FastQCFastQC Report
Fri 27 May 2016
SRR939761_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939761_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2320257
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70960.3058281905840603No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60790.26199683914325006No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41750.17993696387943234No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA58500.037.825891
GTATCAA90750.033.6379741
ATCAACG110050.027.21723
TCAACGC110850.027.0184444
TATCAAC112200.026.696812
CAACGCA112350.026.5737555
AACGCAG115250.025.9723766
ACGCAGA131450.022.6280777
CGCAGAG131550.022.5750458
GTACATG88350.021.3611971
GAGTACT71200.021.05358512-13
TACATGG88400.020.9176222
GCAGAGT140700.020.7719149
ACATGGG92550.019.6220363
GTACTTT76600.019.5386214-15
AGAGTAC117950.019.32312810-11
AGTACTT73350.018.95836312-13
CAGAGTA139400.018.02367610-11
GAGTACA73200.017.4030281
CGCTATA1650.001534110517.1532272
Produced by FastQC (version 0.11.5)