##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939762_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 834755 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.328824625189426 31.0 31.0 33.0 30.0 34.0 2 31.49659840312427 31.0 31.0 34.0 30.0 34.0 3 31.540816167618043 31.0 31.0 34.0 30.0 34.0 4 35.22568058891531 35.0 35.0 37.0 33.0 37.0 5 35.037030026774325 35.0 35.0 37.0 32.0 37.0 6 35.12424723421842 37.0 35.0 37.0 32.0 37.0 7 35.066885493348344 37.0 35.0 37.0 32.0 37.0 8 35.10054806500111 37.0 35.0 37.0 32.0 37.0 9 36.56802055692988 39.0 35.0 39.0 32.0 39.0 10-11 36.44363795365107 38.0 35.0 39.0 32.0 39.0 12-13 36.34780863846278 38.0 35.0 39.0 32.0 39.0 14-15 36.97455780438572 38.5 36.0 40.0 31.0 41.0 16-17 36.932448442956314 38.0 36.0 40.0 31.0 41.0 18-19 37.22208552209929 39.0 36.0 40.0 31.5 41.0 20-21 37.21450485471785 39.0 36.0 40.0 31.0 41.0 22-23 37.10720031626046 39.0 36.0 40.0 31.0 41.0 24-25 36.89702307862786 38.5 36.0 40.0 30.5 41.0 26-27 36.246998221034914 38.0 34.5 40.0 28.5 41.0 28-29 35.94918269432348 38.0 34.0 40.0 29.0 41.0 30-31 35.8340602931399 38.0 34.0 40.0 29.0 41.0 32-33 35.95235608052663 38.0 34.5 40.0 30.0 41.0 34-35 35.85703350084755 38.0 34.5 40.0 30.0 41.0 36-37 35.76951979922252 38.0 34.5 40.0 29.5 41.0 38-39 35.4298494767926 38.0 34.0 40.0 28.0 41.0 40-41 35.18246012303011 38.0 33.5 40.0 27.0 41.0 42-43 35.104990685889874 38.0 34.0 40.0 27.0 41.0 44-45 34.857754670532074 38.0 33.5 40.0 25.5 41.0 46-47 34.3478697342334 38.0 33.0 40.0 24.0 41.0 48-49 33.923577576654225 38.0 33.0 40.0 22.5 41.0 50-51 33.628338853915224 38.0 33.0 40.0 20.5 41.0 52-53 33.00759264694431 37.0 31.5 40.0 16.5 41.0 54-55 32.78244035675138 37.0 31.0 40.0 15.0 41.0 56-57 32.679199884996194 37.0 31.0 40.0 13.5 41.0 58-59 32.434812010709734 37.0 31.0 40.0 12.5 41.0 60-61 32.03289288473864 36.5 31.0 39.0 8.0 40.5 62-63 31.50121532665273 36.0 30.0 39.0 7.5 40.0 64-65 30.722911512958895 35.0 28.5 39.0 2.0 40.0 66-67 29.757973297554372 34.5 27.5 38.0 2.0 40.0 68-69 28.117821995675378 33.5 24.0 37.0 2.0 39.0 70-71 27.384690717635713 33.0 22.0 36.0 2.0 39.0 72-73 27.71174596138987 33.0 25.0 36.0 2.0 38.0 74-75 27.775841414546782 33.0 26.0 35.0 2.0 37.0 76-77 27.064297308791204 31.5 26.0 34.5 2.0 36.0 78-79 27.447927835113298 32.5 26.0 35.0 2.0 36.0 80-81 27.415870524884546 33.0 26.0 35.0 2.0 36.0 82-83 27.083737743409742 33.0 26.0 35.0 2.0 36.0 84-85 26.778265479092667 33.0 25.0 35.0 2.0 35.5 86-87 26.276402657067045 32.0 24.5 35.0 2.0 35.0 88-89 25.543850590891935 31.5 21.5 34.0 2.0 35.0 90-91 24.882508640259715 31.0 19.5 34.0 2.0 35.0 92-93 24.65240459775623 31.0 18.5 34.0 2.0 35.0 94-95 24.038937772160693 31.0 10.5 34.0 2.0 35.0 96-97 23.343083299890388 31.0 2.0 34.0 2.0 35.0 98-99 22.632304688201927 30.5 2.0 34.0 2.0 35.0 100-101 21.19041455277297 28.5 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.19407595615930617 1101 2 0.24218263775882676 1101 3 0.3367969345197537 1101 4 0.2520863842205401 1101 5 0.1205302029093005 1101 6 -0.02725185577465794 1101 7 -0.04220338068110152 1101 8 0.35768154528929585 1101 9 0.3143394947207341 1101 10-11 0.26620800336008443 1101 12-13 0.3428863990840725 1101 14-15 0.24044624363892808 1101 16-17 0.23660736579871156 1101 18-19 0.24049297567000139 1101 20-21 0.4040769793759438 1101 22-23 0.3980990170686951 1101 24-25 0.29017536013359546 1101 26-27 0.32551618694368045 1101 28-29 0.31986380805074077 1101 30-31 0.0851308512397182 1101 32-33 0.22392469046862828 1101 34-35 0.2954395102104854 1101 36-37 0.39256792692970777 1101 38-39 0.3066350784789478 1101 40-41 0.33546237343601604 1101 42-43 0.09391042297799856 1101 44-45 0.029461487159707644 1101 46-47 0.15992341423769574 1101 48-49 0.03585415212411647 1101 50-51 -0.3293298107645697 1101 52-53 -0.14983065494319447 1101 54-55 -0.222603636723953 1101 56-57 -0.3868127567698778 1101 58-59 -0.491766220440212 1101 60-61 -0.32630844004043524 1101 62-63 -0.5106240589036233 1101 64-65 -0.49727632278471745 1101 66-67 -0.5144205171781344 1101 68-69 -9.257030187256774 1101 70-71 -6.153638828332664 1101 72-73 -2.085543654576284 1101 74-75 -1.292689185879933 1101 76-77 -0.881480742118427 1101 78-79 -1.1222374658428294 1101 80-81 -0.7420591660918063 1101 82-83 -0.6538720979342223 1101 84-85 -0.7656083266878433 1101 86-87 -0.8141796540773214 1101 88-89 -0.4150952030735944 1101 90-91 -0.7095197756130815 1101 92-93 -0.8595977818701073 1101 94-95 -0.35234137685994327 1101 96-97 -0.437492474147529 1101 98-99 -0.26323487113932487 1101 100-101 -0.05859818398638694 1102 1 0.13833789395465956 1102 2 -0.048396298138811744 1102 3 0.16330221569516823 1102 4 0.1125060077607074 1102 5 0.30809698173781896 1102 6 0.1731537342445293 1102 7 0.16508474770435555 1102 8 0.12989597111097595 1102 9 0.2804530599261099 1102 10-11 0.2617288383545855 1102 12-13 0.39461440924774394 1102 14-15 0.2013025542050002 1102 16-17 0.21132576512680856 1102 18-19 0.3150666543130072 1102 20-21 0.19967263323702156 1102 22-23 0.1525409719806916 1102 24-25 0.21615185930168224 1102 26-27 -0.09859781415136126 1102 28-29 0.3260493216297675 1102 30-31 0.3809896336191443 1102 32-33 0.14046909539950292 1102 34-35 -0.0496522126699972 1102 36-37 -0.0806487793391355 1102 38-39 0.38908932335325375 1102 40-41 0.2572381188339179 1102 42-43 0.3212966531171162 1102 44-45 0.1845132889255936 1102 46-47 0.16962063039112962 1102 48-49 0.2643394089535036 1102 50-51 0.29107341375581797 1102 52-53 0.06466094886499008 1102 54-55 -0.05255683174205217 1102 56-57 -0.022304009740032882 1102 58-59 -0.3720281263411991 1102 60-61 -0.38393159216061434 1102 62-63 -0.26382279801302744 1102 64-65 -0.17025873835767413 1102 66-67 0.14608920794558955 1102 68-69 -3.9065071196566663 1102 70-71 0.15451523208589535 1102 72-73 -0.1495935214173052 1102 74-75 -0.3405553199106919 1102 76-77 -0.32909158832910634 1102 78-79 -0.47146416036390804 1102 80-81 -0.2795137830670491 1102 82-83 -0.2823200117769069 1102 84-85 -0.4001586958651302 1102 86-87 -0.6209582699681562 1102 88-89 -1.0029482338357099 1102 90-91 -0.9453221315435734 1102 92-93 -1.0496011573571593 1102 94-95 -0.7305434382991223 1102 96-97 -0.949965829062716 1102 98-99 -0.6696122824862769 1102 100-101 -0.8811698267057047 1103 1 0.27145848341086776 1103 2 0.31093329920448554 1103 3 0.3862214767993599 1103 4 0.34047404429038863 1103 5 0.45879747986982977 1103 6 0.35253833366337517 1103 7 0.33334958788699964 1103 8 0.2743698191807127 1103 9 0.28804438412701927 1103 10-11 0.43526557558995194 1103 12-13 0.35375461265164176 1103 14-15 0.4783080544125582 1103 16-17 0.4509402317104474 1103 18-19 0.3727695393275283 1103 20-21 0.40061960293399324 1103 22-23 0.4229462231222456 1103 24-25 0.4718440552335963 1103 26-27 0.5086043444206538 1103 28-29 0.6605916628501944 1103 30-31 0.5965692309624373 1103 32-33 0.38660857236089186 1103 34-35 0.21594035686092639 1103 36-37 0.16103085951517215 1103 38-39 0.600206488151926 1103 40-41 0.6585898207849397 1103 42-43 0.7601535439431828 1103 44-45 0.37590753965202595 1103 46-47 0.23845884126701122 1103 48-49 -0.025160902378999594 1103 50-51 0.08179570516303869 1103 52-53 0.20466717551505553 1103 54-55 -0.037130306212780795 1103 56-57 0.01683048590130909 1103 58-59 0.23334754821255643 1103 60-61 -0.035869636992494236 1103 62-63 0.09874465736264781 1103 64-65 0.1466284554986963 1103 66-67 0.39361670898336243 1103 68-69 -3.3814137199057335 1103 70-71 0.6679388518451326 1103 72-73 0.9882022444606484 1103 74-75 0.7420671375405341 1103 76-77 0.734044048864746 1103 78-79 0.29799152664315187 1103 80-81 0.5011550629271362 1103 82-83 0.43954850212928065 1103 84-85 0.5850140936741006 1103 86-87 0.42609444900237037 1103 88-89 0.43442723316304566 1103 90-91 0.2141553487387 1103 92-93 0.3585972652248266 1103 94-95 0.3464283342205974 1103 96-97 -0.04835208892157894 1103 98-99 0.28461146180595875 1103 100-101 0.2110857772793544 1104 1 0.08623881177815207 1104 2 0.07267699029599939 1104 3 0.0174125683165407 1104 4 -0.04166084980103335 1104 5 0.21304269118025587 1104 6 -0.05314855191480916 1104 7 -0.03766490486662377 1104 8 -0.07136469992337169 1104 9 0.06258451790628783 1104 10-11 0.09379653738177751 1104 12-13 -0.1461015999763049 1104 14-15 -0.010915493778384189 1104 16-17 0.03813048710722455 1104 18-19 -0.056352154196339654 1104 20-21 0.1414229119438204 1104 22-23 0.006523424905971353 1104 24-25 0.05831790746832155 1104 26-27 0.0786864362418811 1104 28-29 -0.19656983507216808 1104 30-31 0.030769722499890406 1104 32-33 -0.20800985269232086 1104 34-35 0.11064619450154822 1104 36-37 -0.020241943768503745 1104 38-39 0.28441964818030385 1104 40-41 0.0443003964411659 1104 42-43 0.08107238959028962 1104 44-45 0.19690619679199273 1104 46-47 0.2609333776002458 1104 48-49 0.514070246906492 1104 50-51 0.5338403995990362 1104 52-53 0.3193474228040003 1104 54-55 0.31382616485237946 1104 56-57 0.42647120749855105 1104 58-59 0.35159017515083946 1104 60-61 0.4536338848479886 1104 62-63 0.39399397326413776 1104 64-65 0.2860052924298806 1104 66-67 0.08109707282242695 1104 68-69 -3.5110985278514093 1104 70-71 0.7058163728748248 1104 72-73 1.0491148825472045 1104 74-75 0.7687989202505783 1104 76-77 0.4825713367942086 1104 78-79 0.0559999131841451 1104 80-81 0.05417079719287088 1104 82-83 -0.11742866925007078 1104 84-85 -0.19517175308783763 1104 86-87 -0.2097168411567445 1104 88-89 -0.1624525175210536 1104 90-91 0.06391528083559095 1104 92-93 -0.07969958285463008 1104 94-95 -0.09134657203257035 1104 96-97 -0.6548649601083625 1104 98-99 -0.326648292932056 1104 100-101 -0.36043862223417733 1105 1 0.08139910585148868 1105 2 0.046907910179093903 1105 3 0.01184596492385026 1105 4 0.08867224248858463 1105 5 0.20544981402215967 1105 6 0.16254038116542802 1105 7 0.13492616446357886 1105 8 0.271124528435422 1105 9 0.3649587985414371 1105 10-11 0.35422544454981875 1105 12-13 0.2325987977458297 1105 14-15 0.18980989716440178 1105 16-17 0.10443634145655523 1105 18-19 0.010827483635473811 1105 20-21 0.2886365972009912 1105 22-23 0.3162457726717989 1105 24-25 0.435214243603788 1105 26-27 0.18125995457626942 1105 28-29 0.18775177751031435 1105 30-31 0.15591095905417518 1105 32-33 0.27538826114058423 1105 34-35 0.24388875980933022 1105 36-37 0.17670960644391442 1105 38-39 0.29094666014209736 1105 40-41 -0.12669375824863494 1105 42-43 0.11080362584326764 1105 44-45 -0.014494794500301111 1105 46-47 0.3207837798419533 1105 48-49 0.22235650138839702 1105 50-51 0.14693698280431988 1105 52-53 0.36096324431112947 1105 54-55 0.2911661720836989 1105 56-57 0.013523770094593601 1105 58-59 0.20757973494474413 1105 60-61 0.3603066529338008 1105 62-63 0.2656570242750149 1105 64-65 -0.027148162027920364 1105 66-67 0.45461691373357027 1105 68-69 1.8167435282515143 1105 70-71 2.0187476151538952 1105 72-73 0.9211056023640118 1105 74-75 0.5441555896289891 1105 76-77 0.3771900357607336 1105 78-79 0.1595752694768926 1105 80-81 0.1731503536724297 1105 82-83 0.01974403857481377 1105 84-85 0.1959631108731159 1105 86-87 0.2266984621063841 1105 88-89 0.4088436950795078 1105 90-91 0.29850731434066446 1105 92-93 0.2129482070757689 1105 94-95 0.7229537232459862 1105 96-97 0.3937957407262509 1105 98-99 0.5608502005446994 1105 100-101 0.7759864734300486 1106 1 0.5271264265788105 1106 2 0.42841304168422667 1106 3 0.3639645670424514 1106 4 0.4199306987470379 1106 5 0.47258508115742615 1106 6 0.48849820712325 1106 7 0.4815791361165509 1106 8 0.36469199700289323 1106 9 0.47740641098904746 1106 10-11 0.5416120069363757 1106 12-13 0.6255446281916548 1106 14-15 0.670137752492252 1106 16-17 0.7517526137728296 1106 18-19 0.6321067299147174 1106 20-21 0.7414919250563159 1106 22-23 0.8617816932077176 1106 24-25 0.8296987005882457 1106 26-27 0.7896011254174411 1106 28-29 0.6315609713195016 1106 30-31 0.8929027585459721 1106 32-33 0.8622773480296715 1106 34-35 0.7241196775402514 1106 36-37 0.7938473485816573 1106 38-39 0.7997617939572308 1106 40-41 0.662976406421528 1106 42-43 0.5822668473064851 1106 44-45 0.873208155702649 1106 46-47 0.7787706128287866 1106 48-49 0.6731720147039084 1106 50-51 0.6722913206586583 1106 52-53 0.8078806287285119 1106 54-55 0.6704912389087596 1106 56-57 0.6877123817832071 1106 58-59 0.8005470154120289 1106 60-61 0.9781600832872215 1106 62-63 1.1670735506915406 1106 64-65 1.2277688428890876 1106 66-67 1.446919656036311 1106 68-69 3.0111714726794574 1106 70-71 3.3714478428541206 1106 72-73 1.8319890132474193 1106 74-75 1.3927040006773943 1106 76-77 0.955895114465811 1106 78-79 1.092364514766139 1106 80-81 1.053560296582372 1106 82-83 1.0746932185239935 1106 84-85 1.1017438916538964 1106 86-87 1.204388377296297 1106 88-89 1.2095812458170556 1106 90-91 0.9034597942931448 1106 92-93 0.6977331293606888 1106 94-95 1.0692416320338936 1106 96-97 0.946876293806806 1106 98-99 1.1935247707192715 1106 100-101 1.0910398509834245 1107 1 0.15888583916014198 1107 2 0.14680918131324106 1107 3 0.05599627962210718 1107 4 0.14561103059175906 1107 5 0.0021609086784621923 1107 6 0.013336382646357947 1107 7 0.13882903994179685 1107 8 0.04770419028906048 1107 9 -0.17845104966293235 1107 10-11 0.11104390684773335 1107 12-13 0.09666920376829324 1107 14-15 0.2058687642506669 1107 16-17 0.08860887014963481 1107 18-19 0.10844564974679116 1107 20-21 0.16770916291739013 1107 22-23 0.13313457928662586 1107 24-25 0.12596921089985358 1107 26-27 -0.04608639862625097 1107 28-29 0.21417717551105397 1107 30-31 0.25199419982919125 1107 32-33 0.005765899052470047 1107 34-35 0.14212860924781268 1107 36-37 0.05862062705356408 1107 38-39 0.155071058513073 1107 40-41 -0.11433722363182852 1107 42-43 -0.1124933012207876 1107 44-45 -0.230441048973951 1107 46-47 -0.16713736047645256 1107 48-49 -0.03246424401453396 1107 50-51 -0.006301015467926163 1107 52-53 0.020140400919792967 1107 54-55 0.03534271163695024 1107 56-57 0.014225670625258147 1107 58-59 0.20788054945789014 1107 60-61 0.03844283706312979 1107 62-63 -0.02523832312718355 1107 64-65 -0.1948021173531096 1107 66-67 0.9360435458451342 1107 68-69 1.9199768986081729 1107 70-71 1.8639901179306442 1107 72-73 1.1346530539251596 1107 74-75 0.6874127166189901 1107 76-77 0.20972119705901804 1107 78-79 0.29052214100595464 1107 80-81 0.15199306624801778 1107 82-83 0.35124163205870573 1107 84-85 0.0775810301622677 1107 86-87 0.2112335108537593 1107 88-89 0.21565907210036528 1107 90-91 0.048970105899346805 1107 92-93 0.06797875131864117 1107 94-95 0.400164324257954 1107 96-97 0.15106156819755867 1107 98-99 0.5986525576963686 1107 100-101 0.8682593970385888 1108 1 -0.14717605369381914 1108 2 -0.1858963636699542 1108 3 -0.10473588673263023 1108 4 -0.1828612025523242 1108 5 -0.1675293649271694 1108 6 -0.10534575985504091 1108 7 -0.13668485528177143 1108 8 -0.1375222067038493 1108 9 -0.25783678694071455 1108 10-11 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-0.84850690485192 2316 82-83 -0.743715306846557 2316 84-85 -0.8991703136906892 2316 86-87 -0.8706259192369856 2316 88-89 -1.0278138485400632 2316 90-91 -1.2324283681772954 2316 92-93 -1.4880367969155657 2316 94-95 -1.0087788004169163 2316 96-97 -1.0227910992783116 2316 98-99 -1.6122239812320132 2316 100-101 -1.604240747461727 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 10.0 4 24.0 5 42.0 6 124.0 7 280.0 8 562.0 9 1067.0 10 1911.0 11 3084.0 12 4267.0 13 5676.0 14 7378.0 15 9545.0 16 12288.0 17 14361.0 18 12559.0 19 8982.0 20 8504.0 21 8719.0 22 9809.0 23 10579.0 24 11751.0 25 13744.0 26 16065.0 27 19592.0 28 23910.0 29 28562.0 30 33479.0 31 39544.0 32 46979.0 33 56486.0 34 69449.0 35 87853.0 36 107163.0 37 104193.0 38 53360.0 39 2851.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 50.099811879171305 17.13049834046275 14.05298536971135 18.71670441065459 2 13.384634187841648 23.517711256551404 38.665922157224095 24.43173239838285 3 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23.430347826607807 22-23 20.51332426879743 26.75078316392235 25.328988745200686 27.40690382207953 24-25 22.274925801360286 26.459279846421545 29.275443919005934 21.99035043321224 26-27 25.69806702952915 22.828051222429668 30.556518916108566 20.917362831932618 28-29 20.512378190234372 24.040720954484225 34.63814810222646 20.80875275305494 30-31 20.135489261787175 30.082090200388738 30.876696985034485 18.9057235527896 32-33 19.362337695787833 29.503202507617672 28.433463733765084 22.700996062829407 34-35 21.548658386469754 24.513476035448907 29.03725489608822 24.90061068199312 36-37 24.405352219758555 25.314757098298713 30.79576974134695 19.48412094059578 38-39 26.929480457735817 21.768950969124358 31.976277452128333 19.325291121011492 40-41 21.331517747234198 24.71013880119752 35.03913283578599 18.91921061578229 42-43 18.935495592296743 29.666283318603014 30.993810867497878 20.40441022160236 44-45 19.805009485117598 30.7408344505227 27.187033088790773 22.267122975568928 46-47 19.500343528063187 27.660424008354983 23.948942017498947 28.890290446082883 48-49 22.056685501881024 26.242179888787305 26.79914267300169 24.90199193632998 50-51 26.076355373833177 27.597287753523702 26.756755136421685 19.56960173622144 52-53 20.57693114589015 27.77859030995439 30.4053802950291 21.23909824912636 54-55 17.841908580641487 27.029095605841295 31.895424836601304 23.233570976915907 56-57 21.138150976520954 31.591044588315302 24.696587943808478 22.574216491355266 58-59 20.142914311320283 27.866793030650854 26.451787986475377 25.538504671553486 60-61 20.507005197288496 25.123642271279767 28.463301488259972 25.906051043171757 62-63 27.36446014069119 24.173211650150467 26.382233424699546 22.080094784458797 64-65 25.091783208929076 25.281848878325025 29.422441166798347 20.203926745947552 66-67 19.69065250568711 26.77224211243677 31.105242697944963 22.431862683931158 68-69 19.18859055477152 30.788730598156093 28.787217070855455 21.235461776216926 70-71 19.704242692525835 29.673286031250168 25.039349904098707 25.58312137212529 72-73 19.41098495418085 27.10203709337793 24.52139077064142 28.965587181799794 74-75 21.197093097454573 27.422823223344857 26.768716225891897 24.61136745330867 76-77 23.811877747259334 24.260606757838527 28.092209647344173 23.835305847557965 78-79 20.418699824967685 24.12834265601058 32.02288718262663 23.4300703363951 80-81 19.80885077458204 30.036999522606127 28.12017627034989 22.033973432461945 82-83 19.1506604636453 29.502805914792912 26.131695007334493 25.214838614227297 84-85 20.41723719471508 25.05783147829358 26.602659708919884 27.922271618071452 86-87 22.789392726906804 25.69296362495028 28.061343064014526 23.456300584128385 88-89 25.297153412861757 22.38545727244194 29.059758922988088 23.25763039170822 90-91 20.625097334531297 24.543815513626836 31.780712788259958 23.05037436358191 92-93 19.008444656888642 28.758016074678128 29.22511377944219 23.008425488991037 94-95 18.994955956036367 29.970577409349286 26.402518784777467 24.63194784983688 96-97 18.32835992573737 27.416965260832814 24.071372642523922 30.183302170905897 98-99 21.115742163606157 26.14555643623436 25.401292980270995 27.337408419888483 100-101 24.257825206356852 26.30896348626414 26.138654311913196 23.29455699546581 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 304.0 1 195.0 2 131.5 3 185.5 4 159.5 5 145.0 6 274.0 7 462.0 8 605.0 9 638.5 10 577.0 11 521.5 12 505.5 13 552.5 14 639.5 15 680.5 16 754.5 17 860.5 18 958.0 19 1122.5 20 1245.0 21 1317.5 22 1507.5 23 1710.0 24 1973.5 25 2366.5 26 2709.5 27 3121.5 28 3720.0 29 4437.0 30 5266.5 31 6294.5 32 7896.5 33 9987.5 34 11841.5 35 13750.5 36 15908.5 37 17702.5 38 19059.0 39 20062.5 40 20480.5 41 20469.5 42 20732.0 43 21250.5 44 23858.5 45 26577.5 46 33095.5 47 54241.0 48 129412.0 49 128536.0 50 54957.0 51 34268.5 52 22235.5 53 16838.5 54 14185.0 55 12217.5 56 10371.0 57 8236.5 58 6011.0 59 4267.0 60 3021.5 61 2091.0 62 1479.0 63 997.5 64 677.5 65 498.0 66 348.5 67 257.0 68 200.0 69 158.0 70 126.0 71 115.0 72 117.5 73 116.5 74 95.5 75 64.5 76 47.5 77 39.5 78 27.0 79 14.0 80 9.0 81 7.0 82 3.0 83 1.0 84 2.0 85 2.5 86 1.5 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.022162191301639406 2 0.02407892136015957 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 4.19284700301286E-4 18-19 0.0 20-21 0.0 22-23 0.0 24-25 2.994890716437757E-4 26-27 4.19284700301286E-4 28-29 5.989781432875514E-5 30-31 2.994890716437757E-4 32-33 0.0022162191301639406 34-35 0.0038933579313690844 36-37 0.10278464938814384 38-39 0.09350048816718678 40-41 0.10356332097441764 42-43 0.104701379446664 44-45 0.0978730286131859 46-47 0.09128426903702284 48-49 0.09194314499463914 50-51 0.09104467777970782 52-53 0.07187737719450617 54-55 0.06259321597354912 56-57 0.034441243239034205 58-59 0.015453636096818827 60-61 0.010661810950518415 62-63 0.0022761169444926958 64-65 0.005211109846601697 66-67 0.15465615659684578 68-69 0.42934753310851687 70-71 0.41107869973824657 72-73 0.183706596546292 74-75 0.030967170007966408 76-77 0.03438134542470545 78-79 0.0062293726901905344 80-81 0.001737036615533899 82-83 5.989781432875514E-5 84-85 2.3959125731502057E-4 86-87 8.38569400602572E-4 88-89 1.1979562865751029E-4 90-91 5.989781432875514E-4 92-93 0.0031146863450952672 94-95 0.08409653131757222 96-97 0.01790944648429779 98-99 0.020544950314763014 100-101 0.011560278165449743 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 834755.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 39.79665330174 #Duplication Level Percentage of deduplicated Percentage of total 1 76.94887812312795 30.623078246239686 2 13.268086281443187 10.560508594403343 3 4.605012771286003 5.497922901268621 4 1.9994294726851523 3.182824061029273 5 1.050840052970807 2.090995863183065 6 0.5829995322481415 1.3920858155973523 7 0.3698102287973536 1.0302046624019796 8 0.2198806867876361 0.7000412367868828 9 0.1566175650443149 0.5609569443328183 >10 0.6645710595845595 4.570559786507216 >50 0.054574858290358255 1.5300471842798835 >100 0.054273331529319555 4.603220395457919 >500 0.009347073763382197 2.5553980903411886 >1k 0.011457703322855596 9.833890857331491 >5k 0.002110629559473399 5.525496396695672 >10k+ 0.002110629559473399 15.742768964143622 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 30764 3.6853927200196464 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 29726 3.561044857473151 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 23021 2.7578151673245443 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 14111 1.6904361159861276 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 12444 1.490736803014058 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 10634 1.2739067151439643 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 10496 1.257374918389228 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 9269 1.1103856820264628 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 8125 0.973339482842271 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 6248 0.7484830878521243 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 5853 0.7011638145324077 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 5675 0.6798401926313709 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 5616 0.6727722505405778 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 5262 0.6303645979958191 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4936 0.5913112230534707 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 4416 0.5290174961515655 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4347 0.5207515977741972 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 4252 0.5093710130517338 No Hit GCTTATACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 4127 0.49439655946954497 No Hit GATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTT 4017 0.48121904031721885 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 3077 0.3686111493791592 No Hit GTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGC 2875 0.3444124323903421 No Hit TGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGT 2758 0.3303963438374134 No Hit CCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATA 2705 0.3240471755185653 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 2607 0.31230720391012934 No Hit AGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAG 2573 0.30823415253577396 No Hit ATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGT 2504 0.2999682541584058 No Hit AAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTAC 2324 0.27840504100005387 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2110 0.2527687764673467 No Hit GCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAAC 2065 0.24737797317775875 No Hit GTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTT 1979 0.23707554911321285 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1871 0.22413762121820174 No Hit GCTTATACTCTGCGTTGATACCACTGCTTATACTCTGCGTTGATACCACT 1802 0.21587172284083353 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 1734 0.20772562009212284 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 1563 0.18724056759168858 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 1550 0.18568322441914092 No Hit TACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGA 1544 0.18496445064719588 No Hit CTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACT 1508 0.1806518080155255 No Hit GTATAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCAGA 1443 0.17286509215278736 No Hit GCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAA 1327 0.15896879922851617 No Hit TCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTA 1277 0.15297901779564063 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 1262 0.15118208336577799 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 1260 0.15094249210846297 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 1233 0.1477080101347102 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1229 0.14722882762008013 No Hit GTATAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1181 0.14147863744451966 No Hit GAGTATAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCA 1179 0.14123904618720465 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 1090 0.1305772352366862 No Hit GTACATGGGACTCCCGACCCGGGGAGGTAGTGACGAAAAAAAAAAAAAAA 1086 0.13009805272205616 No Hit TTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCG 1055 0.12638438823367334 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 1045 0.12518643194709825 No Hit CATGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 1042 0.12482704506112571 No Hit AAAAAGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 999 0.11967583302885276 No Hit GAGTATAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 954 0.11428502973926483 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 896 0.10733688327712922 No Hit GCTTCCCATGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATA 885 0.10601913136189661 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 842 0.10086791932962366 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 1.1979562865751029E-4 0.0 10-11 0.0 0.0 0.0 3.5938688597253086E-4 0.0 12-13 0.0 0.0 0.0 3.5938688597253086E-4 0.0 14-15 0.0 0.0 0.0 4.19284700301286E-4 0.0 16-17 0.0 0.0 0.0 7.187737719450617E-4 0.0 18-19 0.0 0.0 0.0 0.0010781606579175927 0.0 20-21 0.0 0.0 0.0 0.002036525687177675 0.0 22-23 0.0 0.0 0.0 0.00419284700301286 0.0 24-25 0.0 0.0 0.0 0.009104467777970783 0.0 26-27 0.0 0.0 0.0 0.015992716425777626 0.0 28-29 0.0 0.0 0.0 0.027073812076597325 0.0 30-31 0.0 0.0 0.0 0.062233829087576596 0.0 32-33 0.0 0.0 0.0 0.11332666471000474 0.0 34-35 0.0 0.0 0.0 0.20784541572078036 0.0 36-37 0.0 0.0 0.0 0.35046211163754637 0.0 38-39 0.0 0.0 0.0 0.5453097016489868 0.0 40-41 0.0 0.0 0.0 0.8251522901929309 0.0 42-43 0.0 0.0 0.0 1.0634856934070476 0.0 44-45 0.0 0.0 0.0 1.326317302681625 0.0 46-47 0.0 0.0 0.0 1.65952884379249 0.0 48-49 0.0 0.0 0.0 2.0681517331432575 0.0 50-51 0.0 0.0 0.0 2.45874538038107 0.0 52-53 0.0 0.0 0.0 2.7951315056513586 0.0 54-55 0.0 0.0 0.0 3.1247491779024985 0.0 56-57 0.0 0.0 0.0 3.4816203556732215 0.0 58-59 0.0 0.0 0.0 3.982545776904601 0.0 60-61 0.0 0.0 0.0 4.671789926385586 0.0 62-63 0.0 0.0 0.0 5.204281495768219 0.0 64-65 0.0 0.0 0.0 5.586010266485376 0.0 66-67 0.0 0.0 0.0 6.00876904001773 0.0 68-69 0.0 0.0 0.0 6.482860240429827 0.0 70-71 0.0 0.0 0.0 7.083455624704255 0.0 72-73 0.0 0.0 0.0 7.796898491174057 0.0 74-75 0.0 0.0 0.0 8.444274068439242 0.0 76-77 0.0 0.0 0.0 8.996531916550365 0.0 78-79 0.0 0.0 0.0 9.622464076285857 0.0 80-81 0.0 0.0 0.0 10.319135554743607 0.0 82-83 0.0 0.0 0.0 11.041922480248695 0.0 84-85 0.0 0.0 0.0 12.046648417799235 0.0 86-87 0.0 0.0 0.0 13.099172811184118 0.0 88-89 0.0 0.0 0.0 13.819743517559044 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCGACCG 20 5.4245983E-4 47.4364 18-19 GTTCGAC 30 8.359563E-5 39.52915 16-17 TTCGACC 30 8.359563E-5 39.52915 16-17 ACGAAGT 25 0.0016264576 37.950256 82-83 CGACCGT 25 0.0016266969 37.949123 18-19 AAAGTAC 590 0.0 32.96329 3 AAAAGTA 600 0.0 31.632793 2 AAGTACT 650 0.0 30.650295 4 TAGCCCT 920 0.0 30.42026 4 AGTACTC 695 0.0 30.030779 5 GTGTAGC 540 0.0 29.884367 1 GTAGCCC 575 0.0 29.698421 3 GTATAGC 650 0.0 29.208252 1 GTATCGA 90 1.9826984E-10 29.189106 62-63 ATAGCCC 565 0.0 27.705385 3 TACGCTT 35 0.008096907 27.343185 66-67 CGTTTAG 55 9.0168505E-5 26.100313 64-65 TGCGTGG 120 7.2759576E-12 25.872164 62-63 AGCCCTG 1065 0.0 25.833136 5 TATAGCC 625 0.0 25.81236 2 >>END_MODULE