FastQCFastQC Report
Fri 27 May 2016
SRR939760_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939760_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2428761
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74620.3072348411391652No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61740.25420368657105413No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42600.1753980733386282No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA65000.037.239551
GTATCAA99500.031.1962411
ATCAACG121050.025.4709853
TATCAAC121900.025.3774722
TCAACGC121550.025.3271834
CAACGCA123200.024.9879825
GTACATG93450.024.8865321
AACGCAG126600.024.3168986
TACATGG94450.024.0121172
ACATGGG97750.022.1766833
ACGCAGA141850.021.66927
CGCAGAG143850.021.3019718
GAGTACT73000.021.11823812-13
AGAGTAC121600.019.79701210-11
GCAGAGT153950.019.503899
GTACTTT78850.019.3709714-15
AGTACTT76800.019.11598412-13
GTGTAGC15050.018.2910691
CATGGGG45350.018.095314
CATGGGA52400.017.8333174
Produced by FastQC (version 0.11.5)