##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939760_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2428761 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.07543311178004 31.0 30.0 33.0 26.0 34.0 2 30.14398329024552 31.0 30.0 33.0 26.0 34.0 3 30.098254624477253 31.0 30.0 34.0 26.0 34.0 4 33.74579425476611 35.0 33.0 37.0 28.0 37.0 5 33.60582082798596 35.0 33.0 37.0 28.0 37.0 6 33.59743013001279 35.0 33.0 37.0 28.0 37.0 7 33.57632265999001 35.0 33.0 37.0 28.0 37.0 8 33.42440693011787 35.0 33.0 37.0 27.0 37.0 9 34.73282920797888 37.0 34.0 39.0 27.0 39.0 10-11 34.51881617829008 37.0 33.5 39.0 27.0 39.0 12-13 34.41477465259035 37.0 33.0 39.0 27.0 39.0 14-15 35.02209377538588 38.0 33.0 40.0 26.0 41.0 16-17 34.8229560257267 38.0 33.0 40.0 25.0 41.0 18-19 34.84973120039395 38.0 33.5 40.0 25.0 41.0 20-21 34.97666814478658 38.0 34.0 40.0 25.0 41.0 22-23 34.88698599821061 38.0 33.5 40.0 25.0 41.0 24-25 34.69500292535989 38.0 33.0 40.0 25.0 41.0 26-27 34.606132715405096 38.0 33.0 40.0 24.0 41.0 28-29 34.49293281636192 38.0 33.0 40.0 24.0 41.0 30-31 34.30720293186526 38.0 33.0 40.0 23.5 41.0 32-33 34.02413740997982 38.0 33.0 40.0 23.0 41.0 34-35 33.76261785330051 38.0 32.0 40.0 21.0 41.0 36-37 33.54009328213027 38.0 32.0 40.0 19.0 41.0 38-39 33.26338347000796 37.0 31.0 40.0 18.0 41.0 40-41 32.98717267775627 37.0 31.0 40.0 17.0 41.0 42-43 32.67478520941336 37.0 31.0 40.0 15.5 41.0 44-45 32.27742807958461 37.0 30.0 40.0 13.5 41.0 46-47 31.88825145825382 36.5 30.0 39.0 9.0 40.5 48-49 31.5572079755892 36.0 30.0 39.0 8.5 40.0 50-51 30.838380968732615 35.5 28.5 38.5 8.0 40.0 52-53 30.96747168618073 35.5 29.0 39.0 7.0 40.0 54-55 31.04286094844244 36.0 29.0 39.0 4.0 40.0 56-57 30.807838235215403 36.0 28.5 39.0 2.0 40.0 58-59 30.268115718261285 35.0 28.0 39.0 2.0 40.0 60-61 29.76884963156111 34.5 27.0 38.0 2.0 40.0 62-63 29.318162635187242 34.0 26.0 38.0 2.0 40.0 64-65 28.774702204127948 34.0 26.0 38.0 2.0 40.0 66-67 28.225999799897973 33.5 25.5 37.0 2.0 39.0 68-69 27.67991025053515 33.0 24.5 36.5 2.0 39.0 70-71 27.10658932682137 32.5 23.0 36.0 2.0 39.0 72-73 26.60284606019283 32.0 22.0 36.0 2.0 38.5 74-75 26.00560738582347 31.5 20.5 35.0 2.0 38.0 76-77 25.35055548899212 31.0 19.0 35.0 2.0 37.0 78-79 24.67870078612099 31.0 16.5 34.5 2.0 36.0 80-81 23.981992876203133 30.0 9.5 34.0 2.0 36.0 82-83 23.214686624167634 30.0 4.5 34.0 2.0 35.5 84-85 22.453297998444476 29.0 2.0 34.0 2.0 35.0 86-87 21.65401227210088 29.0 2.0 34.0 2.0 35.0 88-89 20.782126359901202 27.5 2.0 33.0 2.0 35.0 90-91 19.812252008328528 26.0 2.0 32.5 2.0 34.0 92-93 18.819530822505797 25.0 2.0 32.0 2.0 34.0 94-95 17.431083997149166 22.0 2.0 32.0 2.0 34.0 96-97 14.854048216353934 2.0 2.0 31.0 2.0 34.0 98-99 13.455503855669619 2.0 2.0 30.0 2.0 34.0 100-101 12.170720997249216 2.0 2.0 28.0 2.0 33.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.8098350179173792 1101 2 0.8942594392300691 1101 3 0.9453298487657555 1101 4 1.1306586042272002 1101 5 1.0675959052278827 1101 6 1.0773461644486275 1101 7 1.0030468028776198 1101 8 0.7787794456832273 1101 9 1.0339285363170063 1101 10-11 1.1195591259271964 1101 12-13 1.0380892255581387 1101 14-15 1.154142657837788 1101 16-17 1.1645347825372312 1101 18-19 1.1787542156661601 1101 20-21 1.194590003944242 1101 22-23 1.0380951899049222 1101 24-25 1.1576823968226506 1101 26-27 1.2918143423055284 1101 28-29 1.4088743378276334 1101 30-31 1.4867846167834031 1101 32-33 1.3550392397349285 1101 34-35 1.3598099950845821 1101 36-37 1.3624918012547553 1101 38-39 1.2437781793623657 1101 40-41 1.1111051979159399 1101 42-43 1.1521155691658649 1101 44-45 1.255091929423287 1101 46-47 1.1066339980696647 1101 48-49 0.9947329691608537 1101 50-51 1.0949479363941883 1101 52-53 1.3117141760875093 1101 54-55 1.36904011158137 1101 56-57 1.1475138206938311 1101 58-59 0.737292896793587 1101 60-61 0.9758064219904554 1101 62-63 0.950756465774603 1101 64-65 1.1559406165757729 1101 66-67 0.8523439235794505 1101 68-69 1.0273655685154743 1101 70-71 0.986750818225989 1101 72-73 0.533115416136976 1101 74-75 0.5743131971961262 1101 76-77 -0.018660129891564736 1101 78-79 0.25730261348460814 1101 80-81 0.1682372215433574 1101 82-83 0.11514939964313342 1101 84-85 0.08718380570361717 1101 86-87 0.1036144547752329 1101 88-89 -0.22239353628599545 1101 90-91 -0.15336115829044772 1101 92-93 -0.2757694782543858 1101 94-95 0.03761637494612202 1101 96-97 1.5730920482896789 1101 98-99 1.6703908807339847 1101 100-101 0.9952423173229565 1102 1 0.8890536518496148 1102 2 0.9279991607463351 1102 3 0.940818870621758 1102 4 0.9217017140944677 1102 5 1.1262116330979808 1102 6 0.9554978432129531 1102 7 1.088885018507625 1102 8 0.7878867902393409 1102 9 1.1609036930502015 1102 10-11 0.9259724927453163 1102 12-13 0.8208834452885583 1102 14-15 1.1182210374840622 1102 16-17 1.2176550600977691 1102 18-19 1.1671303505448236 1102 20-21 1.0350224107239256 1102 22-23 0.9697440196653488 1102 24-25 1.056400186531846 1102 26-27 1.2931598342990185 1102 28-29 1.4935873160037758 1102 30-31 1.2380226360646347 1102 32-33 1.4008498981079711 1102 34-35 1.326920838052601 1102 36-37 1.3139279242675386 1102 38-39 1.2716426276251127 1102 40-41 1.1982731256694947 1102 42-43 1.1128937438655413 1102 44-45 0.9993707311147162 1102 46-47 1.0770855279870482 1102 48-49 0.7612778309623813 1102 50-51 0.9434091262465216 1102 52-53 0.976001720121829 1102 54-55 1.0388475131072425 1102 56-57 0.9912369202555169 1102 58-59 0.7238255889234573 1102 60-61 1.0890303806702022 1102 62-63 0.8354747825609508 1102 64-65 0.6833422869708592 1102 66-67 0.7367418500160561 1102 68-69 0.36605024409388065 1102 70-71 0.6584257009940906 1102 72-73 0.7454055457103514 1102 74-75 0.4514160307740944 1102 76-77 0.29027742621361696 1102 78-79 0.11383048759250869 1102 80-81 0.06815597733453416 1102 82-83 0.31013896649454864 1102 84-85 0.19394295813878415 1102 86-87 -0.05296956322502666 1102 88-89 0.05325218115316588 1102 90-91 -0.2630069208570909 1102 92-93 -0.35726402660428036 1102 94-95 -0.02407532149460323 1102 96-97 1.1538473760973016 1102 98-99 1.1912010634987968 1102 100-101 0.6569500738369101 1103 1 0.758323431646982 1103 2 0.747819834846883 1103 3 0.9210168070667457 1103 4 0.8005111709102479 1103 5 0.9309595377499562 1103 6 0.8181403486650254 1103 7 0.8257902472585528 1103 8 0.6922652164813954 1103 9 0.8611688933240416 1103 10-11 0.7919009411058511 1103 12-13 0.6785423863632829 1103 14-15 0.9916331406898706 1103 16-17 0.8998194370026766 1103 18-19 0.8075521600363516 1103 20-21 0.6542527169634056 1103 22-23 0.8272401923180368 1103 24-25 0.9342134622020382 1103 26-27 1.1159745906891914 1103 28-29 1.0476772418643847 1103 30-31 1.0275584137306453 1103 32-33 1.026982205793459 1103 34-35 1.042901757812352 1103 36-37 0.9028699369094184 1103 38-39 0.9348073361226739 1103 40-41 1.0276876836948858 1103 42-43 0.984459267386832 1103 44-45 1.0052540267469183 1103 46-47 0.9384786159418148 1103 48-49 0.625304754218245 1103 50-51 0.9409386501784276 1103 52-53 0.878284318301688 1103 54-55 0.9156254664051104 1103 56-57 0.8186084539490999 1103 58-59 0.5608591713574675 1103 60-61 0.7590971644651425 1103 62-63 0.41403506485043096 1103 64-65 0.6202580026164988 1103 66-67 0.45178219871268865 1103 68-69 0.30580436333873706 1103 70-71 0.5159377516915811 1103 72-73 0.4348318017929351 1103 74-75 0.34743669734180216 1103 76-77 0.03613477047399627 1103 78-79 -0.09933418397936578 1103 80-81 -0.04006921912857564 1103 82-83 0.10941101352900873 1103 84-85 -0.08141498099439559 1103 86-87 -0.1523350322440038 1103 88-89 0.037890135883749565 1103 90-91 -0.359940611926465 1103 92-93 -0.28904589033225037 1103 94-95 -0.025473623356173647 1103 96-97 1.5460106378465017 1103 98-99 1.5850276796806675 1103 100-101 1.1388422345877132 1104 1 0.5834005986146629 1104 2 0.542865693442554 1104 3 0.6762078587895886 1104 4 0.9031871756263783 1104 5 0.9222422095154528 1104 6 0.991702805702495 1104 7 0.7610332882479369 1104 8 0.7202026762787668 1104 9 1.0004023901909918 1104 10-11 0.9424856731594673 1104 12-13 0.8297199461943592 1104 14-15 1.1016724270297331 1104 16-17 1.0979085517867873 1104 18-19 1.1918479277391185 1104 20-21 0.8320833132061054 1104 22-23 0.767536142542582 1104 24-25 0.8145899427995289 1104 26-27 1.2359777259422415 1104 28-29 1.1561358749234998 1104 30-31 1.1004828541321388 1104 32-33 1.1061232789331683 1104 34-35 1.133459788696804 1104 36-37 1.110358113472735 1104 38-39 0.9461566577746083 1104 40-41 0.9024918039767371 1104 42-43 0.8549702253170395 1104 44-45 1.0198224289895776 1104 46-47 0.9313953727654045 1104 48-49 0.8495036087469252 1104 50-51 0.9548054475542358 1104 52-53 1.2773137882084136 1104 54-55 1.0458553408036337 1104 56-57 0.9500041861778783 1104 58-59 0.9659564598787576 1104 60-61 1.0886645142637086 1104 62-63 0.8635284933305982 1104 64-65 0.7326529238009449 1104 66-67 0.791915719278034 1104 68-69 0.5238872664947429 1104 70-71 0.8577819723015274 1104 72-73 0.7807637741614961 1104 74-75 0.7147969467489759 1104 76-77 0.6229878301424243 1104 78-79 0.811253845241783 1104 80-81 0.5803408366436003 1104 82-83 0.7479732276947608 1104 84-85 0.5344969434180413 1104 86-87 0.5160084060246284 1104 88-89 0.47367345604313726 1104 90-91 0.1201205034287689 1104 92-93 0.32727611929135847 1104 94-95 0.6625354320789292 1104 96-97 1.9366566913283254 1104 98-99 2.1284535839362224 1104 100-101 2.100838772561808 1105 1 0.6521601256122267 1105 2 0.7410429850010551 1105 3 0.697420316627241 1105 4 0.7064964818173962 1105 5 0.8624610794754304 1105 6 0.9571813624581509 1105 7 0.6996369497040433 1105 8 0.7793024366409895 1105 9 0.9538931177417354 1105 10-11 0.9331408989144165 1105 12-13 0.7126470319162479 1105 14-15 0.9107300101930775 1105 16-17 0.8381564111454338 1105 18-19 1.0479929377012525 1105 20-21 0.9036459903057903 1105 22-23 0.710329306747326 1105 24-25 0.9698566868504628 1105 26-27 0.9703026590824777 1105 28-29 0.9374732366743572 1105 30-31 1.1028242827154457 1105 32-33 0.9532297448759763 1105 34-35 1.186144010978957 1105 36-37 1.2319119095988569 1105 38-39 1.06536234231055 1105 40-41 0.9218411880114203 1105 42-43 0.9908754074096677 1105 44-45 1.0036443544796967 1105 46-47 1.0347243121454817 1105 48-49 0.7724849248418586 1105 50-51 0.8399264901745944 1105 52-53 0.7580824706283629 1105 54-55 0.8831830825888822 1105 56-57 0.5940773392621033 1105 58-59 0.6887578100243594 1105 60-61 0.8305173348490307 1105 62-63 0.7103905097333723 1105 64-65 0.7642121613797599 1105 66-67 0.689546756706438 1105 68-69 0.5008117348541212 1105 70-71 0.6532051818794358 1105 72-73 0.7870285733089233 1105 74-75 0.664991466802153 1105 76-77 0.836725240842604 1105 78-79 0.5832398982519855 1105 80-81 0.45750717044702327 1105 82-83 0.39721977064956704 1105 84-85 0.2343091954784846 1105 86-87 0.4496388707935992 1105 88-89 0.6691573473233845 1105 90-91 0.41093808169717505 1105 92-93 0.6007165431068593 1105 94-95 0.8261174702950349 1105 96-97 2.169567648726977 1105 98-99 2.390358543856001 1105 100-101 1.6959935034772187 1106 1 0.8736324881801529 1106 2 0.9139885579515301 1106 3 0.9658602847282189 1106 4 0.9705350997735778 1106 5 0.9889742298750903 1106 6 1.080815917262747 1106 7 1.1587994413252503 1106 8 1.0714103422474253 1106 9 1.2275901988749425 1106 10-11 1.1401063584166948 1106 12-13 1.1141607784355756 1106 14-15 1.2653264901727255 1106 16-17 1.2555146870084144 1106 18-19 1.3725784358320823 1106 20-21 1.412808680633546 1106 22-23 1.051101109492656 1106 24-25 1.2560242600605847 1106 26-27 1.3983046514468427 1106 28-29 1.353888981121834 1106 30-31 1.4021177484491645 1106 32-33 1.5057197366892723 1106 34-35 1.6769846324270716 1106 36-37 1.7598083225481602 1106 38-39 1.6421902438898712 1106 40-41 1.655389610148923 1106 42-43 1.612497396011861 1106 44-45 1.6031893827092816 1106 46-47 1.5253768247236046 1106 48-49 1.5128632223771028 1106 50-51 1.7063003075394256 1106 52-53 1.7263361182183168 1106 54-55 1.5641676603109644 1106 56-57 1.4997427280248559 1106 58-59 1.5975223586558052 1106 60-61 1.6449406486914597 1106 62-63 1.6093700853491626 1106 64-65 1.3908541398611654 1106 66-67 1.4339690637689984 1106 68-69 1.4191582446397568 1106 70-71 1.7408248363419858 1106 72-73 1.7967396937224436 1106 74-75 1.791532767296804 1106 76-77 1.6216065203008476 1106 78-79 1.4058764162773194 1106 80-81 1.5977135205785657 1106 82-83 1.4931658920301238 1106 84-85 1.6926483796158749 1106 86-87 1.7941576167697875 1106 88-89 1.8985571078157548 1106 90-91 2.015613050765186 1106 92-93 1.8837253444824391 1106 94-95 2.3439563510737536 1106 96-97 3.87661192849521 1106 98-99 3.834477955896558 1106 100-101 3.0147363666001983 1107 1 0.6610976299460134 1107 2 0.5653445025689834 1107 3 0.7295621478126684 1107 4 0.2513059685981105 1107 5 0.6742131918864018 1107 6 0.7546350776268937 1107 7 0.6566767511918883 1107 8 0.6122900989722169 1107 9 0.7952660362863782 1107 10-11 0.8635628982011951 1107 12-13 0.8225645290188055 1107 14-15 1.032430761052403 1107 16-17 0.8880658350243493 1107 18-19 0.7775368500943927 1107 20-21 0.6993921939095173 1107 22-23 0.7389134094308076 1107 24-25 0.7577556427795784 1107 26-27 0.9860856874823867 1107 28-29 1.0477912993978649 1107 30-31 1.083549770823538 1107 32-33 1.2349858691342774 1107 34-35 1.2916085432768014 1107 36-37 0.942056868539332 1107 38-39 0.9264710113678234 1107 40-41 1.1040137271640802 1107 42-43 0.9237193737893961 1107 44-45 1.1815793798880705 1107 46-47 1.1574301917306293 1107 48-49 1.1618845469357488 1107 50-51 1.3341093115433758 1107 52-53 1.3902579815197846 1107 54-55 1.293686074139238 1107 56-57 1.2476013880580545 1107 58-59 0.9253840569164176 1107 60-61 1.2606028466319756 1107 62-63 1.1499595828480516 1107 64-65 1.066182919470318 1107 66-67 1.1861100646479166 1107 68-69 0.8945764078142702 1107 70-71 0.9627548024874528 1107 72-73 0.9868872918951688 1107 74-75 0.9258485286440354 1107 76-77 0.8670654554021446 1107 78-79 0.9265794268351577 1107 80-81 0.7057269131935016 1107 82-83 0.7975213402229251 1107 84-85 0.5609890327262619 1107 86-87 0.6522374706093501 1107 88-89 0.8160661818074431 1107 90-91 0.6156565218315002 1107 92-93 0.7383776980236014 1107 94-95 1.1971492703762614 1107 96-97 2.5113847803056633 1107 98-99 2.750603551334878 1107 100-101 2.0232912147430824 1108 1 0.5840251583904568 1108 2 0.7286280713235627 1108 3 0.7268716796483972 1108 4 0.5457996017059514 1108 5 0.6359267717171377 1108 6 0.6657900653214597 1108 7 0.5615082515936223 1108 8 0.718952492741181 1108 9 0.6410943215596419 1108 10-11 0.6441833692042067 1108 12-13 0.45043820057638584 1108 14-15 0.4144640921890925 1108 16-17 0.6403083934656735 1108 18-19 0.6768478532600994 1108 20-21 0.4834805396074273 1108 22-23 0.5252112054773548 1108 24-25 0.5946539309169339 1108 26-27 0.7733943892396198 1108 28-29 0.7368333602990091 1108 30-31 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1.683926747242534 2311 92-93 1.8289628652496823 2311 94-95 2.1344021942561433 2311 96-97 3.6395601895597824 2311 98-99 3.8056604925595483 2311 100-101 3.444863910310948 2312 1 0.04341217888269355 2312 2 -0.43985974226656666 2312 3 -0.7718493028364186 2312 4 -0.20606556893701367 2312 5 -1.7960841780082788 2312 6 -0.39124294951284355 2312 7 0.08314292112338961 2312 8 0.22774794466895543 2312 9 0.2300729015107379 2312 10-11 0.18103214097837395 2312 12-13 0.21185506731335124 2312 14-15 0.323259751788477 2312 16-17 -0.33577861966220723 2312 18-19 -0.04817152809255276 2312 20-21 0.43837482437281494 2312 22-23 0.3218947057919692 2312 24-25 0.3817486737419955 2312 26-27 0.6048564149675855 2312 28-29 0.49346906738810503 2312 30-31 0.16234306042406388 2312 32-33 -0.22256657443965366 2312 34-35 0.24673265888279872 2312 36-37 0.5859338223884762 2312 38-39 0.5733477772792384 2312 40-41 0.6742733497033129 2312 42-43 0.7244404249877263 2312 44-45 0.7736015034820483 2312 46-47 0.42064168623184983 2312 48-49 0.6906430936274042 2312 50-51 0.5053611685470578 2312 52-53 0.23312606458058482 2312 54-55 0.3933287048070966 2312 56-57 0.4272189507742752 2312 58-59 0.5930420045086429 2312 60-61 0.6902848930819978 2312 62-63 0.7450619833369458 2312 64-65 0.747429036063302 2312 66-67 0.9780535772167305 2312 68-69 0.8267690848912359 2312 70-71 0.9423840953302189 2312 72-73 0.9254311772710544 2312 74-75 0.9670863957938032 2312 76-77 0.9222088761755245 2312 78-79 1.0366052171324576 2312 80-81 0.9805652963409841 2312 82-83 1.2323473028603935 2312 84-85 1.4029710437621645 2312 86-87 1.0907277393986448 2312 88-89 1.0444773952970223 2312 90-91 1.4251256265319867 2312 92-93 1.348994948788775 2312 94-95 1.703051649514407 2312 96-97 3.186717490802307 2312 98-99 3.4779088727850915 2312 100-101 3.152941314976534 2313 1 -0.034006860236647896 2313 2 -0.3968488700688013 2313 3 -0.9047248448703868 2313 4 0.02826877851546783 2313 5 -0.23088300751350488 2313 6 0.23422659207654561 2313 7 0.08129511198570327 2313 8 0.28587737591067963 2313 9 0.658854366022652 2313 10-11 -0.4590942976718395 2313 12-13 0.246167268925106 2313 14-15 0.3966547174665749 2313 16-17 -0.2383682729927088 2313 18-19 0.10600516088031497 2313 20-21 -1.8793636622783083 2313 22-23 0.07758892398573636 2313 24-25 0.40960001294328663 2313 26-27 0.3792168397695974 2313 28-29 0.1152424100995475 2313 30-31 0.47651938444674613 2313 32-33 0.6905110836418373 2313 34-35 0.3689049585002593 2313 36-37 -0.2966666799156883 2313 38-39 0.3410920699560194 2313 40-41 0.6437134165887315 2313 42-43 0.4643892821959881 2313 44-45 0.5403238526995651 2313 46-47 0.48214889731572086 2313 48-49 0.2743039421034048 2313 50-51 0.5022740217607762 2313 52-53 0.5514005303432832 2313 54-55 0.6633200953004739 2313 56-57 0.6122671905531831 2313 58-59 0.7449532652124624 2313 60-61 0.429160382032304 2313 62-63 0.5028975467370884 2313 64-65 0.341400059018671 2313 66-67 0.46221747634449883 2313 68-69 0.2763544517159424 2313 70-71 0.5851988068039233 2313 72-73 0.43944493257527384 2313 74-75 0.6796573537216837 2313 76-77 0.5978176794375152 2313 78-79 0.3888547203025503 2313 80-81 0.6405116818411827 2313 82-83 0.7722249118394942 2313 84-85 0.4119276677738988 2313 86-87 0.6064226709041307 2313 88-89 1.054111333673525 2313 90-91 1.0485304624537015 2313 92-93 1.3598235184162952 2313 94-95 1.8065089719080838 2313 96-97 3.0093058336298757 2313 98-99 3.0642303739910854 2313 100-101 2.6445067356147973 2314 1 -0.12398745100932018 2314 2 -0.1101512706655825 2314 3 -1.0583290209624998 2314 4 -0.16007618769106813 2314 5 -0.5642159379269387 2314 6 -0.5659523043603727 2314 7 -0.22261522567269054 2314 8 -1.8724858598354501 2314 9 -1.382089260878125 2314 10-11 -0.7533238515013636 2314 12-13 -0.37874673645560364 2314 14-15 -0.4042268137211522 2314 16-17 -0.417149532382183 2314 18-19 -1.6325164569236819 2314 20-21 -0.9632770563719646 2314 22-23 -1.6896269225086868 2314 24-25 -1.4275441368323953 2314 26-27 -1.6235279081694713 2314 28-29 -2.6210182226688907 2314 30-31 -1.2814510043939862 2314 32-33 -1.241748122790149 2314 34-35 -1.5915728296766858 2314 36-37 -2.896812421800597 2314 38-39 -3.245518067048266 2314 40-41 -3.7124949459562977 2314 42-43 -2.6736292325491533 2314 44-45 -3.2760246223637246 2314 46-47 -3.4557168820062003 2314 48-49 -1.7877827915861069 2314 50-51 -1.4308297623478303 2314 52-53 -2.581683136550172 2314 54-55 -1.9585682118488208 2314 56-57 -1.9612348108075324 2314 58-59 -4.006875156527251 2314 60-61 -2.381722599667075 2314 62-63 -2.2995735898078635 2314 64-65 -1.660488023646682 2314 66-67 -2.9942170106060786 2314 68-69 -2.7673454063411356 2314 70-71 -2.164162363689911 2314 72-73 -1.3762527117247316 2314 74-75 -1.9124576476770265 2314 76-77 -0.9514108728482462 2314 78-79 -0.6513197037234484 2314 80-81 -0.5043885609468362 2314 82-83 -0.48405338608869286 2314 84-85 -0.30007626587555336 2314 86-87 0.01597161358474608 2314 88-89 0.1211963750994478 2314 90-91 0.5123117909151595 2314 92-93 0.6746931623156875 2314 94-95 1.426979058558885 2314 96-97 2.8337575747618953 2314 98-99 2.926909227258017 2314 100-101 2.4816827448428143 2315 1 -2.254448696719386 2315 2 -1.8613951960866082 2315 3 -1.499510535294359 2315 4 -1.7327669663755287 2315 5 -1.7201406583145342 2315 6 -2.8309827791561517 2315 7 -2.04701757229439 2315 8 -1.3648858886247481 2315 9 -1.1224358487952841 2315 10-11 -3.363379762965451 2315 12-13 -1.8851607096947731 2315 14-15 -1.2521666161766234 2315 16-17 -2.166850531268068 2315 18-19 -1.1093603491488864 2315 20-21 -0.8072892675884731 2315 22-23 -1.0007883944938314 2315 24-25 -3.115528166115233 2315 26-27 -4.4599148106538 2315 28-29 -5.978701220163778 2315 30-31 -8.585822479632618 2315 32-33 -7.04137826841847 2315 34-35 -6.152078280181492 2315 36-37 -3.939150593988515 2315 38-39 -6.383004140591705 2315 40-41 -4.023100553113082 2315 42-43 -3.799663572602192 2315 44-45 -2.985723366577407 2315 46-47 -2.138238472144984 2315 48-49 -2.7172275253107934 2315 50-51 -2.600710407933189 2315 52-53 -1.944865936853045 2315 54-55 -3.448551536821995 2315 56-57 -3.2297428604279474 2315 58-59 -2.217628253763845 2315 60-61 -2.492059243525077 2315 62-63 -2.952948217181053 2315 64-65 -2.144436336571683 2315 66-67 -3.072560443450996 2315 68-69 -2.0269818349403685 2315 70-71 -2.557015677066577 2315 72-73 -1.7449328361650132 2315 74-75 -2.142017979253378 2315 76-77 -1.4458060478497323 2315 78-79 -1.4483887400236704 2315 80-81 -1.185050780939683 2315 82-83 -1.0539882502979374 2315 84-85 -1.0297399671953826 2315 86-87 -1.700869374005741 2315 88-89 -1.9252307773230015 2315 90-91 -1.5609072200108223 2315 92-93 -0.902811378241605 2315 94-95 -0.16587552169385944 2315 96-97 1.0303380926314585 2315 98-99 0.924767564073731 2315 100-101 0.6968346561365202 2316 1 -3.5950160898921943 2316 2 -2.202043567541203 2316 3 -2.5233726701705024 2316 4 -3.0621064401610134 2316 5 -2.266750474485818 2316 6 -5.600928291195455 2316 7 -3.395923323195575 2316 8 -4.593289542108259 2316 9 -6.518727524548623 2316 10-11 -5.657083982174463 2316 12-13 -3.4527054161204376 2316 14-15 -4.879486924567573 2316 16-17 -4.721366553743145 2316 18-19 -2.9263574499660443 2316 20-21 -3.261138862857422 2316 22-23 -4.076659432170384 2316 24-25 -2.897183859289324 2316 26-27 -3.895832927980905 2316 28-29 -3.5829403129504556 2316 30-31 -3.5008000943491773 2316 32-33 -3.0430968520522015 2316 34-35 -5.1433707095940875 2316 36-37 -5.390305456533895 2316 38-39 -5.3670442031935295 2316 40-41 -4.664346319793005 2316 42-43 -3.9752473378196953 2316 44-45 -3.588891701984643 2316 46-47 -3.7916129413764423 2316 48-49 -4.343046528428054 2316 50-51 -5.246545879306968 2316 52-53 -3.0136674101767156 2316 54-55 -3.3338201441879747 2316 56-57 -2.769837532858773 2316 58-59 -3.121583200975625 2316 60-61 -3.0898565540840046 2316 62-63 -2.394802202916047 2316 64-65 -2.8309124824544547 2316 66-67 -3.5784013026866894 2316 68-69 -2.614592719062852 2316 70-71 -2.0762375960433985 2316 72-73 -2.512415325613784 2316 74-75 -3.4033348945741473 2316 76-77 -3.5479192602982614 2316 78-79 -3.716272112055538 2316 80-81 -3.878634575020733 2316 82-83 -2.791406550427503 2316 84-85 -2.7343989443995937 2316 86-87 -2.4451868633903686 2316 88-89 -2.3594824257521836 2316 90-91 -1.9793749184958074 2316 92-93 -1.8006989675582474 2316 94-95 -2.107564744687199 2316 96-97 -0.22736847414596006 2316 98-99 0.23247741808832956 2316 100-101 -0.05092481110656344 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 20137.0 3 6737.0 4 6329.0 5 7244.0 6 8188.0 7 9861.0 8 12087.0 9 14481.0 10 17333.0 11 20131.0 12 23045.0 13 26133.0 14 28526.0 15 31068.0 16 34305.0 17 36720.0 18 35082.0 19 35091.0 20 38332.0 21 42046.0 22 46322.0 23 51579.0 24 57850.0 25 64669.0 26 72276.0 27 81897.0 28 92458.0 29 104489.0 30 118397.0 31 133978.0 32 153267.0 33 174557.0 34 199590.0 35 219523.0 36 215624.0 37 154590.0 38 34555.0 39 264.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.783034924120926 19.033969475245783 13.90975630566963 23.273239294963666 2 15.691939021887064 20.20264761670806 40.14257792696508 23.962835434439793 3 19.40279619040081 24.629452831806457 29.551971414814957 26.415779562977765 4 12.96039077789885 16.669490802182292 37.273600187341195 33.09651823257766 5 13.96662232085398 37.28779250612108 34.14197448665977 14.603610686365167 6 31.77382377155795 36.25945276513607 17.706616842835192 14.260106620470783 7 28.50143829632491 32.56367197855729 20.964946964240287 17.969942760877515 8 26.398397990644224 34.91672074588922 19.85644749395371 18.82843376951284 9 26.65107798573681 16.827652577203967 20.22917917389612 36.292090263163104 10-11 25.128716492223823 26.52253443108187 27.51777704891402 20.83097202778029 12-13 26.117830702577542 23.853906058022307 27.833827819768352 22.1944354196318 14-15 22.97665398924477 25.249870810674096 26.047515700084535 25.7259594999966 16-17 22.575110197902738 28.50188569101052 26.514613074674028 22.408391036412716 18-19 22.406862624832772 27.13936964643454 28.858510111250872 21.595257617481813 20-21 23.227219078663936 26.330215989505234 28.72890781462903 21.713657117201805 22-23 22.78308428895913 26.261021770859372 28.614299280630146 22.341594659551347 24-25 22.652947123022013 26.502155928287017 28.78015720740236 22.064739741288605 26-27 22.493067316914964 26.7430016573244 28.64163150207893 22.122299523681708 28-29 22.310850103479428 26.916804023720942 28.78772779968545 21.984618073114184 30-31 22.41579937262682 26.563584461413907 28.690067581675287 22.330548584283978 32-33 22.25402530308407 26.70797880681021 28.674847942164376 22.36314794794134 34-35 22.906728565464824 26.71481231529776 28.360240114975337 22.018219004262075 36-37 22.726666217080442 26.435278247471643 28.515831312773223 22.3222242226747 38-39 22.365155900679152 26.76711809443702 28.61852954273988 22.249196462143946 40-41 22.550698406891076 26.638125020494673 28.562097879051425 22.249078693562826 42-43 22.400646278187534 26.631359506472474 28.382638100301815 22.585356115038177 44-45 22.486424701021672 26.762107632197257 28.27560290662372 22.475864760157343 46-47 22.586455338843454 26.858912272341406 28.007372956268718 22.547259432546422 48-49 22.504310765493326 26.924493463131892 27.972942608891575 22.598253162483207 50-51 22.311849647700793 27.125006113618582 27.94093945216612 22.6222047865145 52-53 22.57540840305607 27.148511234244655 27.61909971237114 22.656980650328137 54-55 22.24939041832968 26.922499022729234 27.90967665237189 22.9184339065692 56-57 22.322842110888345 27.231125672452755 27.773833450631503 22.672198766027396 58-59 22.448409909885747 26.81597406043131 27.839234422910202 22.896381606772742 60-61 22.44434694773771 26.7883202795694 28.052422150289182 22.71491062240371 62-63 22.206013351315487 26.90056803006119 27.966544780449095 22.926873838174235 64-65 22.070136761425914 26.86104531801182 27.874851385504712 23.19396653505755 66-67 22.117631822775845 27.02637826882519 27.633573586962417 23.222416321436548 68-69 22.124943775362958 26.837645933997173 27.801667734616824 23.23574255602304 70-71 22.135873192390157 26.898872020109437 27.705485300292782 23.25976948720762 72-73 22.259239575355853 26.72810841233792 27.709792662476563 23.302859349829667 74-75 22.20978223324718 27.08428851402016 27.424233107694036 23.281696145038623 76-77 22.274795542593264 26.93474038222618 27.441044365214136 23.349419709966426 78-79 22.291306154740763 27.05165247730245 27.40114871325874 23.255892654698048 80-81 22.254761047373673 27.160479050566106 27.316138498318658 23.268621403741562 82-83 22.3458017557566 27.10797478003517 27.15248569852014 23.393737765688087 84-85 22.348598874577007 27.016424127619743 27.11709033197977 23.517886665823482 86-87 22.415479826852117 26.930367433805923 27.242197844275008 23.411954895066952 88-89 22.40692201285499 26.860533365517224 27.037503520113386 23.6950411015144 90-91 22.38667678701811 27.003265319427545 27.15678299087745 23.453274902676892 92-93 22.641831383431 26.814896795788762 27.079677459888295 23.46359436089194 94-95 22.505415843294106 26.70770581803633 27.0527640041771 23.734114334492467 96-97 22.66147845882715 26.647167664731423 26.925220124864275 23.766133751577154 98-99 22.647242453197144 26.584615943650757 26.61232313438937 24.155818468762725 100-101 22.74831451254718 26.574754576797126 26.782251601860928 23.894679308794764 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2918.0 1 1734.5 2 531.0 3 497.0 4 423.5 5 414.5 6 610.0 7 1118.0 8 1707.5 9 1972.0 10 1821.5 11 1600.0 12 1601.0 13 1741.0 14 1913.0 15 2147.0 16 2372.0 17 2618.0 18 2892.5 19 3148.0 20 3541.5 21 4171.0 22 5127.0 23 6189.5 24 7356.5 25 9151.0 26 11857.5 27 15246.5 28 18456.5 29 22059.0 30 26307.5 31 30309.5 32 35152.5 33 40255.0 34 45291.0 35 49922.0 36 54099.0 37 59030.5 38 63290.5 39 66477.0 40 69826.5 41 74584.0 42 79776.0 43 84068.0 44 87679.5 45 90713.0 46 92773.5 47 95398.0 48 99832.0 49 100739.5 50 98237.5 51 95382.0 52 91891.5 53 89116.0 54 84945.5 55 77795.0 56 69685.5 57 61662.0 58 54185.5 59 46683.0 60 38933.5 61 31862.0 62 25452.0 63 19629.5 64 14729.0 65 11238.5 66 8771.0 67 6871.5 68 5159.0 69 3713.5 70 2788.0 71 2235.0 72 1857.5 73 1407.0 74 1032.5 75 792.5 76 556.5 77 370.0 78 244.0 79 176.5 80 110.0 81 77.5 82 56.0 83 29.0 84 19.0 85 18.5 86 13.5 87 6.5 88 6.5 89 3.5 90 2.5 91 1.5 92 3.0 93 4.0 94 2.0 95 1.5 96 1.0 97 0.5 98 0.0 99 0.5 100 0.5 >>END_MODULE >>Per base N content warn #Base N-Count 1 0.1124029906606702 2 0.10618582890617892 3 0.11894953846837955 4 0.13340135155332286 5 0.12878994680826972 6 0.12961341194131493 7 0.1367775585988082 8 0.1361599597490243 9 0.14398287851295374 10-11 0.13885680805974734 12-13 0.13788923652841922 14-15 0.12588723221428538 16-17 0.12866642703831296 18-19 0.1468444198502858 20-21 0.1776420158261764 22-23 0.19330843998236139 24-25 0.2145538404149276 26-27 0.21725068872565065 28-29 0.2195563910981772 30-31 0.21916524515998076 32-33 0.21990636377972142 34-35 0.1889440747772218 36-37 0.1774361495429151 38-39 0.19094097772485644 40-41 0.17912425306565777 42-43 0.18190344788968532 44-45 0.18548552121843195 46-47 0.20745145364241274 48-49 0.22511478074623234 50-51 0.24257224156679064 52-53 0.2622118849899187 54-55 0.25519184473070833 56-57 0.22437366212649168 58-59 0.21747714163723808 60-61 0.19547003595660503 62-63 0.18550610784675808 64-65 0.17636564486995635 66-67 0.17171306686825094 68-69 0.17846548095922157 70-71 0.18227400719955567 72-73 0.21710658232736774 74-75 0.2294173860663935 76-77 0.2473277527101267 78-79 0.23476990943118733 80-81 0.24059592524748213 82-83 0.23715795831701844 84-85 0.2225620388337922 86-87 0.21278339037888042 88-89 0.21362744214025176 90-91 0.21663308987586674 92-93 0.22375606327670775 94-95 0.22836746802176092 96-97 8.710346551183916 98-99 0.2885010093623868 100-101 0.29976189505677997 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2428761.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.89808389916136 #Duplication Level Percentage of deduplicated Percentage of total 1 77.35469990071132 45.560436047465444 2 12.532639675365404 14.7629692615526 3 4.393103177886132 7.762360786464294 4 2.0422658789926382 4.811421883412118 5 1.0794619576561817 3.178912047399338 6 0.6546113079394142 2.313321104181322 7 0.42053401357841946 1.7338053329934968 8 0.2745148557049084 1.2934719202299096 9 0.20125488506122038 1.066817439490665 >10 0.9484092642858685 9.85687292349427 >50 0.062073051891254935 2.51590601739881 >100 0.03479816886441643 3.7658815761532973 >500 0.001136599695882994 0.4715335897957065 >1k 3.551874049626298E-4 0.41032208652340546 >5k 1.420749619850519E-4 0.49596798344533993 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6733 0.27721953703966756 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5121 0.2108482473162242 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3422 0.1408948842640342 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 8.234651330452028E-5 0.0 0.0 0.0 4 0.0 8.234651330452028E-5 0.0 0.0 0.0 5 0.0 3.293860532180811E-4 0.0 0.0 0.0 6 0.0 3.293860532180811E-4 0.0 0.0 0.0 7 1.2351976995678043E-4 3.293860532180811E-4 0.0 0.0 0.0 8 1.2351976995678043E-4 3.293860532180811E-4 0.0 0.0 0.0 9 1.2351976995678043E-4 3.293860532180811E-4 0.0 0.0 0.0 10-11 1.2351976995678043E-4 3.293860532180811E-4 0.0 0.0 0.0 12-13 1.6469302660904056E-4 3.499726815442112E-4 0.0 2.058662832613007E-5 0.0 14-15 1.6469302660904056E-4 3.705593098703413E-4 0.0 4.117325665226014E-5 0.0 16-17 2.264529115874308E-4 3.705593098703413E-4 0.0 8.234651330452028E-5 0.0 18-19 2.88212796565821E-4 3.705593098703413E-4 0.0 1.2351976995678043E-4 0.0 20-21 3.293860532180811E-4 3.705593098703413E-4 0.0 2.676261682396909E-4 0.0 22-23 3.705593098703413E-4 3.705593098703413E-4 0.0 4.7349245150099165E-4 0.0 24-25 4.529058231748616E-4 3.911459381964714E-4 0.0 0.0013381308411984545 0.0 26-27 4.529058231748616E-4 4.1173256652260145E-4 0.0 0.0025321552841139987 0.0 28-29 5.970122214577721E-4 4.1173256652260145E-4 0.0 0.007431772825732956 0.0 30-31 6.587721064361623E-4 4.1173256652260145E-4 0.0 0.02610384471753293 0.0 32-33 6.587721064361623E-4 4.1173256652260145E-4 0.0 0.06513609202387555 0.0 34-35 7.617052480668126E-4 4.1173256652260145E-4 0.0 0.12393150252330304 0.0 36-37 9.469849030019833E-4 4.323191948487315E-4 0.0 0.22381782316168614 0.0 38-39 9.469849030019833E-4 4.529058231748616E-4 0.0 0.3666684371167027 0.0 40-41 9.469849030019833E-4 4.529058231748616E-4 0.0 0.5270176851489299 0.0 42-43 9.675715313281134E-4 4.529058231748616E-4 0.0 0.686564054676438 0.0 44-45 0.0010293314163065037 4.529058231748616E-4 0.0 0.8845250726605047 0.0 46-47 0.0012146110712416742 4.7349245150099165E-4 0.0 1.1124190482307645 0.0 48-49 0.0012763709562200645 4.940790798271217E-4 0.0 1.354497210717728 0.0 50-51 0.0012763709562200645 4.940790798271217E-4 2.058662832613007E-5 1.6048100245351438 0.0 52-53 0.0012763709562200645 4.940790798271217E-4 4.117325665226014E-5 1.848267491119958 0.0 54-55 0.0012763709562200645 6.175988497839021E-4 4.117325665226014E-5 2.1167788843776725 0.0 56-57 0.0012763709562200645 6.999453630884225E-4 4.117325665226014E-5 2.4284192639786295 0.0 58-59 0.0012763709562200645 8.234651330452029E-4 4.117325665226014E-5 2.75514964214264 0.0 60-61 0.0012763709562200645 8.234651330452029E-4 4.117325665226014E-5 3.102384302119476 0.0 62-63 0.0012763709562200645 8.234651330452029E-4 4.117325665226014E-5 3.437987517092048 0.0 64-65 0.0012763709562200645 8.852250180235932E-4 4.117325665226014E-5 3.7774198449332808 0.0 66-67 0.0013381308411984545 9.058116463497232E-4 4.117325665226014E-5 4.158910654444798 0.0 68-69 0.0013587174695245847 9.469849030019833E-4 4.117325665226014E-5 4.5824599456265975 0.0 70-71 0.0013998907261768448 9.675715313281134E-4 4.117325665226014E-5 5.036992112439223 0.0 72-73 0.0014822372394813653 9.881581596542434E-4 4.117325665226014E-5 5.481230965088784 0.0 74-75 0.0015234104961336253 0.0010293314163065037 4.117325665226014E-5 5.922628863029339 0.0 76-77 0.0015234104961336253 0.0010293314163065037 4.117325665226014E-5 6.389183620784424 0.0 78-79 0.0015234104961336253 0.0010293314163065037 4.117325665226014E-5 6.892629616499935 0.0 80-81 0.001749863407721056 0.001091091301284894 4.117325665226014E-5 7.43420204787544 0.0 82-83 0.0017704500360471863 0.001111677929611024 4.117325665226014E-5 7.964369487158267 0.0 84-85 0.0018322099210255766 0.001111677929611024 4.117325665226014E-5 8.488299178058277 0.0 86-87 0.0018939698060039666 0.0012351976995678042 4.117325665226014E-5 8.957159638185889 0.0 88-89 0.0018939698060039666 0.0012351976995678042 4.117325665226014E-5 9.422211572073168 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 10000 0.0 32.7418 1 GGTATCA 6375 0.0 32.24739 1 TATCAAC 12030 0.0 27.139141 2 ATCAACG 12150 0.0 26.79532 3 TCAACGC 12215 0.0 26.729765 4 CAACGCA 12295 0.0 26.17373 5 AACGCAG 12535 0.0 25.973398 6 GTACATG 9235 0.0 25.418911 1 TACATGG 9280 0.0 24.789236 2 ACATGGG 9855 0.0 23.057894 3 ACGCAGA 14215 0.0 22.738243 7 CGCAGAG 14495 0.0 22.16963 8 CAACGCG 180 5.619462E-6 20.910074 5 GAGTACT 6890 0.0 20.382559 12-13 GCAGAGT 14900 0.0 19.956612 9 GTACCGT 170 8.5641484E-5 19.372967 6 AGAGTAC 11665 0.0 19.359718 10-11 CATGGGA 5480 0.0 19.230764 4 AGTACTT 7095 0.0 19.097376 12-13 GTACTTT 7495 0.0 18.7733 14-15 >>END_MODULE