##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939764_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3906604 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.12661610954169 31.0 30.0 33.0 26.0 34.0 2 30.183817197750272 31.0 30.0 33.0 26.0 34.0 3 30.13657642289825 31.0 30.0 34.0 26.0 34.0 4 33.803009212093166 35.0 35.0 37.0 28.0 37.0 5 33.66177554725281 35.0 33.0 37.0 28.0 37.0 6 33.644322280937615 35.0 33.0 37.0 28.0 37.0 7 33.629222209366496 35.0 33.0 37.0 28.0 37.0 8 33.46845367485417 35.0 33.0 37.0 27.0 37.0 9 34.7792962890531 37.0 34.0 39.0 27.0 39.0 10-11 34.58219683899367 37.0 34.0 39.0 27.0 39.0 12-13 34.484328588206026 37.0 33.0 39.0 27.0 39.0 14-15 35.08488753915165 38.0 33.0 40.0 26.0 41.0 16-17 34.88736406351911 38.0 33.0 40.0 25.5 41.0 18-19 34.901217784039545 38.0 33.5 40.0 25.5 41.0 20-21 35.0073704680587 38.0 34.0 40.0 25.0 41.0 22-23 34.913975539880674 38.0 33.5 40.0 25.0 41.0 24-25 34.72925039241244 38.0 33.0 40.0 25.0 41.0 26-27 34.61578573103391 38.0 33.0 40.0 24.0 41.0 28-29 34.49146202174575 38.0 33.0 40.0 24.0 41.0 30-31 34.297941383360076 38.0 33.0 40.0 23.5 41.0 32-33 34.01031752898426 38.0 33.0 40.0 23.0 41.0 34-35 33.737147020788385 38.0 32.0 40.0 21.0 41.0 36-37 33.518320515721584 37.5 32.0 40.0 19.5 41.0 38-39 33.24020696748378 37.0 31.0 40.0 18.0 41.0 40-41 32.93693512319139 37.0 31.0 40.0 16.5 41.0 42-43 32.61175883196761 37.0 31.0 40.0 15.0 41.0 44-45 32.19632972781475 37.0 30.0 39.5 13.0 41.0 46-47 31.793308075248987 36.0 30.0 39.0 9.0 40.5 48-49 31.44536213550183 36.0 30.0 39.0 8.5 40.0 50-51 30.680663435556816 35.5 28.5 38.5 7.0 40.0 52-53 30.771250042236172 35.0 29.0 38.5 6.5 40.0 54-55 30.83943061543991 35.5 29.0 39.0 2.0 40.0 56-57 30.60618020152542 35.0 28.0 39.0 2.0 40.0 58-59 30.059855439660637 35.0 28.0 38.5 2.0 40.0 60-61 29.560433691257163 34.0 27.0 38.0 2.0 40.0 62-63 29.11746801057901 34.0 26.0 38.0 2.0 40.0 64-65 28.582231907815583 34.0 26.0 37.5 2.0 39.5 66-67 28.03756625959529 33.5 25.5 37.0 2.0 39.0 68-69 27.4962331733649 33.0 24.5 36.0 2.0 39.0 70-71 26.93525745122874 32.5 22.5 36.0 2.0 39.0 72-73 26.4481480590303 32.0 22.0 35.0 2.0 38.0 74-75 25.84961542045214 31.5 20.5 35.0 2.0 37.5 76-77 25.192644685768 31.0 18.5 35.0 2.0 37.0 78-79 24.530547247686226 31.0 15.0 34.0 2.0 36.0 80-81 23.839028987837008 30.0 7.5 34.0 2.0 36.0 82-83 23.07447785851855 30.0 4.5 34.0 2.0 35.0 84-85 22.319075852069982 29.0 2.0 34.0 2.0 35.0 86-87 21.51172616932763 29.0 2.0 34.0 2.0 35.0 88-89 20.639265587195425 27.5 2.0 33.0 2.0 35.0 90-91 19.671410130128365 26.0 2.0 33.0 2.0 34.0 92-93 18.677539110695633 25.0 2.0 32.0 2.0 34.0 94-95 17.297695901606613 21.5 2.0 32.0 2.0 34.0 96-97 14.749713177992957 2.0 2.0 31.0 2.0 34.0 98-99 13.365199160191306 2.0 2.0 30.0 2.0 34.0 100-101 12.090977099291354 2.0 2.0 28.0 2.0 33.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.7406019771101597 1101 2 0.7560312975592005 1101 3 0.8447558718318859 1101 4 0.9646730582102947 1101 5 0.9392974080469898 1101 6 1.0145265177247111 1101 7 0.9315402588633788 1101 8 0.6463022053042238 1101 9 0.9044745138355879 1101 10-11 1.0083232071203696 1101 12-13 0.7663592685577143 1101 14-15 0.9871648047283514 1101 16-17 0.9772056497126016 1101 18-19 0.915699406170603 1101 20-21 0.9857220766428867 1101 22-23 0.9817266968674119 1101 24-25 1.0268916903423957 1101 26-27 1.0788172923091395 1101 28-29 1.2184366644376183 1101 30-31 1.221688322141837 1101 32-33 1.1042255248022244 1101 34-35 1.005074184606137 1101 36-37 1.1060524861133345 1101 38-39 1.0576997999536886 1101 40-41 1.154149186343794 1101 42-43 0.9896191233684561 1101 44-45 0.9825539867551285 1101 46-47 0.9038291789641875 1101 48-49 0.8134122811261797 1101 50-51 0.9471427583851231 1101 52-53 1.0329015860163686 1101 54-55 0.9427042879233909 1101 56-57 0.7025594619346371 1101 58-59 0.4407798423396123 1101 60-61 0.6514981749901487 1101 62-63 0.5468289521294487 1101 64-65 0.7477243066848835 1101 66-67 0.5354228830995744 1101 68-69 0.4806448449805778 1101 70-71 0.5168213199940404 1101 72-73 0.33216607628890316 1101 74-75 0.31006388434322574 1101 76-77 -0.055550531515272894 1101 78-79 0.09352563731968289 1101 80-81 -0.27842328439461284 1101 82-83 -0.03827728704625244 1101 84-85 -0.25638048633731003 1101 86-87 -0.40702941197103115 1101 88-89 -0.5537893208300524 1101 90-91 -0.6585155991768872 1101 92-93 -0.6506206985717569 1101 94-95 -0.2809841472675956 1101 96-97 1.0792705070362931 1101 98-99 0.9543095811725113 1101 100-101 0.4587753263683414 1102 1 0.849186875172844 1102 2 0.7559268870412588 1102 3 0.8957128979591396 1102 4 1.0422952382420547 1102 5 1.0631091687388974 1102 6 0.9711759508742261 1102 7 0.871590188177116 1102 8 0.7814415889243804 1102 9 1.0444913310710646 1102 10-11 0.8772027101725541 1102 12-13 0.8230634645137442 1102 14-15 0.9305019988701417 1102 16-17 0.9198903971520664 1102 18-19 0.9085481019301866 1102 20-21 0.934097095280336 1102 22-23 0.7535049283831441 1102 24-25 0.9804666928727386 1102 26-27 1.1881073524822483 1102 28-29 1.161283095135154 1102 30-31 1.0975992443934715 1102 32-33 1.2905944902143958 1102 34-35 1.0610919579290652 1102 36-37 1.0684994238038499 1102 38-39 1.0796929742946304 1102 40-41 1.1895240479913483 1102 42-43 1.0625594609538496 1102 44-45 0.9677090217500037 1102 46-47 1.0516130164064634 1102 48-49 0.9193497433751538 1102 50-51 0.9953114115044066 1102 52-53 0.984765684469977 1102 54-55 0.8789697950959479 1102 56-57 0.7424933221227619 1102 58-59 0.6318596266676124 1102 60-61 0.8916810355580971 1102 62-63 0.6349604419063297 1102 64-65 0.4633274348920189 1102 66-67 0.2968570931819663 1102 68-69 0.16235574089492388 1102 70-71 0.3832600656421299 1102 72-73 0.198070117649344 1102 74-75 0.04794767292002433 1102 76-77 -0.13946747543097615 1102 78-79 -0.2842643001455549 1102 80-81 -0.4608941324338467 1102 82-83 -0.5208590278981227 1102 84-85 -0.42507642413557534 1102 86-87 -0.4107716277758051 1102 88-89 -0.3646012714334681 1102 90-91 -0.4361681296797215 1102 92-93 -0.6564776184791583 1102 94-95 -0.5741761597745452 1102 96-97 0.8154822989085151 1102 98-99 0.9006787615274998 1102 100-101 0.4711738883192158 1103 1 0.7467405266180407 1103 2 0.6731938095976879 1103 3 0.8388103160316014 1103 4 0.8205709998986634 1103 5 0.8761091088101054 1103 6 0.8546278569660757 1103 7 0.8541470829049445 1103 8 0.7200649401897152 1103 9 0.947276739282465 1103 10-11 0.8708914432172605 1103 12-13 0.8063441907556168 1103 14-15 1.0496863984298983 1103 16-17 0.9195140272050537 1103 18-19 0.9266507868718818 1103 20-21 0.8231358098052084 1103 22-23 0.8962109151106361 1103 24-25 0.8644020553191467 1103 26-27 1.1623419998224591 1103 28-29 1.1438692942481552 1103 30-31 1.1017493459424088 1103 32-33 1.1316551941162842 1103 34-35 1.071056438902211 1103 36-37 0.9431805076120199 1103 38-39 0.941008651427893 1103 40-41 0.9939791197488148 1103 42-43 0.8427577948807539 1103 44-45 0.9696084277157908 1103 46-47 0.8705674557436112 1103 48-49 0.9397382693619356 1103 50-51 1.1039447473809716 1103 52-53 1.0955897211411134 1103 54-55 1.0205101735026858 1103 56-57 0.8271877803467227 1103 58-59 0.677363000763961 1103 60-61 0.910235661440101 1103 62-63 0.6838346453088633 1103 64-65 0.6867667717786645 1103 66-67 0.6035842118032164 1103 68-69 0.43104735157745466 1103 70-71 0.6329533266103518 1103 72-73 0.3631711227369543 1103 74-75 0.2957446959640926 1103 76-77 0.2288460756823376 1103 78-79 0.1596713289443521 1103 80-81 0.06356305003135887 1103 82-83 0.09261725363412054 1103 84-85 -0.13199901092605515 1103 86-87 -0.11433717944141719 1103 88-89 -0.09130837727166963 1103 90-91 -0.4123635967929431 1103 92-93 -0.3881908241175367 1103 94-95 -0.0956910235659123 1103 96-97 1.2915861238171882 1103 98-99 1.5175418775762957 1103 100-101 1.0315386777540088 1104 1 0.4978747734490163 1104 2 0.4766752037482682 1104 3 0.6695305170179218 1104 4 0.7782937889937074 1104 5 0.8014309856619093 1104 6 0.9454801775389541 1104 7 0.7607806934699752 1104 8 0.8038960396113879 1104 9 1.0418270576474242 1104 10-11 0.8705378046188983 1104 12-13 0.8254975742805399 1104 14-15 0.9164698427306561 1104 16-17 1.0561950554994937 1104 18-19 1.0222235190161584 1104 20-21 0.9377822794635478 1104 22-23 0.8764960465407299 1104 24-25 0.8912060310211842 1104 26-27 1.0173983100676338 1104 28-29 1.166738009527073 1104 30-31 1.0684487753006522 1104 32-33 1.1643715651347222 1104 34-35 1.2479464271408816 1104 36-37 1.022202850251226 1104 38-39 0.976875260598213 1104 40-41 1.061163429639329 1104 42-43 0.9011019452936608 1104 44-45 1.0515893361288065 1104 46-47 0.8954936607503896 1104 48-49 0.7497797626138016 1104 50-51 0.9896264308862825 1104 52-53 1.1566443168061546 1104 54-55 0.9720652296453878 1104 56-57 0.9009134699484314 1104 58-59 0.9246781698235758 1104 60-61 1.1213629953066402 1104 62-63 0.8994346246369815 1104 64-65 0.8550536376359652 1104 66-67 0.9880482328132665 1104 68-69 0.5879323045901756 1104 70-71 0.8388316597250203 1104 72-73 0.7893503218795637 1104 74-75 0.7916518833593003 1104 76-77 0.6282291284031025 1104 78-79 0.6517528014006224 1104 80-81 0.4285088366156238 1104 82-83 0.5253041955804285 1104 84-85 0.43972624925735815 1104 86-87 0.5816190714430043 1104 88-89 0.5990845739790451 1104 90-91 0.29080925283564696 1104 92-93 0.334417585315542 1104 94-95 0.5906476170957689 1104 96-97 1.9298696092297245 1104 98-99 2.2572103852085235 1104 100-101 2.2132078777136943 1105 1 0.7827357852053289 1105 2 0.9325566056637626 1105 3 0.8555545375298195 1105 4 0.9257590028381202 1105 5 0.8883603327803229 1105 6 1.0821036143285099 1105 7 0.9809287211486222 1105 8 0.8878653440353119 1105 9 1.1261507373285724 1105 10-11 1.0814202712800238 1105 12-13 0.8153028373512043 1105 14-15 0.9725522815800502 1105 16-17 1.0735097258999744 1105 18-19 1.0189930533603402 1105 20-21 0.9231510920235877 1105 22-23 0.7734461452960417 1105 24-25 0.98664536849396 1105 26-27 1.1339061848864418 1105 28-29 1.1543417287726498 1105 30-31 1.2251276835307934 1105 32-33 1.1041909458976065 1105 34-35 1.3520274307954097 1105 36-37 1.0920573595206164 1105 38-39 1.1121192318888333 1105 40-41 1.1165501079955575 1105 42-43 1.1291465041195181 1105 44-45 1.1125173644329749 1105 46-47 1.063154744179215 1105 48-49 0.9824168079615028 1105 50-51 1.0078726446844648 1105 52-53 1.0526261761323852 1105 54-55 0.9290253951999397 1105 56-57 0.7645654403010305 1105 58-59 0.7142803835973304 1105 60-61 0.8100095048661125 1105 62-63 0.7401707644711664 1105 64-65 0.7599440348764261 1105 66-67 0.6669611667749891 1105 68-69 0.5924584080535453 1105 70-71 0.7528089039490133 1105 72-73 0.69729543009921 1105 74-75 0.854039965136252 1105 76-77 0.8249061501207997 1105 78-79 0.6416951605953329 1105 80-81 0.34754851331174663 1105 82-83 0.14849003163501706 1105 84-85 0.20373379107058653 1105 86-87 0.1905863790794804 1105 88-89 0.23553459242674535 1105 90-91 0.3192917903219836 1105 92-93 0.2044399306442628 1105 94-95 0.4066883557962271 1105 96-97 1.7353181225794199 1105 98-99 2.0076417349084945 1105 100-101 1.5199195070910054 1106 1 0.9298687922395814 1106 2 1.0905770720936125 1106 3 0.9940929706296266 1106 4 0.9130834316007892 1106 5 0.878708060360772 1106 6 1.0002107243324119 1106 7 0.9952991641283617 1106 8 1.053309833467253 1106 9 1.2593652975878058 1106 10-11 1.1677857213860108 1106 12-13 1.0641460997669085 1106 14-15 1.3388947819810966 1106 16-17 1.383211765466875 1106 18-19 1.3192575463967913 1106 20-21 1.2480243578131223 1106 22-23 0.8808372052190023 1106 24-25 1.0851499171903782 1106 26-27 1.265670962595138 1106 28-29 1.2765927259074275 1106 30-31 1.2803709763358526 1106 32-33 1.3186835718502508 1106 34-35 1.4071436342827113 1106 36-37 1.4643060109925088 1106 38-39 1.3910785902822767 1106 40-41 1.4523900318814071 1106 42-43 1.4934114313642226 1106 44-45 1.575133981343356 1106 46-47 1.5300814514666854 1106 48-49 1.2978848452424252 1106 50-51 1.5623072050557667 1106 52-53 1.5240536892202066 1106 54-55 1.5178937001731185 1106 56-57 1.4861629813686967 1106 58-59 1.3109299039027569 1106 60-61 1.7171721428350715 1106 62-63 1.6615431349342131 1106 64-65 1.5820113340293673 1106 66-67 1.480922897545799 1106 68-69 1.4590564879721022 1106 70-71 1.6477634417446119 1106 72-73 1.726418659107452 1106 74-75 1.6387641809909255 1106 76-77 1.5730131024440261 1106 78-79 1.2787308056793414 1106 80-81 1.4154148931472825 1106 82-83 1.5586882831518487 1106 84-85 1.5222910012017117 1106 86-87 1.5131473751104885 1106 88-89 1.4259312335938823 1106 90-91 1.3074643716861871 1106 92-93 1.3790667128756553 1106 94-95 1.8115392778437283 1106 96-97 3.0825981049400344 1106 98-99 3.1448946144377263 1106 100-101 2.6805459072399813 1107 1 0.558321386392862 1107 2 0.6185924789937971 1107 3 0.6205986988361047 1107 4 0.20226061095291215 1107 5 0.6417467751355943 1107 6 0.6561130156150057 1107 7 0.5613029170912256 1107 8 0.6508950323084406 1107 9 0.7445356952701871 1107 10-11 0.8562815404536934 1107 12-13 0.7691788888108846 1107 14-15 0.8255345304667401 1107 16-17 0.8474999535100736 1107 18-19 0.7613760939343166 1107 20-21 0.7546993973179923 1107 22-23 0.7526217390615031 1107 24-25 0.8349391586968267 1107 26-27 1.0189945074990163 1107 28-29 0.9781497446063909 1107 30-31 0.9799209170785375 1107 32-33 1.139666453072273 1107 34-35 1.0556142689943258 1107 36-37 0.8577792793622763 1107 38-39 0.8629281754811231 1107 40-41 0.9567472876096161 1107 42-43 0.9686228153515799 1107 44-45 1.0014268770504913 1107 46-47 0.9149299173608085 1107 48-49 0.9456274709996002 1107 50-51 1.0141485600728863 1107 52-53 1.1745587590121467 1107 54-55 1.2165439503706494 1107 56-57 1.1266971201624862 1107 58-59 1.0363615933944637 1107 60-61 1.0678093564247497 1107 62-63 1.0130293740703777 1107 64-65 1.0571678720857243 1107 66-67 1.0186338535637063 1107 68-69 0.8679465327442948 1107 70-71 1.009893577378005 1107 72-73 0.9909524686939655 1107 74-75 1.0451941586048292 1107 76-77 0.9055480971767089 1107 78-79 0.9654554896403589 1107 80-81 0.6581637409218395 1107 82-83 0.7442517213925655 1107 84-85 0.7077471507934945 1107 86-87 0.7512226977336098 1107 88-89 0.7829997087058125 1107 90-91 0.6129242742408323 1107 92-93 0.703999554592798 1107 94-95 0.8890548822682689 1107 96-97 2.1968733551375603 1107 98-99 2.245333842775887 1107 100-101 1.83985444029239 1108 1 0.46463823164578955 1108 2 0.47194146977376406 1108 3 0.5906958206900796 1108 4 0.5433541195499743 1108 5 0.5545097953199942 1108 6 0.532497515988787 1108 7 0.6490810755761487 1108 8 0.49500193487509847 1108 9 0.6476087079788826 1108 10-11 0.5909245444407389 1108 12-13 0.3716145977568104 1108 14-15 0.37084972483504686 1108 16-17 0.5342080895678833 1108 18-19 0.6410453874802897 1108 20-21 0.5741371932312802 1108 22-23 0.42433295884720224 1108 24-25 0.5209925476826243 1108 26-27 0.6210713826547618 1108 28-29 0.7751973399216254 1108 30-31 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2.0850173558851264 1108 98-99 2.1161063944828395 1108 100-101 1.5599913702699642 1109 1 0.3647733686632364 1109 2 0.399848696674173 1109 3 -0.728557728152321 1109 4 0.0638957377689664 1109 5 0.26840178621660726 1109 6 0.2047234563931397 1109 7 0.35867148394667936 1109 8 0.24594477375143953 1109 9 0.5040294160286223 1109 10-11 0.2852203178484274 1109 12-13 0.22754542414701007 1109 14-15 0.48854558070067355 1109 16-17 0.41676756001093196 1109 18-19 0.14091287674643382 1109 20-21 0.27110567040344336 1109 22-23 0.20648313403810903 1109 24-25 0.3365220816222134 1109 26-27 0.44579738856279505 1109 28-29 0.5338611457391167 1109 30-31 0.534347874724439 1109 32-33 0.3678772385930742 1109 34-35 0.5569787112499256 1109 36-37 0.2598886120389565 1109 38-39 0.2659373173181052 1109 40-41 0.24632411597701065 1109 42-43 0.17988934453158834 1109 44-45 0.27512027153461105 1109 46-47 0.03488987180553238 1109 48-49 0.011658313156704736 1109 50-51 0.12166428895739756 1109 52-53 0.25111484125790184 1109 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2312 4 -0.20325787623896474 2312 5 -1.6918278986198416 2312 6 -0.3424838911032211 2312 7 0.1596227575938869 2312 8 0.28212412849017454 2312 9 0.3566748929502239 2312 10-11 0.29444170236766354 2312 12-13 0.3077571875059064 2312 14-15 0.5650274618345534 2312 16-17 -0.22896125963637104 2312 18-19 0.20559062994205135 2312 20-21 0.5015156732967299 2312 22-23 0.4863772368865398 2312 24-25 0.4882229914662801 2312 26-27 0.5974616413801144 2312 28-29 0.7351705275933469 2312 30-31 0.5607188266967142 2312 32-33 -0.10025331493159939 2312 34-35 0.3511623598594795 2312 36-37 0.5678150454328446 2312 38-39 0.7278489705512001 2312 40-41 0.7968263112925555 2312 42-43 0.7098796423211269 2312 44-45 0.8501508379011469 2312 46-47 0.4975912875879729 2312 48-49 0.79193547003295 2312 50-51 0.7189249617161906 2312 52-53 0.5261577198628018 2312 54-55 0.5418581118012895 2312 56-57 0.4318502738599257 2312 58-59 0.754752059803355 2312 60-61 0.8979681287785404 2312 62-63 0.9700108868784945 2312 64-65 0.8644518861021631 2312 66-67 1.0010579714950758 2312 68-69 0.8767316946582611 2312 70-71 0.8848630674698406 2312 72-73 0.991328072850564 2312 74-75 1.0964321358885414 2312 76-77 0.9711781720921273 2312 78-79 1.0887654823904818 2312 80-81 0.9929992290803717 2312 82-83 1.0791070258874917 2312 84-85 1.1576848018326409 2312 86-87 1.1899399524460321 2312 88-89 1.1428822606632814 2312 90-91 1.324798972162533 2312 92-93 1.5209207725725022 2312 94-95 1.8678684395423168 2312 96-97 3.385078707965013 2312 98-99 3.8573001308377517 2312 100-101 3.3492423088279164 2313 1 -0.16082063695344218 2313 2 -0.5593141357921922 2313 3 -0.9810929354051403 2313 4 -0.37523257970856605 2313 5 -0.368751279339115 2313 6 -0.018511498003675797 2313 7 -0.10057937862861621 2313 8 0.20201954592712212 2313 9 0.19394425558147077 2313 10-11 -0.876850268884823 2313 12-13 -0.09196216177277705 2313 14-15 0.05749513357958591 2313 16-17 -0.2870856496027727 2313 18-19 -0.06779851696062877 2313 20-21 -2.326886692176167 2313 22-23 -0.3904653231953077 2313 24-25 0.08737690188749525 2313 26-27 0.2365110444639953 2313 28-29 -0.011892084187373086 2313 30-31 0.2383114812808742 2313 32-33 0.3883989433446473 2313 34-35 0.22627866588148038 2313 36-37 -0.6578160513770186 2313 38-39 0.15016407042275404 2313 40-41 0.35755662613101435 2313 42-43 0.0749939243009976 2313 44-45 0.21764137121391514 2313 46-47 0.21117651269155147 2313 48-49 0.1269358367583564 2313 50-51 0.3583494355592691 2313 52-53 0.34902276679424915 2313 54-55 0.3553085418103379 2313 56-57 0.4316265948706537 2313 58-59 0.3775323132388202 2313 60-61 0.17285021245587373 2313 62-63 0.20100310947893263 2313 64-65 -0.0273433146847033 2313 66-67 0.3175404683497014 2313 68-69 0.055147894611994985 2313 70-71 0.390236476210692 2313 72-73 0.36293739087295407 2313 74-75 0.4766012597721456 2313 76-77 0.48056122947243196 2313 78-79 0.35625364139133353 2313 80-81 0.4221428265249543 2313 82-83 0.6431208848166889 2313 84-85 0.6008424815327942 2313 86-87 0.6380388455935311 2313 88-89 0.9649199421688515 2313 90-91 0.9545341964198215 2313 92-93 0.9861201771608918 2313 94-95 1.3101959328519293 2313 96-97 2.7643709232309437 2313 98-99 2.8058873447214143 2313 100-101 2.5683388760900563 2314 1 -0.2732071713225217 2314 2 -0.22614462203812025 2314 3 -1.1952075766618542 2314 4 -0.319328841890858 2314 5 -0.44043177058075145 2314 6 -0.6151303249177573 2314 7 -0.3758890075548038 2314 8 -1.8361558436214835 2314 9 -1.6443560733883231 2314 10-11 -0.8581424756250158 2314 12-13 -0.7477408376109977 2314 14-15 -0.8109143236249281 2314 16-17 -0.7944786282033931 2314 18-19 -1.9895635208964961 2314 20-21 -1.4585706710765294 2314 22-23 -2.119498401327263 2314 24-25 -1.9750313836597826 2314 26-27 -2.136984494180851 2314 28-29 -3.03140917068038 2314 30-31 -1.7182318007040394 2314 32-33 -1.5797700720057222 2314 34-35 -1.970009201128068 2314 36-37 -3.6455319757373132 2314 38-39 -4.000988701168751 2314 40-41 -4.588222594799795 2314 42-43 -3.3521244548668037 2314 44-45 -3.694199562834317 2314 46-47 -3.649328751945646 2314 48-49 -1.904928384531953 2314 50-51 -1.5017520578776775 2314 52-53 -2.6953585076065245 2314 54-55 -2.086677791889972 2314 56-57 -2.1578455664225906 2314 58-59 -4.166375477639857 2314 60-61 -2.6646035305442943 2314 62-63 -2.4805933161144083 2314 64-65 -1.9727968807340481 2314 66-67 -3.0290679702903844 2314 68-69 -2.7202969277788647 2314 70-71 -2.297903248875894 2314 72-73 -1.681325819043014 2314 74-75 -1.962523833955764 2314 76-77 -1.0421814744575606 2314 78-79 -0.7338557354616313 2314 80-81 -0.6924460802924592 2314 82-83 -0.6962157912064271 2314 84-85 -0.46764209806659807 2314 86-87 -0.08597240665804051 2314 88-89 0.07494139337104144 2314 90-91 0.5291364772645935 2314 92-93 0.635252254376816 2314 94-95 1.031076900506811 2314 96-97 2.5393417076693563 2314 98-99 2.7225728036590144 2314 100-101 2.3918727988237087 2315 1 -2.3326600031853033 2315 2 -1.8015700520235711 2315 3 -1.4020011770944159 2315 4 -1.3956159399155723 2315 5 -1.6782085598519991 2315 6 -2.966812542697653 2315 7 -2.1194259910973585 2315 8 -1.2085813944905652 2315 9 -1.1118116992707598 2315 10-11 -3.5854216233180125 2315 12-13 -1.804867016896317 2315 14-15 -1.2036314124612915 2315 16-17 -2.0399634459936564 2315 18-19 -1.2987997745520374 2315 20-21 -0.8085260989599163 2315 22-23 -0.9908252212362569 2315 24-25 -3.0824311886977114 2315 26-27 -4.179096063220591 2315 28-29 -5.942043803780699 2315 30-31 -8.783934852152228 2315 32-33 -7.150105259086537 2315 34-35 -6.309911785596249 2315 36-37 -3.883243053143925 2315 38-39 -6.556580819556103 2315 40-41 -4.075524424549194 2315 42-43 -3.8497329538791902 2315 44-45 -3.045945021212539 2315 46-47 -2.177267042936954 2315 48-49 -2.9446237696951876 2315 50-51 -2.856777928760863 2315 52-53 -2.1737842930970324 2315 54-55 -3.5325255285375405 2315 56-57 -3.438853438150172 2315 58-59 -2.1874867316675726 2315 60-61 -2.530529353252671 2315 62-63 -2.990337251239797 2315 64-65 -2.301034671338588 2315 66-67 -2.776307833781207 2315 68-69 -1.9111961471564527 2315 70-71 -2.376872861827948 2315 72-73 -1.4668394047551665 2315 74-75 -2.0200921317177496 2315 76-77 -1.3016221881830923 2315 78-79 -1.3243245972082711 2315 80-81 -0.9478105146612847 2315 82-83 -0.7532436260019786 2315 84-85 -0.6866027251370248 2315 86-87 -1.2698145231100604 2315 88-89 -1.7083427230559742 2315 90-91 -1.5166507877939068 2315 92-93 -0.6053800980126596 2315 94-95 0.042719274327826184 2315 96-97 1.5479357000507097 2315 98-99 1.2727972422796086 2315 100-101 1.0593197008382962 2316 1 -3.5403397040656266 2316 2 -2.2186559720594836 2316 3 -2.5097417596521225 2316 4 -3.0878301573246567 2316 5 -2.482662146797491 2316 6 -6.0254123871111425 2316 7 -3.581545595919927 2316 8 -4.718697284915844 2316 9 -6.762963685151128 2316 10-11 -5.68159314975696 2316 12-13 -3.737939450718237 2316 14-15 -4.974022315877743 2316 16-17 -4.645987964705544 2316 18-19 -2.8516169358054597 2316 20-21 -3.3965228765357836 2316 22-23 -4.186665280765013 2316 24-25 -2.864152848738115 2316 26-27 -3.93702098196815 2316 28-29 -3.769151370635239 2316 30-31 -3.5267239094394256 2316 32-33 -3.509319916196624 2316 34-35 -5.562146573632692 2316 36-37 -5.6441513278248365 2316 38-39 -5.413232450293357 2316 40-41 -4.8513437904573 2316 42-43 -4.225669470893774 2316 44-45 -3.6244657809420744 2316 46-47 -3.75836112972717 2316 48-49 -4.306251401367188 2316 50-51 -5.373680281314968 2316 52-53 -3.165745144333574 2316 54-55 -3.312359395354264 2316 56-57 -2.7750245341126067 2316 58-59 -3.4004728242015716 2316 60-61 -3.27804504258393 2316 62-63 -2.3350074908614715 2316 64-65 -2.934236248112537 2316 66-67 -3.573551400463554 2316 68-69 -2.7356104684948654 2316 70-71 -2.2644426989540882 2316 72-73 -2.2776269922725767 2316 74-75 -3.376913493191946 2316 76-77 -3.7498535447068093 2316 78-79 -3.5694762018094544 2316 80-81 -3.762965286339618 2316 82-83 -2.6779640468850054 2316 84-85 -2.526313567917782 2316 86-87 -2.1991267446455716 2316 88-89 -2.3300845539955475 2316 90-91 -1.7787036687579274 2316 92-93 -1.4746497638706444 2316 94-95 -1.6520284510650622 2316 96-97 0.22855318785131296 2316 98-99 0.6507548464933581 2316 100-101 0.3702391986567033 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 32907.0 3 10407.0 4 10089.0 5 11546.0 6 13090.0 7 15676.0 8 19398.0 9 23035.0 10 27569.0 11 32137.0 12 37506.0 13 41627.0 14 45624.0 15 50116.0 16 55928.0 17 60055.0 18 56752.0 19 56645.0 20 61323.0 21 67335.0 22 75844.0 23 84530.0 24 95112.0 25 106950.0 26 120528.0 27 136658.0 28 152848.0 29 172100.0 30 194324.0 31 218779.0 32 248080.0 33 283374.0 34 318781.0 35 347165.0 36 335232.0 37 235442.0 38 51674.0 39 418.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.36361129491989 18.54737644932012 13.574288275608264 23.51472398015173 2 15.982290162722007 20.25394145615926 39.32691957794476 24.436848803173973 3 19.298482575218966 24.850156673608865 29.436338948090366 26.4150218030818 4 13.344767489401278 16.228973973752023 36.31803940195484 34.108219134891854 5 14.366414874774758 37.13228942653478 33.27549040465272 15.225805294037736 6 32.34350248888843 35.358287152619354 17.544792302132088 14.753418056360124 7 28.779597730540523 32.67224931344826 20.69383445393855 17.854318502072665 8 27.030485577508472 33.96871335097827 19.89933414935818 19.101466922155076 9 27.559322381509055 16.088978445005026 19.729460202219077 36.62223897126684 10-11 25.554611051922976 26.020576621176684 27.05611120624507 21.36870112065527 12-13 26.526912728856022 23.572107984423802 27.407672205101758 22.493307081618422 14-15 23.597605393298977 24.706082430203868 25.605379970544163 26.09093220595299 16-17 23.012252433896183 28.012000595185228 26.07317574253651 22.902571228382083 18-19 22.983457722906188 26.50057433744174 28.520978218247716 21.99498972140436 20-21 23.793253738210083 25.73158489233937 28.33734617185207 22.137815197598478 22-23 23.40250539961235 25.745913435117888 28.358298538253983 22.493282627015773 24-25 23.03508198521357 25.907412148804305 28.44929497898611 22.608210886996016 26-27 22.895078127304984 26.163238027556535 28.31739915157377 22.62428469356471 28-29 22.858651425000126 26.309384946878783 28.39725055811238 22.43471307000871 30-31 22.877244249231605 26.185392047699434 28.179547635296352 22.75781606777261 32-33 22.61533004632893 26.25842730501344 28.165770725990956 22.960471922666667 34-35 23.215189584469602 26.198704073204077 28.07567810324351 22.51042823908281 36-37 23.15400975083841 25.98979780512531 28.054920336865795 22.80127210717049 38-39 22.89249811610343 26.0887998822212 28.13166705738177 22.887034944293603 40-41 22.990119506095837 25.90781060903511 28.385858586376678 22.716211298492368 42-43 22.876926738418028 26.040968340733983 28.01517796187224 23.06692695897575 44-45 22.805074628014072 26.060199974095955 28.027548053926576 23.107177343963397 46-47 22.901432492314985 26.21112567919673 27.74446015836658 23.142981670121706 48-49 22.895760513788794 26.375716906090783 27.47435071457766 23.254171865542762 50-51 22.65503226465382 26.586518696972977 27.50342970729572 23.255019331077484 52-53 22.94140996520447 26.427405319726034 27.339945762967666 23.29123895210183 54-55 22.6014079449901 26.258361421824766 27.54216353546182 23.59806709772331 56-57 22.59719883642988 26.52767969108304 27.366373849819915 23.508747622667165 58-59 22.633537233411392 26.21434073697788 27.48065065868256 23.67147137092817 60-61 22.579031689626035 26.204180652363167 27.600236596175165 23.61655106183563 62-63 22.509391138263794 26.170168592299376 27.62060942726271 23.69983084217412 64-65 22.390841986257133 26.186677991472802 27.475772262149263 23.94670776012081 66-67 22.410050836817508 26.367855047369737 27.233817049002752 23.98827706681 68-69 22.566566178931428 26.12958942788669 27.351496352564492 23.95234804061739 70-71 22.402985266019517 26.16635677006527 27.29496172242668 24.13569624148853 72-73 22.5697180669375 26.230241293507767 27.02946021358087 24.17058042597386 74-75 22.55808007036875 26.46971165978926 26.889052853412508 24.083155416429477 76-77 22.666470252189004 26.352090402671923 26.919429686268938 24.06200965887014 78-79 22.644600402008862 26.457987815533716 26.823159980589846 24.074251801867575 80-81 22.765459953430657 26.485323291511083 26.763425447668897 23.985791307389366 82-83 22.80921272526763 26.399984089887006 26.692409400567247 24.098393784278112 84-85 22.735241745948663 26.41033436990055 26.54634533082915 24.308078553321632 86-87 22.919488987640815 26.30485030223554 26.615019662659396 24.160641047464253 88-89 22.872367036649635 26.239799555999916 26.41284824284388 24.47498516450657 90-91 22.905204159099114 26.315651064443358 26.421355214434467 24.357789562023065 92-93 23.256313687073686 26.065937629853348 26.367521329144072 24.310227353928894 94-95 23.089179349533687 26.02797969167505 26.39348386715195 24.489357091639306 96-97 23.472437882211274 25.746236532604282 26.20146177978405 24.57986380540039 98-99 23.398743773457646 25.749999646975922 26.003201350682946 24.84805522888348 100-101 23.54421119515287 25.77515585414181 26.028864583784028 24.651768366921292 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 5149.0 1 3020.0 2 940.5 3 989.5 4 898.5 5 928.0 6 1345.5 7 2423.5 8 3651.0 9 3977.5 10 3514.5 11 3141.0 12 3194.5 13 3397.5 14 3667.0 15 3922.0 16 4211.0 17 4570.0 18 4893.5 19 5358.5 20 5994.0 21 6843.5 22 7976.5 23 9392.0 24 11109.5 25 13144.5 26 15822.5 27 19496.5 28 23963.0 29 29190.0 30 35272.5 31 42456.0 32 50482.0 33 55905.5 34 60127.5 35 65518.0 36 72397.5 37 80641.0 38 88185.0 39 94042.0 40 99822.0 41 107936.5 42 116240.0 43 124570.5 44 132966.0 45 140265.5 46 145902.0 47 150822.0 48 156574.5 49 159310.0 50 158892.0 51 157888.0 52 155392.0 53 152874.0 54 149151.5 55 140359.0 56 128840.5 57 117256.5 58 105685.0 59 92346.0 60 77759.0 61 64794.5 62 52962.5 63 41294.5 64 31177.0 65 23800.0 66 18199.0 67 14130.0 68 10609.5 69 7650.0 70 5653.0 71 4185.0 72 3270.0 73 2556.5 74 1966.0 75 1509.5 76 1059.0 77 738.5 78 494.5 79 336.0 80 243.0 81 171.5 82 124.0 83 80.0 84 47.5 85 33.0 86 21.5 87 15.0 88 10.5 89 8.5 90 9.0 91 5.5 92 4.5 93 4.5 94 3.5 95 3.5 96 2.5 97 1.0 98 0.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content warn #Base N-Count 1 0.11780052444527267 2 0.11175947190961767 3 0.12445592130658752 4 0.13891861063982938 5 0.1339782583543149 6 0.1347461887613897 7 0.1430142394775616 8 0.142809458035675 9 0.15061675050760198 10-11 0.14519004229760682 12-13 0.1446908875330082 14-15 0.132340006819222 16-17 0.13560371104928987 18-19 0.15416202922026395 20-21 0.1854423944684437 22-23 0.2033607706335221 24-25 0.22285340413310384 26-27 0.2252339883950357 28-29 0.22698742949118977 30-31 0.2268978376103644 32-33 0.2286256810262827 34-35 0.19698694825480137 36-37 0.18550638866903327 38-39 0.19889397543237042 40-41 0.18699105412271116 42-43 0.18937163838464302 44-45 0.19451677211204413 46-47 0.21586523742872327 48-49 0.2336300275123867 50-51 0.25008933590402305 52-53 0.2702475090897362 54-55 0.26332333658594526 56-57 0.23336125186991055 58-59 0.2268338434097748 60-61 0.2042182929214223 62-63 0.19409441038815298 64-65 0.18429049885783153 66-67 0.18092440390682035 68-69 0.18658149123893797 70-71 0.19080510847784932 72-73 0.22842089958439604 74-75 0.24111734898136591 76-77 0.25807581213760084 78-79 0.24765755628161956 80-81 0.2517403862792338 82-83 0.2485918716102272 84-85 0.23355323447167925 86-87 0.22355734033958907 88-89 0.2267186538487136 90-91 0.22866407754663642 92-93 0.2349483080445318 94-95 0.24022143017311198 96-97 8.720540909700599 98-99 0.2991216923957483 100-101 0.31171575107177485 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3906604.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 46.83850606376658 #Duplication Level Percentage of deduplicated Percentage of total 1 70.46399182913484 33.00428108566131 2 13.084189955945849 12.25687821182087 3 5.811994555753883 8.166754267267699 4 3.1985415695339787 5.99259634799307 5 1.9229448699490066 4.503393247569769 6 1.2952682479069406 3.640105781027613 7 0.8953463159382256 2.9355678693770555 8 0.6214612053376757 2.328665154768353 9 0.4747774021713931 2.0014077807489725 >10 2.0894406957053993 16.365315130409215 >50 0.0939393306390811 3.058744259023 >100 0.04505208524674563 3.8722925263235197 >500 0.0019976313416138745 0.6212526951157185 >1k 8.878361224805148E-4 0.5237880278265471 >5k 1.109795153100635E-4 0.38673973373983883 >10k+ 5.548975765503175E-5 0.3422178813274501 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13167 0.337044655665125 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8349 0.21371503228891386 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6531 0.1671784496201816 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 2.5597680235826308E-5 2 0.0 0.0 0.0 2.5597680235826308E-5 2.5597680235826308E-5 3 0.0 0.0 0.0 2.5597680235826308E-5 2.5597680235826308E-5 4 0.0 0.0 0.0 2.5597680235826308E-5 2.5597680235826308E-5 5 0.0 7.679304070747893E-5 0.0 2.5597680235826308E-5 2.5597680235826308E-5 6 0.0 7.679304070747893E-5 0.0 2.5597680235826308E-5 5.1195360471652616E-5 7 0.0 7.679304070747893E-5 0.0 2.5597680235826308E-5 5.1195360471652616E-5 8 0.0 1.0239072094330523E-4 0.0 2.5597680235826308E-5 5.1195360471652616E-5 9 0.0 1.2798840117913155E-4 0.0 2.5597680235826308E-5 7.679304070747893E-5 10-11 0.0 1.2798840117913155E-4 0.0 2.5597680235826308E-5 7.679304070747893E-5 12-13 0.0 1.5358608141495785E-4 0.0 6.399420058956577E-5 1.0239072094330523E-4 14-15 0.0 4.991547645986131E-4 0.0 7.679304070747893E-5 1.0239072094330523E-4 16-17 0.0 7.551315669568761E-4 0.0 1.2798840117913155E-4 1.2798840117913155E-4 18-19 0.0 7.935280873106155E-4 0.0 1.919826017686973E-4 1.407872412970447E-4 20-21 0.0 8.191257675464419E-4 0.0 3.071721628299157E-4 1.7918376165078416E-4 22-23 0.0 8.191257675464419E-4 0.0 0.0011262979303763576 1.919826017686973E-4 24-25 0.0 8.703211280180945E-4 0.0 0.0023805842619318467 2.0478144188661046E-4 26-27 0.0 8.703211280180945E-4 0.0 0.0042492149191471675 2.3037912212243677E-4 28-29 0.0 8.703211280180945E-4 0.0 0.012658052876616109 2.3037912212243677E-4 30-31 2.5597680235826308E-5 8.959188082539208E-4 0.0 0.04375923436314508 2.3037912212243677E-4 32-33 2.5597680235826308E-5 8.959188082539208E-4 0.0 0.11462641209603021 2.559768023582631E-4 34-35 2.5597680235826308E-5 8.959188082539208E-4 0.0 0.21587803626884117 2.559768023582631E-4 36-37 2.5597680235826308E-5 8.959188082539208E-4 0.0 0.38149502739463736 2.559768023582631E-4 38-39 2.5597680235826308E-5 8.959188082539208E-4 0.0 0.6354624118543881 2.559768023582631E-4 40-41 2.5597680235826308E-5 8.959188082539208E-4 0.0 0.919340685669702 2.559768023582631E-4 42-43 2.5597680235826308E-5 8.959188082539208E-4 0.0 1.2047292226189295 2.687756424761763E-4 44-45 2.5597680235826308E-5 9.215164884897471E-4 0.0 1.536820727158422 3.071721628299157E-4 46-47 2.5597680235826308E-5 9.215164884897471E-4 0.0 1.9262382365860478 3.583675233015683E-4 48-49 2.5597680235826308E-5 9.215164884897471E-4 0.0 2.3239237967298454 3.583675233015683E-4 50-51 2.5597680235826308E-5 9.215164884897471E-4 0.0 2.7412683752947573 3.583675233015683E-4 52-53 2.5597680235826308E-5 9.215164884897471E-4 0.0 3.149640966937012 3.583675233015683E-4 54-55 2.5597680235826308E-5 9.215164884897471E-4 0.0 3.5806803044280917 3.583675233015683E-4 56-57 2.5597680235826308E-5 9.215164884897471E-4 0.0 4.0683289117607 3.711663634194815E-4 58-59 2.5597680235826308E-5 9.215164884897471E-4 0.0 4.584506133716138 3.9676404365530775E-4 60-61 2.5597680235826308E-5 9.343153286076602E-4 0.0 5.126242639387048 4.095628837732209E-4 62-63 2.5597680235826308E-5 9.471141687255734E-4 0.0 5.663409959135864 4.095628837732209E-4 64-65 2.5597680235826308E-5 9.599130088434866E-4 0.0 6.20099964060857 4.095628837732209E-4 66-67 2.5597680235826308E-5 9.727118489613997E-4 0.0 6.790066257035523 4.223617238911341E-4 68-69 2.5597680235826308E-5 9.727118489613997E-4 0.0 7.4228921078256205 4.3516056400904723E-4 70-71 2.5597680235826308E-5 9.727118489613997E-4 0.0 8.094421651132286 4.6075824424487354E-4 72-73 2.5597680235826308E-5 9.727118489613997E-4 0.0 8.762892271650774 4.6075824424487354E-4 74-75 2.5597680235826308E-5 9.727118489613997E-4 0.0 9.417783322804155 4.735570843627867E-4 76-77 2.5597680235826308E-5 9.727118489613997E-4 0.0 10.11446258694252 4.8635592448069984E-4 78-79 2.5597680235826308E-5 9.727118489613997E-4 0.0 10.849295193472386 5.119536047165262E-4 80-81 2.5597680235826308E-5 9.727118489613997E-4 0.0 11.61707201446576 5.375512849523526E-4 82-83 2.5597680235826308E-5 9.727118489613997E-4 0.0 12.386013529909865 5.887466454240051E-4 84-85 2.5597680235826308E-5 9.727118489613997E-4 0.0 13.13839334624139 5.887466454240051E-4 86-87 5.1195360471652616E-5 9.727118489613997E-4 0.0 13.812405864530932 5.887466454240051E-4 88-89 5.1195360471652616E-5 9.727118489613997E-4 0.0 14.476806453891921 5.887466454240051E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 7275 0.0 48.689346 1 GTATCAA 13750 0.0 44.543083 1 TCAACGC 18285 0.0 33.70402 4 ATCAACG 18330 0.0 33.568657 3 TATCAAC 18400 0.0 33.490795 2 CAACGCA 18650 0.0 32.969994 5 AACGCAG 19260 0.0 31.975033 6 GTACATG 17385 0.0 27.46397 1 ACGCAGA 22750 0.0 26.925095 7 TACATGG 17975 0.0 26.222303 2 CGCAGAG 23360 0.0 26.020601 8 ACATGGG 18050 0.0 25.071857 3 GCAGAGT 23075 0.0 24.609589 9 CATGGGG 8365 0.0 21.989542 4 GAGTACT 11945 0.0 21.720802 12-13 CATGGGA 10270 0.0 21.392021 4 AGAGTAC 20715 0.0 20.88294 10-11 AGTACTT 12540 0.0 20.652674 12-13 GTACTTT 13080 0.0 19.786623 14-15 CAGAGTA 22370 0.0 19.653421 10-11 >>END_MODULE