FastQCFastQC Report
Fri 27 May 2016
SRR939766_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939766_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1607513
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT132370.8234459068138174No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83360.5185650131600802No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67820.4218939442480403No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA25520.15875454817472703No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA22460.13971893228857246No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19670.12236292956884331No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA19500.12130539535294582No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16970.10556679790458927No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA140550.036.081651
GGTATCA87150.033.953281
TATCAAC178100.028.6336942
TCAACGC180500.028.3850574
ATCAACG180250.028.3461233
CAACGCA181950.028.2114455
GTACATG139650.027.9599021
TACATGG139400.027.605942
AACGCAG186300.027.5779766
ACATGGG141650.025.8737873
CATGGGA97650.024.9572414
GTCGGGA9500.024.7604452
ATGGGAG33300.024.301715
ACGCAGA212550.023.906467
CGCAGAG213700.023.4915968
GAGTACT96900.023.37713412-13
ATGGGAT35450.022.827845
AGTACTT97800.022.77717812-13
GCAGAGT211450.022.4510219
GTACTTT105650.021.57865114-15
Produced by FastQC (version 0.11.5)