##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939760_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2428761 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.376346211092816 31.0 31.0 34.0 30.0 34.0 2 31.61200463940256 31.0 31.0 34.0 30.0 34.0 3 31.655833159376325 31.0 31.0 34.0 30.0 34.0 4 35.46676021230578 37.0 35.0 37.0 33.0 37.0 5 35.20148503702093 37.0 35.0 37.0 32.0 37.0 6 35.22481709810064 37.0 35.0 37.0 32.0 37.0 7 35.13090048794427 37.0 35.0 37.0 32.0 37.0 8 35.0910690677263 37.0 35.0 37.0 32.0 37.0 9 36.675246349887864 39.0 35.0 39.0 32.0 39.0 10-11 36.53952756158387 38.0 35.0 39.0 32.0 39.0 12-13 36.45475841385793 38.0 35.0 39.0 32.0 39.0 14-15 37.48420120382367 39.0 36.0 41.0 32.0 41.0 16-17 37.401647177305634 39.0 36.0 41.0 32.0 41.0 18-19 37.37301694155991 39.0 36.0 41.0 32.0 41.0 20-21 37.29605568435923 39.0 36.0 40.0 31.0 41.0 22-23 37.1604295358827 39.0 36.0 40.0 31.0 41.0 24-25 37.00086916744793 39.0 36.0 40.0 31.0 41.0 26-27 36.70188627040701 39.0 35.5 40.0 30.0 41.0 28-29 36.46713735933672 38.0 35.0 40.0 30.0 41.0 30-31 36.20713997795584 38.0 35.0 40.0 30.0 41.0 32-33 36.21293264343424 38.0 35.0 40.0 30.0 41.0 34-35 36.0600009634542 38.0 35.0 40.0 30.0 41.0 36-37 36.112830574931 38.0 35.0 40.0 30.0 41.0 38-39 36.01751530924615 38.0 35.0 40.0 29.0 41.0 40-41 35.94042559148471 38.0 35.0 40.0 29.0 41.0 42-43 35.72534946830915 38.0 34.5 40.0 28.5 41.0 44-45 35.482711761264284 38.0 34.0 40.0 27.0 41.0 46-47 35.2052711238364 38.0 34.0 40.0 26.5 41.0 48-49 34.90897684045487 38.0 33.5 40.0 26.0 41.0 50-51 34.685728237566394 38.0 33.0 40.0 25.5 41.0 52-53 34.441620439392764 38.0 33.0 40.0 24.0 41.0 54-55 34.17469359891731 37.5 33.0 40.0 24.0 41.0 56-57 33.80508683233961 37.0 32.5 40.0 23.5 41.0 58-59 33.42935822009659 37.0 32.0 39.5 23.0 41.0 60-61 33.112577977001436 36.0 31.0 39.0 22.0 40.5 62-63 32.81753124329648 36.0 31.0 39.0 22.0 40.0 64-65 32.36682736588738 35.5 31.0 39.0 20.5 40.0 66-67 31.409390837550504 35.0 29.5 38.0 14.5 40.0 68-69 29.399979248678648 34.0 26.0 37.0 7.5 39.5 70-71 28.733804190696407 33.0 25.0 36.5 7.5 39.0 72-73 29.337915710932446 33.5 26.5 36.0 8.5 39.0 74-75 29.46900477239218 34.0 27.5 36.0 8.0 39.0 76-77 29.049346559830298 32.5 27.5 35.0 8.0 37.0 78-79 29.595691177518084 34.0 29.0 35.0 7.5 37.0 80-81 29.410781464293933 34.0 29.0 35.0 7.0 37.0 82-83 28.956399785734373 33.5 28.5 35.0 4.0 36.5 84-85 28.58642060705026 33.0 28.0 35.0 2.0 36.0 86-87 28.187019636761296 33.0 27.0 35.0 2.0 36.0 88-89 27.728660004010276 33.0 26.5 35.0 2.0 35.0 90-91 27.220474760587805 32.5 26.0 35.0 2.0 35.0 92-93 26.703099852146835 32.0 25.0 34.0 2.0 35.0 94-95 25.84913933482957 32.0 23.5 34.0 2.0 35.0 96-97 25.31012314509332 31.5 21.5 34.0 2.0 35.0 98-99 24.619752622839382 31.0 18.0 34.0 2.0 35.0 100-101 23.113889139359532 30.0 2.0 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.3177259685594649 1101 2 0.32992057075226455 1101 3 0.3680320255648013 1101 4 0.24862702805938142 1101 5 0.34787452075304515 1101 6 0.28017537826020344 1101 7 0.2583926675783772 1101 8 0.2557869707575122 1101 9 0.38348520653576657 1101 10-11 0.43296150138325373 1101 12-13 0.48451798392466117 1101 14-15 0.48435626782308105 1101 16-17 0.48170510787845444 1101 18-19 0.3283078843596172 1101 20-21 0.41826549057856965 1101 22-23 0.34039434061052987 1101 24-25 0.3550793521986222 1101 26-27 0.4185441134487675 1101 28-29 0.48207291774508576 1101 30-31 0.20065389243224985 1101 32-33 0.2396259789962869 1101 34-35 0.39804351309754793 1101 36-37 0.6658488154655018 1101 38-39 0.6747122494313942 1101 40-41 0.506363148758787 1101 42-43 0.3895158512946253 1101 44-45 0.3529127200491047 1101 46-47 0.3631833989025779 1101 48-49 0.40069433946794675 1101 50-51 0.3993040939916668 1101 52-53 0.23831984782939486 1101 54-55 -0.06819817410192286 1101 56-57 0.11695865907795167 1101 58-59 0.17324837677097804 1101 60-61 -2.31093549984962E-6 1101 62-63 0.12559164979841597 1101 64-65 0.05234933891911453 1101 66-67 -0.3151165370947666 1101 68-69 -8.73382447329426 1101 70-71 -6.415520122754845 1101 72-73 -1.3855866164445452 1101 74-75 -0.40230573989855145 1101 76-77 -0.17319791229087755 1101 78-79 -0.31349991213682316 1101 80-81 -0.3624207770420753 1101 82-83 -0.19041405097341624 1101 84-85 -0.1186898752927732 1101 86-87 -0.2581595646771291 1101 88-89 -0.05521103464957022 1101 90-91 -0.4035218203921467 1101 92-93 -0.5611975035114121 1101 94-95 -0.19363276898071646 1101 96-97 -0.8861144784327273 1101 98-99 -0.6259225310094045 1101 100-101 -0.6435135980225688 1102 1 0.24420283534388432 1102 2 0.12744404552091382 1102 3 0.2771631284110647 1102 4 0.11599928595672537 1102 5 0.28449548429550475 1102 6 0.3480771908953315 1102 7 0.36809963244427735 1102 8 0.24070259490814294 1102 9 0.42931319327065154 1102 10-11 0.4136003383566944 1102 12-13 0.2535292246486378 1102 14-15 0.5005193526313221 1102 16-17 0.5272849672193516 1102 18-19 0.31076627306382676 1102 20-21 0.33144368763552023 1102 22-23 0.4897840652016541 1102 24-25 0.4001823497692598 1102 26-27 0.406489546363602 1102 28-29 0.4170245332719489 1102 30-31 0.3195125919556503 1102 32-33 0.30634776705906575 1102 34-35 0.17671210795616332 1102 36-37 0.2566716265505278 1102 38-39 0.48495761756027633 1102 40-41 0.3543237332982585 1102 42-43 0.2864420358651216 1102 44-45 0.26641285594769926 1102 46-47 0.3338189781439951 1102 48-49 0.31682079138189323 1102 50-51 0.2647483319869153 1102 52-53 0.3624608956805915 1102 54-55 0.21897554943564046 1102 56-57 0.18019847873910066 1102 58-59 -0.03635460317634198 1102 60-61 0.3327517881069326 1102 62-63 0.31928299052843556 1102 64-65 0.3390353612430559 1102 66-67 0.6454503053281897 1102 68-69 -4.708431196905117 1102 70-71 0.36410603000521036 1102 72-73 0.9226614920725886 1102 74-75 0.5536977706063411 1102 76-77 0.3269216902746628 1102 78-79 0.11016334484897428 1102 80-81 0.1539374914844167 1102 82-83 0.16985475837345732 1102 84-85 0.13985459245442655 1102 86-87 3.2229975431974367E-4 1102 88-89 0.06034362556632544 1102 90-91 0.018296445352955715 1102 92-93 -0.24603788570281182 1102 94-95 -0.07733144071385212 1102 96-97 -0.5714454376084355 1102 98-99 -0.2718286649692949 1102 100-101 -0.2536441122944275 1103 1 0.13611854729695239 1103 2 0.11786064849077249 1103 3 0.089816526940389 1103 4 0.13179092465218645 1103 5 0.1883918087866121 1103 6 0.08593170517117699 1103 7 0.20117101496409617 1103 8 0.22046598988521993 1103 9 0.2074443252423137 1103 10-11 0.25311769010560425 1103 12-13 0.2485487620288822 1103 14-15 0.31142070123121357 1103 16-17 0.25374089161326907 1103 18-19 0.24399436819325615 1103 20-21 0.3075939042785123 1103 22-23 0.20528716658999002 1103 24-25 0.3417192411139496 1103 26-27 0.12967326420409364 1103 28-29 0.14091087305767047 1103 30-31 0.11185198922507311 1103 32-33 0.21063528226321893 1103 34-35 0.09776661691470423 1103 36-37 0.029246624464221327 1103 38-39 0.16377889059544515 1103 40-41 0.10199641811316695 1103 42-43 0.012971663927466182 1103 44-45 0.14974172127308094 1103 46-47 -0.010325383562296508 1103 48-49 0.021746530135622777 1103 50-51 -0.07113043655623841 1103 52-53 -0.04909965025827745 1103 54-55 -0.11008430045406215 1103 56-57 -0.022693393252836813 1103 58-59 -0.009278918075636966 1103 60-61 -0.04438079947525608 1103 62-63 -0.1489117348589346 1103 64-65 -0.24144084716846947 1103 66-67 -0.03251443950424715 1103 68-69 -4.3434970834059 1103 70-71 0.20223793003113144 1103 72-73 0.6149576124385092 1103 74-75 0.28375003522171127 1103 76-77 0.14201410418796456 1103 78-79 -0.16357119344332816 1103 80-81 0.010075758975560234 1103 82-83 -0.0894416153026647 1103 84-85 -0.03014151557831468 1103 86-87 -0.04596367786428246 1103 88-89 -0.22472658123966838 1103 90-91 -0.40055825281279667 1103 92-93 -0.31938655058079846 1103 94-95 -0.19917113745058046 1103 96-97 -0.4486919778563241 1103 98-99 -0.2722163108165212 1103 100-101 -0.3678942513196368 1104 1 0.11185831246208267 1104 2 0.1593337169614628 1104 3 0.11434745334142704 1104 4 0.1572026658068708 1104 5 0.21984693418936985 1104 6 0.13953216795218992 1104 7 0.07077937328377715 1104 8 0.10247346444624839 1104 9 0.1394721863079127 1104 10-11 0.24786783398869972 1104 12-13 0.11194946650405058 1104 14-15 0.2892730087332964 1104 16-17 0.28353624643412445 1104 18-19 0.1327939342624589 1104 20-21 0.09499033421149505 1104 22-23 0.05658601508367411 1104 24-25 0.09254309141076789 1104 26-27 0.16537157926317292 1104 28-29 0.13046812528067875 1104 30-31 -0.018179392744428924 1104 32-33 0.20681336463647426 1104 34-35 0.22464324249925482 1104 36-37 0.22148966986945595 1104 38-39 0.2341153488781771 1104 40-41 0.15669753134879016 1104 42-43 0.1287262369074682 1104 44-45 0.19953667866749214 1104 46-47 0.18569586256095505 1104 48-49 0.3571994711648969 1104 50-51 0.2654017380582161 1104 52-53 0.23199043159684862 1104 54-55 0.2916371448240014 1104 56-57 0.2590083573370521 1104 58-59 0.07331601164612067 1104 60-61 0.11970561280904946 1104 62-63 0.3297923163884704 1104 64-65 0.3702757259557927 1104 66-67 0.5591485104892051 1104 68-69 -4.134616295147346 1104 70-71 0.6490271659567135 1104 72-73 0.8890186924669479 1104 74-75 0.4565578971999251 1104 76-77 0.36537843678384974 1104 78-79 -0.025134315802098683 1104 80-81 0.018744071282089436 1104 82-83 0.08370302878723734 1104 84-85 0.05645557202183227 1104 86-87 -0.03903410624582904 1104 88-89 -0.11570912244322429 1104 90-91 -0.05579633333139711 1104 92-93 -0.27199668554387557 1104 94-95 0.050071323590589856 1104 96-97 -0.39725049742810725 1104 98-99 -0.08863120047180573 1104 100-101 0.06361857451484809 1105 1 0.15381422956665958 1105 2 0.21872515344557186 1105 3 0.16749072393964326 1105 4 0.10087026433221524 1105 5 0.1618173459932848 1105 6 0.23316109414446373 1105 7 0.033350376518946234 1105 8 0.17338373156454878 1105 9 0.2739105226490963 1105 10-11 0.22949565406908334 1105 12-13 0.20757557217040556 1105 14-15 0.23034864017349577 1105 16-17 0.31944686403900135 1105 18-19 0.22362995762981797 1105 20-21 0.2555317528148535 1105 22-23 0.21473491722900206 1105 24-25 0.3159972970715401 1105 26-27 0.35325095128626316 1105 28-29 0.3476912438508961 1105 30-31 0.3346055498767626 1105 32-33 0.37160175984752186 1105 34-35 0.2471977966268568 1105 36-37 0.1407192944211033 1105 38-39 0.33615803220344986 1105 40-41 0.24207903302340128 1105 42-43 0.2884205472627386 1105 44-45 0.23980268610256417 1105 46-47 0.32164054864972513 1105 48-49 0.254555317221687 1105 50-51 0.2939390684434997 1105 52-53 0.14728688384096955 1105 54-55 0.167051491249687 1105 56-57 0.20009230540800615 1105 58-59 0.18308751815626323 1105 60-61 0.2322217559834101 1105 62-63 0.17428141982002643 1105 64-65 0.1042146783216964 1105 66-67 0.6661738952224852 1105 68-69 1.9106437005517662 1105 70-71 2.2237409550493368 1105 72-73 1.3704039936537278 1105 74-75 0.8864285023060958 1105 76-77 0.5901992430930854 1105 78-79 0.6035642087087929 1105 80-81 0.6056458827630777 1105 82-83 0.4613777166462647 1105 84-85 0.32416984704330787 1105 86-87 0.4203498452705894 1105 88-89 0.6606125683990989 1105 90-91 0.4911265429872351 1105 92-93 0.3849032368738392 1105 94-95 0.5412966840868307 1105 96-97 0.27966191181666034 1105 98-99 0.5595776942836075 1105 100-101 0.6370821957897412 1106 1 0.3727636812690456 1106 2 0.38803704590028687 1106 3 0.39014936462785244 1106 4 0.2709213451633872 1106 5 0.42003518666242456 1106 6 0.4375811227052182 1106 7 0.35410845526502754 1106 8 0.2835612162575174 1106 9 0.40389110239533466 1106 10-11 0.4496919893903666 1106 12-13 0.42495954741422537 1106 14-15 0.5696185525571522 1106 16-17 0.5749384508035646 1106 18-19 0.4006367613421844 1106 20-21 0.5797450073367472 1106 22-23 0.4824426516828666 1106 24-25 0.5020084882245968 1106 26-27 0.4798107977258397 1106 28-29 0.48769557565373844 1106 30-31 0.6435121074579442 1106 32-33 0.7419742154101101 1106 34-35 0.8525947328367494 1106 36-37 0.8887348783136844 1106 38-39 0.9387253871013002 1106 40-41 0.7941348510187964 1106 42-43 0.6576511025018377 1106 44-45 0.8227898284638826 1106 46-47 0.8207453756293077 1106 48-49 0.7824808737987397 1106 50-51 0.8358267409539124 1106 52-53 0.9044449562284385 1106 54-55 0.8686290225724633 1106 56-57 0.8486807602923392 1106 58-59 0.9643215388376376 1106 60-61 0.9585574614314112 1106 62-63 0.9860678977066399 1106 64-65 0.8661516433042991 1106 66-67 1.4813552745638958 1106 68-69 2.9154295478762435 1106 70-71 3.2969073572155025 1106 72-73 2.1082358667146863 1106 74-75 1.5386012922255823 1106 76-77 1.0587304175018026 1106 78-79 0.9008656412505651 1106 80-81 0.9408433043468492 1106 82-83 1.112065837438653 1106 84-85 1.1540455759651813 1106 86-87 1.1542903816723076 1106 88-89 1.372855011588893 1106 90-91 1.1932419067129594 1106 92-93 1.1876690996179526 1106 94-95 1.5018113737999244 1106 96-97 1.3313534344652318 1106 98-99 1.5300376469649528 1106 100-101 1.438554478875048 1107 1 0.13753095847474128 1107 2 0.10323781305270785 1107 3 0.20707242694406958 1107 4 0.11213191602461592 1107 5 0.24460874974361957 1107 6 0.15961179795126412 1107 7 0.15243661637789074 1107 8 0.22640388490626862 1107 9 0.18077127687639205 1107 10-11 0.1870480804937884 1107 12-13 0.17424814104045794 1107 14-15 0.18964108155594062 1107 16-17 0.1881799003635578 1107 18-19 0.24664576480358846 1107 20-21 0.3217516887243548 1107 22-23 0.2168900469454016 1107 24-25 0.2593144757027588 1107 26-27 0.25503622334894516 1107 28-29 0.34120418577320777 1107 30-31 0.04536312038126766 1107 32-33 0.11242806419319606 1107 34-35 0.19650952954678758 1107 36-37 0.1209151320056705 1107 38-39 0.261160322918748 1107 40-41 0.24174780797362416 1107 42-43 0.13075804268131463 1107 44-45 -0.04969481763687611 1107 46-47 0.0036381885938610026 1107 48-49 0.09300602598138141 1107 50-51 0.03478858949826957 1107 52-53 0.04049311755832008 1107 54-55 0.0940807554471732 1107 56-57 0.006235570457384654 1107 58-59 0.12485320150758383 1107 60-61 0.06582079165417554 1107 62-63 0.16268792396559917 1107 64-65 0.12385198998774882 1107 66-67 0.6919159631564327 1107 68-69 2.045859362110342 1107 70-71 2.2100418584579202 1107 72-73 1.2971729211532086 1107 74-75 0.717059513885971 1107 76-77 0.46656531425632153 1107 78-79 0.5806737717641361 1107 80-81 0.5148861948947072 1107 82-83 0.6562262694094834 1107 84-85 0.6417719510343538 1107 86-87 0.5771712858704845 1107 88-89 0.6455077774609173 1107 90-91 0.6736646679191303 1107 92-93 0.4506904133257841 1107 94-95 0.9889538395384854 1107 96-97 0.47696183950435156 1107 98-99 0.6364664819132528 1107 100-101 0.7683794959650712 1108 1 -0.06044038670864893 1108 2 -0.0665590204829698 1108 3 -0.009340917930099835 1108 4 0.035901879095632694 1108 5 0.1350057553780175 1108 6 0.024966412554057626 1108 7 0.02379694394841181 1108 8 -0.007303290013453534 1108 9 -0.16735477339467764 1108 10-11 -0.05597038906957863 1108 12-13 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1.8114340082556772 2316 72-73 0.8170018299820718 2316 74-75 0.2868021216315668 2316 76-77 -0.11932024527689578 2316 78-79 -0.29599704045400443 2316 80-81 -0.3457346498679499 2316 82-83 -0.3009805974834414 2316 84-85 -0.41594371450100454 2316 86-87 -0.22909149947518515 2316 88-89 -0.23026660768018203 2316 90-91 -0.28256876924670493 2316 92-93 -0.2152502975394377 2316 94-95 0.13136370285151955 2316 96-97 0.1895966667859561 2316 98-99 0.016199965571257025 2316 100-101 -0.2328147715508493 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5.0 3 22.0 4 40.0 5 78.0 6 244.0 7 624.0 8 1463.0 9 2506.0 10 3880.0 11 5865.0 12 8169.0 13 10671.0 14 13225.0 15 16303.0 16 19544.0 17 23106.0 18 21781.0 19 18807.0 20 19080.0 21 19986.0 22 21852.0 23 24782.0 24 29635.0 25 36207.0 26 44441.0 27 55181.0 28 67894.0 29 81594.0 30 96979.0 31 115575.0 32 138194.0 33 167372.0 34 207535.0 35 259913.0 36 326915.0 37 356657.0 38 198492.0 39 14144.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 41.71514584937752 19.946832826096806 14.877501348741243 23.460519975784432 2 15.802277253655813 21.10680886323464 39.048953375932996 24.041960507176544 3 19.167105848251783 24.93980467399002 30.172145456940992 25.720944020817203 4 13.363192179057554 17.443997165633014 36.917671191195836 32.27513946411359 5 13.664662764265401 37.655495950404344 34.342736893420145 14.337104391910113 6 31.678127242655822 36.844959219947945 17.304460998838504 14.172452538557726 7 28.251853517081344 32.67843151302248 20.89756876036794 18.172146209528233 8 26.18907335880311 35.48451247364397 19.652283612920332 18.67413055463259 9 26.0804994810111 16.709136880903472 20.18671248426667 37.02365115381876 10-11 24.969418563621534 26.734927808870452 27.508820340906333 20.786833286601684 12-13 25.786028349434137 23.93380822567556 27.965596450206505 22.3145669746838 14-15 22.493073628899673 25.434182284712247 26.113952752041058 25.95879133434702 16-17 22.210137834078264 28.732763534874987 26.678726350780344 22.3783722802664 18-19 22.12325543764907 27.280555806026204 28.95309583775431 21.643092918570414 20-21 22.874111532588014 26.425963690951885 28.854568234585454 21.845356541874644 22-23 22.530294252913315 26.397368864206893 28.671100202942984 22.401236679936805 24-25 22.481929561160747 26.61774457289822 28.769933787186417 22.130392078754607 26-27 22.222393777882083 26.917659459270283 28.682102558718352 22.177844204129276 28-29 22.118346520803335 27.06007083869015 28.777636301689235 22.043946338817275 30-31 22.242324028676503 26.68786551754237 28.714362296953 22.35544815682813 32-33 22.183836464456917 26.77065887925985 28.631422268840524 22.414082387442704 34-35 22.68500847587084 26.778316758342775 28.3906056799246 22.146069085861786 36-37 22.573302535922757 26.497932138532292 28.584753468212142 22.34401185733281 38-39 22.23813759994593 26.803220195398524 28.739318683080455 22.21932352157509 40-41 22.326908511986645 26.67833129820548 28.688543003024353 22.30621718678352 42-43 22.28387335840368 26.64843855782918 28.433417906427437 22.6342701773397 44-45 22.370425140765295 26.7924636394529 28.293479284529198 22.543631935252602 46-47 22.441583115848776 26.877588049047617 28.025061639227378 22.655767195876226 48-49 22.383077810748432 26.921838938344873 28.02876689746126 22.666316353445435 50-51 22.208853175514932 27.12428920414562 27.981726145644764 22.68513147469469 52-53 22.504475846908523 27.12383828852679 27.67886390747101 22.692821957093674 54-55 22.13984558741281 26.897800076919953 27.953729371536774 23.00862496413046 56-57 22.258112878665877 27.124872163133638 27.83219153679787 22.78482342140262 58-59 22.319977186695397 26.841067654419614 27.853850723159105 22.98510443572588 60-61 22.322735763652762 26.84005973164408 28.09798196726948 22.739222537433676 62-63 22.080183175128894 26.94065666027371 27.951088518450508 23.02807164614689 64-65 21.970934576341463 26.83924583648069 27.874296746985795 23.315522840192052 66-67 21.793486464572144 27.057225012517144 27.875355614816673 23.273932908094036 68-69 21.67609373148612 26.737758584288052 28.26398979796724 23.322157886258584 70-71 21.685354908451767 26.86978376023867 28.07931154886414 23.36554978244542 72-73 21.81765713227234 26.774667579222605 27.954162628854178 23.453512659650876 74-75 22.024035798348056 27.135061335354166 27.282031428577312 23.558871437720473 76-77 21.87381327973442 27.07868084072309 27.404104765003645 23.643401114538843 78-79 21.964065278062574 27.177939295717003 27.290122069021745 23.56787335719868 80-81 21.869204131306013 27.2706423765406 27.208613907676195 23.651539584477195 82-83 21.96102126187849 27.277252589798913 27.016893394159986 23.74483275416262 84-85 21.942773431348105 27.109465179509044 27.03949089850559 23.90827049063726 86-87 21.91316742917033 27.096222863663606 27.148184144686226 23.84242556247984 88-89 21.784622529778122 27.04534361130365 26.994803303503918 24.17523055541431 90-91 21.7446405359814 27.13206142606396 27.076271203887114 24.047026834067527 92-93 21.86295520845557 27.01928401878375 27.069043251622787 24.048717521137895 94-95 21.590223065070912 26.830651286051665 27.26107279418764 24.318052854689782 96-97 21.703612512438994 26.73282677569536 27.221656337643047 24.341904374222594 98-99 21.662980269711653 26.812926919565438 26.877848961638545 24.64624384908436 100-101 21.415140236906044 26.784819779820225 27.11833628403854 24.681703699235193 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 629.0 1 421.5 2 275.0 3 352.0 4 321.5 5 337.5 6 576.5 7 1197.5 8 1880.0 9 2159.0 10 2036.0 11 1839.5 12 1897.0 13 1996.0 14 2150.5 15 2313.0 16 2512.0 17 2727.5 18 2866.0 19 3163.5 20 3585.5 21 4214.0 22 5124.0 23 6154.0 24 7284.0 25 8962.5 26 11784.0 27 15302.0 28 18485.5 29 22213.5 30 26569.5 31 30458.0 32 35260.0 33 40415.5 34 45230.0 35 49893.5 36 54525.5 37 59350.0 38 63378.0 39 65909.0 40 69046.5 41 74426.5 42 79728.0 43 84048.5 44 87971.5 45 91578.5 46 93297.0 47 95409.0 48 100898.0 49 102403.5 50 98945.5 51 95469.0 52 91835.5 53 89216.0 54 85911.0 55 78904.5 56 69975.0 57 61982.0 58 54663.5 59 46797.5 60 38950.0 61 31400.0 62 24738.5 63 19012.0 64 14047.5 65 10474.5 66 8080.0 67 6554.0 68 4791.5 69 3180.5 70 2317.5 71 1844.0 72 1558.0 73 1228.5 74 901.5 75 706.5 76 441.5 77 230.5 78 147.5 79 85.5 80 54.5 81 34.0 82 15.5 83 10.0 84 6.5 85 3.5 86 1.5 87 0.5 88 1.5 89 1.5 90 0.0 91 0.0 92 1.0 93 1.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.023509929548440542 2 0.023592276061745063 3 4.117325665226014E-5 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 3.0879942489195106E-4 18-19 0.0 20-21 0.0 22-23 0.0 24-25 6.175988497839021E-5 26-27 2.4703953991356086E-4 28-29 1.4410639828291052E-4 30-31 5.352523364793819E-4 32-33 0.0022027692308959177 34-35 0.004199672178530535 36-37 0.10635052193278796 38-39 0.09825997700061884 40-41 0.1080180388272045 42-43 0.10929440978342454 44-45 0.10171853055940869 46-47 0.09648952696457165 48-49 0.09721005895598621 50-51 0.09648952696457165 52-53 0.07600583178007222 54-55 0.06433321351915648 56-57 0.035491347234248245 58-59 0.01455474622657396 60-61 0.009572782171650484 62-63 0.0019351430626562266 64-65 0.005620149533033509 66-67 0.16827509993778722 68-69 0.44514466429591054 70-71 0.41883495329511633 72-73 0.19763163193084868 74-75 0.029480051763018267 76-77 0.03503844141107339 78-79 0.0055583896480551195 80-81 0.0015439971244597553 82-83 4.117325665226014E-5 84-85 4.7349245150099165E-4 86-87 0.0012146110712416742 88-89 2.676261682396909E-4 90-91 8.234651330452029E-4 92-93 0.0027174349390491695 94-95 0.0852286412701785 96-97 0.01686044859910053 98-99 0.019104391086648707 100-101 0.010108034508129865 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2428761.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.71660872759407 #Duplication Level Percentage of deduplicated Percentage of total 1 72.88291558042768 38.42140143579676 2 14.492297305226431 15.279695332071755 3 5.305905009549793 8.391279550026534 4 2.537556881047375 5.350855728887539 5 1.401199632189194 3.693324637968324 6 0.8237361690051924 2.605474638972842 7 0.5170560494902605 1.908020901584951 8 0.3643743119472785 1.5366862426648789 9 0.25770775474477825 1.2226930985652524 >10 1.2804984746468309 11.980593304197793 >50 0.08565137797841581 3.1100290127504766 >100 0.04812739259638742 4.658412841457672 >500 0.0021914134793428884 0.7311011702744886 >1k 6.261181369530821E-4 0.5478307753317886 >5k 1.5652953423827052E-4 0.5626013294490336 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7462 0.3072348411391652 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6174 0.25420368657105413 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4260 0.1753980733386282 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 4.117325665226014E-5 0.0 0.0 0.0 0.0 3 4.117325665226014E-5 0.0 0.0 0.0 0.0 4 4.117325665226014E-5 0.0 0.0 0.0 0.0 5 8.234651330452028E-5 0.0 0.0 0.0 0.0 6 8.234651330452028E-5 0.0 0.0 0.0 0.0 7 2.0586628326130072E-4 1.2351976995678043E-4 0.0 0.0 0.0 8 2.4703953991356086E-4 1.2351976995678043E-4 0.0 0.0 0.0 9 2.4703953991356086E-4 1.2351976995678043E-4 0.0 0.0 0.0 10-11 2.4703953991356086E-4 1.6469302660904056E-4 0.0 0.0 0.0 12-13 2.676261682396909E-4 1.6469302660904056E-4 0.0 2.058662832613007E-5 0.0 14-15 3.705593098703413E-4 2.0586628326130072E-4 0.0 4.117325665226014E-5 0.0 16-17 3.911459381964714E-4 2.4703953991356086E-4 0.0 8.234651330452028E-5 0.0 18-19 5.146657081532518E-4 2.4703953991356086E-4 0.0 1.2351976995678043E-4 0.0 20-21 5.76425593131642E-4 2.4703953991356086E-4 0.0 2.676261682396909E-4 0.0 22-23 5.76425593131642E-4 2.4703953991356086E-4 0.0 4.940790798271217E-4 0.0 24-25 6.587721064361623E-4 2.4703953991356086E-4 0.0 0.001441063982829105 0.0 26-27 6.587721064361623E-4 2.4703953991356086E-4 0.0 0.00275860819570143 0.0 28-29 7.617052480668126E-4 2.4703953991356086E-4 0.0 0.007884678648907819 0.0 30-31 7.822918763929428E-4 2.88212796565821E-4 0.0 0.026906723222252005 0.0 32-33 8.234651330452029E-4 2.88212796565821E-4 0.0 0.06733886125477147 0.0 34-35 9.058116463497232E-4 2.88212796565821E-4 0.0 0.12852232064003005 0.0 36-37 0.0010293314163065037 2.88212796565821E-4 0.0 0.23209364774879043 0.0 38-39 0.0013587174695245847 2.88212796565821E-4 0.0 0.38037913158190534 0.0 40-41 0.0013587174695245847 2.88212796565821E-4 0.0 0.547295514050168 0.0 42-43 0.0015028238678074953 3.0879942489195106E-4 0.0 0.7142530697750828 0.0 44-45 0.001729276779394926 3.499726815442112E-4 0.0 0.9193988210449691 0.0 46-47 0.0018116232926994463 3.705593098703413E-4 0.0 1.1555686212023333 0.0 48-49 0.001976316319308487 3.705593098703413E-4 0.0 1.4068490065510768 0.0 50-51 0.001976316319308487 3.705593098703413E-4 0.0 1.6669198821950781 0.0 52-53 0.001976316319308487 3.705593098703413E-4 0.0 1.919044319305193 0.0 54-55 0.001976316319308487 5.146657081532518E-4 0.0 2.195543324353446 0.0 56-57 0.0020998360892652674 5.76425593131642E-4 0.0 2.5114451360179118 0.0 58-59 0.0020998360892652674 6.587721064361623E-4 0.0 2.8267499354609202 0.0 60-61 0.0020998360892652674 6.793587347622924E-4 0.0 3.149465921101335 0.0 62-63 0.0020998360892652674 7.205319914145526E-4 0.0 3.456289029673978 0.0 64-65 0.0021410093459175274 7.617052480668126E-4 0.0 3.7754641152422983 0.0 66-67 0.002264529115874308 8.234651330452029E-4 0.0 4.1176962245358855 0.0 68-69 0.002305702372526568 8.234651330452029E-4 0.0 4.5029543870310835 0.0 70-71 0.002305702372526568 9.058116463497232E-4 0.0 4.947707905388797 0.0 72-73 0.002346875629178828 9.881581596542434E-4 0.0 5.397896293624609 0.0 74-75 0.0024086355141572187 9.881581596542434E-4 0.0 5.854898855836371 0.0 76-77 0.0024292221424833484 9.881581596542434E-4 0.0 6.342781360537327 0.0 78-79 0.0024498087708094782 9.881581596542434E-4 0.0 6.874183997519722 0.0 80-81 0.0027586081957014295 0.0010499180446326337 0.0 7.4490861801552315 0.0 82-83 0.0027586081957014295 0.0010705046729587637 0.0 8.013756808512653 0.0 84-85 0.0028203680806798198 0.0010705046729587637 0.0 8.566775405237486 0.0 86-87 0.00290271459398434 0.001111677929611024 0.0 9.132516538267865 0.0 88-89 0.00292330122231047 0.001173437814589414 2.058662832613007E-5 9.728869987619202 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6500 0.0 37.23955 1 GTATCAA 9950 0.0 31.196241 1 ATCAACG 12105 0.0 25.470985 3 TATCAAC 12190 0.0 25.377472 2 TCAACGC 12155 0.0 25.327183 4 CAACGCA 12320 0.0 24.987982 5 GTACATG 9345 0.0 24.886532 1 AACGCAG 12660 0.0 24.316898 6 TACATGG 9445 0.0 24.012117 2 ACATGGG 9775 0.0 22.176683 3 ACGCAGA 14185 0.0 21.6692 7 CGCAGAG 14385 0.0 21.301971 8 GAGTACT 7300 0.0 21.118238 12-13 AGAGTAC 12160 0.0 19.797012 10-11 GCAGAGT 15395 0.0 19.50389 9 GTACTTT 7885 0.0 19.37097 14-15 AGTACTT 7680 0.0 19.115984 12-13 GTGTAGC 1505 0.0 18.291069 1 CATGGGG 4535 0.0 18.09531 4 CATGGGA 5240 0.0 17.833317 4 >>END_MODULE