039531 32-33 0.22466634286983883 34-35 0.2143646951228964 36-37 0.2223412060742907 38-39 0.2108124027963645 40-41 0.21391258519042872 42-43 0.20874561453365506 44-45 0.20089827784868008 46-47 0.21488139218857377 48-49 0.25147000315185214 50-51 0.2530200943488842 52-53 0.25534523114443236 54-55 0.2532784428817229 56-57 0.22834780946279007 58-59 0.2284123965959997 60-61 0.20157644274738165 62-63 0.19389057389543085 64-65 0.1952146101262291 66-67 0.2029004789781799 68-69 0.20202855267984934 70-71 0.20829350460118737 72-73 0.23648578824720856 74-75 0.238552576509918 76-77 0.2530200943488842 78-79 0.2502105540542635 80-81 0.263838439161504 82-83 0.2620945865648429 84-85 0.23877863147615183 86-87 0.24094230043867582 88-89 0.24352578576706266 90-91 0.4307961785085022 92-93 0.571208606106326 94-95 0.33265602959640794 96-97 0.27630375587097045 98-99 0.3066274149129107 100-101 0.32471181221161843 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1548296.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 50.93273731859285 #Duplication Level Percentage of deduplicated Percentage of total 1 74.78186056019291 38.08844860107944 2 12.151450629207233 12.378132858745236 3 4.83180680918107 7.382914409586232 4 2.5746936150275768 5.245447742802311 5 1.5273699522213242 3.8896566282400213 6 0.994416801665811 3.0389021846663997 7 0.6748670291332315 2.406097758387542 8 0.4614822640576934 1.8803643945952 9 0.34340474187967573 1.5741489160905033 >10 1.5287058636531716 13.316254768637744 >50 0.07992340671712057 2.786241972645666 >100 0.0459804755656978 4.351800156439756 >500 0.0023445589340293648 0.8258197133953319 >1k 0.0013025327411274247 1.0442161221756527 >5k 2.6050654822548495E-4 0.9665301578130294 >10k+ 1.3025327411274247E-4 0.8250236146998994 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12436 0.8032055885954623 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8179 0.5282581625218951 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6390 0.4127117812097945 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 2448 0.1581093020972734 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2138 0.13808729080227553 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1755 0.11335041878297172 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 1740 0.11238161178482667 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1624 0.10488950433250491 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 6.45871332096705E-5 0.0 5 0.0 0.0 0.0 6.45871332096705E-5 0.0 6 0.0 0.0 0.0 6.45871332096705E-5 0.0 7 0.0 0.0 0.0 6.45871332096705E-5 0.0 8 0.0 0.0 0.0 6.45871332096705E-5 0.0 9 0.0 0.0 0.0 6.45871332096705E-5 0.0 10-11 0.0 0.0 0.0 1.29174266419341E-4 0.0 12-13 0.0 0.0 0.0 3.2293566604835254E-4 0.0 14-15 0.0 0.0 0.0 3.5522923265318777E-4 0.0 16-17 0.0 0.0 0.0 4.521099324676935E-4 0.0 18-19 0.0 6.45871332096705E-5 0.0 5.812841988870345E-4 0.0 20-21 0.0 6.45871332096705E-5 0.0 7.427520319112108E-4 0.0 22-23 0.0 6.45871332096705E-5 0.0 0.00196990756289495 0.0 24-25 0.0 6.45871332096705E-5 0.0 0.004714860724305946 0.0 26-27 0.0 6.45871332096705E-5 0.0 0.011270454745087501 0.0 28-29 0.0 6.45871332096705E-5 0.0 0.02883815497811788 0.0 30-31 0.0 6.45871332096705E-5 0.0 0.0895177666286033 0.0 32-33 0.0 6.45871332096705E-5 0.0 0.20926231159933242 0.0 34-35 0.0 6.45871332096705E-5 0.0 0.3833892227326041 0.0 36-37 0.0 6.45871332096705E-5 0.0 0.649036101623979 0.0 38-39 0.0 6.45871332096705E-5 0.0 1.0491856854244925 3.229356660483525E-5 40-41 0.0 6.45871332096705E-5 0.0 1.5135025860688138 6.45871332096705E-5 42-43 0.0 6.45871332096705E-5 0.0 1.9409402336504131 6.45871332096705E-5 44-45 0.0 6.45871332096705E-5 0.0 2.420596578432031 6.45871332096705E-5 46-47 0.0 6.45871332096705E-5 0.0 2.9780481251646975 6.45871332096705E-5 48-49 0.0 6.45871332096705E-5 0.0 3.5728633284591576 6.45871332096705E-5 50-51 0.0 6.45871332096705E-5 0.0 4.198777236394074 6.45871332096705E-5 52-53 0.0 6.45871332096705E-5 0.0 4.785131525238068 6.45871332096705E-5 54-55 0.0 6.45871332096705E-5 0.0 5.369192970853119 6.45871332096705E-5 56-57 0.0 6.45871332096705E-5 0.0 6.007992011863365 6.45871332096705E-5 58-59 0.0 6.45871332096705E-5 0.0 6.703175620165654 6.45871332096705E-5 60-61 0.0 6.45871332096705E-5 0.0 7.4806432361770625 6.45871332096705E-5 62-63 0.0 6.45871332096705E-5 0.0 8.215741692802926 6.45871332096705E-5 64-65 0.0 6.45871332096705E-5 0.0 8.904595762050668 6.45871332096705E-5 66-67 0.0 6.45871332096705E-5 0.0 9.651255315521063 6.45871332096705E-5 68-69 0.0 6.45871332096705E-5 0.0 10.454913014048994 6.45871332096705E-5 70-71 0.0 6.45871332096705E-5 0.0 11.303265008758014 6.45871332096705E-5 72-73 0.0 6.45871332096705E-5 0.0 12.140249668022136 6.45871332096705E-5 74-75 0.0 6.45871332096705E-5 0.0 12.943907366550064 6.45871332096705E-5 76-77 0.0 6.45871332096705E-5 0.0 13.756316621627906 6.45871332096705E-5 78-79 0.0 6.45871332096705E-5 0.0 14.615390080449732 6.45871332096705E-5 80-81 0.0 6.45871332096705E-5 0.0 15.476271979001432 6.45871332096705E-5 82-83 0.0 6.45871332096705E-5 0.0 16.30169554142102 6.45871332096705E-5 84-85 0.0 6.45871332096705E-5 3.229356660483525E-5 17.086816732717775 6.45871332096705E-5 86-87 0.0 6.45871332096705E-5 6.45871332096705E-5 17.864801045794863 6.45871332096705E-5 88-89 0.0 1.6146783302417627E-4 1.29174266419341E-4 18.65095563122297 6.45871332096705E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 13915 0.0 36.277843 1 GGTATCA 8580 0.0 32.845978 1 TATCAAC 17530 0.0 29.879284 2 ATCAACG 17870 0.0 29.153381 3 TCAACGC 18005 0.0 28.90844 4 CAACGCA 18215 0.0 28.573307 5 AACGCAG 18455 0.0 28.176014 6 TACATGG 13265 0.0 27.46863 2 GTACATG 13190 0.0 27.229153 1 ACATGGG 13555 0.0 25.622572 3 ACGCAGA 20660 0.0 25.145882 7 CGCAGAG 20835 0.0 24.798845 8 GCAGAGT 20305 0.0 24.231085 9 ATGGGAG 2940 0.0 24.044846 5 ATGGGAT 3250 0.0 23.941069 5 AGTACTT 9230 0.0 23.901228 12-13 GAGTACT 9045 0.0 23.655762 12-13 CATGGGA 9240 0.0 23.569582 4 CATGGGG 4315 0.0 23.091368 4 GTACTTT 10005 0.0 21.505884 14-15 >>END_MODULE