##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939765_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3612779 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.86715185180162 31.0 30.0 33.0 16.0 34.0 2 29.71554529075817 31.0 30.0 33.0 25.0 34.0 3 29.989623500358036 31.0 30.0 34.0 25.0 34.0 4 33.77866705934684 35.0 35.0 37.0 28.0 37.0 5 33.726527971957324 35.0 35.0 37.0 28.0 37.0 6 33.75684729124035 35.0 35.0 37.0 28.0 37.0 7 33.671395897728594 35.0 33.0 37.0 28.0 37.0 8 33.54018831486786 35.0 33.0 37.0 28.0 37.0 9 34.812047180300816 37.0 34.0 39.0 27.0 39.0 10-11 34.648206131623326 37.0 34.0 39.0 27.0 39.0 12-13 34.51739436594378 37.0 33.5 39.0 27.0 39.0 14-15 35.16817926034224 38.0 33.5 40.0 26.5 41.0 16-17 35.0015191906286 38.0 33.0 40.0 26.5 41.0 18-19 34.83659947093359 38.0 33.0 40.0 25.0 41.0 20-21 35.01901721638661 38.0 34.0 40.0 25.5 41.0 22-23 34.90948699048572 38.0 33.5 40.0 25.0 41.0 24-25 34.68081164112169 38.0 33.0 40.0 24.5 41.0 26-27 34.557621708939294 38.0 33.0 40.0 24.0 41.0 28-29 34.61217029881983 38.0 33.0 40.0 24.0 41.0 30-31 34.38411829231735 38.0 33.0 40.0 24.0 41.0 32-33 34.03048290526489 38.0 33.0 40.0 22.5 41.0 34-35 33.84699285508469 38.0 33.0 40.0 21.5 41.0 36-37 33.63848646706593 38.0 32.0 40.0 20.5 41.0 38-39 33.4107282787018 37.5 32.0 40.0 18.5 41.0 40-41 33.16148607484709 37.0 31.0 40.0 18.0 41.0 42-43 32.753491702647736 37.0 31.0 40.0 15.0 41.0 44-45 32.38872831689954 37.0 30.5 40.0 14.0 41.0 46-47 31.98071249306974 36.5 30.0 39.5 10.0 41.0 48-49 31.606852647228074 36.0 30.0 39.0 8.5 40.5 50-51 30.959137550345595 35.5 28.5 38.5 8.0 40.0 52-53 31.09442744767947 35.5 29.5 39.0 7.0 40.0 54-55 31.17385079463759 36.0 29.0 39.0 4.0 40.0 56-57 30.85743952231786 35.5 28.5 39.0 2.0 40.0 58-59 30.44144895107063 35.0 28.0 39.0 2.0 40.0 60-61 30.01982919519849 35.0 28.0 38.0 2.0 40.0 62-63 29.55529635219868 34.0 27.0 38.0 2.0 40.0 64-65 28.999418314820808 34.0 26.0 38.0 2.0 40.0 66-67 28.38880900270955 34.0 26.0 37.0 2.0 39.0 68-69 27.839892365406243 33.0 25.0 36.5 2.0 39.0 70-71 27.255109570776405 33.0 24.0 36.0 2.0 39.0 72-73 26.57573588088283 32.0 22.0 35.0 2.0 38.0 74-75 26.211627115857347 32.0 21.5 35.0 2.0 37.5 76-77 25.62856308121809 31.5 20.0 35.0 2.0 37.0 78-79 24.950103092384005 31.0 18.0 34.5 2.0 36.0 80-81 24.204894071848845 31.0 10.5 34.0 2.0 36.0 82-83 23.44404999586191 30.0 6.5 34.0 2.0 35.0 84-85 22.657583677274474 29.5 2.0 34.0 2.0 35.0 86-87 21.7565551338734 29.0 2.0 34.0 2.0 35.0 88-89 20.798508295137896 28.0 2.0 33.5 2.0 35.0 90-91 19.441683119836558 26.0 2.0 32.5 2.0 34.5 92-93 17.939064775343304 22.0 2.0 32.0 2.0 34.0 94-95 17.33661912339504 20.0 2.0 32.0 2.0 34.0 96-97 16.91317030463253 19.5 2.0 32.0 2.0 34.0 98-99 15.95531279383544 8.5 2.0 31.5 2.0 34.0 100-101 14.311006707025257 2.0 2.0 30.0 2.0 33.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.6264110469703432 1101 2 1.0388137591336992 1101 3 0.8519333812990304 1101 4 0.9158907774102261 1101 5 1.0040594188931564 1101 6 1.001479010172723 1101 7 0.9604189313112812 1101 8 1.043103674607238 1101 9 1.055989688844214 1101 10-11 1.183489623528608 1101 12-13 1.1510175735249675 1101 14-15 1.2688459032705595 1101 16-17 1.1362332971972222 1101 18-19 1.0665814290644633 1101 20-21 1.0337121166970462 1101 22-23 1.2522305677556815 1101 24-25 1.4226317183168007 1101 26-27 1.2907477061296575 1101 28-29 1.2433364690148778 1101 30-31 1.3762545508607857 1101 32-33 1.6146503756260842 1101 34-35 1.2890384228522578 1101 36-37 1.2441840109696543 1101 38-39 1.2271012043569485 1101 40-41 1.087512594396749 1101 42-43 1.1877365326764604 1101 44-45 1.1127581180847486 1101 46-47 1.1422814208192804 1101 48-49 1.1100675534769557 1101 50-51 1.0157797335266459 1101 52-53 1.117040506620203 1101 54-55 1.1552145036613446 1101 56-57 1.19234864329297 1101 58-59 0.9323726614758279 1101 60-61 0.8558577831216461 1101 62-63 0.44401850620841543 1101 64-65 0.5014551498304307 1101 66-67 0.6816929825863092 1101 68-69 0.40165967775737954 1101 70-71 0.39417719412432106 1101 72-73 0.5738758855504713 1101 74-75 0.5641150962272512 1101 76-77 0.39992800929777417 1101 78-79 0.14266706112404748 1101 80-81 0.14229721702954023 1101 82-83 0.11055368451403425 1101 84-85 0.27019784766740074 1101 86-87 0.017581631013602106 1101 88-89 -4.143005180185973 1101 90-91 -7.6410164006924575 1101 92-93 -9.991958890810633 1101 94-95 -7.990229729674464 1101 96-97 -4.687545991502709 1101 98-99 -3.261187351679226 1101 100-101 -2.4254511403187156 1102 1 1.7924524709803968 1102 2 1.2546916602824503 1102 3 1.2229413842491539 1102 4 1.2398975234171843 1102 5 1.210704482927781 1102 6 1.1294978198946382 1102 7 0.9811406231173692 1102 8 1.2083471083902495 1102 9 1.496942587379209 1102 10-11 1.4906026258257157 1102 12-13 1.5651349191736514 1102 14-15 1.6427086669889661 1102 16-17 1.6115298379145955 1102 18-19 1.6887242048479223 1102 20-21 1.5870106154800112 1102 22-23 1.4506148129100396 1102 24-25 1.607570379289868 1102 26-27 1.6393520248968585 1102 28-29 1.4053047691954887 1102 30-31 1.7129406233760207 1102 32-33 1.7141736858592296 1102 34-35 1.8551296502707828 1102 36-37 1.68655451960241 1102 38-39 1.6321961498287365 1102 40-41 1.5922009957474188 1102 42-43 1.6317145456908193 1102 44-45 1.7422591721065857 1102 46-47 1.7833693664236634 1102 48-49 1.600066287515066 1102 50-51 1.5610941547031452 1102 52-53 1.5764069711894741 1102 54-55 1.6953638671230173 1102 56-57 1.636354547552184 1102 58-59 1.4417599860240315 1102 60-61 1.463657611498629 1102 62-63 1.402633410287585 1102 64-65 1.3964242373124058 1102 66-67 1.4263209117098121 1102 68-69 1.3734018383969193 1102 70-71 1.2124289909384416 1102 72-73 1.1388980599252 1102 74-75 1.227432530994303 1102 76-77 1.1530786343789856 1102 78-79 0.9084457395867638 1102 80-81 0.7913071112398846 1102 82-83 0.4371658402200005 1102 84-85 0.5511937889067262 1102 86-87 0.7528205654544813 1102 88-89 0.34201939250178626 1102 90-91 -5.541951208331383 1102 92-93 -10.355089704757324 1102 94-95 -8.27139836281307 1102 96-97 -4.6599121143624735 1102 98-99 -3.0832842989741263 1102 100-101 -2.246480203663907 1103 1 2.1874822859424654 1103 2 1.3944031387726632 1103 3 1.1568937201768037 1103 4 1.0647210220599064 1103 5 1.108082966153276 1103 6 1.0433837461133848 1103 7 0.9454176658247277 1103 8 1.0254798862599515 1103 9 1.089857940457577 1103 10-11 1.0369383599025213 1103 12-13 1.0338178613264546 1103 14-15 1.2013847861277682 1103 16-17 1.1630653883152036 1103 18-19 1.0859594912379862 1103 20-21 0.9997077377820034 1103 22-23 1.0237956006859648 1103 24-25 0.9819611172825304 1103 26-27 1.0050025924366537 1103 28-29 0.8212414422112531 1103 30-31 1.120041601341363 1103 32-33 1.1570567010694361 1103 34-35 1.211116502819948 1103 36-37 1.1660831360339827 1103 38-39 1.082507208178832 1103 40-41 1.0206766681361188 1103 42-43 1.1442501227494475 1103 44-45 1.0542886789402814 1103 46-47 1.1181478370166218 1103 48-49 1.0887669464486613 1103 50-51 0.9638758117709223 1103 52-53 0.9501929201996298 1103 54-55 0.9329809733149084 1103 56-57 0.8903527112704133 1103 58-59 0.9206466483405578 1103 60-61 0.8186955823760549 1103 62-63 0.8675221466466603 1103 64-65 0.832500629611939 1103 66-67 0.8516757336546057 1103 68-69 0.8423021875690431 1103 70-71 0.7361516331380038 1103 72-73 0.8103010094557881 1103 74-75 0.7260907436240132 1103 76-77 0.7266376779466093 1103 78-79 0.5724247287383903 1103 80-81 0.3720959438800726 1103 82-83 0.15814095242987847 1103 84-85 0.11693830798030902 1103 86-87 -0.010485092363563098 1103 88-89 -0.8373023120076724 1103 90-91 -6.482846699498332 1103 92-93 -9.718289595051136 1103 94-95 -8.430766413863914 1103 96-97 -5.251828932191362 1103 98-99 -3.7686235127640586 1103 100-101 -3.0435367852066104 1104 1 1.9172314165752304 1104 2 1.2846705750470733 1104 3 1.0395144151038629 1104 4 0.8272077846793238 1104 5 0.7840265516827358 1104 6 0.8354814500085865 1104 7 0.7320623926519758 1104 8 0.7975110062131918 1104 9 0.9894587926582261 1104 10-11 0.9453485364909895 1104 12-13 0.7797098416649462 1104 14-15 0.9291330376732532 1104 16-17 1.047857804441108 1104 18-19 0.8507053565952916 1104 20-21 0.9302156907541246 1104 22-23 0.9463295928295352 1104 24-25 0.945239519742394 1104 26-27 0.7894975422977026 1104 28-29 0.7997712840612081 1104 30-31 1.0560916262839797 1104 32-33 1.1150607116580176 1104 34-35 1.0860803739143918 1104 36-37 0.9003939981655549 1104 38-39 0.8093176238474129 1104 40-41 0.763209006224912 1104 42-43 0.9015367282666134 1104 44-45 0.9113486591838864 1104 46-47 0.8028851289665155 1104 48-49 0.8372001414169041 1104 50-51 0.7651894343947028 1104 52-53 0.7995755162484777 1104 54-55 0.6601355114547083 1104 56-57 0.6533334497696295 1104 58-59 0.6250178669967141 1104 60-61 0.678493814053116 1104 62-63 0.7301285811174267 1104 64-65 0.800202969605202 1104 66-67 0.7514107883471475 1104 68-69 0.595670989723665 1104 70-71 0.601761857437058 1104 72-73 0.6241153322521562 1104 74-75 0.3991811835169159 1104 76-77 0.5664054710619197 1104 78-79 0.3326193734230394 1104 80-81 0.20194616295995615 1104 82-83 0.08327994904466962 1104 84-85 -0.09822644180601614 1104 86-87 0.03224988390964967 1104 88-89 -0.011961797484612902 1104 90-91 -2.854751063501091 1104 92-93 -7.5409209523711205 1104 94-95 -5.246194725087168 1104 96-97 -2.609188948745089 1104 98-99 -1.6776018011534504 1104 100-101 -1.1308460005322516 1105 1 1.9528138302899478 1105 2 1.3094207131007316 1105 3 0.9362085493522336 1105 4 0.8456590350510069 1105 5 0.9998699299852305 1105 6 0.8440289668399288 1105 7 0.8561785808493383 1105 8 0.7903496741202787 1105 9 0.8494385432327292 1105 10-11 0.9327860039030895 1105 12-13 0.7736829068482152 1105 14-15 1.0612201148708067 1105 16-17 0.8097978935889927 1105 18-19 0.8459482353372678 1105 20-21 0.8290406825969399 1105 22-23 0.8411285526308276 1105 24-25 0.9229828431034974 1105 26-27 0.8008076726586353 1105 28-29 0.8369508256997307 1105 30-31 1.21002717074753 1105 32-33 1.2593559331577069 1105 34-35 1.1990788433042923 1105 36-37 1.1448402267917501 1105 38-39 1.039737834435833 1105 40-41 0.8923888578131525 1105 42-43 0.9438472913434737 1105 44-45 0.9243335449342709 1105 46-47 1.1127810670848675 1105 48-49 0.9262106036200812 1105 50-51 0.9999150518784781 1105 52-53 0.8634837860709048 1105 54-55 0.7204021055508143 1105 56-57 0.7283868857535758 1105 58-59 0.6299917196576352 1105 60-61 0.5591675158936305 1105 62-63 0.6715592417724352 1105 64-65 0.924262629323362 1105 66-67 0.733778710792496 1105 68-69 0.7071713843960801 1105 70-71 0.45512702907871017 1105 72-73 0.3935069579150614 1105 74-75 0.5244873172070008 1105 76-77 0.29342655090991343 1105 78-79 0.08723409986207642 1105 80-81 0.008981881396781688 1105 82-83 -0.29709005809257505 1105 84-85 -0.20472119530030497 1105 86-87 0.039116110267233495 1105 88-89 -0.2049123553707517 1105 90-91 -3.3231288175139824 1105 92-93 -7.861982828883232 1105 94-95 -5.683371979446187 1105 96-97 -3.0939138893808504 1105 98-99 -2.175941142874409 1105 100-101 -1.5137814016907356 1106 1 1.4975750620256463 1106 2 0.9254371687441534 1106 3 0.6888246459906497 1106 4 0.7164513103494698 1106 5 0.6659789700049856 1106 6 0.6839552842161822 1106 7 0.5354472177314875 1106 8 -0.43394680222098714 1106 9 -0.6624969299472028 1106 10-11 -0.7393144266249436 1106 12-13 -0.3306785148672091 1106 14-15 0.5773915771800944 1106 16-17 0.6092673909926845 1106 18-19 0.7203715207355614 1106 20-21 0.63780469988221 1106 22-23 0.6829735769689762 1106 24-25 0.6410461778729868 1106 26-27 0.8426835940923425 1106 28-29 0.6411662078561733 1106 30-31 0.7601027162843934 1106 32-33 0.6053115274029324 1106 34-35 0.6840120991632475 1106 36-37 0.48666934821305574 1106 38-39 0.4525598484560618 1106 40-41 0.3856962021275976 1106 42-43 0.46060956705888856 1106 44-45 0.30977447802655433 1106 46-47 0.4351974043785205 1106 48-49 0.27631665870243793 1106 50-51 0.15469059089381076 1106 52-53 0.16854055280211 1106 54-55 0.1933982845743607 1106 56-57 0.1642902462670861 1106 58-59 0.050516090944505265 1106 60-61 -0.18430460402131033 1106 62-63 -0.017272393646109663 1106 64-65 0.01560557585855804 1106 66-67 -0.001781975272695746 1106 68-69 -0.20349364618563115 1106 70-71 -0.2542168299364498 1106 72-73 -0.03450006983780085 1106 74-75 -0.1522226205390922 1106 76-77 -0.25166844162239954 1106 78-79 -0.3131108731590935 1106 80-81 -0.537581324545723 1106 82-83 -0.5320560552509015 1106 84-85 -0.4022612813417652 1106 86-87 -0.26218549670246816 1106 88-89 -0.6368673156375131 1106 90-91 -3.9677534178028537 1106 92-93 -8.492863767620387 1106 94-95 -6.0468400928951915 1106 96-97 -3.400396974022856 1106 98-99 -2.2195760070929005 1106 100-101 -1.7079545462346175 1107 1 1.9236438444488506 1107 2 1.0603250628188867 1107 3 0.8714990318167928 1107 4 0.8899330734144684 1107 5 0.682281557129528 1107 6 0.5850773953165387 1107 7 0.7327443899441946 1107 8 0.7830477954893453 1107 9 0.7345461158670759 1107 10-11 0.7120076407185465 1107 12-13 0.5190537373069617 1107 14-15 0.8426594091537396 1107 16-17 0.8338802584556646 1107 18-19 0.9426913602656768 1107 20-21 0.9246559202358782 1107 22-23 0.7140985385765433 1107 24-25 0.6225415476395213 1107 26-27 0.6829050395834955 1107 28-29 0.40395273116301667 1107 30-31 0.6421224847096099 1107 32-33 0.8440759534937996 1107 34-35 0.700623403580579 1107 36-37 0.3119745418161415 1107 38-39 0.3747323241268248 1107 40-41 0.4722929805285432 1107 42-43 0.49274167281586045 1107 44-45 0.32750836687571194 1107 46-47 0.24507830366621164 1107 48-49 0.3891704476681319 1107 50-51 0.571944100909505 1107 52-53 0.6199096903290915 1107 54-55 0.4845697062937582 1107 56-57 0.35968778157761605 1107 58-59 0.30643667854868895 1107 60-61 0.035701170622427725 1107 62-63 -0.01714301638200766 1107 64-65 0.18231475470211578 1107 66-67 0.15660104033251443 1107 68-69 0.1813043149128326 1107 70-71 0.014944153246606362 1107 72-73 0.02292213550810729 1107 74-75 -0.2510886761523814 1107 76-77 -0.06630895164229855 1107 78-79 -0.1457561228046167 1107 80-81 -0.09196027160412612 1107 82-83 -0.5117738024456209 1107 84-85 -0.2973797088715635 1107 86-87 -0.04773742221482635 1107 88-89 -0.20433151074263733 1107 90-91 -3.4821647828066205 1107 92-93 -4.912510231098132 1107 94-95 -3.284654963496889 1107 96-97 -1.9892662693269862 1107 98-99 -1.4535208671340474 1107 100-101 -1.2217752987685184 1108 1 1.677165786830546 1108 2 0.9923089537544918 1108 3 -0.4511966670872454 1108 4 -0.2618730490563266 1108 5 0.37521971163599943 1108 6 0.5512773680903535 1108 7 0.578077379742929 1108 8 0.5282044565325918 1108 9 0.807833022138432 1108 10-11 0.6782676607746936 1108 12-13 0.5631096443559045 1108 14-15 0.6204433935991744 1108 16-17 0.5698752543966776 1108 18-19 0.6393828262817678 1108 20-21 0.5419489317300332 1108 22-23 0.5000987552456664 1108 24-25 0.5542975439932079 1108 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2.7394336267429864 2311 92-93 3.6171908515890614 2311 94-95 3.369422421617589 2311 96-97 2.6512802882940356 2311 98-99 2.272662215943333 2311 100-101 2.132572801306239 2312 1 1.3273761010110796 2312 2 0.6252270096806356 2312 3 -0.018320406449515048 2312 4 -0.6165584961249095 2312 5 -0.018221465111075474 2312 6 -0.24516547685274048 2312 7 0.21097663502030883 2312 8 0.28875830918323686 2312 9 0.24397517239756183 2312 10-11 0.1617391788764735 2312 12-13 0.22882579360702238 2312 14-15 0.21556075664910423 2312 16-17 0.04397392598622929 2312 18-19 -0.19661929529913635 2312 20-21 0.4171279898319966 2312 22-23 0.5533976906227736 2312 24-25 0.46205649942916693 2312 26-27 0.5080204924462137 2312 28-29 0.5239474823563626 2312 30-31 0.5133864516518756 2312 32-33 0.7026763042031945 2312 34-35 0.5432202889637665 2312 36-37 0.6080850298925071 2312 38-39 0.5243716949104638 2312 40-41 0.49510083800603155 2312 42-43 0.6722282364973253 2312 44-45 0.564194914963899 2312 46-47 0.6358218521240389 2312 48-49 0.7305423214433837 2312 50-51 0.6071775857899517 2312 52-53 0.5612403533146342 2312 54-55 0.6068245247191548 2312 56-57 0.662018088145512 2312 58-59 0.6444640043652292 2312 60-61 0.6566556215617503 2312 62-63 0.7934821798391276 2312 64-65 0.7402883985921633 2312 66-67 0.8000826350508525 2312 68-69 0.7491769888874522 2312 70-71 0.6797861808731511 2312 72-73 0.7370420345416733 2312 74-75 0.7711594072326378 2312 76-77 1.082669532427154 2312 78-79 1.0884642170869405 2312 80-81 1.0555641746780964 2312 82-83 1.0994392044314303 2312 84-85 1.4400373387512637 2312 86-87 1.9107743809869469 2312 88-89 2.301986374809708 2312 90-91 2.99001236511436 2312 92-93 3.9085828189667104 2312 94-95 3.5949566248101057 2312 96-97 2.706254215289622 2312 98-99 2.484119011127051 2312 100-101 2.4499139328938995 2313 1 -7.373558726481679 2313 2 -2.7977828964361606 2313 3 -1.6763124690768088 2313 4 -0.9627685240542547 2313 5 -0.8285414931242343 2313 6 -0.31775232184254776 2313 7 -0.3703713074372317 2313 8 -0.08280896105156899 2313 9 -0.1188442712320068 2313 10-11 -0.19652119963647152 2313 12-13 -0.3166947198868755 2313 14-15 -0.40926841009905246 2313 16-17 -0.5672450399854227 2313 18-19 -0.7711435698020352 2313 20-21 -0.5505856896799344 2313 22-23 -1.28136581096647 2313 24-25 -0.5217853524009612 2313 26-27 -0.5093084019000216 2313 28-29 -0.7519307577130832 2313 30-31 -0.32058184588738214 2313 32-33 -0.6530365343551381 2313 34-35 -0.45670392742672306 2313 36-37 -0.3886134938288066 2313 38-39 -0.3089793329061976 2313 40-41 -0.38832883713256194 2313 42-43 -0.43441839349519995 2313 44-45 -0.5061780242108469 2313 46-47 -1.3792817863273754 2313 48-49 -0.9174758731419175 2313 50-51 -0.7923423116565509 2313 52-53 -1.1240430052100585 2313 54-55 -0.8128727878613411 2313 56-57 -0.5096685150817741 2313 58-59 -0.3953432282082652 2313 60-61 -0.38643358843544817 2313 62-63 -0.5946099916558047 2313 64-65 -0.44076568738998745 2313 66-67 -0.36053333319593506 2313 68-69 -0.2172649578409782 2313 70-71 -0.1729788964563994 2313 72-73 -0.38053303430383423 2313 74-75 -0.13682567073018603 2313 76-77 -0.03663144700906429 2313 78-79 -0.07317779837059035 2313 80-81 0.07503765204452506 2313 82-83 0.2953494389205851 2313 84-85 0.2687645768938616 2313 86-87 0.8389780253986139 2313 88-89 1.356615356417688 2313 90-91 1.982152231157805 2313 92-93 2.963138112364529 2313 94-95 2.5855570708042173 2313 96-97 1.6023807881196497 2313 98-99 1.3519886581966123 2313 100-101 1.1685391693247915 2314 1 0.46233425063931577 2314 2 -0.1406357293348428 2314 3 -1.3291736402908363 2314 4 -0.39505881218423156 2314 5 -0.17892102066288373 2314 6 -3.676594406550951 2314 7 -3.017061570024758 2314 8 -2.8456595451038282 2314 9 -3.615737300178271 2314 10-11 -2.831342666217502 2314 12-13 -1.564060075991243 2314 14-15 -1.4235135334339333 2314 16-17 -2.295773762288981 2314 18-19 -3.398901414389165 2314 20-21 -3.303361696246938 2314 22-23 -4.38311257697724 2314 24-25 -3.3274490443589713 2314 26-27 -1.999677809629759 2314 28-29 -2.019186836539312 2314 30-31 -2.1424166408166556 2314 32-33 -2.2680056844392595 2314 34-35 -1.7927498353591318 2314 36-37 -2.146968351614724 2314 38-39 -3.2633918379730424 2314 40-41 -2.6796638837845563 2314 42-43 -1.797427553611211 2314 44-45 -2.418416792472314 2314 46-47 -1.9137816817513915 2314 48-49 -2.5960972099746904 2314 50-51 -3.3155362010873404 2314 52-53 -2.308266098179036 2314 54-55 -2.8429404661078728 2314 56-57 -1.8856493776763337 2314 58-59 -1.228496407040602 2314 60-61 -1.6221298146305294 2314 62-63 -1.3891295158998531 2314 64-65 -1.899152658332671 2314 66-67 -1.2409252432716933 2314 68-69 -1.1265901743502873 2314 70-71 -1.3612259669406122 2314 72-73 -2.334555461688307 2314 74-75 -2.5236737597659946 2314 76-77 -1.3997285249894311 2314 78-79 -2.116908838189051 2314 80-81 -1.1748124248604697 2314 82-83 -0.9196459477051668 2314 84-85 -2.085393914953837 2314 86-87 -0.797337582150849 2314 88-89 0.22158635740422383 2314 90-91 1.3605010920199199 2314 92-93 2.389233777180909 2314 94-95 1.9918550207447048 2314 96-97 1.2954404362260838 2314 98-99 1.0874862594270933 2314 100-101 1.1770269450398416 2315 1 -3.03566939267213 2315 2 -2.151174440491534 2315 3 -2.864887404976294 2315 4 -4.135389593935187 2315 5 -4.090462882515634 2315 6 -3.125547927097582 2315 7 -5.927849676840111 2315 8 -3.1637400373885356 2315 9 -2.840864964398392 2315 10-11 -4.223076514341209 2315 12-13 -3.1627920776582634 2315 14-15 -2.2692398153159488 2315 16-17 -2.773438967157162 2315 18-19 -2.3628075504900963 2315 20-21 -1.6344233699429793 2315 22-23 -1.0590915831974215 2315 24-25 -2.542428126440946 2315 26-27 -4.108823848938819 2315 28-29 -3.586736600176323 2315 30-31 -3.595507303931427 2315 32-33 -4.48180848315603 2315 34-35 -4.155058971210593 2315 36-37 -3.4326944559995844 2315 38-39 -2.250626084614332 2315 40-41 -1.8744038528854645 2315 42-43 -3.3749746620853145 2315 44-45 -6.713858495355694 2315 46-47 -3.539031211140788 2315 48-49 -2.547847830363718 2315 50-51 -3.51687669363465 2315 52-53 -3.5953969223347926 2315 54-55 -2.0358252252296474 2315 56-57 -1.7309854697531044 2315 58-59 -2.986716348870168 2315 60-61 -3.2599071782482802 2315 62-63 -5.240471455170535 2315 64-65 -4.575922134754972 2315 66-67 -4.033854814346171 2315 68-69 -2.4119190376731012 2315 70-71 -3.859495126542651 2315 72-73 -2.846942209993937 2315 74-75 -1.9306036133770412 2315 76-77 -3.4268052903202033 2315 78-79 -1.8770363881301897 2315 80-81 -1.55661111293767 2315 82-83 -1.203513073335703 2315 84-85 -2.7266462108673686 2315 86-87 -2.227780702579995 2315 88-89 -1.6395174998277646 2315 90-91 -0.5853087229405141 2315 92-93 -0.11207806051763569 2315 94-95 -0.3751662390376964 2315 96-97 -0.5700107031840709 2315 98-99 -0.8658820165308949 2315 100-101 -1.0895184035378236 2316 1 -7.356342241799073 2316 2 -6.2118072666437385 2316 3 -3.566359738542726 2316 4 -4.247001436591727 2316 5 -2.187219223803286 2316 6 -3.0249210731593514 2316 7 -3.603054552726231 2316 8 -2.813217163961383 2316 9 -6.999332786810346 2316 10-11 -7.155369775947037 2316 12-13 -6.481825805431953 2316 14-15 -5.235519659246151 2316 16-17 -5.164949967762006 2316 18-19 -4.433809099913972 2316 20-21 -3.105707990120834 2316 22-23 -3.5764178595677194 2316 24-25 -3.4505495375897937 2316 26-27 -4.355535132005002 2316 28-29 -4.566801479444663 2316 30-31 -7.315272970709316 2316 32-33 -5.093228361792107 2316 34-35 -5.769191484278622 2316 36-37 -4.645166442202552 2316 38-39 -6.721101713563115 2316 40-41 -4.12276644549668 2316 42-43 -4.941686158444853 2316 44-45 -3.4911152914152517 2316 46-47 -4.568741642557072 2316 48-49 -5.9198748637730745 2316 50-51 -5.174452302320564 2316 52-53 -6.8202772495268 2316 54-55 -5.750476802914893 2316 56-57 -5.07715906312151 2316 58-59 -3.664429326095007 2316 60-61 -3.7383081788988264 2316 62-63 -4.764181106542544 2316 64-65 -3.6959554466066145 2316 66-67 -4.611083006243156 2316 68-69 -3.6629935029542757 2316 70-71 -3.0448365787352287 2316 72-73 -2.6686733369598556 2316 74-75 -2.7433537569691744 2316 76-77 -3.39852506703253 2316 78-79 -5.234352083626497 2316 80-81 -6.147169187386194 2316 82-83 -3.7526234876885205 2316 84-85 -2.4906473961690168 2316 86-87 -2.60477423369721 2316 88-89 -1.5966028765876281 2316 90-91 -1.3371245730745045 2316 92-93 -1.258926536917489 2316 94-95 -1.7720274749508107 2316 96-97 -3.391135848490169 2316 98-99 -4.678104068626272 2316 100-101 -3.937545554258527 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 29671.0 3 9894.0 4 9593.0 5 10717.0 6 12243.0 7 14237.0 8 17086.0 9 20328.0 10 24178.0 11 28323.0 12 32595.0 13 36589.0 14 40056.0 15 45002.0 16 50267.0 17 54090.0 18 51257.0 19 50633.0 20 54398.0 21 59900.0 22 67223.0 23 74701.0 24 84228.0 25 95900.0 26 107847.0 27 122465.0 28 138618.0 29 156853.0 30 176518.0 31 199275.0 32 223237.0 33 247725.0 34 279607.0 35 319766.0 36 336968.0 37 258742.0 38 71000.0 39 1049.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.3037658914733 19.26270936748423 13.327758423274261 23.10576631776821 2 15.935148749320668 20.278916581377647 39.349874553774214 24.436060115527482 3 19.287854305993996 24.817369758682524 29.482526863094527 26.41224907222895 4 13.30025747941814 16.233759934051566 36.39094184690549 34.075040739624804 5 14.289182645405042 37.15766181244125 33.31905076538305 15.234104776770668 6 32.308860787563965 35.44112673870018 17.52044306709761 14.729569406638246 7 28.818743934562598 32.63831970054069 20.73104117565507 17.811895189241646 8 26.9626388201184 34.042000484612444 19.90418091902259 19.091179776246562 9 27.557306155290927 16.09726734351142 19.72854490207914 36.616881599118514 10-11 25.527720460262156 26.023983481465514 27.05456203911577 21.393734019156565 12-13 26.509956829366843 23.576779480469256 27.417600505655702 22.495663184508192 14-15 23.520974708253732 24.683953671027925 25.665368353143496 26.12970326757485 16-17 22.939579174983365 28.0443834553116 26.115726879574186 22.90031049013085 18-19 22.946192524652346 26.566028633179496 28.505997939919396 21.981780902248758 20-21 23.800757780252045 25.740571522529955 28.314678585297553 22.143992111920447 22-23 23.390604753971246 25.725610042772235 28.381466022071365 22.502319181185154 24-25 23.036523134027835 25.922212914261472 28.465464067040624 22.57579988467007 26-27 22.863364543238816 26.17610122575461 28.32785883622125 22.632675394785327 28-29 22.76066737640543 26.360801999254747 28.41694960494076 22.461581019399063 30-31 22.8881578536695 26.17903704489763 28.232663402670706 22.700141698762163 32-33 22.65937254600591 26.174938211888037 28.194660746956096 22.971028495149962 34-35 23.170233052052293 26.22142816126311 28.098680182655595 22.509658604029 36-37 23.161024420909886 26.01305744376388 28.036606118849207 22.789312016477027 38-39 22.87204178245627 26.106476136476488 28.12688802477033 22.89459405629691 40-41 22.88034609481683 25.95425395497799 28.392466417969963 22.77293353223521 42-43 22.90472567254906 26.060698691531027 27.998048849767258 23.03652678615266 44-45 22.791220675384082 26.07594378460779 28.04438673329614 23.088448806711988 46-47 22.837457353210226 26.21475991922337 27.788819519104695 23.15896320846171 48-49 22.854553255211297 26.40558058423354 27.48333389761177 23.25653226294339 50-51 22.65493091424975 26.57213513654523 27.52828758143815 23.244646367766865 52-53 22.949009847683392 26.419052730786692 27.334981089472826 23.29695633205709 54-55 22.574811421669903 26.309412355497052 27.521300703083124 23.59447551974992 56-57 22.570606887193094 26.543006842301885 27.40080895726582 23.4855773132392 58-59 22.629416301820623 26.228352005682165 27.45868777502178 23.683543917475433 60-61 22.516094772522177 26.23230531950937 27.63159810783362 23.62000180013483 62-63 22.4829543161667 26.188108466116578 27.622168881342457 23.70676833637426 64-65 22.359561609200597 26.184796052813137 27.51667623768047 23.938966100305798 66-67 22.421305874046404 26.393634488462002 27.242052229247953 23.943007408243638 68-69 22.537865864719713 26.155627968953958 27.419917641778905 23.88658852454742 70-71 22.41938085528755 26.19528066614859 27.27118271000603 24.11415576855783 72-73 22.538330709115865 26.30961493025048 27.064207518927592 24.087846841706064 74-75 22.56480260679706 26.486846765901472 26.930405172637457 24.017945454664016 76-77 22.729400213111887 26.34408758818202 26.94648460433803 23.98002759436806 78-79 22.6601135600464 26.422997296982274 26.873657510448524 24.043231632522797 80-81 22.71789391878764 26.53327874767548 26.82117183380055 23.92765549973632 82-83 22.749412493196523 26.455486176971732 26.733897928273514 24.061203401558227 84-85 22.678192517372437 26.46298768452881 26.647943125126773 24.210876672971978 86-87 22.778963129951318 26.337558699906843 26.812513545065077 24.07096462507676 88-89 22.752591978395046 26.271333421022984 26.61915053149363 24.356924069088343 90-91 22.60195633389789 26.311353675854864 26.850855117639146 24.235834872608102 92-93 22.948128830486684 25.973397129879377 26.966347823157268 24.112126216476675 94-95 22.87236820031619 25.953661105414195 26.782807852931406 24.39116284133821 96-97 23.389723891595327 25.794870357050755 26.282334397555797 24.533071353798125 98-99 23.301984558497658 25.86573823643426 26.083109982105984 24.7491672229621 100-101 23.675040469685822 25.82508235929971 26.055006555276723 24.444870615737745 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 5036.0 1 2988.0 2 958.0 3 1022.0 4 938.5 5 880.5 6 1226.5 7 2148.0 8 3217.0 9 3608.0 10 3335.0 11 3057.5 12 3072.5 13 3246.5 14 3461.5 15 3677.0 16 3987.0 17 4381.5 18 4725.5 19 5131.0 20 5779.5 21 6449.5 22 7431.0 23 8660.0 24 10097.0 25 12202.5 26 14791.0 27 18133.5 28 22034.0 29 26624.5 30 32334.5 31 38884.0 32 46137.0 33 51302.5 34 55453.0 35 60645.0 36 66961.5 37 74368.5 38 81341.0 39 86646.5 40 91915.5 41 99622.0 42 107955.0 43 116003.0 44 123504.0 45 129927.0 46 134664.0 47 139241.0 48 144620.5 49 147458.5 50 147048.5 51 145965.0 52 143885.5 53 141093.5 54 137320.0 55 129935.0 56 119709.5 57 108686.0 58 97729.0 59 85212.0 60 71905.5 61 60007.5 62 49207.0 63 38306.0 64 28662.5 65 22042.5 66 16878.5 67 12916.0 68 9702.0 69 7021.5 70 5155.5 71 3843.0 72 3015.0 73 2394.0 74 1845.0 75 1380.0 76 988.0 77 668.0 78 456.5 79 316.5 80 214.0 81 148.5 82 102.0 83 69.0 84 53.0 85 32.5 86 18.5 87 17.0 88 9.5 89 7.5 90 10.0 91 6.0 92 5.0 93 5.0 94 5.0 95 4.0 96 2.0 97 3.0 98 3.0 99 2.0 100 1.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.13695828059230858 2 0.12345067329056107 3 0.12923569363085868 4 0.14149772239043684 5 0.12873746221399093 6 0.14390584090529757 7 0.17379972591736167 8 0.15979388719874646 9 0.17000763124453502 10-11 0.16390429638790527 12-13 0.15449325851373694 14-15 0.1603197981387735 16-17 0.15442405970583864 18-19 0.16950939982766727 20-21 0.17505914422111069 22-23 0.20813617439649643 24-25 0.2307780243408191 26-27 0.24344140618620733 28-29 0.2390403620038757 30-31 0.22978156150708362 32-33 0.2303905110165886 34-35 0.2203843633944949 36-37 0.22821766844858213 38-39 0.21648155062903102 40-41 0.22106251171189825 42-43 0.21385199592889576 44-45 0.20669683919221188 46-47 0.22208665406879302 48-49 0.25899729820174444 50-51 0.26104558291553404 52-53 0.2628170723977304 54-55 0.2611701407697509 56-57 0.23553890232422187 58-59 0.2358018577942354 60-61 0.20741650679435417 62-63 0.2006073440971618 64-65 0.20135469122246336 66-67 0.20866208533652347 68-69 0.2090634384223336 70-71 0.21472390090841428 72-73 0.24489458115207158 74-75 0.24827148297750845 76-77 0.26316306643722187 78-79 0.2601598381744358 80-81 0.27344600929090873 82-83 0.27242186693401393 84-85 0.24739957799798992 86-87 0.25099791600870136 88-89 0.2535721116625179 90-91 0.4386374035057223 92-93 0.5912899737293645 94-95 0.34297697146711714 96-97 0.28958317129279154 98-99 0.3203904805691131 100-101 0.33892192132427695 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3612779.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 48.06682859680624 #Duplication Level Percentage of deduplicated Percentage of total 1 70.65317535801783 33.96074069753937 2 13.241638175730872 12.729671050675636 3 5.884315335559793 8.485211299317328 4 3.1445658686978164 6.045972344882602 5 1.9029259462129842 4.573380764451742 6 1.236623149565115 3.566433178139344 7 0.8476472576774841 2.8520600811735535 8 0.5971387371378946 2.2962052261216415 9 0.44153827354669084 1.910101006214868 >10 1.9193681222720176 15.319609972115597 >50 0.08768030681910348 2.914579614395281 >100 0.040503102819836205 3.5604246234337262 >500 0.0019986212745728275 0.6498111005083365 >1k 7.053957344022151E-4 0.41153572398168836 >5k 1.1756595573370233E-4 0.3846086010874517 >10k+ 5.878297786685117E-5 0.3396547159618173 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12021 0.3327355478981693 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7676 0.2124680197709298 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5936 0.1643056494737154 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 8.30385694779559E-5 2 0.0 0.0 0.0 0.0 8.30385694779559E-5 3 0.0 0.0 0.0 0.0 8.30385694779559E-5 4 0.0 2.767952315931863E-5 0.0 0.0 8.30385694779559E-5 5 0.0 1.1071809263727453E-4 0.0 0.0 8.30385694779559E-5 6 0.0 1.1071809263727453E-4 0.0 0.0 8.30385694779559E-5 7 0.0 1.1071809263727453E-4 0.0 0.0 8.30385694779559E-5 8 0.0 1.1071809263727453E-4 0.0 0.0 8.30385694779559E-5 9 0.0 1.1071809263727453E-4 0.0 0.0 1.1071809263727453E-4 10-11 0.0 1.660771389559118E-4 0.0 4.151928473897795E-5 1.1071809263727453E-4 12-13 0.0 2.4911570843386767E-4 0.0 8.30385694779559E-5 1.3839761579659314E-4 14-15 0.0 5.674302247660319E-4 0.0 8.30385694779559E-5 1.3839761579659314E-4 16-17 0.0 7.47347125301603E-4 0.0 1.3839761579659314E-4 1.3839761579659314E-4 18-19 0.0 8.027061716202402E-4 0.0 2.0759642369488972E-4 1.5223737737625248E-4 20-21 0.0 8.580652179388775E-4 0.0 4.84391655288076E-4 2.0759642369488972E-4 22-23 0.0 8.580652179388775E-4 0.0 0.0011763797342710417 2.2143618527454905E-4 24-25 0.0 9.411037874168333E-4 0.0 0.0020898039985285566 2.2143618527454905E-4 26-27 0.0 9.68783310576152E-4 0.0 0.0042211272817960915 2.2143618527454905E-4 28-29 0.0 9.68783310576152E-4 0.0 0.013189292785415328 2.2143618527454905E-4 30-31 0.0 9.68783310576152E-4 0.0 0.0444256346707064 2.2143618527454905E-4 32-33 0.0 9.68783310576152E-4 0.0 0.11736117819551099 2.4911570843386767E-4 34-35 0.0 9.68783310576152E-4 0.0 0.22046740196397288 2.4911570843386767E-4 36-37 0.0 9.68783310576152E-4 0.0 0.3824756510154649 2.4911570843386767E-4 38-39 0.0 9.68783310576152E-4 0.0 0.6345392286657999 2.4911570843386767E-4 40-41 0.0 9.68783310576152E-4 0.0 0.9191816050746531 2.4911570843386767E-4 42-43 0.0 9.68783310576152E-4 0.0 1.2060660228594111 2.6295547001352695E-4 44-45 0.0 9.68783310576152E-4 0.0 1.5418739978282647 2.767952315931863E-4 46-47 0.0 9.68783310576152E-4 0.0 1.9269100047359662 2.906349931728456E-4 48-49 0.0 9.68783310576152E-4 0.0 2.3255920165612123 3.875133242304608E-4 50-51 0.0 9.68783310576152E-4 0.0 2.7426670715258252 4.1519284738977943E-4 52-53 0.0 9.68783310576152E-4 0.0 3.154856690652819 4.5671213212875744E-4 54-55 0.0 9.68783310576152E-4 0.0 3.593590972489599 4.982314168677353E-4 56-57 0.0 9.68783310576152E-4 0.0 4.091656312218378 4.982314168677353E-4 58-59 0.0 9.68783310576152E-4 0.0 4.610287537654531 4.982314168677353E-4 60-61 0.0 9.68783310576152E-4 0.0 5.156847401958437 4.982314168677353E-4 62-63 0.0 9.68783310576152E-4 0.0 5.693318080070771 5.25910940027054E-4 64-65 0.0 9.68783310576152E-4 0.0 6.2417463121879315 5.25910940027054E-4 66-67 0.0 9.68783310576152E-4 0.0 6.830337532409262 5.25910940027054E-4 68-69 0.0 9.68783310576152E-4 0.0 7.468309021946817 5.25910940027054E-4 70-71 0.0 9.68783310576152E-4 0.0 8.139731215222408 5.25910940027054E-4 72-73 0.0 9.68783310576152E-4 0.0 8.807388993348333 5.535904631863726E-4 74-75 0.0 9.68783310576152E-4 0.0 9.464556232196877 5.535904631863726E-4 76-77 0.0 9.68783310576152E-4 0.0 10.16479280908132 5.535904631863726E-4 78-79 0.0 9.68783310576152E-4 0.0 10.911281869164984 5.535904631863726E-4 80-81 0.0 9.68783310576152E-4 0.0 11.663113077218396 5.674302247660319E-4 82-83 0.0 9.964628337354707E-4 0.0 12.383777142194416 6.089495095050098E-4 84-85 0.0 0.0010241423568947894 0.0 13.066949293051138 6.089495095050098E-4 86-87 0.0 0.0010241423568947894 0.0 13.764888469513359 6.504687942439878E-4 88-89 0.0 0.0010379821184744488 0.0 14.48995357867171 6.643085558236472E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6355 0.0 46.0564 1 GTATCAA 12525 0.0 45.259586 1 TATCAAC 16445 0.0 35.36576 2 ATCAACG 16625 0.0 34.785046 3 TCAACGC 16720 0.0 34.559025 4 CAACGCA 16955 0.0 34.025017 5 AACGCAG 17625 0.0 32.83971 6 ACGCAGA 20305 0.0 28.108084 7 GTACATG 15835 0.0 27.590652 1 TACATGG 16450 0.0 27.222782 2 CGCAGAG 20995 0.0 27.00166 8 ACATGGG 16870 0.0 25.365412 3 GCAGAGT 21200 0.0 25.286053 9 GAGTACT 10810 0.0 21.765133 12-13 CATGGGA 9260 0.0 21.671045 4 AGAGTAC 18755 0.0 21.49875 10-11 AGTACTT 11395 0.0 21.064032 12-13 CATGGGG 8285 0.0 20.842958 4 CAGAGTA 20335 0.0 19.874983 10-11 GTACTTT 11970 0.0 19.797205 14-15 >>END_MODULE