##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939765_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3612779 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.410503105780897 31.0 31.0 34.0 30.0 34.0 2 31.616650783233627 31.0 31.0 34.0 30.0 34.0 3 31.680561971822797 31.0 31.0 34.0 30.0 34.0 4 35.49761527068221 37.0 35.0 37.0 33.0 37.0 5 35.21961791739821 37.0 35.0 37.0 32.0 37.0 6 35.24161123611491 37.0 35.0 37.0 32.0 37.0 7 35.15262710506234 37.0 35.0 37.0 32.0 37.0 8 35.090148331796655 37.0 35.0 37.0 32.0 37.0 9 36.69834966379067 39.0 35.0 39.0 32.0 39.0 10-11 36.54704688551389 38.5 35.0 39.0 32.0 39.0 12-13 36.487267004153864 38.0 35.0 39.0 32.0 39.0 14-15 37.52382819430693 39.0 36.0 41.0 32.0 41.0 16-17 37.45214985472402 39.0 36.0 41.0 32.0 41.0 18-19 37.423330073608156 39.0 36.0 41.0 32.0 41.0 20-21 37.35007151004808 39.0 36.0 41.0 31.5 41.0 22-23 37.202169853179505 39.0 36.0 40.0 31.0 41.0 24-25 37.03293295825734 39.0 36.0 40.0 31.0 41.0 26-27 36.734986003849116 39.0 35.5 40.0 30.0 41.0 28-29 36.500718422023596 38.0 35.0 40.0 30.0 41.0 30-31 36.24072618336189 38.0 35.0 40.0 30.0 41.0 32-33 36.179330371439825 38.0 35.0 40.0 30.0 41.0 34-35 36.2474347309924 38.0 35.0 40.0 30.0 41.0 36-37 36.209630730249486 38.0 35.0 40.0 30.0 41.0 38-39 36.0680931493457 38.0 35.0 40.0 29.5 41.0 40-41 35.8888883045434 38.0 35.0 40.0 29.0 41.0 42-43 35.67179614363347 38.0 34.5 40.0 28.5 41.0 44-45 35.39615182661325 38.0 34.0 40.0 27.0 41.0 46-47 35.07549257787426 38.0 34.0 40.0 26.5 41.0 48-49 34.757793238944316 38.0 33.0 40.0 26.0 41.0 50-51 34.472085062496205 38.0 33.0 40.0 24.5 41.0 52-53 34.16902196342483 37.5 33.0 40.0 24.0 41.0 54-55 33.83734349651611 37.0 33.0 40.0 23.5 41.0 56-57 32.5561288138577 36.5 31.0 39.5 16.0 41.0 58-59 29.339770437106726 34.5 24.5 38.5 7.0 40.0 60-61 29.315941966004566 34.0 24.5 38.0 8.0 40.0 62-63 31.03896847828223 35.0 29.0 38.0 8.0 40.0 64-65 30.540085485439327 35.0 29.0 38.0 2.0 40.0 66-67 25.158408388667006 28.5 18.0 35.5 2.0 39.0 68-69 17.496558189692756 13.0 7.0 29.0 2.0 35.5 70-71 16.36398254639988 14.5 7.5 26.0 2.0 33.5 72-73 18.548994555161002 19.0 7.0 30.0 2.0 34.5 74-75 19.273212947705908 19.5 7.5 30.5 2.0 35.0 76-77 20.236324031998635 23.5 8.5 31.0 2.0 34.5 78-79 23.37299333836916 28.5 12.0 34.0 2.0 35.5 80-81 25.959655434223905 31.5 22.0 34.5 2.0 36.0 82-83 26.84630252777709 33.0 25.5 35.0 2.0 36.0 84-85 26.81641846899575 33.0 25.5 35.0 2.0 36.0 86-87 26.515685847376773 33.0 25.0 35.0 2.0 35.0 88-89 26.082338000746795 33.0 24.0 35.0 2.0 35.0 90-91 24.564621721948672 31.5 11.0 34.5 2.0 35.0 92-93 22.572747461164937 31.0 2.0 34.0 2.0 35.0 94-95 18.161339096579116 23.0 2.0 33.0 2.0 35.0 96-97 16.849422425229996 15.0 2.0 32.5 2.0 34.5 98-99 15.754293855228898 4.5 2.0 32.0 2.0 34.0 100-101 15.126153578727068 2.0 2.0 31.5 2.0 34.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.3507236626777015 1101 2 0.3403620772917968 1101 3 0.42241693158079485 1101 4 0.3090660312569753 1101 5 0.39181360504700535 1101 6 0.34000537093731964 1101 7 0.3833131186247911 1101 8 0.3457870380250796 1101 9 0.5038351220116652 1101 10-11 0.368990867086211 1101 12-13 0.45877435230232777 1101 14-15 0.4573159723281961 1101 16-17 0.46895826558354514 1101 18-19 0.4964345454860535 1101 20-21 0.6423916420336155 1101 22-23 0.5043378039110422 1101 24-25 0.4446545784048581 1101 26-27 0.46214285979225167 1101 28-29 0.14454869521193814 1101 30-31 0.34413732416572884 1101 32-33 0.39636542241029815 1101 34-35 0.5527557552318214 1101 36-37 0.5042434908568936 1101 38-39 0.5523123657628659 1101 40-41 0.5122389500668305 1101 42-43 0.4823581377238284 1101 44-45 0.36750548525213134 1101 46-47 0.2626043395790205 1101 48-49 0.3499538461090026 1101 50-51 0.23052146690402253 1101 52-53 0.4312520649915612 1101 54-55 0.2571917649503064 1101 56-57 1.0814002504401614 1101 58-59 -7.8174885904987015 1101 60-61 -9.353828554865839 1101 62-63 -2.969583574500856 1101 64-65 -0.29647695559016185 1101 66-67 -1.4044708786885032 1101 68-69 -3.9164860612424945 1101 70-71 -4.645352381166537 1101 72-73 -6.881273369501329 1101 74-75 -7.862792070163195 1101 76-77 -9.074432224683235 1101 78-79 -12.094429478013513 1101 80-81 -8.251126425758066 1101 82-83 -4.423094210955757 1101 84-85 -3.5277434653379487 1101 86-87 -3.4319919705377053 1101 88-89 -3.5128862978569266 1101 90-91 -8.614896570461902 1101 92-93 -14.722942225334387 1101 94-95 -12.74169957913827 1101 96-97 -12.467163969586279 1101 98-99 -11.852025897351979 1101 100-101 -11.584882559430095 1102 1 0.5645997407077168 1102 2 0.6093392301869223 1102 3 0.5262348254677995 1102 4 0.4962617118885291 1102 5 0.5454005026420461 1102 6 0.4880654597016232 1102 7 0.51839425876544 1102 8 0.7160357978667591 1102 9 0.8245012651468571 1102 10-11 0.765887790732009 1102 12-13 0.7075163235486315 1102 14-15 0.8997088607622175 1102 16-17 0.9341967557686743 1102 18-19 0.9193179256777881 1102 20-21 0.8605773349272994 1102 22-23 0.9006847522915251 1102 24-25 0.8654917099022228 1102 26-27 0.9836797442099225 1102 28-29 0.9604267299714451 1102 30-31 1.0232874662440636 1102 32-33 0.8580974653755078 1102 34-35 0.786761963455497 1102 36-37 0.8313538476610631 1102 38-39 0.7605791303216805 1102 40-41 0.8162595314673524 1102 42-43 0.840427943330198 1102 44-45 0.8412224128327637 1102 46-47 0.655565257431455 1102 48-49 0.644924303101206 1102 50-51 0.5560326699987144 1102 52-53 0.6093238012733266 1102 54-55 0.539685816766557 1102 56-57 1.154627628218897 1102 58-59 -9.126328177761721 1102 60-61 -10.089159012673065 1102 62-63 -3.0744568263294063 1102 64-65 -0.17225853674650082 1102 66-67 -1.3759802225895577 1102 68-69 -4.138366258492049 1102 70-71 -4.277293835606439 1102 72-73 -6.86165229627963 1102 74-75 -7.859790838606074 1102 76-77 -8.792356040499476 1102 78-79 -11.921624343619467 1102 80-81 -7.983063337432338 1102 82-83 -3.9608212114511225 1102 84-85 -3.0884438328677284 1102 86-87 -2.771561530133283 1102 88-89 -2.9704600401371515 1102 90-91 -8.137688727911488 1102 92-93 -14.53651129925487 1102 94-95 -12.377454451504926 1102 96-97 -12.231970128774808 1102 98-99 -11.72131650107551 1102 100-101 -11.467809810712843 1103 1 0.3964337001044207 1103 2 0.38027592841973856 1103 3 0.3479556659689784 1103 4 0.23407941373122299 1103 5 0.39309106490787116 1103 6 0.33576995138708554 1103 7 0.4055193682494789 1103 8 0.4835683140555105 1103 9 0.4776970222456427 1103 10-11 0.4220340030466261 1103 12-13 0.37043979891961243 1103 14-15 0.536475287690763 1103 16-17 0.5156949681197958 1103 18-19 0.5215832816020765 1103 20-21 0.5285649949024744 1103 22-23 0.5283128795362941 1103 24-25 0.5365609537676974 1103 26-27 0.4275689198260437 1103 28-29 0.5986545451491736 1103 30-31 0.5645291755375652 1103 32-33 0.5657860727193338 1103 34-35 0.6226492038429328 1103 36-37 0.47286642412577606 1103 38-39 0.5679689396063097 1103 40-41 0.6088277177776575 1103 42-43 0.6821791091385663 1103 44-45 0.6749397858691069 1103 46-47 0.5421563057619778 1103 48-49 0.5788206811879277 1103 50-51 0.5978643982866458 1103 52-53 0.5868220847125158 1103 54-55 0.5908667673725958 1103 56-57 1.1748733792055432 1103 58-59 -6.201452960144561 1103 60-61 -8.252801834494896 1103 62-63 -1.9953820928316226 1103 64-65 0.37094037608631325 1103 66-67 -1.1622148458912172 1103 68-69 -3.999331497625361 1103 70-71 -4.5150456729088955 1103 72-73 -7.529983328456829 1103 74-75 -7.841171407401916 1103 76-77 -8.721128733745557 1103 78-79 -11.881732226974506 1103 80-81 -8.079931883818148 1103 82-83 -4.1637750169325365 1103 84-85 -3.2542330461653286 1103 86-87 -3.0772962601871328 1103 88-89 -3.1157477895247965 1103 90-91 -8.6181143509348 1103 92-93 -14.204510607106814 1103 94-95 -12.387491919242109 1103 96-97 -12.40672418445742 1103 98-99 -12.068578553565887 1103 100-101 -11.733319619065643 1104 1 0.10636099418283607 1104 2 0.07390175487051209 1104 3 0.1712610341345524 1104 4 0.16178463349315564 1104 5 -0.004135460858805118 1104 6 0.15456574679302548 1104 7 0.1540391210243044 1104 8 0.16750157722880488 1104 9 0.22268335817164342 1104 10-11 0.18951472605966302 1104 12-13 0.15919779489057362 1104 14-15 0.23842539190938794 1104 16-17 0.29672299281595116 1104 18-19 0.22281703512683038 1104 20-21 0.1775000735821024 1104 22-23 0.2216273680676295 1104 24-25 0.16472878167746074 1104 26-27 0.12444941206220506 1104 28-29 0.15123908810616626 1104 30-31 0.15825006071386838 1104 32-33 0.16439752339127267 1104 34-35 0.20640391476886322 1104 36-37 0.11007389201833462 1104 38-39 0.12459603196912639 1104 40-41 0.19901241836409156 1104 42-43 0.05729840207298054 1104 44-45 0.05540813378470233 1104 46-47 0.07110242904639108 1104 48-49 -0.1242010330586254 1104 50-51 -0.004735169550357909 1104 52-53 -0.02854609404138131 1104 54-55 -0.0450323168346074 1104 56-57 0.6335915658343225 1104 58-59 -6.780695462366989 1104 60-61 -8.568562340543394 1104 62-63 -2.7607104358839045 1104 64-65 -0.464143081191299 1104 66-67 -1.7329141222587303 1104 68-69 -4.046942673500148 1104 70-71 -4.71045567091217 1104 72-73 -7.774555755297856 1104 74-75 -8.743690776646908 1104 76-77 -9.072784831375646 1104 78-79 -12.037612556441129 1104 80-81 -8.31651805974236 1104 82-83 -4.633702455987869 1104 84-85 -3.698568271008046 1104 86-87 -3.5378899208739405 1104 88-89 -3.7333093371665136 1104 90-91 -8.956930304721432 1104 92-93 -14.468490533076329 1104 94-95 -12.41688987625382 1104 96-97 -12.211420777434082 1104 98-99 -11.557731282130227 1104 100-101 -11.10287786123376 1105 1 0.1797072327516922 1105 2 0.21459259730356095 1105 3 0.11999684541625655 1105 4 0.11957947199022101 1105 5 0.15615942081649337 1105 6 0.11629386581371648 1105 7 0.20958920474340204 1105 8 0.17048456313683147 1105 9 0.27438166320417423 1105 10-11 0.2706540059773985 1105 12-13 0.28045437023565967 1105 14-15 0.25754280076714053 1105 16-17 0.27112600119533425 1105 18-19 0.25741316458311303 1105 20-21 0.32322769220205316 1105 22-23 0.32678961787309646 1105 24-25 0.27410905496535776 1105 26-27 0.34371255329984507 1105 28-29 0.272318842741754 1105 30-31 0.21244243399041096 1105 32-33 0.3615182119277449 1105 34-35 0.3224394245497777 1105 36-37 0.08641151874645203 1105 38-39 0.18273545612550635 1105 40-41 0.24730949209337894 1105 42-43 0.15547805434114537 1105 44-45 0.1786308825998688 1105 46-47 0.13562751746841428 1105 48-49 0.1678255105866313 1105 50-51 0.01179747342911952 1105 52-53 -0.03434644814958432 1105 54-55 0.12879126296321175 1105 56-57 0.7956183382075324 1105 58-59 -7.021460384648503 1105 60-61 -8.875023391674361 1105 62-63 -2.852683811950463 1105 64-65 -0.4892678196128806 1105 66-67 -1.7924178386211373 1105 68-69 -4.135516685430412 1105 70-71 -4.817904313318424 1105 72-73 -6.28574942112072 1105 74-75 -6.888421048044293 1105 76-77 -8.595102657731921 1105 78-79 -12.067734860543808 1105 80-81 -8.088551213158599 1105 82-83 -4.169527834801034 1105 84-85 -3.2797907798922594 1105 86-87 -3.035915251794272 1105 88-89 -3.2071963539612156 1105 90-91 -8.443685462653413 1105 92-93 -11.368987432879017 1105 94-95 -11.5562702008888 1105 96-97 -11.988754164292867 1105 98-99 -11.55910705741295 1105 100-101 -11.222127465511065 1106 1 0.12111874892242014 1106 2 0.14524639160588038 1106 3 0.13076084826862555 1106 4 0.11392725461578834 1106 5 0.07809988671048274 1106 6 0.0652922760565886 1106 7 0.12042836558256198 1106 8 0.23763188989659767 1106 9 0.11161504211695927 1106 10-11 0.14298525598407963 1106 12-13 0.15856739961569133 1106 14-15 0.2297950172862926 1106 16-17 0.0916534996103806 1106 18-19 0.20106659111377922 1106 20-21 0.1698785379165031 1106 22-23 0.15230608848396798 1106 24-25 0.18820209959080358 1106 26-27 0.22444889576794935 1106 28-29 0.22198998645815493 1106 30-31 0.35333906984195096 1106 32-33 0.21020469781612405 1106 34-35 0.12752138203580188 1106 36-37 0.09833336085480937 1106 38-39 0.15391689851738732 1106 40-41 0.26737235806092485 1106 42-43 0.15828091002391176 1106 44-45 0.11021018657062598 1106 46-47 0.07999817891215599 1106 48-49 -0.03265173728397741 1106 50-51 -0.04120567882300463 1106 52-53 -0.1019657173530959 1106 54-55 -0.12141908318047712 1106 56-57 0.478420362662213 1106 58-59 -7.135204260020952 1106 60-61 -9.304838206885144 1106 62-63 -2.845134871057997 1106 64-65 -0.3803707256504296 1106 66-67 -1.7394080712901605 1106 68-69 -4.1383814823060625 1106 70-71 -4.820309662899282 1106 72-73 -6.35512863539368 1106 74-75 -6.707584771112234 1106 76-77 -8.536371114470107 1106 78-79 -11.949561154185956 1106 80-81 -8.229514734807413 1106 82-83 -4.2437510909058815 1106 84-85 -3.154102945329633 1106 86-87 -2.909013583671843 1106 88-89 -3.069711695598631 1106 90-91 -8.200460105225336 1106 92-93 -11.599650956024576 1106 94-95 -11.849903795546702 1106 96-97 -12.327035064328468 1106 98-99 -11.725484576087519 1106 100-101 -11.39727865013137 1107 1 0.06510470465225282 1107 2 0.11148260714209002 1107 3 0.08553034636898715 1107 4 0.03071926646379808 1107 5 0.05961681935436047 1107 6 0.07375333875933165 1107 7 0.09183178282528814 1107 8 0.03901622662249338 1107 9 0.05346416929375408 1107 10-11 0.0955611257569231 1107 12-13 0.05718427251463254 1107 14-15 0.1899631235818049 1107 16-17 0.1734943487459759 1107 18-19 0.14232720951606836 1107 20-21 0.1026428567189086 1107 22-23 0.029434239293678388 1107 24-25 0.20616160976638298 1107 26-27 0.2601593873174082 1107 28-29 0.1885280822772728 1107 30-31 0.195728636354481 1107 32-33 0.23915443843048223 1107 34-35 0.2425288291173473 1107 36-37 0.1548948918401436 1107 38-39 0.14412393369537568 1107 40-41 0.07991548381562552 1107 42-43 0.21019879648326878 1107 44-45 0.15243108930837224 1107 46-47 0.14540890439253218 1107 48-49 0.08396819228430275 1107 50-51 0.00777229338297758 1107 52-53 -0.013938952037143792 1107 54-55 -0.003661580351014493 1107 56-57 0.6562525677136009 1107 58-59 -6.781008492235255 1107 60-61 -9.152783632820338 1107 62-63 -2.835747130553024 1107 64-65 -0.49562141250696 1107 66-67 -2.092752105420516 1107 68-69 -4.336935086567308 1107 70-71 -5.110520747690828 1107 72-73 -6.2964024018919105 1107 74-75 -6.983637723227922 1107 76-77 -9.114349053016959 1107 78-79 -12.290702440469442 1107 80-81 -8.515686987486468 1107 82-83 -4.532173771686942 1107 84-85 -3.639593232568199 1107 86-87 -3.42116032062998 1107 88-89 -3.451161362583658 1107 90-91 -8.617306813152481 1107 92-93 -11.966817320460343 1107 94-95 -11.910252504088126 1107 96-97 -12.408413100486783 1107 98-99 -11.78377218910855 1107 100-101 -11.327422553411871 1108 1 0.01791056982846584 1108 2 0.03554613681043861 1108 3 -0.036374545863068874 1108 4 0.04966552040428951 1108 5 -0.11949478021258386 1108 6 0.060436740265039646 1108 7 0.056717486297912956 1108 8 0.062349852174051534 1108 9 0.11102813123126509 1108 10-11 0.0945601001422105 1108 12-13 -0.0012756768389579065 1108 14-15 0.13846527352396976 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1.9526193076849019 2312 84-85 1.5981960696120048 2312 86-87 1.4256097663233902 2312 88-89 1.7003050836888143 2312 90-91 3.0824524831175957 2312 92-93 5.177622233683461 2312 94-95 9.17396177948805 2312 96-97 9.899689588169181 2312 98-99 10.301989239402216 2312 100-101 9.554596507274494 2313 1 -0.30834663740322554 2313 2 -0.27970618661894164 2313 3 -0.3647917772372544 2313 4 -0.3619875494211158 2313 5 -0.4842175324192084 2313 6 -0.31591054842374433 2313 7 -0.3931163910044191 2313 8 -0.35996198270346724 2313 9 -0.2864154766800766 2313 10-11 -0.4887909920388367 2313 12-13 -0.3906763145365346 2313 14-15 -0.45800048932461834 2313 16-17 -0.5231173983357067 2313 18-19 -0.4355383756487612 2313 20-21 -0.5485413038045266 2313 22-23 -0.4097383388242122 2313 24-25 -0.4499440748650372 2313 26-27 -0.4974895853843506 2313 28-29 -0.5578811585877474 2313 30-31 -0.7605115360873214 2313 32-33 -0.6134313280984074 2313 34-35 -0.612191118175275 2313 36-37 -0.698656763595686 2313 38-39 -0.917404325611713 2313 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1.1652142496007976 2314 64-65 0.9683400617268738 2314 66-67 1.5043443501149163 2314 68-69 -0.5216817761267762 2314 70-71 2.2425556688013994 2314 72-73 7.945356776789556 2314 74-75 9.522877317814487 2314 76-77 8.388164647385235 2314 78-79 6.081760339124603 2314 80-81 3.0217127584974897 2314 82-83 1.5183677763997068 2314 84-85 1.196360621184418 2314 86-87 1.1292842074411418 2314 88-89 1.3323134827723173 2314 90-91 2.5399391120068664 2314 92-93 4.701189006420666 2314 94-95 8.530086066366046 2314 96-97 9.254239485537632 2314 98-99 9.485346633950462 2314 100-101 8.628386299517546 2315 1 -0.7455854804288258 2315 2 -0.6360243584499443 2315 3 -0.6329266094918395 2315 4 -0.4200449770956922 2315 5 -0.7885126541980938 2315 6 -0.7660586644154321 2315 7 -0.733523406296591 2315 8 -0.8455615076482204 2315 9 -1.1299982382145402 2315 10-11 -0.9209938671490576 2315 12-13 -0.7626055876381344 2315 14-15 -0.9129539057442244 2315 16-17 -1.0194437781301744 2315 18-19 -0.9487046314333014 2315 20-21 -0.956003593632758 2315 22-23 -1.000306089367946 2315 24-25 -0.9620372700955144 2315 26-27 -1.1054622958416616 2315 28-29 -1.0839447698206612 2315 30-31 -1.0707336568392378 2315 32-33 -1.0142267044403042 2315 34-35 -1.126196621285679 2315 36-37 -1.239994034912435 2315 38-39 -1.3023524281493124 2315 40-41 -0.9924051237012037 2315 42-43 -1.1136476935167323 2315 44-45 -0.9526604491704163 2315 46-47 -0.9065289808312968 2315 48-49 -0.9178348849482134 2315 50-51 -0.9239606540301537 2315 52-53 -0.8350668534024734 2315 54-55 -0.9581933210942424 2315 56-57 0.25211627942093173 2315 58-59 3.7711383064864457 2315 60-61 3.3068488530657874 2315 62-63 0.7996045784860009 2315 64-65 0.7152343478260299 2315 66-67 1.737724720194052 2315 68-69 5.973214998336584 2315 70-71 4.770910170493943 2315 72-73 8.735849591558878 2315 74-75 9.750063949020625 2315 76-77 8.599028591683222 2315 78-79 5.8893480749952225 2315 80-81 3.00207409650508 2315 82-83 1.234931993167983 2315 84-85 0.705074698070348 2315 86-87 0.8349518009833581 2315 88-89 0.9125025026524831 2315 90-91 2.0738979647625264 2315 92-93 4.1506353855030405 2315 94-95 8.094354807269315 2315 96-97 8.818616001253908 2315 98-99 8.99906467169754 2315 100-101 8.270057841277277 2316 1 -0.5321203470366846 2316 2 -0.5171990106675111 2316 3 -0.6327826364764988 2316 4 -0.4622389734971293 2316 5 -0.5759631587130585 2316 6 -0.5488010989762699 2316 7 -0.6125891204560716 2316 8 -0.6936417912674457 2316 9 -0.7879949108381865 2316 10-11 -0.718057807926634 2316 12-13 -0.7014370523693145 2316 14-15 -0.7831449023269528 2316 16-17 -1.0351326471116877 2316 18-19 -1.1209296848709016 2316 20-21 -0.8404673436651251 2316 22-23 -0.9663033196013657 2316 24-25 -2.3427375350363278 2316 26-27 -1.5987816432249389 2316 28-29 -1.1556324763533752 2316 30-31 -0.8854988513246909 2316 32-33 -0.8004130649395975 2316 34-35 -0.881738820614423 2316 36-37 -0.8009332798234823 2316 38-39 -1.1376602485502119 2316 40-41 -0.8162103381143595 2316 42-43 -1.0798218429713913 2316 44-45 -1.0823058642011958 2316 46-47 -0.9913085192949467 2316 48-49 -1.0933951228007786 2316 50-51 -1.0936606569248397 2316 52-53 -1.0822243385519315 2316 54-55 -1.1381904416339381 2316 56-57 0.10566296705129474 2316 58-59 3.654260593661572 2316 60-61 3.6038082420107997 2316 62-63 1.0277639101465077 2316 64-65 0.6801498760394047 2316 66-67 5.853428367358088 2316 68-69 8.704301019547085 2316 70-71 5.389211235589551 2316 72-73 9.032092543356104 2316 74-75 9.856494096208277 2316 76-77 8.413919667336852 2316 78-79 6.060245948612813 2316 80-81 2.7936339260532144 2316 82-83 1.3335386543194936 2316 84-85 1.0285742554866353 2316 86-87 0.9666737791878219 2316 88-89 1.0438868298566781 2316 90-91 2.3681041554498314 2316 92-93 4.133435561622413 2316 94-95 8.122781098417793 2316 96-97 8.902701574548363 2316 98-99 9.167015859513006 2316 100-101 8.327855389317795 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 7.0 3 32.0 4 72.0 5 169.0 6 457.0 7 1068.0 8 2452.0 9 4380.0 10 6941.0 11 10280.0 12 14244.0 13 19181.0 14 24300.0 15 33170.0 16 44255.0 17 56520.0 18 60776.0 19 61530.0 20 64139.0 21 63411.0 22 61230.0 23 59732.0 24 62395.0 25 68604.0 26 89557.0 27 127446.0 28 175986.0 29 227292.0 30 269766.0 31 292380.0 32 304932.0 33 318242.0 34 323058.0 35 309268.0 36 255980.0 37 153203.0 38 44643.0 39 1681.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 42.43754908988019 19.37022649797328 14.36487904059721 23.82734537154931 2 15.932953217394097 21.093168265799918 38.60490917576404 24.368969341041947 3 19.052397227190102 25.090508277434893 30.01439662209462 25.842697873280386 4 13.565955736567334 16.954870475055355 36.062349786687754 33.41682400168956 5 14.024665863083632 37.5496053727798 33.479222799305134 14.946505964831424 6 32.37561234042058 35.913250136044894 17.077163335250603 14.633974188283918 7 28.496290528703806 32.72605382172561 20.728170751656826 18.049484897913768 8 26.797232931131077 34.62855156256574 19.712874531938876 18.861340974364314 9 26.99802561961305 16.10267331602625 19.662730546208333 37.23657051815237 10-11 25.359010335257153 26.257612214862853 27.098474609158213 21.28490284072178 12-13 26.240160275510902 23.60421160552583 27.52962470164934 22.626003417313928 14-15 23.133737482442516 24.917103207769205 25.58961939373205 26.359539916056228 16-17 22.69586653376805 28.226110703145697 26.160332530719426 22.91769023236683 18-19 22.747725024050368 26.650839302848635 28.542137311377086 22.059298361723908 20-21 23.493922545497526 25.887689227600134 28.33670423792875 22.281683988973587 22-23 23.119432436913524 25.903051362953562 28.40945986455302 22.568056335579893 24-25 22.884488644337225 26.13625134557082 28.40792365101768 22.571336359074277 26-27 22.775006131022867 26.385652686827356 28.23464739283443 22.60469378931536 28-29 22.68423252479454 26.464490448945245 28.34483445853367 22.50644256772654 30-31 22.762644487219074 26.386006707038817 28.116164727575168 22.735184078166938 32-33 22.619107756281647 26.306127835204496 28.100471978760886 22.97429242975297 34-35 23.060697416385338 26.25467839790121 28.085029998628467 22.599594187084985 36-37 22.981804620710765 26.140362218739632 28.03955277692682 22.838280383622788 38-39 22.73552434937023 26.141471034302132 28.16841650050582 22.954588115821817 40-41 22.790554541846497 25.971729076336697 28.36901679973011 22.868699582086695 42-43 22.784965731725837 26.097544802641625 27.990014032902916 23.127475432729625 44-45 22.65272720552436 26.143355916825602 28.041382596456977 23.162534281193064 46-47 22.757620992509654 26.23068353903272 27.74496311863654 23.26673234982108 48-49 22.69735243723098 26.407531327580053 27.521659253221426 23.373456981967546 50-51 22.537198412435835 26.54864818809017 27.565204764132154 23.348948635341838 52-53 22.89333242158074 26.334178734699655 27.382186661410245 23.390302182309362 54-55 22.48793072280568 26.230479385406024 27.563795739577678 23.717794152210615 56-57 22.48328599089301 26.382124115508628 27.48777905253299 23.646810841065378 58-59 20.790302099274378 26.119160714181128 29.338369762238052 23.752167424306446 60-61 22.385130001286942 26.225187665180133 27.7573984943471 23.63228383918582 62-63 22.278502630811932 26.302085135393604 27.5077828243923 23.911629409402167 64-65 21.50483865938125 26.22010789534775 28.035286005827874 24.23976743944313 66-67 21.387986329263487 26.200106360396646 28.41015915873082 24.00174815160905 68-69 21.794097455652665 25.551251542864055 28.90564963913877 23.74900136234451 70-71 20.958162388747446 25.851519855989064 29.059301201647862 24.131016553615627 72-73 20.839816138182634 26.009156425680015 28.893663197810803 24.257364238326545 74-75 21.486622863070316 26.082935521432354 28.19533850777023 24.235103107727095 76-77 20.161985083599447 26.413408384229236 28.77189888424428 24.652707647927038 78-79 21.190328006424426 26.497885627352836 27.57120806244078 24.740578303781966 80-81 21.859235176992282 26.59604311981622 26.840467724146883 24.704253979044612 82-83 22.00410875524147 26.534783668943497 26.60198966278811 24.859117913026918 84-85 21.903615444956763 26.449203355423446 26.48579569516865 25.161385504451143 86-87 22.047003254642114 26.3910840853592 26.516281791442708 25.045630868555975 88-89 21.84872391152682 26.331949218586193 26.44622414576482 25.37310272412217 90-91 21.501489704676573 26.391555061923416 26.7404867732618 25.366468460138208 92-93 21.401615165492004 26.180689967443538 26.95359924092684 25.464095626137617 94-95 19.854420239885652 26.181908378386794 28.150725094497986 25.812946287229572 96-97 19.710495038457545 26.02704720835002 28.28849458457288 25.97396316861955 98-99 20.07043341305424 26.014568390905584 27.707956168738807 26.20704202730137 100-101 20.07972823255698 26.041142247000497 27.61423797048738 26.264891549955138 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 633.0 1 467.5 2 327.0 3 418.0 4 517.0 5 632.5 6 876.5 7 1357.0 8 1884.0 9 2250.0 10 2459.0 11 2671.0 12 2969.5 13 3281.0 14 3567.5 15 3851.5 16 4134.5 17 4441.5 18 4850.5 19 5309.5 20 5910.0 21 6688.5 22 7599.0 23 8907.5 24 10617.0 25 12528.0 26 15216.5 27 18634.5 28 22541.0 29 27239.5 30 32839.0 31 39429.0 32 46351.0 33 52729.5 34 58344.0 35 64428.5 36 71586.0 37 78528.0 38 85302.0 39 91855.0 40 98861.5 41 107354.5 42 115734.5 43 123537.5 44 130783.5 45 137388.0 46 142737.0 47 147116.0 48 151207.0 49 153178.5 50 152515.0 51 150350.5 52 146682.0 53 141915.0 54 135711.5 55 126516.0 56 114980.0 57 102266.0 58 88996.0 59 75272.0 60 62139.5 61 50338.5 62 39528.0 63 30046.5 64 22391.0 65 16704.0 66 12410.5 67 9130.5 68 6509.5 69 4532.0 70 3315.5 71 2515.5 72 1928.5 73 1468.0 74 1081.0 75 727.0 76 455.5 77 257.0 78 142.0 79 87.5 80 48.5 81 26.0 82 17.0 83 11.5 84 6.0 85 4.0 86 1.5 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.021645387110587166 2 0.00545286606238577 3 0.0034876199180741475 4 0.0 5 0.06319235137272443 6 2.7679523159318628E-5 7 0.0 8 8.303856947795588E-5 9 0.0 10-11 0.0 12-13 0.0 14-15 9.68783310576152E-5 16-17 0.0 18-19 0.0013424568732269535 20-21 0.0 22-23 0.0 24-25 0.0 26-27 1.3839761579659314E-4 28-29 0.002463477561179358 30-31 0.008456094325171842 32-33 2.9063499317284557E-4 34-35 8.442254563592182E-4 36-37 0.023403036831203903 38-39 0.02261417042116332 40-41 0.025063808220763022 42-43 0.02520220583655961 44-45 0.025534360114471435 46-47 0.027554965305101695 48-49 0.027278170073508506 50-51 0.022987843983814125 52-53 0.01904351193361122 54-55 0.014061197764933864 56-57 0.15363519329579806 58-59 0.7557063412957172 60-61 0.31052273056281604 62-63 4.4287237054909805E-4 64-65 0.1416222802446538 66-67 1.4200425766425238 68-69 2.7296576956409457 70-71 2.206445509121925 72-73 1.8595518851277644 74-75 1.5625090823435366 76-77 1.1220309905477197 78-79 0.4223341643648837 80-81 2.3527594685420833E-4 82-83 1.6607713895591176E-4 84-85 2.7679523159318628E-5 86-87 0.0025741956538166324 88-89 1.3839761579659314E-5 90-91 0.22712432728378903 92-93 0.17370284758630405 94-95 1.2962320695508913 96-97 1.2675422437962576 98-99 0.9944007092601015 100-101 0.40288929934546236 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3612779.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 40.76575913744075 #Duplication Level Percentage of deduplicated Percentage of total 1 64.74723849090888 26.394703291348247 2 14.858907412502923 12.114692812472544 3 6.978652377090616 8.534701857252132 4 3.913077622468702 6.380783193746738 5 2.437358075962164 4.96803761281848 6 1.5830448808802176 3.8720415790632923 7 1.1038032370985649 3.1498163829081256 8 0.8223001667531683 2.681735242922963 9 0.5879578953964785 2.157169495202049 >10 2.767917908427557 18.80273062291492 >50 0.12810749494401968 3.581894932229924 >100 0.06673585204118686 4.912688854650395 >500 0.003401795513123535 0.9220618853271701 >1k 0.001292682283308792 0.7032853981043203 >5k 1.3607181929566235E-4 0.4591865382740517 >10k+ 6.803590964783118E-5 0.364470300764636 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13141 0.3637366138366061 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9224 0.25531592162155503 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7332 0.2029462638041242 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 8.30385694779559E-5 0.0 0.0 0.0 4 0.0 1.1071809263727453E-4 0.0 0.0 0.0 5 0.0 1.937566621152304E-4 0.0 0.0 0.0 6 0.0 1.937566621152304E-4 0.0 0.0 0.0 7 0.0 1.937566621152304E-4 0.0 0.0 0.0 8 0.0 1.937566621152304E-4 0.0 0.0 0.0 9 0.0 1.937566621152304E-4 0.0 0.0 0.0 10-11 0.0 1.937566621152304E-4 0.0 4.151928473897795E-5 0.0 12-13 0.0 2.62955470013527E-4 0.0 8.30385694779559E-5 2.767952315931863E-5 14-15 0.0 5.25910940027054E-4 0.0 8.30385694779559E-5 4.151928473897795E-5 16-17 0.0 7.196676021422844E-4 0.0 1.1071809263727453E-4 5.535904631863726E-5 18-19 0.0 7.473471253016031E-4 0.0 1.799169005355711E-4 8.30385694779559E-5 20-21 0.0 8.027061716202403E-4 0.0 4.5671213212875733E-4 8.30385694779559E-5 22-23 0.0 8.027061716202403E-4 0.0 0.0012040592574303604 8.30385694779559E-5 24-25 0.0 9.134242642575148E-4 0.0 0.0022143618527454903 8.30385694779559E-5 26-27 0.0 9.68783310576152E-4 0.0 0.004511762274968937 1.3839761579659314E-4 28-29 0.0 9.68783310576152E-4 0.0 0.013673684440703403 1.5223737737625248E-4 30-31 0.0 9.68783310576152E-4 0.0 0.04651543866923496 1.799169005355711E-4 32-33 0.0 9.68783310576152E-4 0.0 0.12307699972791028 2.2143618527454905E-4 34-35 0.0 9.68783310576152E-4 0.0 0.23156689075085965 2.2143618527454905E-4 36-37 0.0 9.68783310576152E-4 0.0 0.40153300271065573 2.2143618527454905E-4 38-39 0.0 9.68783310576152E-4 0.0 0.6647237486710369 2.2143618527454905E-4 40-41 0.0 9.68783310576152E-4 0.0 0.963219726421129 2.2143618527454905E-4 42-43 0.0 9.68783310576152E-4 0.0 1.2620755379722923 2.3527594685420836E-4 44-45 0.0 9.68783310576152E-4 0.0 1.6119447107060798 2.4911570843386767E-4 46-47 0.0 9.68783310576152E-4 0.0 2.0010080882334624 2.4911570843386767E-4 48-49 0.0 9.68783310576152E-4 0.0 2.373989662805281 2.4911570843386767E-4 50-51 0.0 9.964628337354707E-4 0.0 2.742141160585798 2.767952315931863E-4 52-53 0.0 0.0010241423568947894 0.0 3.1026254304511847 2.767952315931863E-4 54-55 0.0 0.0010241423568947894 0.0 3.490235633012703 2.767952315931863E-4 56-57 0.0 0.0010379821184744488 0.0 3.8292267531448783 2.767952315931863E-4 58-59 0.0 0.0010656616416337673 0.0 4.0559635671044365 2.767952315931863E-4 60-61 0.0 0.0010933411647930857 0.0 4.223770676257805 2.767952315931863E-4 62-63 0.0 0.0011210206879524046 0.0 4.371593169690147 2.767952315931863E-4 64-65 0.0 0.0011902194958507012 0.0 4.527359686269213 2.767952315931863E-4 66-67 0.0 0.0011902194958507012 0.0 4.651640745254554 2.767952315931863E-4 68-69 0.0 0.0011902194958507012 0.0 4.791629933632807 3.044747547525049E-4 70-71 0.0 0.0011902194958507012 0.0 5.043347517243651 3.044747547525049E-4 72-73 2.767952315931863E-5 0.0012178990190100196 0.0 5.36268617593271 3.4599403949148286E-4 74-75 2.767952315931863E-5 0.0012178990190100196 0.0 5.731861816070122 3.598338010711422E-4 76-77 2.767952315931863E-5 0.0012178990190100196 0.0 6.2691905594003945 3.598338010711422E-4 78-79 2.767952315931863E-5 0.0012178990190100196 0.0 7.006600735887803 3.598338010711422E-4 80-81 2.767952315931863E-5 0.0012178990190100196 0.0 7.802774540042444 3.875133242304608E-4 82-83 2.767952315931863E-5 0.0012178990190100196 0.0 8.55974029964191 4.428723705490981E-4 84-85 2.767952315931863E-5 0.0012317387805896788 0.0 9.172385025488689 4.428723705490981E-4 86-87 2.767952315931863E-5 0.0012455785421693383 0.0 9.726556758661406 4.84391655288076E-4 88-89 2.767952315931863E-5 0.001273258065328657 0.0 10.276327447651793 4.982314168677353E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6950 0.0 49.865288 1 GTATCAA 12630 0.0 41.140926 1 ATCAACG 16985 0.0 30.446302 3 TATCAAC 17175 0.0 30.33033 2 TCAACGC 17105 0.0 30.25985 4 CAACGCA 17410 0.0 29.702662 5 AACGCAG 17940 0.0 28.780546 6 GTACATG 16130 0.0 24.993526 1 ACGCAGA 20820 0.0 24.663612 7 TACATGG 16365 0.0 24.111322 2 CGCAGAG 21395 0.0 23.890675 8 ACATGGG 16240 0.0 23.105776 3 GCAGAGT 22300 0.0 22.26623 9 AGTACTT 12205 0.0 20.341536 12-13 AGAGTAC 20095 0.0 20.137989 10-11 GAGTACT 11805 0.0 20.092981 12-13 CATGGGA 9245 0.0 19.73293 4 CATGGGG 7660 0.0 19.138994 4 GTACTTT 12590 0.0 18.858873 14-15 CAGAGTA 21660 0.0 18.715582 10-11 >>END_MODULE