FastQCFastQC Report
Fri 27 May 2016
SRR939765_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939765_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3612779
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT131410.3637366138366061No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92240.25531592162155503No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73320.2029462638041242No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA69500.049.8652881
GTATCAA126300.041.1409261
ATCAACG169850.030.4463023
TATCAAC171750.030.330332
TCAACGC171050.030.259854
CAACGCA174100.029.7026625
AACGCAG179400.028.7805466
GTACATG161300.024.9935261
ACGCAGA208200.024.6636127
TACATGG163650.024.1113222
CGCAGAG213950.023.8906758
ACATGGG162400.023.1057763
GCAGAGT223000.022.266239
AGTACTT122050.020.34153612-13
AGAGTAC200950.020.13798910-11
GAGTACT118050.020.09298112-13
CATGGGA92450.019.732934
CATGGGG76600.019.1389944
GTACTTT125900.018.85887314-15
CAGAGTA216600.018.71558210-11
Produced by FastQC (version 0.11.5)