FastQCFastQC Report
Fri 27 May 2016
SRR939767_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939767_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1548296
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT131810.8513230028366668No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT91990.5941370383957589No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77340.49951688824359164No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA27820.17968140458930335No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA25260.1631470984876277No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20710.1337599528772276No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA20230.13065977048316343No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18610.1201966549031968No Hit
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGT18160.11729023390876163No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG16630.10740840252768205No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA129050.036.9952661
GGTATCA77700.035.7616771
TATCAAC163300.029.171622
ATCAACG163950.029.0540873
TCAACGC165900.028.7125824
CAACGCA169050.028.2612635
AACGCAG172500.027.6472516
GTACATG133050.024.8912031
ACGCAGA194050.024.5526337
TACATGG133050.024.4601762
CGCAGAG195350.024.3168728
ACATGGG134800.023.2663923
GCAGAGT200750.023.1228439
AGTACTT96200.022.60774212-13
GAGTACT96400.022.36529412-13
CATGGGA93500.022.1941434
GTACTTT103950.020.58220514-15
ATGGGAG29950.020.3137535
CATGGGG41350.020.3021374
TAAGGTG6050.020.2682045
Produced by FastQC (version 0.11.5)