FastQCFastQC Report
Fri 27 May 2016
SRR939760_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939760_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2428761
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67330.27721953703966756No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51210.2108482473162242No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34220.1408948842640342No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA100000.032.74181
GGTATCA63750.032.247391
TATCAAC120300.027.1391412
ATCAACG121500.026.795323
TCAACGC122150.026.7297654
CAACGCA122950.026.173735
AACGCAG125350.025.9733986
GTACATG92350.025.4189111
TACATGG92800.024.7892362
ACATGGG98550.023.0578943
ACGCAGA142150.022.7382437
CGCAGAG144950.022.169638
CAACGCG1805.619462E-620.9100745
GAGTACT68900.020.38255912-13
GCAGAGT149000.019.9566129
GTACCGT1708.5641484E-519.3729676
AGAGTAC116650.019.35971810-11
CATGGGA54800.019.2307644
AGTACTT70950.019.09737612-13
GTACTTT74950.018.773314-15
Produced by FastQC (version 0.11.5)