FastQCFastQC Report
Fri 27 May 2016
SRR939764_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939764_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3906604
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT131670.337044655665125No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83490.21371503228891386No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65310.1671784496201816No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA72750.048.6893461
GTATCAA137500.044.5430831
TCAACGC182850.033.704024
ATCAACG183300.033.5686573
TATCAAC184000.033.4907952
CAACGCA186500.032.9699945
AACGCAG192600.031.9750336
GTACATG173850.027.463971
ACGCAGA227500.026.9250957
TACATGG179750.026.2223032
CGCAGAG233600.026.0206018
ACATGGG180500.025.0718573
GCAGAGT230750.024.6095899
CATGGGG83650.021.9895424
GAGTACT119450.021.72080212-13
CATGGGA102700.021.3920214
AGAGTAC207150.020.8829410-11
AGTACTT125400.020.65267412-13
GTACTTT130800.019.78662314-15
CAGAGTA223700.019.65342110-11
Produced by FastQC (version 0.11.5)