##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939766_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1607513 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.356759167733014 31.0 31.0 34.0 30.0 34.0 2 31.560671671084464 31.0 31.0 34.0 30.0 34.0 3 31.591038455054484 31.0 31.0 34.0 30.0 34.0 4 35.42510013915906 37.0 35.0 37.0 33.0 37.0 5 35.16000928141794 37.0 35.0 37.0 32.0 37.0 6 35.20346958313868 37.0 35.0 37.0 32.0 37.0 7 35.09927509139895 37.0 35.0 37.0 32.0 37.0 8 35.06320197721573 37.0 35.0 37.0 32.0 37.0 9 36.6120877405035 39.0 35.0 39.0 32.0 39.0 10-11 36.47864434066785 38.0 35.0 39.0 32.0 39.0 12-13 36.391498544646296 38.0 35.0 39.0 32.0 39.0 14-15 37.30984632783685 39.0 36.0 41.0 32.0 41.0 16-17 37.26246350729356 39.0 36.0 40.5 32.0 41.0 18-19 37.31686306735933 39.0 36.0 40.5 32.0 41.0 20-21 37.263712019747274 39.0 36.0 40.5 31.0 41.0 22-23 37.121943959395665 39.0 36.0 40.0 31.0 41.0 24-25 36.947407890324996 39.0 36.0 40.0 31.0 41.0 26-27 36.60377614364549 38.5 35.0 40.0 30.0 41.0 28-29 36.34106567100857 38.0 35.0 40.0 30.0 41.0 30-31 35.988903977759435 38.0 34.5 40.0 29.5 41.0 32-33 35.83996116983191 38.0 34.0 40.0 29.5 41.0 34-35 35.61797229633602 38.0 34.0 40.0 29.0 41.0 36-37 35.5484300904565 38.0 34.0 40.0 28.0 41.0 38-39 35.3754448642095 38.0 34.0 40.0 27.0 41.0 40-41 35.20176228745895 38.0 34.0 40.0 27.0 41.0 42-43 34.86974351062791 38.0 33.5 40.0 26.0 41.0 44-45 34.46879745295995 38.0 33.0 40.0 24.5 41.0 46-47 33.991645168654934 38.0 33.0 40.0 23.0 41.0 48-49 33.54330882549628 37.0 33.0 40.0 21.0 41.0 50-51 33.23428799642678 37.0 32.5 40.0 19.5 41.0 52-53 32.91445076960497 37.0 32.0 40.0 18.0 41.0 54-55 32.600554707800185 36.0 31.0 40.0 16.0 41.0 56-57 32.197738058727985 36.0 31.0 39.0 13.0 41.0 58-59 31.79343557408245 35.0 31.0 39.0 9.0 40.0 60-61 31.42500496107963 35.0 30.0 39.0 7.5 40.0 62-63 31.081791873533838 35.0 30.0 38.5 6.5 40.0 64-65 30.60123743944839 35.0 29.0 38.0 2.0 40.0 66-67 29.67409594821317 34.0 28.0 37.5 2.0 40.0 68-69 27.78559644618737 33.0 23.0 36.0 2.0 39.0 70-71 27.14463366703722 32.5 21.5 36.0 2.0 39.0 72-73 27.688493654483665 33.0 25.0 35.0 2.0 38.5 74-75 27.786736405864215 33.0 26.0 35.0 2.0 37.5 76-77 27.31580771041976 32.0 25.5 35.0 2.0 36.5 78-79 27.823959744026954 33.0 26.0 35.0 2.0 37.0 80-81 27.64320786208261 33.0 26.0 35.0 2.0 36.0 82-83 27.22355837868807 33.0 25.5 35.0 2.0 36.0 84-85 26.894613293951586 33.0 25.0 35.0 2.0 35.5 86-87 26.5171728004688 33.0 24.5 35.0 2.0 35.0 88-89 26.087213291587688 32.0 23.5 35.0 2.0 35.0 90-91 25.597987387971358 32.0 22.0 34.0 2.0 35.0 92-93 25.1315917818394 32.0 19.5 34.0 2.0 35.0 94-95 24.348580695770423 31.0 15.5 34.0 2.0 35.0 96-97 23.848762031784503 31.0 4.5 34.0 2.0 35.0 98-99 23.20842755237438 31.0 2.0 34.0 2.0 35.0 100-101 21.78190689593179 30.0 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.26665372066920057 1101 2 0.2718626399401032 1101 3 0.315891540268904 1101 4 0.36638312663196615 1101 5 0.23533664536300591 1101 6 0.253373498542075 1101 7 0.22926545043566193 1101 8 0.2779085316652825 1101 9 0.48879064093992497 1101 10-11 0.3148914864856067 1101 12-13 0.3847503238351564 1101 14-15 0.4190234539214259 1101 16-17 0.49021748504905815 1101 18-19 0.3368438087855097 1101 20-21 0.383548938182372 1101 22-23 0.30258504883175874 1101 24-25 0.4323050970311044 1101 26-27 0.304248659366408 1101 28-29 0.35938718813284254 1101 30-31 0.2585468330566272 1101 32-33 0.30634691481758125 1101 34-35 0.05428724565508247 1101 36-37 0.4072168412459902 1101 38-39 0.5707255908510689 1101 40-41 0.4554591106221153 1101 42-43 0.277495362252715 1101 44-45 0.29183925268264943 1101 46-47 0.43588054459775805 1101 48-49 0.4105707605965421 1101 50-51 0.4392388300238892 1101 52-53 0.19917720319896404 1101 54-55 0.049371923961643915 1101 56-57 0.04836365466952941 1101 58-59 0.20275595528883272 1101 60-61 0.0034183742850615317 1101 62-63 0.022948071263424907 1101 64-65 -0.0683169708845206 1101 66-67 -0.10357806083102616 1101 68-69 -8.54151939582162 1101 70-71 -6.434992973849226 1101 72-73 -1.7695240826147867 1101 74-75 -0.44605322740146747 1101 76-77 -0.3512658936021147 1101 78-79 -0.33501133714662146 1101 80-81 -0.3502400860264139 1101 82-83 -0.22519231674563045 1101 84-85 -0.19672040659960643 1101 86-87 -0.4781245941299552 1101 88-89 -0.32165892800343343 1101 90-91 -0.6544579926200242 1101 92-93 -0.7449683261563855 1101 94-95 -0.3543936462189521 1101 96-97 -0.7485401892710613 1101 98-99 -1.0237605656127364 1101 100-101 -1.332252598057341 1102 1 0.2255495692731877 1102 2 0.11353319368168968 1102 3 0.2724568098097855 1102 4 0.13404821625680796 1102 5 0.31121623488789396 1102 6 0.21381529237162766 1102 7 0.4424426344164729 1102 8 0.22739876955426297 1102 9 0.2508616744576173 1102 10-11 0.4128591015985563 1102 12-13 0.27934201437955863 1102 14-15 0.46306873279128524 1102 16-17 0.3188441259499726 1102 18-19 0.28315719587153154 1102 20-21 0.1384591467517069 1102 22-23 0.20077006249204743 1102 24-25 0.17349087700045374 1102 26-27 0.39511208976555423 1102 28-29 0.11373849085823196 1102 30-31 0.04319483065774676 1102 32-33 -0.025866385642203227 1102 34-35 0.13833302429171823 1102 36-37 0.15571837386410436 1102 38-39 0.5762563431666621 1102 40-41 0.4689644747855013 1102 42-43 0.4772171587476919 1102 44-45 0.5014031228765603 1102 46-47 0.44255742871850856 1102 48-49 0.4034849006645089 1102 50-51 0.2533205918683663 1102 52-53 0.2406078688883042 1102 54-55 -0.026289434071273377 1102 56-57 0.21221302496338978 1102 58-59 0.09645722802821766 1102 60-61 0.0952030414403886 1102 62-63 0.1936309192676937 1102 64-65 -0.008928852006658161 1102 66-67 0.3278814247423725 1102 68-69 -3.746957691283736 1102 70-71 0.37151149073396894 1102 72-73 0.6087110743119766 1102 74-75 0.32483202614687556 1102 76-77 0.35620145496855216 1102 78-79 0.2994584103045703 1102 80-81 0.18993949683491707 1102 82-83 0.15759155078285758 1102 84-85 0.20249246072897265 1102 86-87 -0.26945114174106877 1102 88-89 -0.17815506314036966 1102 90-91 -0.15762401514284718 1102 92-93 -0.2945901692962529 1102 94-95 -0.07829582520209044 1102 96-97 -0.6497146411934978 1102 98-99 -0.28934995722998025 1102 100-101 -0.19368642893626387 1103 1 0.21869189376595344 1103 2 0.24949261507594755 1103 3 0.1872203053940389 1103 4 0.16387458010347444 1103 5 0.336389054570283 1103 6 0.17132682502365526 1103 7 0.17018835814992173 1103 8 0.3116694568867189 1103 9 0.3691519843083384 1103 10-11 0.3762526269839981 1103 12-13 0.26575950999103526 1103 14-15 0.3412419130816744 1103 16-17 0.25222009721019134 1103 18-19 0.2830398281738482 1103 20-21 0.268254382643768 1103 22-23 0.1780822061365015 1103 24-25 0.24929101852207936 1103 26-27 0.2522244021473341 1103 28-29 0.2546225928082393 1103 30-31 0.22259373794747006 1103 32-33 0.386062594993831 1103 34-35 0.2891641236698348 1103 36-37 0.24455364404794722 1103 38-39 0.44240409719117935 1103 40-41 0.4282093072048454 1103 42-43 0.39372177861242363 1103 44-45 0.36739676801106924 1103 46-47 0.30929032025034076 1103 48-49 0.39618207839662745 1103 50-51 0.38786241559228785 1103 52-53 0.30029801816640855 1103 54-55 0.2931881606053395 1103 56-57 0.21363113292806446 1103 58-59 0.17897556460475528 1103 60-61 0.20996738751520283 1103 62-63 0.3351465955097481 1103 64-65 0.27531819168813954 1103 66-67 0.4643557331234476 1103 68-69 -3.4901148143122995 1103 70-71 0.4633044268995512 1103 72-73 0.6763465064292262 1103 74-75 0.17267620266789407 1103 76-77 0.06733862889046804 1103 78-79 0.15506161223143877 1103 80-81 0.18783878514441454 1103 82-83 0.04085746856423356 1103 84-85 0.035140582165418266 1103 86-87 0.046710050034345585 1103 88-89 0.047697638698050326 1103 90-91 -0.1400996570931241 1103 92-93 -0.1431446755354493 1103 94-95 -0.353996289229741 1103 96-97 -0.6597800269079777 1103 98-99 -0.2830536054942989 1103 100-101 -0.44404856781797264 1104 1 0.13230049613533623 1104 2 0.20029086135947693 1104 3 0.14709954658093238 1104 4 0.15044525029792055 1104 5 0.151632480253582 1104 6 0.14193460480842646 1104 7 0.09195055487484893 1104 8 0.10022654171469725 1104 9 0.2930861436183889 1104 10-11 0.2889224652655571 1104 12-13 0.14756665206072483 1104 14-15 0.26204160100834173 1104 16-17 0.2769364751242236 1104 18-19 0.11251176277412611 1104 20-21 0.30282803788753654 1104 22-23 0.2454271234964267 1104 24-25 0.206549522096509 1104 26-27 0.15197508268769866 1104 28-29 0.18233640120539008 1104 30-31 0.11418488795467852 1104 32-33 0.07508575456676425 1104 34-35 0.2626623131356638 1104 36-37 0.2908911072648479 1104 38-39 0.36963012139382556 1104 40-41 0.3077393315431749 1104 42-43 0.2780850387611622 1104 44-45 0.21674353406631752 1104 46-47 0.1750616417282771 1104 48-49 0.1731503876884446 1104 50-51 0.23099332526910388 1104 52-53 0.2551242153662301 1104 54-55 0.2702356754669779 1104 56-57 0.19320813787204827 1104 58-59 0.15639824589137774 1104 60-61 0.33443037544780196 1104 62-63 0.36605508509175877 1104 64-65 0.2602732039421092 1104 66-67 0.2740383172263634 1104 68-69 -3.800698001361255 1104 70-71 0.7914129063052506 1104 72-73 0.7380420483598371 1104 74-75 0.48602683571883176 1104 76-77 0.47034284357657086 1104 78-79 0.08738308168980424 1104 80-81 0.16395533775166626 1104 82-83 0.035219626279992866 1104 84-85 -0.038507707791417545 1104 86-87 0.12021434528286079 1104 88-89 -0.1263190334807831 1104 90-91 0.009161458399454148 1104 92-93 -0.06895653262621337 1104 94-95 0.26022119535739563 1104 96-97 -0.14715818686251936 1104 98-99 0.18907943272620287 1104 100-101 0.15236598090532638 1105 1 0.12167135983643362 1105 2 0.2547551321142727 1105 3 0.09414319238808844 1105 4 0.12512900668562565 1105 5 0.2785203080159988 1105 6 0.2375214356704518 1105 7 0.14926478793172748 1105 8 0.2460437487181295 1105 9 0.24836133839094288 1105 10-11 0.20645367076107846 1105 12-13 0.21279577160499485 1105 14-15 0.33847705380217263 1105 16-17 0.33469303037261255 1105 18-19 0.38562728593740303 1105 20-21 0.2561312128896276 1105 22-23 0.26005726507303706 1105 24-25 0.34461938889720756 1105 26-27 0.33307586177792103 1105 28-29 0.31816784964301803 1105 30-31 0.5289842512787928 1105 32-33 0.46707285350284167 1105 34-35 0.5510128199631765 1105 36-37 0.4640108190852388 1105 38-39 0.6428109876081152 1105 40-41 0.541954796065248 1105 42-43 0.5942658212023204 1105 44-45 0.5687004746711537 1105 46-47 0.551886639633949 1105 48-49 0.6742050915503839 1105 50-51 0.6362517654131921 1105 52-53 0.5689786176448592 1105 54-55 0.46735890575935457 1105 56-57 0.6604402732494492 1105 58-59 0.6795765989114066 1105 60-61 0.528344256657487 1105 62-63 0.6640721748127909 1105 64-65 0.707976982110825 1105 66-67 1.0465180022393454 1105 68-69 2.257144002492307 1105 70-71 2.4126443714975103 1105 72-73 1.6224057913438372 1105 74-75 1.1714343920562733 1105 76-77 0.7806452646203859 1105 78-79 0.702256165379179 1105 80-81 0.6707803356573372 1105 82-83 0.6913007186817701 1105 84-85 0.5817117778414875 1105 86-87 0.38363899134133206 1105 88-89 0.7110555162385772 1105 90-91 0.6267169581062433 1105 92-93 0.6952572564330133 1105 94-95 0.815856614641973 1105 96-97 0.058562681027233054 1105 98-99 0.2948011383473812 1105 100-101 0.3131810935802015 1106 1 0.37981515916935393 1106 2 0.3731269759392646 1106 3 0.43464486008605974 1106 4 0.2979440204950734 1106 5 0.45730130380525225 1106 6 0.39004335722073336 1106 7 0.3815524771513097 1106 8 0.4079410004122437 1106 9 0.539060715079394 1106 10-11 0.42025481475661053 1106 12-13 0.5075078107242135 1106 14-15 0.6320111397376706 1106 16-17 0.4813312229200406 1106 18-19 0.7369188085984817 1106 20-21 0.5025803745129593 1106 22-23 0.4684874405963626 1106 24-25 0.569258683181765 1106 26-27 0.8407167105682731 1106 28-29 0.7097172995290748 1106 30-31 0.8954042847836732 1106 32-33 0.9307551457197505 1106 34-35 0.6914024274127257 1106 36-37 0.8656377678770966 1106 38-39 0.9451053928964512 1106 40-41 0.6641751736743302 1106 42-43 0.7340770166625532 1106 44-45 0.7668214846471599 1106 46-47 1.008208045027935 1106 48-49 1.1666680022493097 1106 50-51 1.1372935106855309 1106 52-53 0.9529249494639345 1106 54-55 1.0045730955297927 1106 56-57 1.1110752813238207 1106 58-59 1.0140326728032498 1106 60-61 1.2751737439724131 1106 62-63 1.1705686507632258 1106 64-65 1.2151277088045624 1106 66-67 1.7250511071760357 1106 68-69 3.2583272379773014 1106 70-71 3.338478975098532 1106 72-73 2.2626229693875786 1106 74-75 1.8401744877413861 1106 76-77 1.4121998458848175 1106 78-79 1.4403890982637684 1106 80-81 1.625143516508242 1106 82-83 1.6842514876339116 1106 84-85 1.4949016841183713 1106 86-87 1.476369035561742 1106 88-89 1.5516198169373503 1106 90-91 1.4037298921982178 1106 92-93 1.2802808004043946 1106 94-95 1.4972825341397034 1106 96-97 1.460721633018661 1106 98-99 1.6563222147294603 1106 100-101 1.7186769507467758 1107 1 0.1907745395369176 1107 2 0.09689797226610608 1107 3 -0.0014309853312397536 1107 4 0.05486975762098467 1107 5 0.13583205595074332 1107 6 0.056012768548427516 1107 7 0.05318665640080411 1107 8 0.05174559700186876 1107 9 0.012071906468882787 1107 10-11 0.055013511453225306 1107 12-13 4.3989797745069836E-4 1107 14-15 -0.03761742309512783 1107 16-17 0.04171576096630503 1107 18-19 0.06821352388652002 1107 20-21 0.23014751747754758 1107 22-23 0.15025446291313926 1107 24-25 0.24186276494598502 1107 26-27 0.3278946185106122 1107 28-29 0.21587881870915737 1107 30-31 0.3713313235552178 1107 32-33 0.2878659605836873 1107 34-35 0.32494179068083895 1107 36-37 0.0441032842451321 1107 38-39 0.2060828474117642 1107 40-41 0.19579035012559842 1107 42-43 0.11755287098262102 1107 44-45 0.20818948657506553 1107 46-47 0.03240744854268485 1107 48-49 -0.05539650288743303 1107 50-51 -0.08844587929087311 1107 52-53 -0.1340274345583694 1107 54-55 -0.28232828946413235 1107 56-57 -0.18944656652178082 1107 58-59 0.04359810275306941 1107 60-61 0.14156684248765217 1107 62-63 0.10812803450770403 1107 64-65 -0.2505628771355184 1107 66-67 0.3261421947408678 1107 68-69 1.6656041738298093 1107 70-71 1.7851776993937172 1107 72-73 0.7893903600946253 1107 74-75 0.24380205745540806 1107 76-77 0.005703628792428361 1107 78-79 0.06915492945199375 1107 80-81 -0.07018621581017825 1107 82-83 0.0958913539082431 1107 84-85 0.09688170508592009 1107 86-87 0.0842482895100396 1107 88-89 0.11609233579049061 1107 90-91 0.22586876612125195 1107 92-93 0.21150713324807668 1107 94-95 0.4675718586500892 1107 96-97 0.3145666297415204 1107 98-99 0.5281076258606738 1107 100-101 0.7617671004513724 1108 1 -0.10179857373271517 1108 2 -0.11865354703333963 1108 3 -0.07729154145713579 1108 4 -0.11345265149744677 1108 5 0.04705969202083082 1108 6 0.1110974860543763 1108 7 0.05879459554108024 1108 8 0.022830911171759283 1108 9 0.08576622148989799 1108 10-11 0.03412255178946566 1108 12-13 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66-67 -0.5550070170547272 2316 68-69 0.7177443237976924 2316 70-71 0.9764644404836673 2316 72-73 0.1538507992977216 2316 74-75 -0.22923932547849546 2316 76-77 -0.602894134664556 2316 78-79 -0.7270204250037118 2316 80-81 -0.8535463405644883 2316 82-83 -0.6283654544766328 2316 84-85 -0.7638872046914713 2316 86-87 -0.5782504890574636 2316 88-89 -0.48382564802092176 2316 90-91 -0.6310770599435571 2316 92-93 -0.8425386516484501 2316 94-95 -0.30525070765567364 2316 96-97 -0.3457351722118105 2316 98-99 -0.5389079700301842 2316 100-101 -0.5370083033421942 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4.0 3 22.0 4 55.0 5 142.0 6 326.0 7 772.0 8 1650.0 9 2744.0 10 4572.0 11 7033.0 12 9399.0 13 12019.0 14 14756.0 15 18219.0 16 22453.0 17 25974.0 18 22227.0 19 16586.0 20 15978.0 21 16571.0 22 17126.0 23 18875.0 24 22012.0 25 25648.0 26 30990.0 27 37717.0 28 45545.0 29 53860.0 30 63848.0 31 75313.0 32 89450.0 33 108307.0 34 135109.0 35 169977.0 36 203983.0 37 206081.0 38 105071.0 39 7099.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.53972048535818 19.12536718885625 14.413776496553199 21.921135829232373 2 16.272215121900835 21.867233807430157 38.26184132420234 23.598709746466675 3 19.443350620928875 27.074713641150822 29.718490262275033 23.763445475645266 4 13.257124514700658 17.066051720888105 35.558903722707065 34.117920041704174 5 13.659111932531806 38.74898678890933 31.7930865877912 15.798814690767665 6 30.945192978221638 35.65134465475551 17.43394921223032 15.969513154792528 7 28.202633509029162 34.39275452204741 19.586902252112427 17.817709716811 8 27.614955524465433 32.960168906876646 19.565502736214263 19.859372832443654 9 28.66378063505552 15.806341846069053 19.307339971745176 36.22253754713026 10-11 25.62592028804744 26.712661110672197 25.819666777189358 21.841751824091002 12-13 27.35386898892886 24.3459617433887 26.785133308408703 21.515035959273735 14-15 23.982356596805126 25.03009306923179 26.432041296089054 24.55550903787403 16-17 22.0165517215638 28.64377220247632 26.55637784357326 22.783298232386617 18-19 21.98455004718469 26.18551140799483 30.4804066903347 21.349531854485782 20-21 23.54316885773241 25.165612968604297 30.032603157797165 21.258615015866123 22-23 22.85664252792979 25.621130280128373 29.73739559182414 21.784831600117695 24-25 22.203487254519565 25.88162693754506 30.249528619088768 21.66535718884661 26-27 22.09856302559954 26.11178969704378 30.23761290368735 21.552034373669333 28-29 21.808259655834714 25.98185084097324 30.462469187642398 21.747420315549647 30-31 21.854046533588843 26.29312932366328 30.16596210712809 21.686862035619786 32-33 21.47226268449474 26.47809640586153 29.7728192442197 22.276821665424027 34-35 21.94343481472841 26.230071153621836 30.014697305493993 21.81179672615576 36-37 22.362603420295848 26.01580121383014 29.651936137539188 21.969659228334827 38-39 22.069527217659708 25.821735397061325 29.85190798531222 22.256829399966747 40-41 21.790007211367808 26.007193930992567 30.084107714668967 22.118691142970658 42-43 21.763312024959756 26.110251372381732 29.681682167911642 22.44475443474687 44-45 21.597124837784857 26.252014456762968 29.52340506086337 22.6274556445888 46-47 21.808108224226793 26.4405830887815 28.73404653427555 23.01726215271616 48-49 21.710566052069044 26.369363810202223 28.520196041925722 23.399874095803007 50-51 21.938957544752576 26.81770264826148 28.153508381397973 23.089831425587967 52-53 21.851014649020524 26.62873937244097 28.173295590726376 23.346950387812136 54-55 21.371769043382564 26.58692650121272 28.323704279370897 23.71760017603382 56-57 21.574640472441924 26.912045651132253 27.674443206780463 23.838870669645356 58-59 21.474604748277933 26.368717447319213 27.903279944577815 24.25339785982504 60-61 21.652980689489556 26.55781550278313 27.966240357654122 23.822963450073196 62-63 21.720060529806887 26.431525267150757 27.826043680374003 24.02237052266835 64-65 21.45370028785097 26.43021869084109 27.899552639650025 24.216528381657916 66-67 21.127562713338758 26.584081174346135 27.89093879646719 24.397417315847918 68-69 21.009600259431007 26.434188907887307 28.107556396720746 24.448654435960936 70-71 20.913995993130722 26.580992296740718 27.695281777115866 24.80972993301269 72-73 20.976140025225334 26.482081878555007 27.30266879298303 25.239109303236628 74-75 21.03710935798496 26.72466792861881 26.984183284236675 25.25403942915956 76-77 20.80553806251198 26.55399418041703 27.29902152349613 25.34144623357486 78-79 20.853846117955186 26.525188460479676 27.228452827723714 25.39251259384142 80-81 20.740767467619378 26.896297095798616 27.084168620292054 25.278766816289956 82-83 20.691758444104927 26.594451549972874 26.70832317270415 26.005466833218044 84-85 20.66853228898297 26.098028219481538 26.752115776825786 26.48132371470971 86-87 20.75422752984605 26.139114238951315 26.868197118629624 26.23846111257301 88-89 20.651641763747513 26.07711683107217 26.828061304788964 26.443180100391352 90-91 20.60188454004072 26.080481442935337 26.826484089278953 26.49114992774499 92-93 20.374177241491072 25.858622241673036 26.85695907273121 26.910241444104678 94-95 20.119811442042522 25.56944521835984 27.22843274194437 27.08231059765327 96-97 20.277899622643858 25.38900658584463 27.15063447473456 27.182459316776946 98-99 20.27195566479935 25.258701333770528 27.07159392002479 27.39774908140533 100-101 20.176800763494608 25.29539377333816 27.275925656330376 27.25187980683686 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1615.0 1 1139.0 2 856.0 3 1146.5 4 1059.0 5 1103.0 6 1975.5 7 3727.5 8 5254.0 9 5338.0 10 4336.0 11 3518.0 12 3402.5 13 3412.5 14 3484.5 15 3543.5 16 3484.0 17 3624.0 18 3924.5 19 4069.0 20 4241.5 21 4697.0 22 5230.5 23 5747.5 24 6319.0 25 7157.0 26 8198.5 27 9514.0 28 11127.0 29 12880.0 30 15124.5 31 17885.0 32 20745.5 33 22301.5 34 23085.0 35 24645.5 36 26930.5 37 29925.5 38 32454.0 39 34157.5 40 36315.5 41 39564.5 42 42659.5 43 45797.5 44 48882.5 45 51889.0 46 55188.5 47 59004.5 48 66004.0 49 68029.5 50 64773.5 51 62492.5 52 60668.0 53 60483.5 54 59569.5 55 56793.0 56 53019.0 57 48808.0 58 45338.5 59 41353.5 60 36039.0 61 30644.5 62 24651.5 63 17906.0 64 12721.0 65 9785.5 66 7091.0 67 5322.0 68 4039.0 69 2696.0 70 1975.0 71 1546.0 72 1259.5 73 1071.5 74 824.0 75 628.5 76 430.5 77 227.0 78 141.0 79 94.0 80 67.5 81 46.5 82 31.5 83 23.5 84 17.0 85 13.0 86 7.0 87 3.5 88 3.0 89 1.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.5 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02301692116953331 2 0.024074455385430786 3 1.2441579010558546E-4 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 4.0435131784315274E-4 18-19 0.0 20-21 0.0 22-23 0.0 24-25 1.2441579010558546E-4 26-27 2.1772763268477455E-4 28-29 3.1103947526396364E-5 30-31 4.6655921289594546E-4 32-33 0.0030170829100604475 34-35 0.004696696076485851 36-37 0.10494471895406132 38-39 0.09654665312193432 40-41 0.10668654001553954 42-43 0.10749524265122584 44-45 0.10083899788057701 46-47 0.0941827531099282 48-49 0.09517807943077287 50-51 0.09430716890003378 52-53 0.07427622669303452 54-55 0.06242562268547751 56-57 0.03377888701366646 58-59 0.013343593488824041 60-61 0.00933118425791891 62-63 0.0025194197496381057 64-65 0.005225463184434589 66-67 0.1661572876860094 68-69 0.44089845618666845 70-71 0.4206497863469844 72-73 0.19741675495003772 74-75 0.02979758173028772 76-77 0.03265914490271619 78-79 0.005007735551749814 80-81 0.001959548694162971 82-83 6.220789505279273E-5 84-85 4.0435131784315274E-4 86-87 8.709105307390982E-4 88-89 2.488315802111709E-4 90-91 7.464947406335127E-4 92-93 0.0024572118545853126 94-95 0.08226994120731838 96-97 0.017293794824676377 98-99 0.018817888253469802 100-101 0.010388718473816385 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1607513.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 43.64107141673235 #Duplication Level Percentage of deduplicated Percentage of total 1 69.55434719855641 30.35426233436398 2 13.771138689861417 12.019744941079361 3 5.928124962039117 7.761291745069886 4 3.1954136755319826 5.57805105679578 5 1.927640876904884 4.2062156587409305 6 1.3137518459763464 3.440012288047062 7 0.868467628791755 2.6530600467854826 8 0.611885594298423 2.136267433571776 9 0.46067275272845204 1.8093827251408554 >10 2.174357426698558 16.309430186581046 >50 0.12122773189343959 3.6423381360109226 >100 0.06570310767035815 5.343989056850662 >500 0.004703152913961956 1.3164651155115439 >1k 0.0021377967790736164 1.5481495412547408 >5k 2.8503957054314886E-4 1.059031065001184 >10k+ 1.4251978527157443E-4 0.8223086691948467 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13221 0.8224505804929727 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9401 0.5848164213913045 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7626 0.47439740767259736 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2910 0.18102497460362685 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 2571 0.1599364981807301 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2138 0.13300047962287084 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 1994 0.1240425427352687 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1893 0.11775954533493663 No Hit CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGT 1789 0.11128992424944618 No Hit GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG 1722 0.10712199528090907 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 6.220789505279273E-5 0.0 3 0.0 0.0 0.0 6.220789505279273E-5 0.0 4 0.0 0.0 0.0 1.2441579010558546E-4 0.0 5 0.0 0.0 0.0 1.2441579010558546E-4 0.0 6 0.0 0.0 0.0 1.2441579010558546E-4 0.0 7 0.0 0.0 0.0 1.2441579010558546E-4 0.0 8 0.0 0.0 0.0 1.2441579010558546E-4 0.0 9 0.0 0.0 0.0 1.2441579010558546E-4 0.0 10-11 0.0 0.0 0.0 1.2441579010558546E-4 0.0 12-13 0.0 0.0 0.0 1.2441579010558546E-4 0.0 14-15 0.0 0.0 0.0 1.8662368515837819E-4 0.0 16-17 0.0 0.0 0.0 2.488315802111709E-4 0.0 18-19 0.0 0.0 0.0 4.354552653695491E-4 0.0 20-21 0.0 0.0 0.0 8.398065832127019E-4 0.0 22-23 0.0 0.0 0.0 0.00273714738232288 0.0 24-25 0.0 0.0 0.0 0.006438517137964048 0.0 26-27 0.0 0.0 0.0 0.013188073751192058 0.0 28-29 0.0 0.0 0.0 0.03272135279776898 0.0 30-31 0.0 0.0 0.0 0.09520918337829927 0.0 32-33 0.0 0.0 0.0 0.2201226366443071 0.0 34-35 0.0 0.0 0.0 0.3963887072763953 0.0 36-37 0.0 0.0 0.0 0.6702278612987889 0.0 38-39 0.0 0.0 0.0 1.0842525068226507 0.0 40-41 0.0 0.0 0.0 1.5713092211384916 0.0 42-43 0.0 0.0 0.0 2.017775283932385 0.0 44-45 0.0 0.0 0.0 2.5140076627685124 0.0 46-47 0.0 0.0 0.0 3.089741731482109 0.0 48-49 0.0 0.0 0.0 3.6978550095706844 0.0 50-51 0.0 0.0 0.0 4.3518466102606945 0.0 52-53 0.0 0.0 0.0 4.946865126440657 0.0 54-55 0.0 0.0 0.0 5.544060918947467 0.0 56-57 0.0 0.0 0.0 6.190711988021247 0.0 58-59 0.0 0.0 0.0 6.859229132206085 0.0 60-61 0.0 0.0 0.0 7.585879554317756 0.0 62-63 0.0 0.0 0.0 8.252063902438113 0.0 64-65 0.0 0.0 0.0 8.895884512286992 0.0 66-67 0.0 0.0 0.0 9.555381511689173 0.0 68-69 0.0 0.0 0.0 10.27898374694326 0.0 70-71 0.0 0.0 0.0 11.106846414305824 0.0 72-73 0.0 0.0 0.0 11.956077493619025 0.0 74-75 0.0 0.0 0.0 12.79725265052289 0.0 76-77 0.0 0.0 0.0 13.659827323324912 0.0 78-79 0.0 0.0 0.0 14.587035999086787 0.0 80-81 0.0 0.0 0.0 15.565752811952375 0.0 82-83 0.0 0.0 0.0 16.549228528789502 0.0 84-85 0.0 0.0 0.0 17.50138257046755 0.0 86-87 0.0 0.0 0.0 18.439384316021084 0.0 88-89 0.0 0.0 0.0 19.401398309064994 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 9335 0.0 33.35426 1 GTATCAA 14230 0.0 32.954433 1 GTACATG 14325 0.0 26.175459 1 TACATGG 14385 0.0 25.430662 2 TATCAAC 18445 0.0 25.389355 2 ATCAACG 18440 0.0 25.337683 3 TCAACGC 18510 0.0 25.19061 4 CAACGCA 18770 0.0 24.8164 5 AACGCAG 19210 0.0 24.27268 6 ACATGGG 14385 0.0 24.236452 3 CATGGGA 9970 0.0 22.31358 4 AGTACTT 10190 0.0 22.274057 12-13 CGTCACT 395 0.0 21.62904 1 ACGCAGA 21545 0.0 21.598028 7 CGCAGAG 21685 0.0 21.349216 8 GAGTACT 10105 0.0 21.240944 12-13 ATGGGAG 3175 0.0 21.065266 5 CATGGGG 4305 0.0 20.934954 4 GTCGGGA 870 0.0 20.724186 2 GCAGAGT 22150 0.0 20.322824 9 >>END_MODULE