##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939767_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1548296 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.384952231356277 31.0 31.0 34.0 30.0 34.0 2 31.569606199331393 31.0 31.0 34.0 30.0 34.0 3 31.616409265411782 31.0 31.0 34.0 30.0 34.0 4 35.448327064075606 37.0 35.0 37.0 33.0 37.0 5 35.18736856518392 37.0 35.0 37.0 32.0 37.0 6 35.225775303947046 37.0 35.0 37.0 32.0 37.0 7 35.13087678325075 37.0 35.0 37.0 32.0 37.0 8 35.0791444271638 37.0 35.0 37.0 32.0 37.0 9 36.646504931873494 39.0 35.0 39.0 32.0 39.0 10-11 36.501552351746696 38.0 35.0 39.0 32.0 39.0 12-13 36.43914277373319 38.0 35.0 39.0 32.0 39.0 14-15 37.37644804352656 39.0 36.0 41.0 32.0 41.0 16-17 37.33287110475 39.0 36.0 41.0 32.0 41.0 18-19 37.38437288477139 39.0 36.0 41.0 32.0 41.0 20-21 37.337805238791546 39.0 36.0 41.0 31.5 41.0 22-23 37.1850298650904 39.0 36.0 40.0 31.0 41.0 24-25 36.997078401029256 39.0 36.0 40.0 31.0 41.0 26-27 36.65599697990565 38.5 35.0 40.0 30.0 41.0 28-29 36.39664121072457 38.0 35.0 40.0 30.0 41.0 30-31 36.057003957899525 38.0 35.0 40.0 30.0 41.0 32-33 35.88037461829005 38.0 34.5 40.0 29.5 41.0 34-35 35.85980490810543 38.0 35.0 40.0 29.5 41.0 36-37 35.748436345505 38.0 35.0 40.0 29.0 41.0 38-39 35.536965476885555 38.0 34.0 40.0 27.5 41.0 40-41 35.27703132992657 38.0 34.0 40.0 27.0 41.0 42-43 34.95726366276216 38.0 34.0 40.0 26.0 41.0 44-45 34.56109781333802 38.0 33.0 40.0 24.5 41.0 46-47 34.06020715677106 38.0 33.0 40.0 23.5 41.0 48-49 33.60308461689496 37.5 33.0 40.0 21.5 41.0 50-51 33.256631160966634 37.0 32.5 40.0 19.5 41.0 52-53 32.90376097335393 37.0 32.0 40.0 17.5 41.0 54-55 32.53538793615691 36.5 31.5 40.0 14.0 41.0 56-57 31.131343748223852 35.5 29.5 39.0 5.0 40.5 58-59 27.67354110583506 33.5 20.0 38.0 2.0 40.0 60-61 27.60660784501155 33.0 19.0 38.0 2.0 40.0 62-63 29.463902897120448 34.0 27.5 38.0 2.0 40.0 64-65 28.98299356195456 34.0 27.0 38.0 2.0 40.0 66-67 23.69980966171843 27.0 16.0 35.0 2.0 38.0 68-69 16.359465825656077 13.0 6.5 28.5 2.0 34.5 70-71 15.293051522447904 11.5 6.5 25.0 2.0 32.5 72-73 17.233476350775305 15.5 6.5 29.0 2.0 34.0 74-75 17.887059063641576 17.0 6.5 30.0 2.0 34.5 76-77 18.753568439109834 20.5 7.0 30.5 2.0 34.0 78-79 21.645833871559443 27.5 7.0 33.5 2.0 35.0 80-81 24.184463435932148 30.5 14.0 34.0 2.0 36.0 82-83 25.206770217064438 32.0 19.0 35.0 2.0 35.5 84-85 25.23458466598118 32.5 18.0 35.0 2.0 35.0 86-87 24.957519104874002 32.0 16.5 35.0 2.0 35.0 88-89 24.53708754656732 32.0 6.0 35.0 2.0 35.0 90-91 22.890538372507585 31.0 2.0 34.0 2.0 35.0 92-93 20.805693807902365 29.5 2.0 34.0 2.0 35.0 94-95 16.646698370337454 15.0 2.0 32.5 2.0 35.0 96-97 15.409770806099091 6.0 2.0 31.5 2.0 34.5 98-99 14.384167174752115 2.0 2.0 31.0 2.0 34.0 100-101 13.796771741320782 2.0 2.0 30.5 2.0 34.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.3931463073424517 1101 2 0.4629927904701461 1101 3 0.4669562379894039 1101 4 0.34243723693221284 1101 5 0.5092878230374538 1101 6 0.415783410422101 1101 7 0.44792121766199955 1101 8 0.5385204207384504 1101 9 0.49420134371045066 1101 10-11 0.5599488406404092 1101 12-13 0.4923112232050002 1101 14-15 0.4823191742064168 1101 16-17 0.506770109166581 1101 18-19 0.5576022427649363 1101 20-21 0.6704791354124353 1101 22-23 0.7178114057653815 1101 24-25 0.5043424597185577 1101 26-27 0.5347734968116029 1101 28-29 0.43829023576381587 1101 30-31 0.6579159910952939 1101 32-33 0.5548869195190633 1101 34-35 0.8600022693783345 1101 36-37 0.6863619409997668 1101 38-39 0.5639085328052431 1101 40-41 0.5810826415185275 1101 42-43 0.5104161996309102 1101 44-45 0.5906424563491086 1101 46-47 0.28551142094284643 1101 48-49 0.3069365997945823 1101 50-51 0.3225096462215191 1101 52-53 0.22869987630311073 1101 54-55 0.33833457326533534 1101 56-57 1.0361856069643451 1101 58-59 -7.936676956962888 1101 60-61 -9.469248092773324 1101 62-63 -2.859397511409412 1101 64-65 -0.33060497409518064 1101 66-67 -1.3092821555530598 1101 68-69 -3.674760860921632 1101 70-71 -4.282489193559504 1101 72-73 -6.5361513011339305 1101 74-75 -7.308208303135469 1101 76-77 -8.135220834276463 1101 78-79 -11.002363880863165 1101 80-81 -7.37827598431074 1101 82-83 -3.8565215492819434 1101 84-85 -2.973544554467935 1101 86-87 -2.7395327830923506 1101 88-89 -2.863003460189965 1101 90-91 -8.582172051882885 1101 92-93 -13.763534871198349 1101 94-95 -11.824548970721334 1101 96-97 -11.65493646347062 1101 98-99 -11.23636829644434 1101 100-101 -11.048063890868523 1102 1 0.47719891204112486 1102 2 0.48885251127369145 1102 3 0.50158340857163 1102 4 0.3817332873237689 1102 5 0.4387226204497807 1102 6 0.4951586232242846 1102 7 0.43259520472362567 1102 8 0.5276717656482219 1102 9 0.568162869076474 1102 10-11 0.7272688099968931 1102 12-13 0.6444307329087806 1102 14-15 0.7643991027048855 1102 16-17 0.8330426661975707 1102 18-19 0.7529014426287475 1102 20-21 0.7325471469888711 1102 22-23 0.8349887976618788 1102 24-25 0.7006077814754477 1102 26-27 0.8401680320516434 1102 28-29 0.8287545701185977 1102 30-31 0.6908258480922242 1102 32-33 0.6708853873433753 1102 34-35 0.7192804443868468 1102 36-37 0.7169650223753194 1102 38-39 0.5895639635168521 1102 40-41 0.6965065434592788 1102 42-43 0.8586307893483109 1102 44-45 0.47407155066303375 1102 46-47 0.5390290409632712 1102 48-49 0.7486309307445254 1102 50-51 0.6829241848663941 1102 52-53 0.4525230802527176 1102 54-55 0.49177345647949977 1102 56-57 1.3094257329432395 1102 58-59 -8.394710239223695 1102 60-61 -9.300685358690927 1102 62-63 -2.5487063299317114 1102 64-65 0.2752130374812616 1102 66-67 -0.7964267759139751 1102 68-69 -3.146370922208659 1102 70-71 -3.802164883038566 1102 72-73 -6.169948543217689 1102 74-75 -7.337338793431691 1102 76-77 -7.738234113132439 1102 78-79 -10.77031374466977 1102 80-81 -6.971853614545672 1102 82-83 -2.9716475281719674 1102 84-85 -2.1380023345156047 1102 86-87 -1.9243791399190435 1102 88-89 -2.1319215739114483 1102 90-91 -7.329375831249585 1102 92-93 -13.362315514712138 1102 94-95 -11.674240407561648 1102 96-97 -11.570409309912566 1102 98-99 -11.047955375096024 1102 100-101 -10.902034779530421 1103 1 0.3513749639317645 1103 2 0.3689771067003633 1103 3 0.2086690333907235 1103 4 0.17158704886891485 1103 5 0.15716590124340257 1103 6 0.22617656917591944 1103 7 0.2202155899563678 1103 8 0.3216337554911348 1103 9 0.3768161332924578 1103 10-11 0.43135878315690945 1103 12-13 0.3352215915252046 1103 14-15 0.4913408901953531 1103 16-17 0.4037903703261918 1103 18-19 0.46672420004264126 1103 20-21 0.39587389093014025 1103 22-23 0.3347042806500582 1103 24-25 0.3888618337871108 1103 26-27 0.39824486151247385 1103 28-29 0.49151392464324317 1103 30-31 0.5424699617148363 1103 32-33 0.452813787732822 1103 34-35 0.43852135957775573 1103 36-37 0.30933848631237737 1103 38-39 0.1498667685585673 1103 40-41 0.3813274786486147 1103 42-43 0.4457135525512186 1103 44-45 0.32361385653361907 1103 46-47 0.05892796341676387 1103 48-49 0.018302897431368592 1103 50-51 0.18963582605261564 1103 52-53 0.30538091384480737 1103 54-55 0.08018752905271498 1103 56-57 0.6068060384454341 1103 58-59 -6.5586306862936645 1103 60-61 -8.675590793788157 1103 62-63 -2.6337703380363386 1103 64-65 -0.3173882045259475 1103 66-67 -1.7860501280177523 1103 68-69 -4.097602568394487 1103 70-71 -4.862109163753246 1103 72-73 -7.402946596002996 1103 74-75 -7.832657419592781 1103 76-77 -8.728549022481701 1103 78-79 -11.561674788534727 1103 80-81 -8.11385720636737 1103 82-83 -4.542380430837547 1103 84-85 -3.8871800310132514 1103 86-87 -3.664038673376929 1103 88-89 -3.781005198888426 1103 90-91 -8.46287516024993 1103 92-93 -13.87077619544787 1103 94-95 -11.896184234364796 1103 96-97 -11.937440366671243 1103 98-99 -11.43669031049586 1103 100-101 -11.095764550864263 1104 1 0.159524459054726 1104 2 0.14371649708090217 1104 3 0.1342711672942798 1104 4 0.17612270187137824 1104 5 0.045316351969589164 1104 6 0.11187949844604361 1104 7 0.06709402199151526 1104 8 0.1488648466653757 1104 9 0.1820008347772415 1104 10-11 0.07441916481653266 1104 12-13 0.09734321073255359 1104 14-15 0.22187618727023306 1104 16-17 0.3793170600473559 1104 18-19 0.23044801270562232 1104 20-21 0.28150116827805505 1104 22-23 0.34374840736028034 1104 24-25 0.27805825456829325 1104 26-27 0.3798449983437777 1104 28-29 0.3846941029394131 1104 30-31 0.38451671148667543 1104 32-33 0.2845755115302495 1104 34-35 0.43620090565237746 1104 36-37 0.3531085764853117 1104 38-39 0.3719782154387943 1104 40-41 0.3447176611411322 1104 42-43 0.34490000874512106 1104 44-45 0.44415212428810236 1104 46-47 0.4765475211525043 1104 48-49 0.5037869035416946 1104 50-51 0.5263230612708867 1104 52-53 0.5383635592592242 1104 54-55 0.45703070071368046 1104 56-57 1.1637833615653506 1104 58-59 -5.482879222074079 1104 60-61 -7.979961674480403 1104 62-63 -2.0595357208246767 1104 64-65 0.0831669109289237 1104 66-67 -1.196208709680974 1104 68-69 -3.769467462717234 1104 70-71 -4.456326621654769 1104 72-73 -7.118943220923189 1104 74-75 -8.215351064277343 1104 76-77 -8.662132693853906 1104 78-79 -11.513642396355163 1104 80-81 -8.03450145605957 1104 82-83 -4.128618255552219 1104 84-85 -3.245926639660489 1104 86-87 -2.999279346897165 1104 88-89 -3.428195967761244 1104 90-91 -8.659358090267919 1104 92-93 -13.951260665510482 1104 94-95 -11.80051554061901 1104 96-97 -11.515504667689031 1104 98-99 -10.873557829265156 1104 100-101 -10.580072922641008 1105 1 0.10642616473995758 1105 2 0.1820324517892189 1105 3 0.07352932176836902 1105 4 0.08255532962383683 1105 5 0.11746518489128732 1105 6 -0.06332755214474162 1105 7 -0.05236844365707327 1105 8 0.08610286280618595 1105 9 0.06464474834503164 1105 10-11 0.1903354270931743 1105 12-13 0.16440346919430482 1105 14-15 0.27033722233476 1105 16-17 0.13350807708101797 1105 18-19 0.17483152083980968 1105 20-21 0.30660324414684226 1105 22-23 0.33397673553365337 1105 24-25 0.2644193313763097 1105 26-27 0.35410281944975 1105 28-29 0.22049834628074905 1105 30-31 0.15980993048923864 1105 32-33 0.22954712451015524 1105 34-35 0.2826961196457134 1105 36-37 0.17486461479655446 1105 38-39 0.2465678465307377 1105 40-41 0.2030746201281488 1105 42-43 0.25678429232252853 1105 44-45 0.2582338110728202 1105 46-47 0.11457224981985092 1105 48-49 0.1466536229674844 1105 50-51 0.14952613463506736 1105 52-53 0.1365875768378686 1105 54-55 0.13949321854163088 1105 56-57 0.9159538779090894 1105 58-59 -6.379825352684808 1105 60-61 -8.124824073165485 1105 62-63 -2.1328210853844567 1105 64-65 0.032479918952951436 1105 66-67 -1.3041239559095636 1105 68-69 -3.799894550761204 1105 70-71 -4.400782419940967 1105 72-73 -5.672843591686515 1105 74-75 -6.365160174793223 1105 76-77 -8.05714039755632 1105 78-79 -11.301494896905943 1105 80-81 -7.757726741886497 1105 82-83 -3.9094125920626936 1105 84-85 -3.0728482532201653 1105 86-87 -2.777316874001439 1105 88-89 -2.8212253853236575 1105 90-91 -7.990293709636896 1105 92-93 -10.641286653729543 1105 94-95 -11.030052535783723 1105 96-97 -11.456345777196109 1105 98-99 -11.081098688601202 1105 100-101 -10.772428864130553 1106 1 0.01800303281722293 1106 2 0.08909591067092038 1106 3 0.13150000695220854 1106 4 0.2170055404869231 1106 5 0.07622418292807964 1106 6 0.19582423897961831 1106 7 0.20608880203477753 1106 8 0.2086874807077379 1106 9 0.13083560973285557 1106 10-11 0.22138130496224306 1106 12-13 0.26422941626039886 1106 14-15 0.27473967089144224 1106 16-17 0.25440139732630485 1106 18-19 0.39659866558398704 1106 20-21 0.2563085357195831 1106 22-23 0.20649318648980852 1106 24-25 0.22845331487082632 1106 26-27 0.4573855880578108 1106 28-29 0.34896652898027725 1106 30-31 0.3434986408312213 1106 32-33 0.36051817022139687 1106 34-35 0.4186656959805717 1106 36-37 0.3689025822703158 1106 38-39 0.4028712439712905 1106 40-41 0.4655219043570469 1106 42-43 0.3842428468197383 1106 44-45 0.35205197801820987 1106 46-47 0.1804476497325922 1106 48-49 0.23862976756623766 1106 50-51 0.3864812535182196 1106 52-53 0.2605044798166176 1106 54-55 -0.10787099290315183 1106 56-57 0.7271026575692829 1106 58-59 -5.761236832604343 1106 60-61 -8.206845950210493 1106 62-63 -2.0478339462148227 1106 64-65 0.27660885142828917 1106 66-67 -1.0690805411394528 1106 68-69 -3.1224923476055295 1106 70-71 -3.955705285255201 1106 72-73 -5.532610172917277 1106 74-75 -5.776911247286497 1106 76-77 -7.544919957773486 1106 78-79 -10.983490037360149 1106 80-81 -6.858616472005984 1106 82-83 -2.9341871109555413 1106 84-85 -1.9767476297198385 1106 86-87 -1.7912937487439216 1106 88-89 -2.02209671036789 1106 90-91 -6.934544509462389 1106 92-93 -10.633280067287796 1106 94-95 -10.678929144523634 1106 96-97 -11.303818828530364 1106 98-99 -10.925506120919 1106 100-101 -10.645277060887626 1107 1 0.0693293517658411 1107 2 0.046998234239538306 1107 3 0.0512728870056236 1107 4 -0.10902124735888918 1107 5 0.01893983054353754 1107 6 0.04284340336192827 1107 7 0.013900445872984335 1107 8 -0.011280779491933401 1107 9 0.008679819455025495 1107 10-11 0.09664760325119204 1107 12-13 0.02283906446245254 1107 14-15 0.1781555266951358 1107 16-17 0.1494319358949312 1107 18-19 0.08443208316774076 1107 20-21 0.05378390289102697 1107 22-23 0.11970116479033521 1107 24-25 0.12722510282652166 1107 26-27 0.22872450288706858 1107 28-29 0.24939654719201343 1107 30-31 0.22739372366315536 1107 32-33 0.32127048230820776 1107 34-35 -0.05014093148374599 1107 36-37 0.049742140914297295 1107 38-39 0.19401020866872187 1107 40-41 0.024947253301597527 1107 42-43 0.3924034541503545 1107 44-45 0.40649069966516294 1107 46-47 0.34491237406697195 1107 48-49 0.340260411176331 1107 50-51 0.4290607489099614 1107 52-53 0.44868784613014157 1107 54-55 0.3517321913839808 1107 56-57 0.9980436880820847 1107 58-59 -6.221995965565156 1107 60-61 -8.294273639472692 1107 62-63 -2.2191612276645465 1107 64-65 0.042246084002663054 1107 66-67 -1.3444234984733718 1107 68-69 -3.7576095036951678 1107 70-71 -4.722489750942037 1107 72-73 -5.7458532408251415 1107 74-75 -6.122767831589847 1107 76-77 -8.34347775975442 1107 78-79 -11.360944320452187 1107 80-81 -7.6803745295423305 1107 82-83 -3.8743589515077588 1107 84-85 -2.92044434637846 1107 86-87 -2.7085866726934498 1107 88-89 -2.8548598691601086 1107 90-91 -8.11954385390061 1107 92-93 -11.535963247543034 1107 94-95 -11.41705798173893 1107 96-97 -11.843453222105405 1107 98-99 -11.186544568669968 1107 100-101 -10.67355299404003 1108 1 -0.01583089770384305 1108 2 0.02200744565811874 1108 3 -0.020507891091760655 1108 4 -0.0438975222137401 1108 5 -0.2025716892985301 1108 6 -0.0548385326536831 1108 7 0.035794591062469294 1108 8 -0.02757321363987586 1108 9 0.0653020169105929 1108 10-11 0.09702245047309788 1108 12-13 -0.05364145425452449 1108 14-15 0.06597286417338921 1108 16-17 -0.03157545186361688 1108 18-19 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9.085262533355783 2313 100-101 7.9419476723713895 2314 1 -0.4209299389918577 2314 2 -0.3873324219518395 2314 3 -0.48044394247529354 2314 4 -0.25195331874866156 2314 5 -0.5090218243044475 2314 6 -0.43349097111034496 2314 7 -0.5494582845562164 2314 8 -0.5948241916384163 2314 9 -0.5560599541314915 2314 10-11 -0.5510397675256158 2314 12-13 -0.4366082444241428 2314 14-15 -0.7088606137475608 2314 16-17 -0.8426480801709602 2314 18-19 -0.7459685144348498 2314 20-21 -0.6468501750620348 2314 22-23 -0.5795897146196083 2314 24-25 -0.736682241386788 2314 26-27 -0.7227214276188008 2314 28-29 -0.8308916576116232 2314 30-31 -1.0172475550359934 2314 32-33 -0.8647357964746192 2314 34-35 -0.8873687735572204 2314 36-37 -0.9312117487513447 2314 38-39 -0.7909198377489304 2314 40-41 -0.9424059666474989 2314 42-43 -0.9089123213105097 2314 44-45 -1.0521633937148067 2314 46-47 -0.6666344170900871 2314 48-49 -0.8434497074465668 2314 50-51 -0.620064983449879 2314 52-53 -0.6526737232156492 2314 54-55 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2315 14-15 -0.8673637010578474 2315 16-17 -1.0256810636915645 2315 18-19 -0.9717255031325678 2315 20-21 -0.9035229284152138 2315 22-23 -0.7702750233094164 2315 24-25 -0.9485380899643232 2315 26-27 -0.9641618701470165 2315 28-29 -0.816454050957212 2315 30-31 -0.8816490949814195 2315 32-33 -0.6579604075724461 2315 34-35 -0.8677146842107106 2315 36-37 -0.7646661975283777 2315 38-39 -0.9418915779367296 2315 40-41 -0.6293296206379182 2315 42-43 -0.6533510408805157 2315 44-45 -0.4414425129451658 2315 46-47 -0.2744893341160477 2315 48-49 -0.36721395894900155 2315 50-51 -0.2767453623358165 2315 52-53 -0.31822074183843085 2315 54-55 -0.18572663684238222 2315 56-57 1.1969087016990656 2315 58-59 4.427022328248025 2315 60-61 4.040441829707294 2315 62-63 1.6796453255985426 2315 64-65 1.442735154768208 2315 66-67 1.9450279723035813 2315 68-69 5.987346508453179 2315 70-71 5.093907665395493 2315 72-73 8.78130943580355 2315 74-75 9.864356631929464 2315 76-77 8.768067447209493 2315 78-79 6.295398627945858 2315 80-81 3.410050805178841 2315 82-83 1.686205975131628 2315 84-85 1.347741814202152 2315 86-87 1.5001367527981166 2315 88-89 1.587116090801679 2315 90-91 2.7934357705272603 2315 92-93 4.551320912552782 2315 94-95 8.083803239084364 2315 96-97 8.64114603929793 2315 98-99 9.077875889602174 2315 100-101 8.4862761876576 2316 1 -0.45342487848106927 2316 2 -0.5310973094610745 2316 3 -0.5574672357331352 2316 4 -0.3926297210319376 2316 5 -0.4406858792726922 2316 6 -0.47047908098057434 2316 7 -0.48338921645217425 2316 8 -0.7494008480239387 2316 9 -0.728652360218021 2316 10-11 -0.739803859930042 2316 12-13 -0.805534389126592 2316 14-15 -0.8883265352243299 2316 16-17 -0.7985860222409542 2316 18-19 -1.081282431750573 2316 20-21 -0.8864396856419035 2316 22-23 -0.7867062339158082 2316 24-25 -2.248496173610725 2316 26-27 -1.5253581939275662 2316 28-29 -1.2936422398602545 2316 30-31 -0.8377145465913216 2316 32-33 -0.5785258126086177 2316 34-35 -0.5740254241119729 2316 36-37 -0.6018491080757684 2316 38-39 -0.9691744667497204 2316 40-41 -0.8122448254070704 2316 42-43 -0.7625012256229624 2316 44-45 -0.5464150344467029 2316 46-47 -0.4576870861575344 2316 48-49 -0.5198764236305919 2316 50-51 -0.45767807882602085 2316 52-53 -0.4603668591421268 2316 54-55 -0.47787896397623797 2316 56-57 0.6746697474344678 2316 58-59 3.6924335742504475 2316 60-61 3.7023584937865337 2316 62-63 1.2987653926647056 2316 64-65 0.8355069124606338 2316 66-67 5.821626227341305 2316 68-69 8.455823858097666 2316 70-71 5.2374525469429845 2316 72-73 8.796748626041698 2316 74-75 9.633715777456835 2316 76-77 8.311901085859475 2316 78-79 6.1344801940650235 2316 80-81 3.1849537765825744 2316 82-83 1.9992078070315245 2316 84-85 1.5750045210671217 2316 86-87 1.5953536311164918 2316 88-89 1.626404133773704 2316 90-91 2.9088144149213377 2316 92-93 4.730443463551012 2316 94-95 8.49592327510138 2316 96-97 9.27504239723254 2316 98-99 9.734242517974886 2316 100-101 8.77177468177379 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 8.0 3 8.0 4 46.0 5 132.0 6 274.0 7 786.0 8 1580.0 9 2660.0 10 4437.0 11 6706.0 12 9576.0 13 12488.0 14 15645.0 15 20969.0 16 27708.0 17 34415.0 18 33705.0 19 31395.0 20 31891.0 21 31204.0 22 29874.0 23 28813.0 24 29734.0 25 30549.0 26 39180.0 27 56054.0 28 78118.0 29 99390.0 30 115166.0 31 121071.0 32 122332.0 33 125211.0 34 125597.0 35 118170.0 36 94182.0 37 53456.0 38 15228.0 39 538.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.45506043686639 19.127197461981073 14.420221462952663 21.997520638199873 2 16.218822502053957 21.833161955055832 38.39301861736484 23.554996925525373 3 19.348085989196772 27.0653599794864 29.723925409545416 23.862628621771417 4 13.183073520825475 17.054813808212383 35.64357203015444 34.1185406408077 5 13.618249784784176 38.749188901326455 31.754997789675325 15.877563524214041 6 30.85413309479137 35.745319851837024 17.43375777871788 15.966789274653731 7 28.206815750993353 34.394004763946946 19.58469181603518 17.81448766902453 8 27.577708109700094 32.90699311629445 19.64039129519329 19.874907478812165 9 28.67584751236198 15.748991148979266 19.31723649741393 36.25792484124483 10-11 25.650553899254408 26.70061151097723 25.797683388706034 21.85115120106233 12-13 27.378324299746303 24.334558766540766 26.802691475015113 21.48442545869782 14-15 24.02425638291448 24.9652278460124 26.438075209900447 24.57244056117267 16-17 22.000056836677224 28.668775221275517 26.51414845740091 22.817019484646348 18-19 21.92909652942567 26.153155061700968 30.51827389725591 21.399474511617452 20-21 23.555024362266646 25.136698667438267 30.05219932106006 21.25607764923503 22-23 22.824285537132436 25.602630246412833 29.773925657626187 21.799158558828545 24-25 22.16633641112552 25.82322759989046 30.3236590419403 21.686776947043718 26-27 22.140167778158485 26.055378999279533 30.26346085967495 21.540992362887035 28-29 21.802411668032175 26.04364435146579 30.43472223253843 21.71922174796361 30-31 21.865167668403153 26.309292734207713 30.1475959939153 21.677943603473835 32-33 21.457003644010765 26.43490189847665 29.776547606591514 22.33154685092108 34-35 21.92563882137674 26.21772146012255 30.043609654836555 21.813030063664154 36-37 22.371263697997357 26.002129399801273 29.624790193390332 22.001816708811038 38-39 22.061453322428054 25.782492631829207 29.918714313955785 22.237339731786953 40-41 21.831027318139647 25.930718758318065 30.121311301714137 22.116942621828155 42-43 21.74256862833774 26.06058158841733 29.741799810056662 22.45504997318827 44-45 21.545373992251683 26.279582341481035 29.47061979772189 22.704423868545394 46-47 21.834653328134547 26.406830613714398 28.772645499586027 22.98587055856503 48-49 21.69966167770772 26.331249602254058 28.599911680211292 23.369177039826926 50-51 21.853775392004156 26.794891590363328 28.23799534718153 23.113337670450985 52-53 21.890403596018952 26.55862588456614 28.213444500863734 23.337526018551177 54-55 21.315492662961326 26.57431739313556 28.378183711489914 23.732006232413198 56-57 21.61821637425955 26.820875833991707 27.831804274422765 23.729103517325974 58-59 19.99469036222661 26.254532062143582 29.610763208374653 24.14001436725515 60-61 21.645970468118765 26.47598676315644 28.091174476743376 23.78686829198142 62-63 21.61106509267467 26.427564559022276 27.937712027927542 24.02365832037551 64-65 20.848584732667412 26.362794325864975 28.60336745722763 24.185253484239986 66-67 20.7500609070833 26.335076716728363 28.628031928409946 24.2868304477784 68-69 21.324539644904466 25.858465976147844 28.60789229445477 24.209102084492915 70-71 20.619505820860063 26.180869037248222 28.636486629180858 24.563138512710857 72-73 20.301540297415645 26.09720165104463 28.558539623459467 25.042718428080256 74-75 20.844385318351275 26.22895504759144 28.0224671867125 24.904192447344787 76-77 19.272006984198708 26.515533900070327 28.850924315863914 25.36153479986705 78-79 20.031189086349148 26.648314677062597 27.889587132118436 25.43090910446982 80-81 20.524700428148932 26.896943924696426 27.269773873549774 25.308581773604864 82-83 20.732600117484186 26.582750621894363 26.62205195591146 26.062597304709996 84-85 20.653622210237714 26.139559967577235 26.764957582372865 26.441860239812183 86-87 20.728479836770706 26.1327374783748 26.851063925983603 26.28771875887089 88-89 20.65997737511993 26.0053071264497 26.86131297287889 26.47340252555149 90-91 20.35874247886509 26.14039192916807 26.967599447600538 26.53326614436631 92-93 19.95000166596416 25.84741773938045 27.302791088418054 26.899789506237337 94-95 18.92971029164474 25.811588007118825 28.071137202849496 27.187564498386944 96-97 18.630430936689933 25.58854393359348 28.377675307462724 27.403349822253865 98-99 18.9560526474574 25.526711034211715 27.86864553962665 27.64859077870423 100-101 18.8128856135937 25.606136747536773 28.003773866300087 27.577203772569437 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 946.0 1 725.0 2 533.5 3 637.5 4 746.0 5 936.0 6 1348.5 7 2101.0 8 2777.5 9 3034.0 10 3093.0 11 3182.5 12 3440.5 13 3673.5 14 3827.5 15 3859.0 16 3895.5 17 3979.0 18 4069.5 19 4234.0 20 4487.5 21 4913.0 22 5399.5 23 5891.0 24 6482.5 25 7258.0 26 8272.0 27 9451.5 28 10995.0 29 12945.0 30 15134.5 31 17336.5 32 19522.5 33 21684.0 34 23590.5 35 25695.5 36 28018.5 37 30427.5 38 32759.0 39 35108.5 40 37992.0 41 41048.5 42 44113.0 43 47351.0 44 50470.0 45 53513.5 46 56642.5 47 59999.5 48 63563.5 49 64934.0 50 63922.0 51 62297.0 52 60431.5 53 58778.0 54 56727.5 55 53631.0 56 49774.5 57 45399.0 58 40571.0 59 35351.0 60 29961.0 61 24522.5 62 19133.5 63 14215.0 64 10287.5 65 7531.0 66 5530.5 67 4012.5 68 2812.5 69 1980.0 70 1499.5 71 1145.0 72 901.0 73 734.5 74 552.0 75 383.0 76 235.5 77 143.5 78 94.0 79 62.0 80 35.0 81 20.5 82 20.0 83 18.5 84 9.0 85 4.0 86 2.5 87 1.0 88 1.0 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.020474121227465548 2 0.004133576525418912 3 0.0035522923265318774 4 0.0 5 0.06536217880818654 6 6.45871332096705E-5 7 0.0 8 1.29174266419341E-4 9 0.0 10-11 0.0 12-13 0.0 14-15 1.6146783302417627E-4 16-17 0.0 18-19 0.001420916930612751 20-21 0.0 22-23 0.0 24-25 0.0 26-27 9.688069981450575E-5 28-29 0.002034494696104621 30-31 0.009752657114660244 32-33 1.29174266419341E-4 34-35 9.688069981450576E-4 36-37 0.028547512878674363 38-39 0.027255770214480952 40-41 0.02971008127644843 42-43 0.030097604075706455 44-45 0.030775768974407997 46-47 0.03226127303823042 48-49 0.03303631863674646 50-51 0.026932834548432597 52-53 0.02338054222190072 54-55 0.01679265463451433 56-57 0.1416072895622026 58-59 0.7406852436485013 60-61 0.2954538408676377 62-63 2.2605496623384679E-4 64-65 0.14616068245348435 66-67 1.3808729080227553 68-69 2.643357600872185 70-71 2.134992275378868 72-73 1.8192580746833937 74-75 1.5174424011946037 76-77 1.0875827361176416 78-79 0.41403581744059276 80-81 1.937613996290115E-4 82-83 1.6146783302417627E-4 84-85 6.45871332096705E-5 86-87 0.0028741274278303373 88-89 3.229356660483525E-5 90-91 0.21956395934627487 92-93 0.17080067377297364 94-95 1.270590378067243 96-97 1.2458535060479392 98-99 0.986148643411854 100-101 0.39469197104429643 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1548296.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 43.959257758418616 #Duplication Level Percentage of deduplicated Percentage of total 1 69.78588678227354 30.677357849617813 2 13.673433217043987 12.021479504611195 3 5.935543098610186 7.827662070240235 4 3.174725889055315 5.582343746772292 5 1.9278134165014906 4.237262344306333 6 1.2771916355045685 3.368663778722494 7 0.8313402907772274 2.558157148906438 8 0.6301620963791974 2.216116641945486 9 0.43886506423627647 1.736296423193468 >10 2.1353866946863485 16.206326515757496 >50 0.1182457422930863 3.557784811812524 >100 0.06494140286342953 5.421848120596109 >500 0.003966956453069753 1.1451892156265726 >1k 0.0020569403830732053 1.4985438035625593 >5k 2.9384862615331505E-4 1.09364891930544 >10k+ 1.4692431307665753E-4 0.8513191050236226 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13181 0.8513230028366668 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9199 0.5941370383957589 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7734 0.49951688824359164 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2782 0.17968140458930335 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 2526 0.1631470984876277 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2071 0.1337599528772276 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 2023 0.13065977048316343 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1861 0.1201966549031968 No Hit CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGT 1816 0.11729023390876163 No Hit GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG 1663 0.10740840252768205 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 6.45871332096705E-5 0.0 5 0.0 0.0 0.0 6.45871332096705E-5 0.0 6 0.0 0.0 0.0 6.45871332096705E-5 0.0 7 0.0 0.0 0.0 6.45871332096705E-5 0.0 8 0.0 0.0 0.0 6.45871332096705E-5 0.0 9 0.0 0.0 0.0 6.45871332096705E-5 0.0 10-11 0.0 0.0 0.0 1.29174266419341E-4 0.0 12-13 0.0 0.0 0.0 3.2293566604835254E-4 6.45871332096705E-5 14-15 0.0 0.0 0.0 3.5522923265318777E-4 6.45871332096705E-5 16-17 0.0 0.0 0.0 5.16697065677364E-4 6.45871332096705E-5 18-19 0.0 0.0 0.0 6.458713320967051E-4 6.45871332096705E-5 20-21 0.0 0.0 0.0 8.073391651208813E-4 6.45871332096705E-5 22-23 0.0 0.0 0.0 0.001937613996290115 6.45871332096705E-5 24-25 0.0 0.0 0.0 0.004844034990725288 9.688069981450575E-5 26-27 0.0 0.0 0.0 0.011754858244160032 1.29174266419341E-4 28-29 0.0 0.0 0.0 0.03006531050910162 1.29174266419341E-4 30-31 0.0 0.0 0.0 0.0940711595198851 1.29174266419341E-4 32-33 0.0 0.0 0.0 0.21833680381529114 1.29174266419341E-4 34-35 0.0 0.0 0.0 0.4001495838005136 1.29174266419341E-4 36-37 0.0 0.0 0.0 0.6766793946377179 1.29174266419341E-4 38-39 0.0 0.0 0.0 1.0905860378118912 1.29174266419341E-4 40-41 0.0 0.0 0.0 1.571469538124493 1.29174266419341E-4 42-43 0.0 0.0 0.0 2.01405286844376 1.29174266419341E-4 44-45 0.0 0.0 0.0 2.5121165461901342 1.29174266419341E-4 46-47 0.0 0.0 0.0 3.070020202855268 1.29174266419341E-4 48-49 0.0 0.0 0.0 3.607417444726331 1.29174266419341E-4 50-51 0.0 0.0 0.0 4.13871120250908 1.29174266419341E-4 52-53 0.0 0.0 0.0 4.6277003880394965 1.29174266419341E-4 54-55 0.0 0.0 0.0 5.125667185086056 1.29174266419341E-4 56-57 0.0 0.0 0.0 5.532985940672843 1.29174266419341E-4 58-59 0.0 0.0 0.0 5.809806393609491 1.29174266419341E-4 60-61 0.0 0.0 0.0 6.0257534734960245 1.29174266419341E-4 62-63 0.0 0.0 0.0 6.204659832486811 1.29174266419341E-4 64-65 0.0 0.0 0.0 6.378334633687615 1.29174266419341E-4 66-67 3.229356660483525E-5 0.0 0.0 6.515259356092117 1.29174266419341E-4 68-69 6.45871332096705E-5 0.0 0.0 6.674402052320745 1.29174266419341E-4 70-71 6.45871332096705E-5 0.0 0.0 6.970178828854431 1.29174266419341E-4 72-73 6.45871332096705E-5 0.0 0.0 7.338583836682392 1.29174266419341E-4 74-75 6.45871332096705E-5 0.0 0.0 7.758497083245064 1.29174266419341E-4 76-77 6.45871332096705E-5 0.0 0.0 8.352537240941007 1.29174266419341E-4 78-79 6.45871332096705E-5 0.0 0.0 9.181287040720896 1.29174266419341E-4 80-81 6.45871332096705E-5 0.0 0.0 10.09390323297354 1.29174266419341E-4 82-83 6.45871332096705E-5 0.0 0.0 10.96221265184435 1.29174266419341E-4 84-85 6.45871332096705E-5 0.0 0.0 11.659527635542558 1.29174266419341E-4 86-87 6.45871332096705E-5 0.0 0.0 12.264935128683405 1.29174266419341E-4 88-89 6.45871332096705E-5 0.0 0.0 12.854551067754485 1.29174266419341E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 12905 0.0 36.995266 1 GGTATCA 7770 0.0 35.761677 1 TATCAAC 16330 0.0 29.17162 2 ATCAACG 16395 0.0 29.054087 3 TCAACGC 16590 0.0 28.712582 4 CAACGCA 16905 0.0 28.261263 5 AACGCAG 17250 0.0 27.647251 6 GTACATG 13305 0.0 24.891203 1 ACGCAGA 19405 0.0 24.552633 7 TACATGG 13305 0.0 24.460176 2 CGCAGAG 19535 0.0 24.316872 8 ACATGGG 13480 0.0 23.266392 3 GCAGAGT 20075 0.0 23.122843 9 AGTACTT 9620 0.0 22.607742 12-13 GAGTACT 9640 0.0 22.365294 12-13 CATGGGA 9350 0.0 22.194143 4 GTACTTT 10395 0.0 20.582205 14-15 ATGGGAG 2995 0.0 20.313753 5 CATGGGG 4135 0.0 20.302137 4 TAAGGTG 605 0.0 20.268204 5 >>END_MODULE