FastQCFastQC Report
Fri 27 May 2016
SRR939759_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939759_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1477253
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34010.23022461284559922No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27070.18324552395561222No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17190.1163646308384549No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA19850.042.3031851
GTATCAA34650.041.485821
TATCAAC44750.032.6526152
ATCAACG45050.032.3298653
TCAACGC45400.031.871634
CAACGCA46750.030.8497945
TACATGG40800.030.4650172
AACGCAG48600.029.675476
GTACATG43100.028.3989811
TACCGTA856.4542243E-427.9068857
ACATGGG42750.027.7436873
ACGCAGA53600.026.3761737
CGCAGAG55050.025.7684848
CGCTATA1401.7969205E-523.7208521
TAGTACG1401.7969205E-523.7208524
CATGGGG26900.022.5744924
GCAGAGT61350.022.5036759
AGAGTAC52000.021.21263710-11
GAGTACT37700.020.26059512-13
GTATAGC6800.020.2324921
Produced by FastQC (version 0.11.5)