FastQCFastQC Report
Fri 27 May 2016
SRR939766_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939766_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1607513
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT132210.8224505804929727No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT94010.5848164213913045No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76260.47439740767259736No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA29100.18102497460362685No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA25710.1599364981807301No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21380.13300047962287084No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA19940.1240425427352687No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18930.11775954533493663No Hit
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGT17890.11128992424944618No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG17220.10712199528090907No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA93350.033.354261
GTATCAA142300.032.9544331
GTACATG143250.026.1754591
TACATGG143850.025.4306622
TATCAAC184450.025.3893552
ATCAACG184400.025.3376833
TCAACGC185100.025.190614
CAACGCA187700.024.81645
AACGCAG192100.024.272686
ACATGGG143850.024.2364523
CATGGGA99700.022.313584
AGTACTT101900.022.27405712-13
CGTCACT3950.021.629041
ACGCAGA215450.021.5980287
CGCAGAG216850.021.3492168
GAGTACT101050.021.24094412-13
ATGGGAG31750.021.0652665
CATGGGG43050.020.9349544
GTCGGGA8700.020.7241862
GCAGAGT221500.020.3228249
Produced by FastQC (version 0.11.5)