##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939764_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3906604 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.38481555847483 31.0 31.0 34.0 30.0 34.0 2 31.61006106582597 31.0 31.0 34.0 30.0 34.0 3 31.653482922763608 31.0 31.0 34.0 30.0 34.0 4 35.47500668099454 37.0 35.0 37.0 33.0 37.0 5 35.19959535187083 37.0 35.0 37.0 32.0 37.0 6 35.21954823166105 37.0 35.0 37.0 32.0 37.0 7 35.11992922753368 37.0 35.0 37.0 32.0 37.0 8 35.081099850407156 37.0 35.0 37.0 32.0 37.0 9 36.66442644301803 39.0 35.0 39.0 32.0 39.0 10-11 36.52450030768412 38.0 35.0 39.0 32.0 39.0 12-13 36.43934130513357 38.0 35.0 39.0 32.0 39.0 14-15 37.46376968333622 39.0 36.0 41.0 32.0 41.0 16-17 37.38376157399112 39.0 36.0 41.0 32.0 41.0 18-19 37.361742833417466 39.0 36.0 41.0 32.0 41.0 20-21 37.27661211630357 39.0 36.0 40.5 31.0 41.0 22-23 37.14705547324479 39.0 36.0 40.0 31.0 41.0 24-25 36.98917064539943 39.0 36.0 40.0 31.0 41.0 26-27 36.68825852325959 38.5 35.5 40.0 30.0 41.0 28-29 36.4507637579852 38.0 35.0 40.0 30.0 41.0 30-31 36.17460420866819 38.0 35.0 40.0 30.0 41.0 32-33 36.14205330256151 38.0 35.0 40.0 30.0 41.0 34-35 35.980769614734434 38.0 35.0 40.0 29.5 41.0 36-37 36.0148819793355 38.0 35.0 40.0 30.0 41.0 38-39 35.90922000284647 38.0 35.0 40.0 29.0 41.0 40-41 35.81446954951154 38.0 35.0 40.0 29.0 41.0 42-43 35.578254540260545 38.0 34.0 40.0 27.5 41.0 44-45 35.31196020891802 38.0 34.0 40.0 27.0 41.0 46-47 35.003940762872304 38.0 33.5 40.0 26.0 41.0 48-49 34.690850544360266 38.0 33.0 40.0 25.5 41.0 50-51 34.44918578898706 38.0 33.0 40.0 24.0 41.0 52-53 34.185408477542126 37.5 33.0 40.0 24.0 41.0 54-55 33.90201489580208 37.0 33.0 40.0 23.5 41.0 56-57 33.51645982034524 37.0 32.0 40.0 23.0 41.0 58-59 33.12730955069928 36.0 31.5 39.0 22.0 41.0 60-61 32.79386751255055 36.0 31.0 39.0 21.5 40.0 62-63 32.47938004466283 35.0 31.0 39.0 20.5 40.0 64-65 32.03228251442941 35.0 31.0 38.5 19.5 40.0 66-67 31.125074745226286 34.5 29.5 38.0 13.0 40.0 68-69 29.243829935155958 34.0 26.0 37.0 7.5 39.0 70-71 28.597990479710766 33.0 25.0 36.0 7.5 39.0 72-73 29.147075183458576 33.0 26.5 36.0 7.5 39.0 74-75 29.236888612206407 33.0 27.5 35.5 7.0 38.0 76-77 28.757217393930894 32.5 27.0 35.0 7.0 37.0 78-79 29.28365877370729 33.5 29.0 35.0 7.0 37.0 80-81 29.101663746824606 34.0 29.0 35.0 3.5 37.0 82-83 28.668118012473236 33.5 28.0 35.0 2.0 36.0 84-85 28.319897153640348 33.0 27.0 35.0 2.0 36.0 86-87 27.93347393285831 33.0 27.0 35.0 2.0 35.0 88-89 27.479390027758125 33.0 26.0 35.0 2.0 35.0 90-91 26.981850988735996 32.5 25.0 35.0 2.0 35.0 92-93 26.485565084149812 32.0 25.0 34.0 2.0 35.0 94-95 25.670718992761998 32.0 23.0 34.0 2.0 35.0 96-97 25.122557981305505 32.0 20.0 34.0 2.0 35.0 98-99 24.432607579370725 31.0 12.5 34.0 2.0 35.0 100-101 22.975757972909463 30.0 2.0 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.24075185906542984 1101 2 0.27538716183811474 1101 3 0.34012003071237373 1101 4 0.2260103450383042 1101 5 0.1984755611160125 1101 6 0.18417227483461573 1101 7 0.1797855038265368 1101 8 0.20098456048774693 1101 9 0.31937310019872456 1101 10-11 0.31192333884823853 1101 12-13 0.35617448826473463 1101 14-15 0.3384426165081109 1101 16-17 0.4010935364475543 1101 18-19 0.26510778863647033 1101 20-21 0.33922530587388167 1101 22-23 0.3436987451310216 1101 24-25 0.30907500329544035 1101 26-27 0.21049875355532066 1101 28-29 0.34042453963363073 1101 30-31 0.1755898667656055 1101 32-33 0.06052418688349093 1101 34-35 0.01871443828085262 1101 36-37 0.20952393562207305 1101 38-39 0.2515169758821827 1101 40-41 0.23795276584471736 1101 42-43 0.06962992816063718 1101 44-45 -0.011809682211136874 1101 46-47 -0.0935977531796155 1101 48-49 -0.09895751430085653 1101 50-51 -0.12817439894880067 1101 52-53 -0.3237566153168814 1101 54-55 -0.5694235535812098 1101 56-57 -0.5331452600648134 1101 58-59 -0.3559816239961435 1101 60-61 -0.45579026791758537 1101 62-63 -0.3929773316075682 1101 64-65 -0.623241950094716 1101 66-67 -0.662849658783589 1101 68-69 -9.07204073099141 1101 70-71 -6.88896459402757 1101 72-73 -2.0922088202391755 1101 74-75 -0.9441595327264451 1101 76-77 -0.7174874688168842 1101 78-79 -0.8944680250047874 1101 80-81 -0.8409358740595074 1101 82-83 -0.7653793290764845 1101 84-85 -0.7877479545598227 1101 86-87 -0.8731181333264892 1101 88-89 -1.0039008445061768 1101 90-91 -1.3853390081417452 1101 92-93 -1.5537197899929218 1101 94-95 -1.3308428139609028 1101 96-97 -1.799393688473483 1101 98-99 -1.6941209212208825 1101 100-101 -1.566942301189183 1102 1 0.19502528973793432 1102 2 0.10721297054008616 1102 3 0.19780550188337287 1102 4 0.15462524803106703 1102 5 0.1909712677010944 1102 6 0.13540249601350496 1102 7 0.20720084900294466 1102 8 0.14986605516205742 1102 9 0.36783175608233876 1102 10-11 0.29986732502006674 1102 12-13 0.3091159196098161 1102 14-15 0.39323980706934236 1102 16-17 0.43057345507401124 1102 18-19 0.25046436653990867 1102 20-21 0.24876563159660492 1102 22-23 0.2388398401313907 1102 24-25 0.2137109935397561 1102 26-27 0.2804236677686376 1102 28-29 0.17459434745842373 1102 30-31 0.1902512801207905 1102 32-33 0.0629185547284763 1102 34-35 0.035793482280460864 1102 36-37 0.06644965872782649 1102 38-39 0.2686426475104895 1102 40-41 0.18528595796878022 1102 42-43 0.05090249035529837 1102 44-45 0.158188564236319 1102 46-47 0.15307906749495004 1102 48-49 0.08724874755500167 1102 50-51 0.11775927051157709 1102 52-53 0.16762737432668473 1102 54-55 -0.1259773915894229 1102 56-57 -0.1088932542945571 1102 58-59 -0.18178549655844023 1102 60-61 -0.034961600748118826 1102 62-63 0.05406517755334761 1102 64-65 0.019287283489909157 1102 66-67 0.32382045104947466 1102 68-69 -4.515864641394536 1102 70-71 0.08094405663211646 1102 72-73 0.37912515302520333 1102 74-75 0.11992124931717996 1102 76-77 -0.17067788693781694 1102 78-79 -0.16956698713023144 1102 80-81 -0.10393073230063621 1102 82-83 -0.1448247004051808 1102 84-85 -0.2431932657589435 1102 86-87 -0.5978454144140208 1102 88-89 -0.6813802916188152 1102 90-91 -0.5814664118283162 1102 92-93 -0.8043046589430993 1102 94-95 -0.4383173894896366 1102 96-97 -0.9702402108618173 1102 98-99 -0.7456179911964718 1102 100-101 -0.8419648089679335 1103 1 0.1809478327038363 1103 2 0.1884544283778098 1103 3 0.17285710796168274 1103 4 0.21000347392246965 1103 5 0.27280297129701125 1103 6 0.23717266568061746 1103 7 0.12142123751573308 1103 8 0.2081474612128602 1103 9 0.31276947634373187 1103 10-11 0.32461382228802194 1103 12-13 0.2734189214084708 1103 14-15 0.2669905426490047 1103 16-17 0.2663956701212058 1103 18-19 0.28295619761254187 1103 20-21 0.30545262169040654 1103 22-23 0.20533278371464547 1103 24-25 0.17440480389920054 1103 26-27 0.17809605477449253 1103 28-29 0.12359287009500264 1103 30-31 0.23187924827880124 1103 32-33 0.30590718357101565 1103 34-35 0.0821559236683953 1103 36-37 0.18106924028760574 1103 38-39 0.2762278632105648 1103 40-41 0.2553320516546336 1103 42-43 0.14215588378989708 1103 44-45 0.15642185301467038 1103 46-47 0.05985356813898335 1103 48-49 0.05920798198717847 1103 50-51 0.0071761370068657016 1103 52-53 0.1285572037883611 1103 54-55 -0.026370301353829007 1103 56-57 0.20238277611758804 1103 58-59 0.15298812970610243 1103 60-61 -0.08075679768018773 1103 62-63 -0.22738090277393042 1103 64-65 -0.1276418017459875 1103 66-67 -0.034608579141700346 1103 68-69 -4.154475901300934 1103 70-71 0.25760791870191824 1103 72-73 0.5520381857941068 1103 74-75 0.30337095758014243 1103 76-77 0.23650364105618848 1103 78-79 -0.08137514293663273 1103 80-81 -0.1410945337816436 1103 82-83 -0.2310221303842006 1103 84-85 -0.2590762425247881 1103 86-87 -0.3395175239522281 1103 88-89 -0.303817114928421 1103 90-91 -0.4199039112508096 1103 92-93 -0.5044544347117537 1103 94-95 -0.6697863259173396 1103 96-97 -0.9424310423381073 1103 98-99 -0.6983787942844657 1103 100-101 -0.8245569151585777 1104 1 0.23012344494065928 1104 2 0.2965226594914583 1104 3 0.2727614940855432 1104 4 0.2230268210293218 1104 5 0.2612309729785949 1104 6 0.2446418634236167 1104 7 0.23691375272386495 1104 8 0.31608559095440825 1104 9 0.2601540070263937 1104 10-11 0.28017353914001575 1104 12-13 0.27417011345665543 1104 14-15 0.3807710230355994 1104 16-17 0.37576449610549645 1104 18-19 0.30203940758907066 1104 20-21 0.3304959114882138 1104 22-23 0.3677146324660967 1104 24-25 0.38015799740782086 1104 26-27 0.3580692328848798 1104 28-29 0.20491908314370022 1104 30-31 0.24305034234434686 1104 32-33 0.2345569555746394 1104 34-35 0.30991258422714907 1104 36-37 0.3848120808458333 1104 38-39 0.3441249275720182 1104 40-41 0.14038845598005878 1104 42-43 0.32066160348182393 1104 44-45 0.3947300634873656 1104 46-47 0.2700149685890736 1104 48-49 0.3347826318218168 1104 50-51 0.37070656371616906 1104 52-53 0.22443434350164182 1104 54-55 0.22733707224293198 1104 56-57 0.28483118599400825 1104 58-59 0.26103187418210894 1104 60-61 0.2111537258260867 1104 62-63 0.2693211015475754 1104 64-65 0.3117406890708807 1104 66-67 0.4467916608789011 1104 68-69 -4.0307924124860435 1104 70-71 0.794381177850191 1104 72-73 1.025366437935336 1104 74-75 0.8460414045540716 1104 76-77 0.6332022494676828 1104 78-79 0.2665582299533611 1104 80-81 0.2460567023940392 1104 82-83 0.22319182019454686 1104 84-85 0.09908954850830654 1104 86-87 -0.012778946904251853 1104 88-89 0.018127332881142877 1104 90-91 0.05320193133826123 1104 92-93 -0.11939487289884454 1104 94-95 0.07189311461196368 1104 96-97 -0.1957765292059257 1104 98-99 -0.052301824276472786 1104 100-101 0.12881385738452877 1105 1 0.18886875716283313 1105 2 0.21389216834155533 1105 3 0.17033408587577625 1105 4 0.1503634455003251 1105 5 0.24219725860879038 1105 6 0.1734900030345088 1105 7 0.15489195465820416 1105 8 0.18214819151804562 1105 9 0.22896518395222643 1105 10-11 0.20170477668344944 1105 12-13 0.1808127929223602 1105 14-15 0.22844953489875763 1105 16-17 0.24593857858945256 1105 18-19 0.1480628287532184 1105 20-21 0.2014352214856956 1105 22-23 0.23135775589771868 1105 24-25 0.2544891359155912 1105 26-27 0.18770055552075604 1105 28-29 0.3349208176386327 1105 30-31 0.3195432820652897 1105 32-33 0.195593433423511 1105 34-35 0.23697087334975464 1105 36-37 0.1449599664753265 1105 38-39 0.2627855884669188 1105 40-41 0.17232624567778032 1105 42-43 0.175266133030604 1105 44-45 0.15019020243885706 1105 46-47 0.325185925368622 1105 48-49 0.2614578866474133 1105 50-51 0.25643051162101926 1105 52-53 0.2062123341316351 1105 54-55 0.32862543937669386 1105 56-57 0.3786449328880792 1105 58-59 0.43892071473828764 1105 60-61 0.4436208729096336 1105 62-63 0.46217868319063626 1105 64-65 0.32935565636655184 1105 66-67 0.869640092812002 1105 68-69 2.072962251928434 1105 70-71 2.3956468669611617 1105 72-73 1.5130039561571884 1105 74-75 0.9981238389177065 1105 76-77 0.6513025513719768 1105 78-79 0.5929736345112211 1105 80-81 0.6667037151065678 1105 82-83 0.684325458418531 1105 84-85 0.4376126185992213 1105 86-87 0.3189914303895165 1105 88-89 0.40373237692199027 1105 90-91 0.5108204760297248 1105 92-93 0.3958192144895456 1105 94-95 0.6196920629747318 1105 96-97 0.18351956988756513 1105 98-99 0.466236400216264 1105 100-101 0.5271271295976838 1106 1 0.3481697600509932 1106 2 0.35084903824731484 1106 3 0.35618916535008793 1106 4 0.22561126804102827 1106 5 0.4228617770826446 1106 6 0.3998521392401173 1106 7 0.268141470506869 1106 8 0.3059561875439698 1106 9 0.4220993114314453 1106 10-11 0.38797810087999807 1106 12-13 0.4113427159572538 1106 14-15 0.6124584604066357 1106 16-17 0.5479245275475719 1106 18-19 0.5712121040020364 1106 20-21 0.5168157850885393 1106 22-23 0.5296266762425219 1106 24-25 0.5936305151418964 1106 26-27 0.6666343852832242 1106 28-29 0.5547977498295538 1106 30-31 0.6475854406641588 1106 32-33 0.7124596617031571 1106 34-35 0.6673722866377076 1106 36-37 0.7462294001293657 1106 38-39 0.8374113542444022 1106 40-41 0.7551228348754577 1106 42-43 0.7246732862859204 1106 44-45 0.9033723221805161 1106 46-47 1.0060531482093396 1106 48-49 0.8552078744304126 1106 50-51 0.8793003076538994 1106 52-53 0.9326702189926834 1106 54-55 0.9702941921629815 1106 56-57 0.9231410499689758 1106 58-59 1.105983059981554 1106 60-61 1.081933829165493 1106 62-63 0.9749475596845798 1106 64-65 1.0090619715556173 1106 66-67 1.4380553903391338 1106 68-69 2.868381191879074 1106 70-71 3.2301051954131275 1106 72-73 2.2320775639704316 1106 74-75 1.7094128106403517 1106 76-77 1.248615503327457 1106 78-79 1.1694205143039476 1106 80-81 1.2070566422397242 1106 82-83 1.2161756182559067 1106 84-85 1.109883899202142 1106 86-87 1.2025179312325882 1106 88-89 1.3988073645062684 1106 90-91 1.216469138474114 1106 92-93 1.3085163788567904 1106 94-95 1.7347092638509132 1106 96-97 1.6115751574063992 1106 98-99 1.830602093228677 1106 100-101 1.862603280265862 1107 1 0.04190586750424785 1107 2 0.07988610698579279 1107 3 0.09277087459422972 1107 4 0.08870127330834521 1107 5 0.13936796617930014 1107 6 0.0974613043704835 1107 7 -0.03269339912704794 1107 8 0.0849381469856354 1107 9 0.07990791032531064 1107 10-11 0.05903462576806362 1107 12-13 0.06092659796937738 1107 14-15 0.1655744730481885 1107 16-17 0.17517317779776675 1107 18-19 0.20334987724406517 1107 20-21 0.13293522148569537 1107 22-23 0.22786963120697123 1107 24-25 0.15590569949797128 1107 26-27 0.15927248773252956 1107 28-29 0.14931536241844157 1107 30-31 0.1837786853310064 1107 32-33 0.18507376494256533 1107 34-35 0.14873448047494975 1107 36-37 0.06202868245751603 1107 38-39 0.13958290415222763 1107 40-41 0.036403187785644775 1107 42-43 0.13318108849819055 1107 44-45 0.16490445280995658 1107 46-47 0.11754676158831501 1107 48-49 0.1588636882307881 1107 50-51 0.32299279514401746 1107 52-53 0.23728135936666206 1107 54-55 0.3098575645622432 1107 56-57 0.23705357959762807 1107 58-59 0.16549938866208436 1107 60-61 0.2141390569769257 1107 62-63 0.3515601156498107 1107 64-65 0.21135615117110262 1107 66-67 0.775694645013882 1107 68-69 2.0457424968566897 1107 70-71 2.2552585072382563 1107 72-73 1.2764963485371972 1107 74-75 0.7440530200161746 1107 76-77 0.4632345775966158 1107 78-79 0.42448872605006116 1107 80-81 0.4424560035776217 1107 82-83 0.5488226751429259 1107 84-85 0.5312567922756166 1107 86-87 0.5564092928338518 1107 88-89 0.5797130795444545 1107 90-91 0.63564253194264 1107 92-93 0.546926834479649 1107 94-95 0.9250300763344548 1107 96-97 0.5922228727079535 1107 98-99 0.7687862517748982 1107 100-101 0.9353472557728431 1108 1 -0.17702361168837655 1108 2 -0.14340912102317915 1108 3 -0.15037558690589492 1108 4 -0.09454187396302416 1108 5 -0.030441652559794363 1108 6 0.028376330535444083 1108 7 -0.013049741015912275 1108 8 -0.036464918052992346 1108 9 -0.10243777868540604 1108 10-11 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-0.3801135044332078 2316 80-81 -0.398516889854843 2316 82-83 -0.37750895434902176 2316 84-85 -0.35251225976898937 2316 86-87 -0.42790359440283154 2316 88-89 -0.4261964619673151 2316 90-91 -0.3374121458764421 2316 92-93 -0.36066858063807317 2316 94-95 0.07344472937039015 2316 96-97 0.0801867822494664 2316 98-99 -0.30744392170713297 2316 100-101 -0.3371900738430149 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 7.0 3 36.0 4 87.0 5 203.0 6 493.0 7 1183.0 8 2595.0 9 4598.0 10 7230.0 11 10813.0 12 14862.0 13 18703.0 14 22877.0 15 28091.0 16 33831.0 17 39365.0 18 36917.0 19 31429.0 20 31639.0 21 33253.0 22 36169.0 23 41488.0 24 49132.0 25 59943.0 26 73228.0 27 89893.0 28 109521.0 29 132493.0 30 157328.0 31 186473.0 32 222539.0 33 269914.0 34 333631.0 35 421944.0 36 524774.0 37 559605.0 38 299834.0 39 20483.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 42.50068681476945 19.33030887333958 14.338080825744658 23.830923486146315 2 16.02232974186325 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23.498299290125132 25.86015884896447 28.358863606344535 22.28267825456586 22-23 23.14209477080349 25.898606564678683 28.374222214486032 22.585076450031792 24-25 22.8532896704666 26.16123050238449 28.425821484900105 22.559658342248802 26-27 22.728712602130326 26.403388428704883 28.25908634628747 22.60881262287732 28-29 22.693448857459465 26.48030858539175 28.33806165513192 22.488180902016868 30-31 22.68831812931363 26.383307682216255 28.170545264889114 22.757828923581002 32-33 22.579600584051622 26.317994298738718 28.107445941435106 22.994959175774554 34-35 23.08538448463352 26.275770372084544 28.057246218024464 22.581598925257474 36-37 22.990449687250962 26.143833808670873 28.029360741475312 22.83635576260285 38-39 22.781603579059055 26.14352440873744 28.145733840471753 22.929138171731754 40-41 22.78272931142925 25.986418491924983 28.391017565042986 22.83983463160278 42-43 22.756437852924925 26.1228893692785 27.969489451074576 23.151183326722 44-45 22.650564406733626 26.157511680428996 28.017847726255944 23.174076186581434 46-47 22.776985283520425 26.28255000240204 27.7213252838407 23.21913943023684 48-49 22.740739393215765 26.424125836588818 27.49508506872961 23.340049701465805 50-51 22.563526928412657 26.529199910375063 27.542592023457242 23.364681137755046 52-53 22.869798641901053 26.34322841043812 27.411563207343203 23.375409740317625 54-55 22.465141708322356 26.24031201841845 27.5471465654883 23.74739970777089 56-57 22.49405225107989 26.46067776810706 27.317765607863652 23.727504372949397 58-59 22.447435953889464 26.243199084916586 27.425570109459784 23.883794851734162 60-61 22.40165897539249 26.32689039521497 27.507864606779904 23.76358602261264 62-63 22.329539644055092 26.354567984412768 27.419502440976096 23.896389930556044 64-65 22.103185462431806 26.25856830439665 27.363638621002494 24.274607612169046 66-67 21.88819162388966 26.521619024972715 27.3947506221867 24.195438728950926 68-69 21.826199793269712 26.17725162324915 27.79441555769434 24.202133025786797 70-71 21.676683900008584 26.244033475753824 27.588303530907716 24.49097909332988 72-73 21.858793229967315 26.3198682603205 27.300235401461492 24.521103108250696 74-75 22.114869068926048 26.519130762081073 26.76249331069853 24.60350685829435 76-77 22.14241928578598 26.42950091463477 26.836272694311237 24.59180710526801 78-79 22.05189544114965 26.454694684701117 26.729947234195055 24.763462639954177 80-81 22.028621421538368 26.547698934827267 26.71498210889175 24.708697534742615 82-83 22.000615880501776 26.547265372822725 26.57249189960994 24.87962684706556 84-85 21.935397581648946 26.398013611623956 26.48407337606031 25.18251543066679 86-87 22.050972642972507 26.391622370295604 26.510934913996287 25.046470072735598 88-89 21.896210921221442 26.29506608822617 26.42148049094403 25.387242499608355 90-91 21.792314964064467 26.32024080403852 26.51478441879711 25.3726598130999 92-93 21.911636036536013 26.123588315070617 26.549288852277563 25.41548679611581 94-95 21.54855761046702 25.94128791444867 26.83393131090492 25.676223164179397 96-97 21.844982456230184 25.74938971234874 26.681046456107293 25.724581375313782 98-99 21.670388150103772 25.816039198914364 26.550910034231336 25.962662616750524 100-101 21.46765746304994 25.753004568613424 26.736718227195716 26.04261974114092 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1125.0 1 775.0 2 594.5 3 824.0 4 784.5 5 795.5 6 1288.0 7 2586.5 8 3955.5 9 4274.5 10 3737.5 11 3273.5 12 3371.0 13 3574.0 14 3706.5 15 4001.0 16 4360.5 17 4665.0 18 4974.0 19 5480.0 20 6140.0 21 6897.5 22 7920.5 23 9270.0 24 10839.0 25 12901.0 26 15803.0 27 19521.5 28 23709.0 29 28809.0 30 35018.5 31 42206.0 32 50415.0 33 55901.0 34 59907.0 35 65163.5 36 72080.5 37 80282.5 38 87735.5 39 93299.5 40 99047.0 41 107901.0 42 116877.0 43 125758.0 44 134454.5 45 141823.5 46 147050.0 47 151373.0 48 157839.5 49 160812.0 50 160102.0 51 159943.5 52 157275.5 53 153996.5 54 150812.0 55 142407.0 56 130211.5 57 118023.5 58 106551.5 59 92868.0 60 77734.0 61 64373.0 62 52181.5 63 39928.5 64 29487.5 65 22408.5 66 16934.0 67 13031.5 68 9466.5 69 6423.0 70 4689.5 71 3550.5 72 2729.0 73 2157.0 74 1655.0 75 1258.5 76 820.0 77 462.5 78 297.0 79 194.0 80 118.0 81 71.0 82 48.5 83 27.5 84 12.0 85 7.0 86 5.5 87 2.5 88 1.0 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.022858728450592895 2 0.024599370706629084 3 2.559768023582631E-5 4 0.0 5 0.0 6 5.119536047165262E-5 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 3.839652035373946E-4 18-19 0.0 20-21 0.0 22-23 0.0 24-25 1.1518956106121838E-4 26-27 3.7116636341948144E-4 28-29 7.679304070747893E-5 30-31 4.223617238911341E-4 32-33 0.0025597680235826307 34-35 0.0046075824424487355 36-37 0.10530885649018944 38-39 0.09702800693389962 40-41 0.10662713702233449 42-43 0.10791981987424372 44-45 0.10039410188491078 46-47 0.09385389458465716 48-49 0.09473701455279317 50-51 0.09400748066607212 52-53 0.07454044484672621 54-55 0.06336705742378802 56-57 0.034428879917186385 58-59 0.013106012280743071 60-61 0.008639217079591378 62-63 0.0018558318170974072 64-65 0.005145133727401088 66-67 0.15477637354592377 68-69 0.4102668199797062 70-71 0.39190048441050074 72-73 0.1816283401133056 74-75 0.02851581578271051 76-77 0.031229169887708096 78-79 0.004901955765160738 80-81 0.0013950735728525338 82-83 5.119536047165262E-5 84-85 4.223617238911341E-4 86-87 0.0014718666135600128 88-89 2.047814418866105E-4 90-91 6.399420058956577E-4 92-93 0.002610963384054283 94-95 0.0775481722744358 96-97 0.016868871275409536 98-99 0.017393623720243976 100-101 0.009880704571028956 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3906604.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 40.2530165804904 #Duplication Level Percentage of deduplicated Percentage of total 1 64.76880661096827 26.07139846409882 2 14.836636605142115 11.944387585309926 3 6.825361138342183 8.242241252085682 4 3.9114682986173657 6.2979359311322956 5 2.406525931640946 4.8434964113861545 6 1.609958775698196 3.8883418375251306 7 1.130106302926054 3.184313142457942 8 0.8298096600468747 2.6721873603614363 9 0.603361232857979 2.185839873923162 >10 2.87470675993208 19.443868594427567 >50 0.1284530185931367 3.592403636476017 >100 0.06965062767719553 5.07035118995305 >500 0.0036912614992180366 1.0031240873905245 >1k 0.00127284874632846 0.7373116823259301 >5k 6.364243731642294E-5 0.200819508625585 >10k+ 1.2728487463284588E-4 0.6219794425208034 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14094 0.360773705243736 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10185 0.26071237320189095 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7839 0.20066021536864243 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 2.5597680235826308E-5 2.5597680235826308E-5 2 0.0 2.5597680235826308E-5 0.0 5.1195360471652616E-5 2.5597680235826308E-5 3 0.0 7.679304070747893E-5 0.0 5.1195360471652616E-5 2.5597680235826308E-5 4 0.0 1.0239072094330523E-4 0.0 5.1195360471652616E-5 7.679304070747893E-5 5 0.0 1.0239072094330523E-4 0.0 5.1195360471652616E-5 7.679304070747893E-5 6 0.0 1.0239072094330523E-4 0.0 5.1195360471652616E-5 7.679304070747893E-5 7 0.0 1.0239072094330523E-4 0.0 5.1195360471652616E-5 7.679304070747893E-5 8 0.0 1.0239072094330523E-4 0.0 5.1195360471652616E-5 7.679304070747893E-5 9 0.0 1.0239072094330523E-4 0.0 5.1195360471652616E-5 7.679304070747893E-5 10-11 0.0 1.0239072094330523E-4 0.0 5.1195360471652616E-5 7.679304070747893E-5 12-13 0.0 2.0478144188661046E-4 0.0 8.959188082539208E-5 1.0239072094330523E-4 14-15 0.0 4.735570843627867E-4 0.0 1.0239072094330523E-4 1.2798840117913155E-4 16-17 0.0 6.399420058956577E-4 0.0 1.66384921532871E-4 1.919826017686973E-4 18-19 0.0 6.65539686131484E-4 0.0 2.687756424761763E-4 2.9437332271200253E-4 20-21 0.0 6.911373663673103E-4 0.0 3.839652035373946E-4 3.071721628299157E-4 22-23 0.0 6.911373663673103E-4 0.0 0.0012414874914375758 3.199710029478289E-4 24-25 0.0 7.807292471927025E-4 0.0 0.0025725668637005443 3.32769843065742E-4 26-27 0.0 7.935280873106156E-4 0.0 0.00458198476221291 3.583675233015683E-4 28-29 0.0 7.935280873106156E-4 0.0 0.013246799522040116 3.711663634194815E-4 30-31 0.0 7.935280873106156E-4 0.0 0.04597343370354405 3.839652035373946E-4 32-33 0.0 7.935280873106156E-4 0.0 0.12012991334673287 3.839652035373946E-4 34-35 0.0 7.935280873106156E-4 0.0 0.22639868284576578 4.095628837732209E-4 36-37 0.0 7.935280873106156E-4 0.0 0.4006548910511534 4.095628837732209E-4 38-39 0.0 7.935280873106156E-4 0.0 0.6684578216783682 4.095628837732209E-4 40-41 0.0 7.935280873106156E-4 0.0 0.9655956938558401 4.095628837732209E-4 42-43 0.0 7.935280873106156E-4 0.0 1.2643974152486406 4.223617238911341E-4 44-45 0.0 7.935280873106156E-4 0.0 1.6101580810340645 4.3516056400904723E-4 46-47 0.0 7.935280873106156E-4 0.0 2.014153469355993 4.3516056400904723E-4 48-49 0.0 7.935280873106156E-4 0.0 2.4277607866064743 4.6075824424487354E-4 50-51 0.0 7.935280873106156E-4 0.0 2.862486190051513 4.8635592448069984E-4 52-53 0.0 7.935280873106156E-4 0.0 3.287177302844107 4.8635592448069984E-4 54-55 0.0 8.063269274285287E-4 0.0 3.7355590686949585 4.991547645986131E-4 56-57 0.0 8.191257675464419E-4 0.0 4.235404970659939 5.119536047165262E-4 58-59 0.0 8.191257675464419E-4 0.0 4.741035948358216 5.119536047165262E-4 60-61 0.0 8.191257675464419E-4 0.0 5.2531175414759215 5.119536047165262E-4 62-63 0.0 8.447234477822682E-4 0.0 5.7548320740981165 5.631489651881788E-4 64-65 0.0 8.703211280180945E-4 0.0 6.269793406242353 5.631489651881788E-4 66-67 0.0 8.703211280180945E-4 0.0 6.807293495834234 5.631489651881788E-4 68-69 0.0 8.703211280180945E-4 0.0 7.392789235868289 5.631489651881788E-4 70-71 0.0 8.703211280180945E-4 0.0 8.058136939398004 5.759478053060919E-4 72-73 0.0 8.703211280180945E-4 0.0 8.739419198874522 6.271431657777445E-4 74-75 0.0 8.703211280180945E-4 0.0 9.425245046592899 6.399420058956577E-4 76-77 0.0 8.703211280180945E-4 0.0 10.160704796288542 6.399420058956577E-4 78-79 0.0 8.703211280180945E-4 0.0 10.945644861880037 6.399420058956577E-4 80-81 0.0 8.703211280180945E-4 0.0 11.765794536635912 6.911373663673103E-4 82-83 0.0 8.703211280180945E-4 0.0 12.588452784054898 7.42332726838963E-4 84-85 0.0 8.703211280180945E-4 0.0 13.393819798474583 7.42332726838963E-4 86-87 0.0 8.703211280180945E-4 0.0 14.219728951283518 7.551315669568761E-4 88-89 0.0 8.703211280180945E-4 0.0 15.076688090218513 7.679304070747892E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 7595 0.0 49.81093 1 GTATCAA 13925 0.0 42.166603 1 ATCAACG 18400 0.0 31.84024 3 TATCAAC 18555 0.0 31.685043 2 TCAACGC 18605 0.0 31.46391 4 CAACGCA 19000 0.0 30.809793 5 AACGCAG 19590 0.0 29.88188 6 GTACATG 17665 0.0 25.930313 1 ACGCAGA 22565 0.0 25.816076 7 TACATGG 18010 0.0 24.950556 2 CGCAGAG 23205 0.0 24.899632 8 ACATGGG 17965 0.0 23.870878 3 GCAGAGT 24545 0.0 22.960463 9 GAGTACT 12560 0.0 21.585365 12-13 AGTACTT 13125 0.0 20.493519 12-13 AGAGTAC 21940 0.0 20.30294 10-11 CATGGGG 8210 0.0 19.87662 4 GTACTTT 13580 0.0 19.719547 14-15 CATGGGA 10590 0.0 19.39631 4 CAGAGTA 23740 0.0 19.253113 10-11 >>END_MODULE