FastQCFastQC Report
Fri 27 May 2016
SRR939764_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939764_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3906604
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT140940.360773705243736No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT101850.26071237320189095No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78390.20066021536864243No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA75950.049.810931
GTATCAA139250.042.1666031
ATCAACG184000.031.840243
TATCAAC185550.031.6850432
TCAACGC186050.031.463914
CAACGCA190000.030.8097935
AACGCAG195900.029.881886
GTACATG176650.025.9303131
ACGCAGA225650.025.8160767
TACATGG180100.024.9505562
CGCAGAG232050.024.8996328
ACATGGG179650.023.8708783
GCAGAGT245450.022.9604639
GAGTACT125600.021.58536512-13
AGTACTT131250.020.49351912-13
AGAGTAC219400.020.3029410-11
CATGGGG82100.019.876624
GTACTTT135800.019.71954714-15
CATGGGA105900.019.396314
CAGAGTA237400.019.25311310-11
Produced by FastQC (version 0.11.5)