FastQCFastQC Report
Fri 27 May 2016
SRR939761_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939761_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2320257
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63730.27466784929428073No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49510.213381534890316No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31970.13778646072396292No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA91550.034.2547571
GGTATCA55050.033.6974641
ATCAACG111000.028.9803243
TATCAAC111900.028.873192
TCAACGC112650.028.7243164
CAACGCA113600.028.4005745
AACGCAG116600.027.7105466
ACGCAGA127050.025.0952267
GTACATG85450.024.4297121
CGCAGAG130000.024.3422248
TACATGG87750.024.330062
ACATGGG91350.022.748993
GCAGAGT134300.022.2915619
GAGTACT65950.021.86985612-13
AGAGTAC107550.021.3509610-11
AGTACTT68900.020.93348112-13
GTACTTT72250.020.12768614-15
CATGGGG46000.020.1128354
GAGTACA65800.018.5304071
CAGAGTA131200.018.15316410-11
Produced by FastQC (version 0.11.5)