FastQCFastQC Report
Fri 27 May 2016
SRR939765_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939765_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3612779
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT120210.3327355478981693No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76760.2124680197709298No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59360.1643056494737154No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA63550.046.05641
GTATCAA125250.045.2595861
TATCAAC164450.035.365762
ATCAACG166250.034.7850463
TCAACGC167200.034.5590254
CAACGCA169550.034.0250175
AACGCAG176250.032.839716
ACGCAGA203050.028.1080847
GTACATG158350.027.5906521
TACATGG164500.027.2227822
CGCAGAG209950.027.001668
ACATGGG168700.025.3654123
GCAGAGT212000.025.2860539
GAGTACT108100.021.76513312-13
CATGGGA92600.021.6710454
AGAGTAC187550.021.4987510-11
AGTACTT113950.021.06403212-13
CATGGGG82850.020.8429584
CAGAGTA203350.019.87498310-11
GTACTTT119700.019.79720514-15
Produced by FastQC (version 0.11.5)