##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939761_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2320257 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.400229371142938 31.0 31.0 34.0 30.0 34.0 2 31.616919160248198 31.0 31.0 34.0 30.0 34.0 3 31.675593695008786 31.0 31.0 34.0 30.0 34.0 4 35.483645992663746 37.0 35.0 37.0 33.0 37.0 5 35.210324114957956 37.0 35.0 37.0 32.0 37.0 6 35.23808569481743 37.0 35.0 37.0 32.0 37.0 7 35.159839190227636 37.0 35.0 37.0 32.0 37.0 8 35.099272623679184 37.0 35.0 37.0 32.0 37.0 9 36.70226143052257 39.0 35.0 39.0 32.0 39.0 10-11 36.55808128151321 38.0 35.0 39.0 32.0 39.0 12-13 36.494854233819794 38.0 35.0 39.0 32.0 39.0 14-15 37.53669184060214 39.0 36.0 41.0 32.0 41.0 16-17 37.458309575189304 39.0 36.0 41.0 32.0 41.0 18-19 37.43417388677203 39.0 36.0 41.0 32.0 41.0 20-21 37.35748669220694 39.0 36.0 41.0 31.5 41.0 22-23 37.2103560079767 39.0 36.0 40.0 31.0 41.0 24-25 37.0456414957481 39.0 36.0 40.0 31.0 41.0 26-27 36.74404667241603 39.0 35.5 40.0 30.0 41.0 28-29 36.51811976000934 38.0 35.0 40.0 30.0 41.0 30-31 36.26978821742591 38.0 35.0 40.0 30.0 41.0 32-33 36.238164996377556 38.0 35.0 40.0 30.0 41.0 34-35 36.32052289897197 38.0 35.0 40.0 30.0 41.0 36-37 36.28973449923866 38.0 35.0 40.0 30.0 41.0 38-39 36.15853523984627 38.0 35.0 40.0 30.0 41.0 40-41 35.986802324052896 38.0 35.0 40.0 29.0 41.0 42-43 35.78932118295516 38.0 35.0 40.0 28.5 41.0 44-45 35.54856444781763 38.0 34.0 40.0 27.5 41.0 46-47 35.252909914720654 38.0 34.0 40.0 27.0 41.0 48-49 34.95076364385497 38.0 33.5 40.0 26.0 41.0 50-51 34.680750882337605 38.0 33.0 40.0 25.5 41.0 52-53 34.399814977392595 38.0 33.0 40.0 24.0 41.0 54-55 34.08236458288888 37.5 33.0 40.0 24.0 41.0 56-57 32.6547072156231 36.5 31.5 39.5 16.0 41.0 58-59 28.982159303904695 34.0 23.0 38.5 7.0 40.0 60-61 29.010699245816305 34.0 23.5 38.0 8.0 40.0 62-63 31.05217633219079 35.0 29.0 38.0 8.0 40.0 64-65 30.57356491112838 35.0 29.0 38.0 3.5 40.0 66-67 24.91045000618466 28.0 17.5 36.0 2.0 39.0 68-69 17.1297608411482 13.0 7.0 29.0 2.0 35.5 70-71 16.005729753212684 12.5 7.5 25.5 2.0 33.0 72-73 17.987520994441564 16.5 7.0 29.0 2.0 34.0 74-75 18.711724175382294 17.5 7.5 30.0 2.0 35.0 76-77 19.774091404529756 22.5 7.5 30.5 2.0 34.5 78-79 22.856889129092167 27.5 11.0 33.5 2.0 35.5 80-81 25.58553815374762 31.5 20.5 34.5 2.0 36.0 82-83 26.69278920395456 32.5 25.5 35.0 2.0 36.0 84-85 26.71778557289128 33.0 25.5 35.0 2.0 36.0 86-87 26.42674130495027 33.0 25.0 35.0 2.0 35.5 88-89 25.97939129156813 33.0 23.5 35.0 2.0 35.0 90-91 24.207190841359385 31.5 11.0 34.0 2.0 35.0 92-93 21.89797897388091 30.5 2.0 34.0 2.0 35.0 94-95 17.352985251202774 19.5 2.0 33.0 2.0 35.0 96-97 15.969712406858378 10.0 2.0 31.5 2.0 34.5 98-99 14.841675297176131 2.0 2.0 31.0 2.0 34.0 100-101 14.227915916211007 2.0 2.0 31.5 2.0 34.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.44587758377759457 1101 2 0.46564307293512996 1101 3 0.45335110287174984 1101 4 0.3535177470325479 1101 5 0.4426968628904149 1101 6 0.3891504194861355 1101 7 0.43148254525564766 1101 8 0.5022714432404882 1101 9 0.6256270225100948 1101 10-11 0.456340559482328 1101 12-13 0.5704778108567226 1101 14-15 0.5598164839158386 1101 16-17 0.6568445938179153 1101 18-19 0.6417666069087886 1101 20-21 0.6534641050239856 1101 22-23 0.7149000905780127 1101 24-25 0.6726046260300151 1101 26-27 0.8215577688569269 1101 28-29 0.8226698732057827 1101 30-31 0.7223881238332126 1101 32-33 0.599120346736882 1101 34-35 0.8930204744386998 1101 36-37 0.7823527060779085 1101 38-39 0.784018314604431 1101 40-41 0.8072236793991863 1101 42-43 0.9078264993234839 1101 44-45 0.7816339982460292 1101 46-47 0.7332158138145672 1101 48-49 0.5794606095560297 1101 50-51 0.6498983002289904 1101 52-53 0.6813970274355725 1101 54-55 0.6811994507975072 1101 56-57 1.23574504449914 1101 58-59 -8.063895912083161 1101 60-61 -9.768887290832115 1101 62-63 -2.5424764920329643 1101 64-65 0.033881846577106245 1101 66-67 -0.9220688503161902 1101 68-69 -3.111427521302403 1101 70-71 -3.8725284393209556 1101 72-73 -6.615101921761063 1101 74-75 -7.1008589597733405 1101 76-77 -8.15213704628969 1101 78-79 -11.38505326217619 1101 80-81 -7.60804130714633 1101 82-83 -3.500665562642677 1101 84-85 -2.529412995721298 1101 86-87 -2.40005029838818 1101 88-89 -2.329857277668726 1101 90-91 -8.128799505529724 1101 92-93 -13.984711233195801 1101 94-95 -12.348956445513657 1101 96-97 -12.32620421050756 1101 98-99 -11.937699171650959 1101 100-101 -11.667999787222783 1102 1 0.5287628198661736 1102 2 0.5234739761699316 1102 3 0.3767987477678574 1102 4 0.37115934406132567 1102 5 0.48082849294404895 1102 6 0.49536052012520315 1102 7 0.4675445952452719 1102 8 0.40649937253805035 1102 9 0.5853441855698804 1102 10-11 0.687812121037453 1102 12-13 0.673089529067326 1102 14-15 0.8306474789808149 1102 16-17 0.824380246033229 1102 18-19 0.7817447037113965 1102 20-21 0.8499925842822691 1102 22-23 0.8353957354469159 1102 24-25 0.7832472331907283 1102 26-27 0.9777745016753272 1102 28-29 0.8868192026754471 1102 30-31 0.9541802407228275 1102 32-33 0.7713452898531941 1102 34-35 0.8434355808426659 1102 36-37 1.0220269130223087 1102 38-39 0.849150567062587 1102 40-41 0.8339258537336676 1102 42-43 0.9150776820621331 1102 44-45 0.8643466276160368 1102 46-47 0.844712101494693 1102 48-49 0.8449722566832776 1102 50-51 0.7626759589676482 1102 52-53 0.7827112192790153 1102 54-55 0.7147871877278575 1102 56-57 1.3780461046274937 1102 58-59 -8.255724046808233 1102 60-61 -9.47526115528397 1102 62-63 -2.5453368319406557 1102 64-65 0.32047702175167103 1102 66-67 -1.0234791237320344 1102 68-69 -3.6974766470451215 1102 70-71 -3.82873564901006 1102 72-73 -6.548644933994883 1102 74-75 -7.397412607301916 1102 76-77 -8.291139872618421 1102 78-79 -11.522343625789672 1102 80-81 -7.418080385987636 1102 82-83 -3.135203571832495 1102 84-85 -2.1444319909631346 1102 86-87 -1.879363850821509 1102 88-89 -2.00754781677988 1102 90-91 -7.7114466633857965 1102 92-93 -13.747118510227033 1102 94-95 -12.058329245415706 1102 96-97 -11.975844882432355 1102 98-99 -11.579760452988005 1102 100-101 -11.277776809952453 1103 1 0.2229493662968487 1103 2 0.27287406071086195 1103 3 0.2571599615444171 1103 4 0.18555406350466086 1103 5 0.2318670096481128 1103 6 0.20005723856416324 1103 7 0.29030344765631355 1103 8 0.2880878749713105 1103 9 0.30275961754152547 1103 10-11 0.2709453019080783 1103 12-13 0.27913456698429684 1103 14-15 0.3711765462486696 1103 16-17 0.3572054305600716 1103 18-19 0.39226878523197684 1103 20-21 0.4235666786285037 1103 22-23 0.3430577090080007 1103 24-25 0.4335237976495705 1103 26-27 0.3022996815054526 1103 28-29 0.48207443319400056 1103 30-31 0.40928611559501604 1103 32-33 0.3511385619846763 1103 34-35 0.360889851062943 1103 36-37 0.2741337525282148 1103 38-39 0.30938164560978265 1103 40-41 0.3097222184735955 1103 42-43 0.46019201102752305 1103 44-45 0.30847464570924643 1103 46-47 0.3338567206811547 1103 48-49 0.2598877699442781 1103 50-51 0.19824709799412688 1103 52-53 0.3244829298599967 1103 54-55 0.18862285316254912 1103 56-57 0.8686586289691434 1103 58-59 -6.492646214957979 1103 60-61 -8.858593585402222 1103 62-63 -2.71170417312835 1103 64-65 -0.4228129933862128 1103 66-67 -2.061282468518133 1103 68-69 -4.2434548109172425 1103 70-71 -4.803815764188046 1103 72-73 -7.475689000052421 1103 74-75 -8.086927905959017 1103 76-77 -9.182888460893246 1103 78-79 -12.211889633759643 1103 80-81 -8.510465018324904 1103 82-83 -4.7467811634275385 1103 84-85 -3.754685107958391 1103 86-87 -3.502349771504772 1103 88-89 -3.643315134122691 1103 90-91 -8.977029291308149 1103 92-93 -14.533557458306618 1103 94-95 -12.471631435892927 1103 96-97 -12.248757100764731 1103 98-99 -11.880824982286496 1103 100-101 -11.497596239192777 1104 1 0.14744701705434338 1104 2 0.18210276500457567 1104 3 0.24783575101493227 1104 4 0.2178228186546889 1104 5 0.07983614637116432 1104 6 0.2687626771471727 1104 7 0.23506514152116864 1104 8 0.2931079779794601 1104 9 0.2691542270129119 1104 10-11 0.24396079832207818 1104 12-13 0.23092779447743794 1104 14-15 0.16960980712379836 1104 16-17 0.3568207694038392 1104 18-19 0.34902411904435127 1104 20-21 0.2692582025253998 1104 22-23 0.2557440704463829 1104 24-25 0.3791622960023133 1104 26-27 0.35147974390085324 1104 28-29 0.2897251756269412 1104 30-31 0.21655114238647144 1104 32-33 0.2665091909549986 1104 34-35 0.3064485098179546 1104 36-37 0.19006288208076683 1104 38-39 0.31197477548771957 1104 40-41 0.3634492966004075 1104 42-43 0.20980094763940116 1104 44-45 0.1689221843147024 1104 46-47 0.18631894374696145 1104 48-49 0.1714154027303394 1104 50-51 0.2744329796417091 1104 52-53 0.22087854862441247 1104 54-55 0.1287559593296308 1104 56-57 0.7086373283670326 1104 58-59 -6.609780244552862 1104 60-61 -8.802018116645865 1104 62-63 -2.760894253133653 1104 64-65 -0.4745460398995611 1104 66-67 -1.9728669681874784 1104 68-69 -4.4818227087707605 1104 70-71 -4.965495770093165 1104 72-73 -7.886365211317035 1104 74-75 -8.702209506245836 1104 76-77 -9.279999698542586 1104 78-79 -12.17791487211097 1104 80-81 -8.720715895430672 1104 82-83 -5.002005685934019 1104 84-85 -4.169306977078691 1104 86-87 -3.992131658762222 1104 88-89 -4.324154308551126 1104 90-91 -9.74167511230777 1104 92-93 -15.049811444637475 1104 94-95 -12.623867848473841 1104 96-97 -12.448438344936818 1104 98-99 -11.75032015500365 1104 100-101 -11.299540333397477 1105 1 0.1278489457489549 1105 2 0.11116199845405106 1105 3 0.1530425225631653 1105 4 0.08220461571941229 1105 5 0.1218155220090722 1105 6 0.09841670875242414 1105 7 0.14990412367722428 1105 8 0.07224147321051788 1105 9 0.14581350269657634 1105 10-11 0.16577334891510986 1105 12-13 0.22992614030505365 1105 14-15 0.26071669481604687 1105 16-17 0.1673807243540466 1105 18-19 0.27012713526931265 1105 20-21 0.3117091932690741 1105 22-23 0.28018147585940056 1105 24-25 0.30634421976960624 1105 26-27 0.15560039289955085 1105 28-29 0.22462680516271405 1105 30-31 0.3109262523713383 1105 32-33 0.26427852189506496 1105 34-35 0.282508763926991 1105 36-37 0.21768404140924247 1105 38-39 0.25822965881577176 1105 40-41 0.3091994813749892 1105 42-43 0.2847673362643235 1105 44-45 0.23640256301460028 1105 46-47 0.14919649979525218 1105 48-49 0.336027376122793 1105 50-51 0.22125028158096427 1105 52-53 0.2794622955008421 1105 54-55 0.11747872707677942 1105 56-57 0.7236804424345351 1105 58-59 -6.916999475186728 1105 60-61 -8.719829681774506 1105 62-63 -2.644881864438336 1105 64-65 -0.2759749632797046 1105 66-67 -1.6707102089575478 1105 68-69 -4.190777060651943 1105 70-71 -4.734729571522088 1105 72-73 -6.233784350443491 1105 74-75 -6.70113535004973 1105 76-77 -8.513653307805953 1105 78-79 -11.900433437556366 1105 80-81 -7.84905362815865 1105 82-83 -3.955785997763112 1105 84-85 -3.0894329757412784 1105 86-87 -2.886448344786224 1105 88-89 -3.095009902821353 1105 90-91 -8.593420440150657 1105 92-93 -11.679929348413918 1105 94-95 -11.842353048910262 1105 96-97 -12.146448410751761 1105 98-99 -11.537093111044896 1105 100-101 -11.09419137341437 1106 1 0.13283365371238887 1106 2 0.17512271858498352 1106 3 0.16924100493186245 1106 4 0.08728752335551349 1106 5 0.1267952703930817 1106 6 0.055882033026257716 1106 7 0.20317480099046037 1106 8 0.21598562503977092 1106 9 0.1554960896132087 1106 10-11 0.16403741345577316 1106 12-13 0.23027959299892586 1106 14-15 0.3017760609818012 1106 16-17 0.08233104590011209 1106 18-19 0.23012358090405627 1106 20-21 0.1726201853502758 1106 22-23 0.3257286827895811 1106 24-25 0.2775652682246985 1106 26-27 0.3309555814462328 1106 28-29 0.27398569686202734 1106 30-31 0.11780742084825846 1106 32-33 0.26772567757625865 1106 34-35 0.14528712032236513 1106 36-37 0.1836464477971802 1106 38-39 0.17117961665935155 1106 40-41 0.4357795207209847 1106 42-43 0.2728344228420454 1106 44-45 0.16660334332176063 1106 46-47 0.22954631546421922 1106 48-49 0.37484873207181835 1106 50-51 0.20752241370416158 1106 52-53 0.27652796033246574 1106 54-55 0.16423532745040603 1106 56-57 0.8134279998416716 1106 58-59 -6.464722036313546 1106 60-61 -8.841296560049894 1106 62-63 -2.496442148126281 1106 64-65 -0.09129675732490128 1106 66-67 -1.4753902334248075 1106 68-69 -3.7544632680615546 1106 70-71 -4.630926070059013 1106 72-73 -5.827575618277313 1106 74-75 -6.299982826450398 1106 76-77 -8.32900361943286 1106 78-79 -11.612961583169618 1106 80-81 -7.655787001168619 1106 82-83 -3.845205478991101 1106 84-85 -2.8134159313199802 1106 86-87 -2.639642561916613 1106 88-89 -2.869267040482743 1106 90-91 -8.020838317781301 1106 92-93 -11.209089691616812 1106 94-95 -11.585833929731567 1106 96-97 -12.079980871199115 1106 98-99 -11.54544603471697 1106 100-101 -11.236041379200547 1107 1 0.015820486090436958 1107 2 0.1331108010964961 1107 3 -0.022535887466109017 1107 4 -0.03390311726693085 1107 5 -0.09891598797099022 1107 6 0.07660586705718941 1107 7 -0.019081020914853752 1107 8 0.009103403829669787 1107 9 -0.02971185826230993 1107 10-11 0.03429231228956553 1107 12-13 0.04711645801560138 1107 14-15 0.09024976290402975 1107 16-17 0.014086260308133092 1107 18-19 -0.03100683031551199 1107 20-21 0.21516172018980484 1107 22-23 0.17111166962195767 1107 24-25 0.12132257474795693 1107 26-27 0.06255023817309535 1107 28-29 0.040149246754523915 1107 30-31 0.25944162909481605 1107 32-33 0.22789186423672447 1107 34-35 0.1462564805648796 1107 36-37 0.07882309503384732 1107 38-39 0.19792537735542481 1107 40-41 -0.014437799776750637 1107 42-43 0.09834856556809513 1107 44-45 0.09349570750658387 1107 46-47 0.12614000587170437 1107 48-49 0.21704556795023677 1107 50-51 0.04760415891750114 1107 52-53 0.022135768463336092 1107 54-55 -0.0036957760052374056 1107 56-57 0.6229330010512584 1107 58-59 -7.109477016912827 1107 60-61 -9.524934070000281 1107 62-63 -3.0860902321784955 1107 64-65 -0.6577677048990331 1107 66-67 -2.03801279718499 1107 68-69 -4.538229203884432 1107 70-71 -5.113836745658164 1107 72-73 -6.439039544831633 1107 74-75 -7.26925302556625 1107 76-77 -9.518320958716377 1107 78-79 -12.685624239047744 1107 80-81 -8.86924205245112 1107 82-83 -5.02983549354671 1107 84-85 -4.101432658781423 1107 86-87 -3.808683412223605 1107 88-89 -3.748935951082661 1107 90-91 -8.799812019027787 1107 92-93 -12.016577247720775 1107 94-95 -11.902694614858763 1107 96-97 -12.602074614990682 1107 98-99 -11.834134624444793 1107 100-101 -11.368113417972253 1108 1 0.016678364789783018 1108 2 0.04134149586131031 1108 3 0.06592064563674072 1108 4 0.059075518605475 1108 5 -0.04910533393771033 1108 6 0.0711453513813467 1108 7 0.08708397967638604 1108 8 0.07484138537564178 1108 9 0.051480785818988295 1108 10-11 0.08082107387109261 1108 12-13 -0.0567053924134342 1108 14-15 0.06261755422787729 1108 16-17 0.11143498857583722 1108 18-19 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1.8370786753488844 2312 84-85 1.4870529053282802 2312 86-87 1.4083881487559573 2312 88-89 1.5190443245304834 2312 90-91 2.8318534029797355 2312 92-93 4.953369509913795 2312 94-95 9.025931444831777 2312 96-97 9.777206439481821 2312 98-99 10.02898123505418 2312 100-101 9.025541237109666 2313 1 -0.21701893839045283 2313 2 -0.2330101869764114 2313 3 -0.28706434077217224 2313 4 -0.3138387651087342 2313 5 -0.35775535229689837 2313 6 -0.25625283436082924 2313 7 -0.373482724941411 2313 8 -0.3205120554536123 2313 9 -0.442412967301415 2313 10-11 -0.3928521444289288 2313 12-13 -0.37745654415545005 2313 14-15 -0.5561948042808851 2313 16-17 -0.48414036401307214 2313 18-19 -0.3379607803103184 2313 20-21 -0.46637746804606905 2313 22-23 -0.5299589181468818 2313 24-25 -0.5116007932747451 2313 26-27 -0.5581958476516533 2313 28-29 -0.5611447347396208 2313 30-31 -0.6105176569202513 2313 32-33 -0.38685683832970597 2313 34-35 -0.4198893897956708 2313 36-37 -0.6326295247155755 2313 38-39 -0.7931718563357393 2313 40-41 -0.984109435609426 2313 42-43 -0.5854951394865608 2313 44-45 -0.5053161974376081 2313 46-47 -0.5434556842973279 2313 48-49 -0.584937905835865 2313 50-51 -0.30658836983383964 2313 52-53 -0.34235488290574523 2313 54-55 -0.2577133426268787 2313 56-57 0.6870995674596188 2313 58-59 4.177686456982649 2313 60-61 3.9309121738680126 2313 62-63 1.2961418662459927 2313 64-65 1.3453981218764959 2313 66-67 2.002281128255646 2313 68-69 0.1651658953986299 2313 70-71 3.0110051766216 2313 72-73 8.4820639504462 2313 74-75 10.169406324978699 2313 76-77 8.93396400773355 2313 78-79 6.651869642917244 2313 80-81 3.822714884785359 2313 82-83 2.18717582242553 2313 84-85 1.822926212164269 2313 86-87 1.6306073947281234 2313 88-89 1.6403727314685668 2313 90-91 2.9950429186024365 2313 92-93 4.904930926520862 2313 94-95 8.801974163612318 2313 96-97 9.422572008674285 2313 98-99 10.060990121947276 2313 100-101 9.357979591198648 2314 1 -0.37638971520435405 2314 2 -0.4204383402419687 2314 3 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1.2438113334165912 2314 64-65 1.1595423606796196 2314 66-67 1.7382366687954267 2314 68-69 -0.126067646442106 2314 70-71 2.5697707235539884 2314 72-73 8.342865809068648 2314 74-75 9.769066156976503 2314 76-77 8.794424506749426 2314 78-79 6.485566357173322 2314 80-81 3.408353037994843 2314 82-83 1.9741837248653766 2314 84-85 1.6763024479281015 2314 86-87 1.6567128180701118 2314 88-89 1.6563128933024593 2314 90-91 3.0156813311611046 2314 92-93 4.8930914608643015 2314 94-95 8.812503390162945 2314 96-97 9.551381112251343 2314 98-99 9.510367438566371 2314 100-101 8.727952247382936 2315 1 -0.7969490340490246 2315 2 -0.5936050266355544 2315 3 -0.4748313777300872 2315 4 -0.3109087762649452 2315 5 -0.7013369706595469 2315 6 -0.6292975304134245 2315 7 -0.6184357264368145 2315 8 -0.7720107262029714 2315 9 -0.9298251418130334 2315 10-11 -0.933702864835297 2315 12-13 -0.6845883595320288 2315 14-15 -0.8435102520721856 2315 16-17 -0.9089983893637736 2315 18-19 -0.8217879836619275 2315 20-21 -0.8812950426383921 2315 22-23 -0.8826101500773902 2315 24-25 -0.8860058942740565 2315 26-27 -0.934185367934596 2315 28-29 -0.9490225920350639 2315 30-31 -1.042687302532535 2315 32-33 -1.0769352285122338 2315 34-35 -1.0783253840039322 2315 36-37 -0.9693557109530886 2315 38-39 -1.095927232679827 2315 40-41 -0.9444010399674738 2315 42-43 -0.6951560915623318 2315 44-45 -0.7741578801264879 2315 46-47 -0.7307301864217592 2315 48-49 -0.6818114964741397 2315 50-51 -0.5732544430850481 2315 52-53 -0.5026393662782382 2315 54-55 -0.618930526751825 2315 56-57 0.6082242677848129 2315 58-59 4.164606096660776 2315 60-61 3.70480456390813 2315 62-63 1.318317060292852 2315 64-65 1.223821387323099 2315 66-67 1.7705687092568532 2315 68-69 5.748844151469271 2315 70-71 4.9074771733459475 2315 72-73 8.898725424727573 2315 74-75 10.241065692635186 2315 76-77 8.899009611711808 2315 78-79 6.244348250289843 2315 80-81 3.272029786634441 2315 82-83 1.7155255043548436 2315 84-85 1.1107608984848554 2315 86-87 1.1216520136367158 2315 88-89 1.1823108857091142 2315 90-91 2.4974707296114786 2315 92-93 4.469392906392212 2315 94-95 8.344852881360186 2315 96-97 9.02865911612996 2315 98-99 9.343214806843667 2315 100-101 8.451087218960994 2316 1 -0.4538590432317591 2316 2 -0.43849824029709694 2316 3 -0.5952770366654825 2316 4 -0.38752908492337923 2316 5 -0.565769418303141 2316 6 -0.4423362756369258 2316 7 -0.7201234245321402 2316 8 -0.6325886461650612 2316 9 -0.7702195551546609 2316 10-11 -0.5682762378617454 2316 12-13 -0.7155467706224101 2316 14-15 -0.7568315145503348 2316 16-17 -0.9552730864815828 2316 18-19 -0.9979169418656753 2316 20-21 -0.8466746451147671 2316 22-23 -0.9640976793288516 2316 24-25 -2.3452260281642765 2316 26-27 -1.6150056677065123 2316 28-29 -1.2598360047546464 2316 30-31 -0.894252810989542 2316 32-33 -0.8372917260388277 2316 34-35 -1.0068851754669481 2316 36-37 -0.8336474553767985 2316 38-39 -1.2742037663449182 2316 40-41 -0.9034267812512766 2316 42-43 -1.0433410200258493 2316 44-45 -0.8418435343224147 2316 46-47 -0.8904912871652613 2316 48-49 -1.123440025162786 2316 50-51 -1.1612198745255498 2316 52-53 -0.9789123601465448 2316 54-55 -0.969977654925053 2316 56-57 0.32582920276511373 2316 58-59 3.7579867507085893 2316 60-61 3.7816303733219137 2316 62-63 1.2940253899234584 2316 64-65 0.8303152111922891 2316 66-67 5.987425695541994 2316 68-69 8.480536070661191 2316 70-71 5.352019739772681 2316 72-73 9.25878681576404 2316 74-75 10.105512951144025 2316 76-77 8.777806747290466 2316 78-79 6.254304854454656 2316 80-81 3.183434893420781 2316 82-83 1.7687594567823446 2316 84-85 1.3826698709070229 2316 86-87 1.2032302594378343 2316 88-89 1.2619322459389757 2316 90-91 2.625743930831895 2316 92-93 4.378950357738518 2316 94-95 8.453523902421235 2316 96-97 9.267169587411692 2316 98-99 9.54485362908366 2316 100-101 8.278636909413914 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 15.0 4 35.0 5 90.0 6 237.0 7 568.0 8 1354.0 9 2308.0 10 3783.0 11 6043.0 12 8184.0 13 11461.0 14 15157.0 15 20763.0 16 28206.0 17 36173.0 18 39978.0 19 41879.0 20 44237.0 21 43988.0 22 42755.0 23 40774.0 24 41912.0 25 44097.0 26 57780.0 27 84428.0 28 118928.0 29 157289.0 30 182617.0 31 192121.0 32 195003.0 33 198724.0 34 198964.0 35 189422.0 36 153685.0 37 90348.0 38 25993.0 39 955.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 41.65494498388853 20.009095709713225 14.866786649567306 23.469172656830942 2 15.804949196899834 21.1436063606671 39.05330467981591 23.99813976261716 3 19.15422467434456 24.901354083563813 30.194593751225668 25.74982749086596 4 13.32188632552342 17.49319148697752 36.86475248216038 32.32016970533868 5 13.575122740555964 37.77390206286938 34.33453572500595 14.316439471568698 6 31.78851816351299 36.77421801732223 17.25667340155569 14.180590417609091 7 28.21053874635439 32.66245937411244 20.95922994737221 18.167771932160964 8 26.115999469023194 35.552474472600395 19.63532409410702 18.6962019642694 9 26.0217294894488 16.691168262826057 20.2210789580637 37.06602328966145 10-11 24.968785785367743 26.74246861446814 27.49212694973014 20.79661865043398 12-13 25.792013557118885 23.946140449096802 27.94875740058106 22.313088593203254 14-15 22.52620513901298 25.425029447245144 26.096357678544262 25.952407735197614 16-17 22.168664936685893 28.763688677590455 26.661550854064874 22.406095531658778 18-19 22.1307585885882 27.285010537748413 28.919870012886655 21.664360860776732 20-21 22.870354447804704 26.42233166412169 28.806334815496733 21.900979072576874 22-23 22.531038587535775 26.43722225598285 28.680680631499012 22.351058524982363 24-25 22.451306040667045 26.64551814734316 28.773127287192757 22.13004852479704 26-27 22.24553384122302 26.894269572583525 28.675173348440868 22.18502323775258 28-29 22.093649450385342 27.054781310278585 28.779254004960315 22.072315234375758 30-31 22.25212298530828 26.70065109767672 28.68476200410158 22.362463912913423 32-33 22.17959749587275 26.775030012936107 28.61080241586088 22.43457007533026 34-35 22.67113805860793 26.80335414734389 28.404831930600043 22.120675863448138 36-37 22.533054807639868 26.51735033860444 28.571631808921776 22.377963044833916 38-39 22.240462765652463 26.834040417844484 28.680895440156334 22.24460137634672 40-41 22.38900236855878 26.644303172075322 28.64067833730105 22.326016122064846 42-43 22.27335298375585 26.637768732892642 28.437778476191216 22.651099807160293 44-45 22.32198575536836 26.79418617609824 28.332033866190308 22.551794202343093 46-47 22.425563634889965 26.911254073079604 28.05880841457936 22.60437387745107 48-49 22.363852790243385 26.931898479538418 28.016187158141886 22.68806157207631 50-51 22.253044173659625 27.071342195512578 28.014136088411984 22.66147754241581 52-53 22.47655838106582 27.11694491397375 27.69257994248392 22.71391676247651 54-55 22.170825132357923 26.854131696672965 27.972007624644053 23.00303554632506 56-57 22.333796949198994 26.992586102529962 27.974487819563148 22.69912912870789 58-59 20.968895640855596 26.544827253740326 29.627467764932536 22.858809340471538 60-61 22.4100554976142 26.696200547951126 28.271100711688003 22.622643242746673 62-63 22.038067938040204 26.932612547847825 28.043335050823266 22.985984463288705 64-65 21.299983663124564 26.829832558896378 28.57181394780703 23.29836983017203 66-67 21.760111805866266 26.459687091331656 28.967450355436952 22.812750747365122 68-69 22.523551867705134 25.54903419458559 29.404615074214103 22.522798863495176 70-71 21.67105037074241 26.076254422254237 29.48644576322711 22.76624944377624 72-73 21.50607623162302 26.104068162188902 29.46931162100846 22.920543985179613 74-75 21.99222645086366 26.352068878330055 28.661804641143767 22.993900029662516 76-77 20.303052491238528 26.873163207624057 29.219724714862704 23.604059586274705 78-79 21.104352928216063 27.164641556468943 28.100857781576384 23.630147733738607 80-81 21.599753647342464 27.283599920870632 27.444703180819662 23.671943250967246 82-83 21.93339400191315 27.274312128852994 27.014814956880844 23.777478912353008 84-85 21.914602976007178 27.10303796153572 27.056060396770786 23.926298665686314 86-87 21.910752806349493 27.13410784053858 27.141542592508788 23.813596760603144 88-89 21.781755978650633 27.061733705246315 26.984587045567388 24.171923270535665 90-91 21.50256666892012 27.17232719018923 27.277728328996698 24.04737781189396 92-93 21.363894930567174 27.146556423278483 27.384910975945427 24.10463767020892 94-95 20.24640376437217 26.85272234480283 28.57958790499816 24.321285985826837 96-97 19.963164821596934 26.812425465738855 28.77167101038279 24.45273870228143 98-99 20.27831136464924 26.853807067034257 28.0683603704577 24.7995211978588 100-101 19.980404198037043 27.069276439726682 28.045409635785536 24.90490972645074 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 360.0 1 277.0 2 186.0 3 203.5 4 248.0 5 268.0 6 355.5 7 566.5 8 814.5 9 1029.5 10 1168.0 11 1348.5 12 1579.5 13 1795.0 14 2001.0 15 2208.0 16 2407.0 17 2627.0 18 2929.5 19 3226.5 20 3653.0 21 4201.0 22 4961.0 23 6033.5 24 7247.0 25 8959.0 26 11333.5 27 14296.0 28 17562.0 29 21129.0 30 25167.0 31 29553.0 32 34320.0 33 39577.0 34 44705.5 35 49393.5 36 54036.5 37 58816.5 38 63510.5 39 67322.5 40 71284.0 41 76189.0 42 80917.5 43 85080.5 44 88615.5 45 91493.0 46 93972.0 47 96131.0 48 98196.0 49 98325.5 50 96019.0 51 92642.5 52 88513.5 53 84197.5 54 78532.5 55 71316.5 56 63504.0 57 55317.0 58 47053.5 59 39061.0 60 31561.0 61 24979.5 62 19402.5 63 14615.0 64 10907.5 65 8226.0 66 6175.0 67 4542.5 68 3382.0 69 2450.0 70 1746.0 71 1364.0 72 1074.5 73 802.5 74 569.0 75 356.0 76 212.0 77 139.5 78 84.5 79 47.5 80 32.5 81 15.5 82 5.0 83 4.0 84 3.5 85 2.0 86 1.0 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.020773560859853026 2 0.005301136899921 3 0.0038788806584787805 4 0.0 5 0.06658745130388574 6 4.309867398309756E-5 7 0.0 8 2.154933699154878E-4 9 0.0 10-11 0.0 12-13 0.0 14-15 2.585920438985854E-4 16-17 0.0 18-19 0.0011636641975436342 20-21 0.0 22-23 0.0 24-25 0.0 26-27 1.077466849577439E-4 28-29 0.0017454962963154514 30-31 0.007542267947042073 32-33 3.2324005487323175E-4 34-35 0.001077466849577439 36-37 0.028552871513802133 38-39 0.027496954001216243 40-41 0.030298367810117584 42-43 0.03077245322393166 44-45 0.030880199908889402 46-47 0.03271189355317105 48-49 0.03292738692308654 50-51 0.02853132217681059 52-53 0.02404906008256844 54-55 0.0177135550070531 56-57 0.14328154165680784 58-59 0.7636223056325225 60-61 0.30576354257308563 62-63 4.525360768225244E-4 64-65 0.14707422496732042 66-67 1.4102101620639438 68-69 2.699420796920341 70-71 2.1759227533846466 72-73 1.8702023094855442 74-75 1.561443409070633 76-77 1.1134973410273086 78-79 0.43329251888907133 80-81 2.585920438985854E-4 82-83 2.154933699154878E-5 84-85 2.154933699154878E-5 86-87 0.0031031045267830245 88-89 4.309867398309756E-5 90-91 0.22805663338156074 92-93 0.17969992117252528 94-95 1.2995974152863239 96-97 1.2836293565755863 98-99 1.021115333344539 100-101 0.4119371259304465 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2320257.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.16327822217265 #Duplication Level Percentage of deduplicated Percentage of total 1 73.03838183647053 38.829598144695645 2 14.485654875971424 15.402098008032814 3 5.334477388447656 8.507949167157951 4 2.457811833444619 5.226613372766582 5 1.3506443352951187 3.5902340288247916 6 0.8028698123513873 2.5609914726132215 7 0.516608128207365 1.9225207156206792 8 0.3573834370159765 1.5199740075261343 9 0.26468137549004406 1.2664196644864103 >10 1.2610363243716942 11.865300846734375 >50 0.0800775179287838 2.931736144672288 >100 0.04769505525441645 4.617234701381732 >500 0.0020288482961492685 0.7126972322166832 >1k 4.8692359107551747E-4 0.4782090999386049 >5k 1.6230786369183917E-4 0.5684233933319306 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7096 0.3058281905840603 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6079 0.26199683914325006 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4175 0.17993696387943234 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 8.619734796619513E-5 0.0 0.0 0.0 0.0 8 8.619734796619513E-5 0.0 0.0 0.0 0.0 9 8.619734796619513E-5 0.0 0.0 0.0 0.0 10-11 8.619734796619513E-5 0.0 0.0 0.0 0.0 12-13 1.077466849577439E-4 6.464801097464634E-5 0.0 6.464801097464634E-5 0.0 14-15 1.292960219492927E-4 1.077466849577439E-4 0.0 1.292960219492927E-4 0.0 16-17 1.292960219492927E-4 1.292960219492927E-4 0.0 1.7239469593239025E-4 2.154933699154878E-5 18-19 2.154933699154878E-4 1.292960219492927E-4 0.0 2.154933699154878E-4 4.309867398309756E-5 20-21 3.0169071788168294E-4 2.154933699154878E-4 0.0 3.0169071788168294E-4 4.309867398309756E-5 22-23 3.447893918647805E-4 2.154933699154878E-4 0.0 9.266214906365976E-4 4.309867398309756E-5 24-25 6.464801097464634E-4 2.154933699154878E-4 0.0 0.0014007069044506709 4.309867398309756E-5 26-27 6.464801097464634E-4 2.154933699154878E-4 0.0 0.0026505684499605 4.309867398309756E-5 28-29 7.757761316957561E-4 2.154933699154878E-4 0.0 0.008705932144585708 4.309867398309756E-5 30-31 8.619734796619513E-4 2.585920438985854E-4 0.0 0.02618244444473177 4.309867398309756E-5 32-33 8.619734796619513E-4 2.585920438985854E-4 0.0 0.06574702716121533 4.309867398309756E-5 34-35 9.912695016112438E-4 2.585920438985854E-4 0.0 0.1263006641074674 4.309867398309756E-5 36-37 0.001077466849577439 2.8014138089013413E-4 0.0 0.22952198829698606 4.309867398309756E-5 38-39 0.0012714108825013781 3.2324005487323175E-4 0.0 0.3831687610467289 4.309867398309756E-5 40-41 0.0012929602194929268 3.447893918647805E-4 0.0 0.5442500550585561 4.309867398309756E-5 42-43 0.0013145095564844756 3.447893918647805E-4 0.0 0.7123133342556449 4.309867398309756E-5 44-45 0.0014222562414422195 3.8788806584787806E-4 0.0 0.9163209075546372 4.309867398309756E-5 46-47 0.0016377496113577073 3.8788806584787806E-4 0.0 1.1399599268529306 4.309867398309756E-5 48-49 0.0016377496113577073 3.8788806584787806E-4 0.0 1.3602372495805422 4.309867398309756E-5 50-51 0.0016377496113577073 3.8788806584787806E-4 0.0 1.5781656945760751 4.309867398309756E-5 52-53 0.0016808482853408048 4.309867398309756E-4 0.0 1.789112154386346 4.309867398309756E-5 54-55 0.0017239469593239025 6.033814357633658E-4 0.0 2.018828086716256 4.309867398309756E-5 56-57 0.0017239469593239025 6.464801097464634E-4 0.0 2.2181163552140992 4.309867398309756E-5 58-59 0.0017239469593239025 6.89578783729561E-4 0.0 2.350364636331234 4.309867398309756E-5 60-61 0.0017239469593239025 6.89578783729561E-4 0.0 2.4447076336802347 4.309867398309756E-5 62-63 0.001767045633307 7.111281207211098E-4 0.0 2.5269183543029934 4.309867398309756E-5 64-65 0.001767045633307 8.404241426704024E-4 0.0 2.6164558495028785 4.309867398309756E-5 66-67 0.0018101443072900975 8.619734796619513E-4 0.0 2.687633309585964 4.309867398309756E-5 68-69 0.0018101443072900975 8.619734796619513E-4 0.0 2.7716541745160126 4.309867398309756E-5 70-71 0.0018316936442816464 9.050721536450488E-4 0.0 2.9242881284271527 4.309867398309756E-5 72-73 0.0018747923182647441 9.481708276281464E-4 0.0 3.123145410185165 4.309867398309756E-5 74-75 0.0019178909922478414 9.481708276281464E-4 0.0 3.352753595830117 4.309867398309756E-5 76-77 0.0019394403292393903 9.481708276281464E-4 0.0 3.6931253736116303 4.309867398309756E-5 78-79 0.0019394403292393903 9.91269501611244E-4 0.0 4.173158404435371 4.309867398309756E-5 80-81 0.0021118350251717807 0.001077466849577439 0.0 4.714671693695999 4.309867398309756E-5 82-83 0.0021118350251717807 0.001077466849577439 0.0 5.22530478304774 4.309867398309756E-5 84-85 0.002176483036146427 0.001077466849577439 0.0 5.636897119586322 4.309867398309756E-5 86-87 0.002241131047121073 0.001077466849577439 0.0 5.991513009119249 4.309867398309756E-5 88-89 0.002241131047121073 0.0011421148605520854 0.0 6.350180174006587 4.309867398309756E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5850 0.0 37.82589 1 GTATCAA 9075 0.0 33.637974 1 ATCAACG 11005 0.0 27.2172 3 TCAACGC 11085 0.0 27.018444 4 TATCAAC 11220 0.0 26.69681 2 CAACGCA 11235 0.0 26.573755 5 AACGCAG 11525 0.0 25.972376 6 ACGCAGA 13145 0.0 22.628077 7 CGCAGAG 13155 0.0 22.575045 8 GTACATG 8835 0.0 21.361197 1 GAGTACT 7120 0.0 21.053585 12-13 TACATGG 8840 0.0 20.917622 2 GCAGAGT 14070 0.0 20.771914 9 ACATGGG 9255 0.0 19.622036 3 GTACTTT 7660 0.0 19.53862 14-15 AGAGTAC 11795 0.0 19.323128 10-11 AGTACTT 7335 0.0 18.958363 12-13 CAGAGTA 13940 0.0 18.023676 10-11 GAGTACA 7320 0.0 17.403028 1 CGCTATA 165 0.0015341105 17.153227 2 >>END_MODULE