##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939759_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1477253 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.757353682815335 31.0 28.0 33.0 16.0 34.0 2 29.599512067330377 31.0 30.0 33.0 25.0 34.0 3 29.866437231807957 31.0 30.0 34.0 25.0 34.0 4 33.62373540618973 35.0 33.0 37.0 28.0 37.0 5 33.57598190695839 35.0 33.0 37.0 28.0 37.0 6 33.60293598997599 35.0 33.0 37.0 28.0 37.0 7 33.50562902901534 35.0 33.0 37.0 28.0 37.0 8 33.369419456247506 35.0 33.0 37.0 26.0 37.0 9 34.64917045353775 37.0 34.0 39.0 27.0 39.0 10-11 34.461494070413124 37.0 33.5 39.0 27.0 39.0 12-13 34.318362189821244 37.0 33.0 39.0 27.0 39.0 14-15 34.98829652063662 38.0 33.0 40.0 25.5 41.0 16-17 34.789162722972975 38.0 33.0 40.0 25.0 41.0 18-19 34.59935941913809 38.0 33.0 40.0 25.0 41.0 20-21 34.77978585929424 38.0 33.0 40.0 25.0 41.0 22-23 34.67674156017961 38.0 33.0 40.0 25.0 41.0 24-25 34.455011091532725 38.0 33.0 40.0 24.0 41.0 26-27 34.33312371002123 38.0 33.0 40.0 23.5 41.0 28-29 34.3754001514974 38.0 33.0 40.0 24.0 41.0 30-31 34.16154781882318 38.0 33.0 40.0 23.0 41.0 32-33 33.82022036848123 38.0 32.5 40.0 20.5 41.0 34-35 33.63126458365629 38.0 32.0 40.0 19.0 41.0 36-37 33.41367660109677 37.5 31.5 40.0 18.5 41.0 38-39 33.175527482428535 37.0 31.0 40.0 17.0 41.0 40-41 32.924167018107255 37.0 31.0 40.0 16.0 41.0 42-43 32.5375328396693 37.0 30.5 40.0 15.0 41.0 44-45 32.189987429370596 37.0 30.0 40.0 11.5 41.0 46-47 31.80440994196661 36.5 30.0 39.0 9.0 41.0 48-49 31.440431327606035 36.0 30.0 39.0 8.5 40.0 50-51 30.82157423271437 35.5 28.5 38.5 7.5 40.0 52-53 30.982779523886563 35.5 29.0 39.0 7.0 40.0 54-55 31.058573243716545 36.0 29.0 39.0 2.0 40.0 56-57 30.740265546930686 35.5 28.5 39.0 2.0 40.0 58-59 30.30936203886538 35.0 28.0 39.0 2.0 40.0 60-61 29.8793134960633 35.0 27.5 38.0 2.0 40.0 62-63 29.418459464966393 34.0 26.5 38.0 2.0 40.0 64-65 28.858017211675996 34.0 26.0 38.0 2.0 40.0 66-67 28.232454765703643 34.0 25.5 37.0 2.0 39.5 68-69 27.655747864448404 33.0 24.0 36.5 2.0 39.0 70-71 27.069223755172608 32.5 22.5 36.0 2.0 39.0 72-73 26.397816420071578 32.0 21.5 35.5 2.0 38.0 74-75 26.024122814440048 32.0 20.5 35.0 2.0 38.0 76-77 25.44700738465246 31.0 18.5 35.0 2.0 37.0 78-79 24.76255353686877 31.0 16.0 34.5 2.0 36.5 80-81 24.01881972823883 30.5 8.5 34.0 2.0 36.0 82-83 23.24527078300061 30.0 4.5 34.0 2.0 35.5 84-85 22.441973040501527 29.0 2.0 34.0 2.0 35.0 86-87 21.50048569879364 29.0 2.0 34.0 2.0 35.0 88-89 20.497152823517702 27.5 2.0 33.5 2.0 35.0 90-91 19.075224758386003 25.5 2.0 32.5 2.0 34.5 92-93 17.54786316223423 21.5 2.0 32.0 2.0 34.0 94-95 16.988708095363492 19.5 2.0 31.0 2.0 34.0 96-97 16.622666530377668 18.5 2.0 31.0 2.0 34.0 98-99 15.698851855437084 2.0 2.0 31.0 2.0 34.0 100-101 14.097879983997323 2.0 2.0 30.0 2.0 33.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.6351167714912087 1101 2 0.8651324058840579 1101 3 0.889352893978085 1101 4 0.5693510323898323 1101 5 0.872643749181492 1101 6 0.8567345829981718 1101 7 0.9507046032946391 1101 8 1.0303599361216982 1101 9 1.019765947746329 1101 10-11 0.9681813124687437 1101 12-13 1.024438366854639 1101 14-15 0.9972908002399379 1101 16-17 0.8317958406217372 1101 18-19 0.9372094107333453 1101 20-21 0.9758070479822791 1101 22-23 1.1541311069104339 1101 24-25 1.258500056926323 1101 26-27 1.0583109785143208 1101 28-29 1.080770633576428 1101 30-31 1.2644229101558082 1101 32-33 1.256030062210037 1101 34-35 1.1236637992915703 1101 36-37 0.7937662411630697 1101 38-39 1.1445764821588043 1101 40-41 1.296560194818582 1101 42-43 1.3679067393200555 1101 44-45 1.20247565666876 1101 46-47 1.164234491421066 1101 48-49 1.140447587297981 1101 50-51 1.1525718355498817 1101 52-53 0.9652372541317824 1101 54-55 1.0851264162553456 1101 56-57 0.939784202052877 1101 58-59 1.1394752130835393 1101 60-61 0.8064467537794862 1101 62-63 0.5409531315283047 1101 64-65 0.958835310671386 1101 66-67 1.1347639631382016 1101 68-69 0.8734475534609061 1101 70-71 0.5858814410342141 1101 72-73 0.7583517034614395 1101 74-75 0.670461265627555 1101 76-77 0.41852747064583795 1101 78-79 0.30885775202535726 1101 80-81 -0.058250992868014606 1101 82-83 -0.28587201081406377 1101 84-85 -0.40142459203390146 1101 86-87 -0.5066575254499668 1101 88-89 -4.641722930892671 1101 90-91 -8.167330757261851 1101 92-93 -10.600326067126142 1101 94-95 -8.590165846668288 1101 96-97 -5.313017842959404 1101 98-99 -3.881782109156031 1101 100-101 -2.9579283821721205 1102 1 1.8871905708751555 1102 2 1.3571935737039134 1102 3 1.1205308049714269 1102 4 1.0601525721704377 1102 5 1.1389862762705505 1102 6 1.0346244061209902 1102 7 1.1640289628073006 1102 8 1.1095383199166022 1102 9 1.5121199702643864 1102 10-11 1.3566804144986122 1102 12-13 1.23986078329402 1102 14-15 1.6117766991097966 1102 16-17 1.600259359041594 1102 18-19 1.6355540760145288 1102 20-21 1.521932986087613 1102 22-23 1.545882483856765 1102 24-25 1.6579737123527778 1102 26-27 1.729864290418682 1102 28-29 1.3868015879651878 1102 30-31 1.6471950268601674 1102 32-33 1.7202087282745282 1102 34-35 1.7746815575770611 1102 36-37 1.7319553759009239 1102 38-39 1.5223440472660528 1102 40-41 1.5420628463885322 1102 42-43 1.5623415297525298 1102 44-45 1.6827476587010963 1102 46-47 1.4428438373671462 1102 48-49 1.4874124835462617 1102 50-51 1.6424868724037047 1102 52-53 1.7491920804124952 1102 54-55 1.7546465050291324 1102 56-57 1.9877422462068992 1102 58-59 1.6877750680675376 1102 60-61 1.713008022157883 1102 62-63 1.8678191099276233 1102 64-65 1.7961620236067404 1102 66-67 1.8128050651186776 1102 68-69 1.4821438384402832 1102 70-71 1.6547750677397453 1102 72-73 1.619363922206258 1102 74-75 1.3718116609813968 1102 76-77 1.4511891493736506 1102 78-79 1.5046377334661294 1102 80-81 1.5218112475762773 1102 82-83 1.293780056634258 1102 84-85 1.1318360274772807 1102 86-87 1.077342375755677 1102 88-89 0.392526514271168 1102 90-91 -5.662149787171842 1102 92-93 -10.066460332184342 1102 94-95 -8.10379152896681 1102 96-97 -4.4391985237230855 1102 98-99 -2.6994950550978754 1102 100-101 -1.794914607416196 1103 1 2.0441744176385157 1103 2 1.049313527270737 1103 3 0.9249064522366801 1103 4 0.7946848330333154 1103 5 0.8312323291821997 1103 6 0.6135921085909288 1103 7 0.5908220328881271 1103 8 0.8002899614438874 1103 9 0.6342565760992969 1103 10-11 0.7606199905544102 1103 12-13 0.5963550337004904 1103 14-15 0.7726271452868403 1103 16-17 0.6992001692611822 1103 18-19 0.6423334808613959 1103 20-21 0.6072143754340971 1103 22-23 0.45243839773717553 1103 24-25 0.5568270579204366 1103 26-27 0.7509624547341858 1103 28-29 0.42453100381221276 1103 30-31 0.6627636007570672 1103 32-33 0.698692468613622 1103 34-35 0.48147106825794594 1103 36-37 0.48669975195957704 1103 38-39 0.43418227164418255 1103 40-41 0.1880449724771367 1103 42-43 0.16548654578772215 1103 44-45 -0.04550333444303334 1103 46-47 0.21303292378186356 1103 48-49 0.07467010003612273 1103 50-51 0.09171042239538707 1103 52-53 0.1737374967460248 1103 54-55 -0.026210943685995858 1103 56-57 -0.007521203048398206 1103 58-59 0.16843625790875905 1103 60-61 -0.1281839386064405 1103 62-63 -0.04585940471152483 1103 64-65 -0.2846289770139201 1103 66-67 -0.04804855590002788 1103 68-69 -0.29239522820992647 1103 70-71 -0.31267140264121807 1103 72-73 -0.1644991178074804 1103 74-75 -0.5147827713300828 1103 76-77 -0.5498627964018432 1103 78-79 -0.8177904659246593 1103 80-81 -0.8193143098096556 1103 82-83 -0.9857245070888432 1103 84-85 -0.9639402257802878 1103 86-87 -0.7200222373697436 1103 88-89 -1.7011386928626564 1103 90-91 -6.9299716043260045 1103 92-93 -10.067995658781644 1103 94-95 -8.849797488725915 1103 96-97 -5.842878746516481 1103 98-99 -4.415393678379214 1103 100-101 -3.897244002157226 1104 1 2.081367901948653 1104 2 1.3519560701029647 1104 3 0.9663125246965016 1104 4 0.9264084507755825 1104 5 0.7995126246162343 1104 6 0.9998290725979615 1104 7 0.9038305438671017 1104 8 1.1012857391625488 1104 9 1.036373137774369 1104 10-11 1.2291277169235784 1104 12-13 1.0681478536322686 1104 14-15 0.9998764228750048 1104 16-17 0.8679864132617254 1104 18-19 0.9485490408710291 1104 20-21 0.9610082357681691 1104 22-23 1.1251846684622038 1104 24-25 1.1518960608610556 1104 26-27 1.0282678989676484 1104 28-29 0.9439110992434578 1104 30-31 1.084943448186273 1104 32-33 0.9242754152066581 1104 34-35 1.0816954117328734 1104 36-37 0.9759588010002886 1104 38-39 0.8746012902554625 1104 40-41 0.8525179268642304 1104 42-43 0.9027503236686485 1104 44-45 0.6985993473033645 1104 46-47 0.680713498020733 1104 48-49 0.37875261994934206 1104 50-51 0.3608762803987844 1104 52-53 0.4489544177511995 1104 54-55 0.5212393706425864 1104 56-57 0.6103117158113491 1104 58-59 0.6583047213336641 1104 60-61 0.8102104600227271 1104 62-63 0.7911650591025428 1104 64-65 0.6745765189524597 1104 66-67 0.7061610704418975 1104 68-69 0.6901371066531325 1104 70-71 0.5865004041081825 1104 72-73 0.5949773207425473 1104 74-75 0.42230735486446846 1104 76-77 0.4550611641203126 1104 78-79 0.1659070261601343 1104 80-81 -0.13532358080644613 1104 82-83 -0.4100314709989412 1104 84-85 -0.270941652115102 1104 86-87 -0.19191055316379035 1104 88-89 -0.6666397355984905 1104 90-91 -3.3703414389781514 1104 92-93 -7.807555824944446 1104 94-95 -5.7702079701507145 1104 96-97 -3.2544327047302204 1104 98-99 -2.533303065730177 1104 100-101 -2.0805032543959054 1105 1 1.969886782567503 1105 2 1.173450595520535 1105 3 1.0055933351686086 1105 4 0.918605812937102 1105 5 0.8673847940425645 1105 6 0.9629930636761443 1105 7 0.7526873681472992 1105 8 0.7373690788430451 1105 9 0.8210125087353362 1105 10-11 0.7962461349696639 1105 12-13 0.7556159357366496 1105 14-15 0.9579608122302545 1105 16-17 0.7731554579286737 1105 18-19 0.8318865570875147 1105 20-21 0.7816191531347911 1105 22-23 0.9285618991771329 1105 24-25 0.8680934746322748 1105 26-27 0.7623582717187247 1105 28-29 0.9888589880112235 1105 30-31 1.0181265723130153 1105 32-33 1.2411152865320147 1105 34-35 1.1710209852977087 1105 36-37 1.076488882589409 1105 38-39 1.0411813858083363 1105 40-41 1.0966326519031284 1105 42-43 0.8849337387242784 1105 44-45 0.7852641077406943 1105 46-47 0.8618192275717398 1105 48-49 0.796667388190567 1105 50-51 0.9093931465744092 1105 52-53 0.5261365196728711 1105 54-55 0.8196119169446057 1105 56-57 1.06900376779231 1105 58-59 1.169361267670645 1105 60-61 1.1084814517489434 1105 62-63 1.0577391612706109 1105 64-65 0.9116891808518872 1105 66-67 0.6863156831214852 1105 68-69 0.7789126623002574 1105 70-71 0.5972601946560339 1105 72-73 0.9395297293548737 1105 74-75 0.8058550557562718 1105 76-77 0.7981822359039974 1105 78-79 0.4621115487516576 1105 80-81 0.10885274290958336 1105 82-83 0.08393997468455083 1105 84-85 -0.361290679376264 1105 86-87 0.021644776807423938 1105 88-89 -0.09424193633609335 1105 90-91 -3.248530054959925 1105 92-93 -7.588592921915332 1105 94-95 -5.385640353324451 1105 96-97 -2.7549055161978977 1105 98-99 -1.9320558590012702 1105 100-101 -1.560911337307724 1106 1 1.600589850141695 1106 2 0.9863932601245544 1106 3 0.836300124516697 1106 4 0.5241158991892121 1106 5 0.8054339087256039 1106 6 0.7647444521181725 1106 7 0.850752682340925 1106 8 -0.4131046882826226 1106 9 -0.2758926893585283 1106 10-11 -0.7063343765489876 1106 12-13 -0.18439176245883004 1106 14-15 0.7534275447135457 1106 16-17 0.5976946313128977 1106 18-19 0.5429104023949165 1106 20-21 0.7063519077322837 1106 22-23 0.597304923911743 1106 24-25 0.5987318029289739 1106 26-27 0.7908981561576454 1106 28-29 0.6389987749004931 1106 30-31 0.6169727244640342 1106 32-33 0.7182834279515902 1106 34-35 0.9008057541339269 1106 36-37 0.8050564844497075 1106 38-39 0.6337078679435777 1106 40-41 0.4227043636301957 1106 42-43 0.5698703975605284 1106 44-45 0.5306413952237321 1106 46-47 0.6010259608483715 1106 48-49 0.61886576167743 1106 50-51 0.6327181891666847 1106 52-53 0.4765862421803462 1106 54-55 0.5759184872956773 1106 56-57 0.6527023001891266 1106 58-59 0.5191201114134394 1106 60-61 0.3492900169218558 1106 62-63 0.4950493079525735 1106 64-65 0.401376684065589 1106 66-67 0.35651460703912363 1106 68-69 -0.006125267962517 1106 70-71 -0.05158505153686477 1106 72-73 -0.04085995016611221 1106 74-75 -0.06309540890229925 1106 76-77 -0.08924683614513995 1106 78-79 0.13666935549071368 1106 80-81 -0.3230823787361068 1106 82-83 -0.4070474560035322 1106 84-85 -0.29569735271273956 1106 86-87 -0.42003233286297004 1106 88-89 -0.887694748405611 1106 90-91 -4.050771150493455 1106 92-93 -8.898360325738082 1106 94-95 -6.592066093896243 1106 96-97 -3.8095294040118777 1106 98-99 -2.852919682722389 1106 100-101 -2.1788624322193257 1107 1 1.6255290186466809 1107 2 1.2347467476806386 1107 3 0.9966076861630988 1107 4 0.802598047475513 1107 5 0.6333702432454942 1107 6 0.5975024489303138 1107 7 0.4161795594448563 1107 8 0.38367924203503634 1107 9 0.625227846217804 1107 10-11 0.5904211441397038 1107 12-13 0.620609777642116 1107 14-15 0.619549660263985 1107 16-17 0.7231794640599318 1107 18-19 0.5834469556191095 1107 20-21 0.7166015536087542 1107 22-23 0.6268918080333563 1107 24-25 0.7055796698642496 1107 26-27 0.7210461055306965 1107 28-29 0.613189873017383 1107 30-31 0.6496764439323996 1107 32-33 0.8716226558675899 1107 34-35 0.8261724127927366 1107 36-37 0.5597124239062907 1107 38-39 0.6797628669916023 1107 40-41 0.6661753823197429 1107 42-43 0.6628703148237136 1107 44-45 0.5024288892053832 1107 46-47 0.4622282264415212 1107 48-49 0.37515724845798104 1107 50-51 0.6034046854220954 1107 52-53 0.5483260378901953 1107 54-55 0.5253795018085121 1107 56-57 0.2979236012305151 1107 58-59 0.3546647439373771 1107 60-61 0.29568727723679444 1107 62-63 0.13642683603573857 1107 64-65 0.26794836953411405 1107 66-67 0.03737304634369565 1107 68-69 0.4445015381671844 1107 70-71 0.38539067866536314 1107 72-73 0.6010870165328193 1107 74-75 0.4614724407678992 1107 76-77 0.6303244575695537 1107 78-79 0.6834575223319916 1107 80-81 0.5045167783452165 1107 82-83 0.2563303392298302 1107 84-85 0.2781193363991932 1107 86-87 0.36947001018379666 1107 88-89 -0.0690692959698076 1107 90-91 -2.946956634468929 1107 92-93 -3.966873884108903 1107 94-95 -2.509615868064614 1107 96-97 -1.0605077917604504 1107 98-99 -0.7399891647002175 1107 100-101 -0.27328781507645594 1108 1 1.5786895722975096 1108 2 1.014421500195418 1108 3 -0.7577520991230173 1108 4 -0.35035619902073734 1108 5 0.4399855861444806 1108 6 0.729363521990777 1108 7 0.7492252094001444 1108 8 0.6522291159779385 1108 9 0.7053287269940327 1108 10-11 0.7513130825406478 1108 12-13 0.6836367888736135 1108 14-15 0.5936956706205905 1108 16-17 0.4057424289859526 1108 18-19 0.2001939015975296 1108 20-21 0.3584557117377898 1108 22-23 0.15090978685505974 1108 24-25 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90-91 1.1499320412778253 1108 92-93 -1.5496915958106712 1108 94-95 -1.533973011987328 1108 96-97 -0.38812965207005945 1108 98-99 -0.017218023482046263 1108 100-101 0.3559733578468496 1109 1 1.7947605718920059 1109 2 1.1183045307465385 1109 3 0.8749337741793006 1109 4 0.6919791157055712 1109 5 0.3967120405272837 1109 6 0.6282163896295003 1109 7 0.4424971214731883 1109 8 0.6115646286513652 1109 9 0.40004161719487996 1109 10-11 0.5828093513336228 1109 12-13 0.4679261299018975 1109 14-15 0.49226007357941626 1109 16-17 0.6064221755367782 1109 18-19 0.4178992140753053 1109 20-21 0.4367136233765052 1109 22-23 -0.08476451134554708 1109 24-25 0.2747857426364817 1109 26-27 0.6655675822459699 1109 28-29 0.21729998411225182 1109 30-31 0.28568079555406456 1109 32-33 0.6582655703019427 1109 34-35 0.4761458244729866 1109 36-37 0.5528318098933624 1109 38-39 0.5213775756500851 1109 40-41 0.3150198982572263 1109 42-43 0.49733210076114887 1109 44-45 0.4756551159239777 1109 46-47 0.34989501275562773 1109 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98-99 1.8193244867186973 2311 100-101 1.888563017827007 2312 1 1.1839450917229932 2312 2 0.5636204936447875 2312 3 -0.17151298684431993 2312 4 -0.5638677711794386 2312 5 -0.23552645507734127 2312 6 -0.38803810053416044 2312 7 0.009103593562841183 2312 8 0.29937483593177916 2312 9 0.24702348455573997 2312 10-11 0.24115353141702656 2312 12-13 0.23055800097606038 2312 14-15 0.048321941096624244 2312 16-17 -0.19251836082035112 2312 18-19 -0.23459045708250414 2312 20-21 0.3792879969034004 2312 22-23 0.49721053526617 2312 24-25 0.43009068289141084 2312 26-27 0.4704972835792418 2312 28-29 0.494244093056615 2312 30-31 0.36846944614018895 2312 32-33 0.522359505112 2312 34-35 0.43307132782081936 2312 36-37 0.46489026899036645 2312 38-39 0.3888519831830095 2312 40-41 0.3883349386931272 2312 42-43 0.6052988921165863 2312 44-45 0.4007750164856674 2312 46-47 0.296460794874708 2312 48-49 0.492297270339467 2312 50-51 0.5618981290076626 2312 52-53 0.36646652203146957 2312 54-55 0.35667832688334045 2312 56-57 0.5763967517897797 2312 58-59 0.2600518552777338 2312 60-61 0.315145023300893 2312 62-63 0.11525524841430723 2312 64-65 0.284681192299427 2312 66-67 0.6085333898080734 2312 68-69 0.5097952873082576 2312 70-71 0.6280088003200035 2312 72-73 0.45578127362422194 2312 74-75 0.36855063763345797 2312 76-77 0.6758311674756605 2312 78-79 0.8139276708797993 2312 80-81 0.6620273398213072 2312 82-83 0.9305001010143279 2312 84-85 1.174613747339226 2312 86-87 1.5014969390475486 2312 88-89 1.7084427468793528 2312 90-91 2.5272041141420623 2312 92-93 3.796869420709484 2312 94-95 3.6306447286610144 2312 96-97 2.655943123924434 2312 98-99 2.4838547842477414 2312 100-101 2.4627779079139174 2313 1 -7.593256252107302 2313 2 -2.99245961153553 2313 3 -1.8836885254668019 2313 4 -1.146865568334384 2313 5 -0.957959287266057 2313 6 -0.5574067025650749 2313 7 -0.41914117823568375 2313 8 -0.3921976225001842 2313 9 -0.3139774667446389 2313 10-11 -0.5403981943556246 2313 12-13 -0.5591062468803614 2313 14-15 -0.7312821472826556 2313 16-17 -0.8456153018049619 2313 18-19 -0.9181246643901346 2313 20-21 -0.5934913014098626 2313 22-23 -1.4474643159548748 2313 24-25 -0.5519286338789726 2313 26-27 -0.593363889775496 2313 28-29 -0.7366082215109273 2313 30-31 -0.3189157894251693 2313 32-33 -0.5572589633892093 2313 34-35 -0.45614365974274307 2313 36-37 -0.24365547401947651 2313 38-39 -0.199694012664132 2313 40-41 -0.311314486576066 2313 42-43 -0.28611854544049464 2313 44-45 -0.4829320683317704 2313 46-47 -1.322475060300082 2313 48-49 -0.7304109174807287 2313 50-51 -0.8018032037610006 2313 52-53 -1.1406307002346487 2313 54-55 -0.8740542850208719 2313 56-57 -0.8908884708176714 2313 58-59 -0.5438190839180521 2313 60-61 -0.28328651625708545 2313 62-63 -0.41392770782271526 2313 64-65 -0.5702217971856989 2313 66-67 -0.14077762852579312 2313 68-69 -0.022785565322358536 2313 70-71 0.0790990031903398 2313 72-73 0.04493328889559578 2313 74-75 0.20790057711052512 2313 76-77 0.5131888279720407 2313 78-79 0.3258935915710559 2313 80-81 0.24043534970724423 2313 82-83 0.5856454192574923 2313 84-85 0.5219802097344122 2313 86-87 1.0650795963044537 2313 88-89 1.6520386938972926 2313 90-91 2.4758548320419926 2313 92-93 3.23075206943313 2313 94-95 3.0794755931433784 2313 96-97 2.0920531113141756 2313 98-99 1.679941968872706 2313 100-101 1.4114037371412778 2314 1 0.5079923126441308 2314 2 0.047676597004663535 2314 3 -1.3171388711444898 2314 4 -0.22252042333165178 2314 5 -0.1879876105871645 2314 6 -3.2890874902912017 2314 7 -2.894750478808813 2314 8 -2.9017141122272996 2314 9 -3.5345571041606547 2314 10-11 -2.6193104748864258 2314 12-13 -1.448620324902663 2314 14-15 -1.193187361599577 2314 16-17 -2.1830160524379423 2314 18-19 -3.4669082963038917 2314 20-21 -2.875253331919147 2314 22-23 -4.068142735825376 2314 24-25 -3.0420463283216606 2314 26-27 -1.6694872813632813 2314 28-29 -1.5678684631361364 2314 30-31 -1.7578913345760228 2314 32-33 -1.9549312216381374 2314 34-35 -1.3461201841919106 2314 36-37 -1.7265279037674013 2314 38-39 -3.068140793566034 2314 40-41 -2.368957276553118 2314 42-43 -1.488785387503878 2314 44-45 -1.5916550909368326 2314 46-47 -1.0517463762788815 2314 48-49 -2.011722275879549 2314 50-51 -2.9845753082129924 2314 52-53 -1.7806027585637985 2314 54-55 -2.2393721428423454 2314 56-57 -1.4517145893371257 2314 58-59 -0.8668580106200885 2314 60-61 -1.3222178874208907 2314 62-63 -0.8669851323818243 2314 64-65 -1.311729266974428 2314 66-67 -0.5430305018650046 2314 68-69 -0.8004079085899676 2314 70-71 -0.8295193092009505 2314 72-73 -1.7909782137183043 2314 74-75 -2.090812002893923 2314 76-77 -1.077485994641794 2314 78-79 -1.9684620853546946 2314 80-81 -0.7733893217893275 2314 82-83 -0.14934699645870353 2314 84-85 -1.2262489736802031 2314 86-87 0.20584662399602038 2314 88-89 1.4326535405794552 2314 90-91 2.5051393839764735 2314 92-93 3.415367463417997 2314 94-95 3.152455039247055 2314 96-97 2.3540233279778384 2314 98-99 2.1928186048936684 2314 100-101 2.277893545455406 2315 1 -3.2650425675858123 2315 2 -2.530000449569574 2315 3 -3.0412813844700466 2315 4 -4.216825993242221 2315 5 -4.299853385438158 2315 6 -3.3584837428935614 2315 7 -6.153869195617961 2315 8 -3.3783039508440993 2315 9 -3.0757888138185443 2315 10-11 -4.4817164085121135 2315 12-13 -3.6470714596052147 2315 14-15 -2.554584342616508 2315 16-17 -3.0799281652831354 2315 18-19 -2.6143851765239887 2315 20-21 -1.8580297726296422 2315 22-23 -1.475491356021159 2315 24-25 -2.8090767100994825 2315 26-27 -4.2949394643922005 2315 28-29 -3.7686869451792404 2315 30-31 -3.502958540663208 2315 32-33 -4.13521874852303 2315 34-35 -3.995361501047327 2315 36-37 -3.159572824306231 2315 38-39 -1.801523836271798 2315 40-41 -1.9363380847979812 2315 42-43 -3.1119514315729617 2315 44-45 -6.842170641111309 2315 46-47 -3.520463923687352 2315 48-49 -2.5568667529714055 2315 50-51 -3.846132245131308 2315 52-53 -3.591128841219163 2315 54-55 -2.0385585825192862 2315 56-57 -1.6196461380547724 2315 58-59 -3.0343269176537184 2315 60-61 -3.377350995339448 2315 62-63 -5.120507853502403 2315 64-65 -4.52513258716677 2315 66-67 -4.168600806174954 2315 68-69 -2.5768313930693196 2315 70-71 -3.8487804605881344 2315 72-73 -2.9900051896658546 2315 74-75 -2.1094948521760912 2315 76-77 -4.045743361226268 2315 78-79 -2.491382535593644 2315 80-81 -1.8875535399002707 2315 82-83 -1.5768180690491533 2315 84-85 -2.931383804200774 2315 86-87 -2.5670553697927687 2315 88-89 -2.3096181285234643 2315 90-91 -1.4348623878427418 2315 92-93 -1.1837732042625433 2315 94-95 -1.2628618456978966 2315 96-97 -1.5499293053884706 2315 98-99 -1.8111802926410423 2315 100-101 -1.8870455974762628 2316 1 -7.2369615752242495 2316 2 -6.112363520482262 2316 3 -3.2229181952297132 2316 4 -3.9625961437365547 2316 5 -2.0166124107093566 2316 6 -2.549934958685842 2316 7 -3.4269073208813516 2316 8 -2.5016246318487774 2316 9 -6.830280293500863 2316 10-11 -6.805081607867514 2316 12-13 -5.979534308847555 2316 14-15 -4.743995861424537 2316 16-17 -4.632155513420031 2316 18-19 -3.7607060355050805 2316 20-21 -2.740742831491339 2316 22-23 -3.196176046315532 2316 24-25 -2.9498659942705885 2316 26-27 -3.9479841461708602 2316 28-29 -3.8888489085645936 2316 30-31 -6.5000425401957145 2316 32-33 -4.521288626815291 2316 34-35 -5.4236680433535795 2316 36-37 -3.7351812781373575 2316 38-39 -6.0929905219484795 2316 40-41 -3.522514797432997 2316 42-43 -4.434423354217721 2316 44-45 -3.1413606302047974 2316 46-47 -4.099396705342297 2316 48-49 -5.106197741089659 2316 50-51 -4.652451717157245 2316 52-53 -5.992381998845648 2316 54-55 -4.938249718275525 2316 56-57 -4.3076559107541605 2316 58-59 -2.831337544348923 2316 60-61 -2.9795688627164694 2316 62-63 -4.0020092664023466 2316 64-65 -2.656794397065653 2316 66-67 -3.8769268046493046 2316 68-69 -2.7371600141497794 2316 70-71 -2.1606014405640934 2316 72-73 -1.7494989219379278 2316 74-75 -2.0582260064379945 2316 76-77 -2.768903717974407 2316 78-79 -4.640963224134435 2316 80-81 -5.595549772813687 2316 82-83 -3.099148774441936 2316 84-85 -1.8406757247990448 2316 86-87 -1.963330764771893 2316 88-89 -0.9541301264129629 2316 90-91 -0.6919262813750215 2316 92-93 -0.8967690225442624 2316 94-95 -1.400315405735352 2316 96-97 -2.7031934267499587 2316 98-99 -4.1871222461431294 2316 100-101 -3.7873264316473474 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 13740.0 3 4867.0 4 4603.0 5 5156.0 6 5550.0 7 6629.0 8 7640.0 9 9234.0 10 10739.0 11 12235.0 12 14475.0 13 15864.0 14 16819.0 15 18497.0 16 20543.0 17 21500.0 18 21173.0 19 21370.0 20 22932.0 21 24926.0 22 27871.0 23 30768.0 24 34398.0 25 38450.0 26 43905.0 27 49575.0 28 56077.0 29 63642.0 30 71964.0 31 80924.0 32 90017.0 33 99930.0 34 113367.0 35 129130.0 36 135721.0 37 103528.0 38 29033.0 39 461.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.49869281976096 19.16786471752096 12.812942067208787 23.52050039550929 2 16.218359309609607 19.60036949860828 39.58087797261021 24.60039321917191 3 19.931680239119164 23.451706807915063 29.211442204396732 27.405170748569045 4 13.096981134121807 15.798003871870373 36.4677723730661 34.63724262094172 5 13.972292635317402 37.00498841007984 34.43019614753765 14.592522807065105 6 33.4510362443168 35.25067737358687 17.14587274934703 14.152413632749292 7 29.193908202634987 31.970002102022686 20.56124005777172 18.274849637570604 8 26.800424147670643 34.874891352838524 19.557845036943426 18.76683946254741 9 26.662403962009947 16.149096119017607 19.73064950193483 37.45785041703762 10-11 25.42388280206237 26.01764925437646 27.374718329602587 21.18374961395858 12-13 26.285816946716515 23.280600121353054 27.520092743562017 22.913490188368414 14-15 22.93271773176585 24.856574326431545 25.522108774356262 26.688599167446338 16-17 22.811406296343375 27.966611584254903 26.390373289267405 22.831608830134318 18-19 22.80509998454057 26.652802903118193 28.29991022681967 22.242186885521566 20-21 23.17927721692714 26.06523572904857 28.468330774697172 22.287156279327125 22-23 22.916104227687057 25.983900791868862 28.45094469399023 22.649050286453846 24-25 22.74111385822882 26.17517308198563 28.621253735069896 22.46245932471565 26-27 22.5756609304598 26.417845122414636 28.339938114108897 22.666555833016666 28-29 22.47027590944945 26.788644710392617 28.356265001993197 22.38481437816473 30-31 22.655791237350343 26.391008637946783 28.30856972657979 22.64463039812308 32-33 22.352460253785495 26.430973975198157 28.358773511456985 22.85779225955936 34-35 22.940094216741926 26.603305998176452 28.146098363146926 22.3105014219347 36-37 23.00218261358648 26.189870420463514 28.122775915398453 22.685171050551556 38-39 22.543128112226537 26.457041819031012 28.273271169924136 22.72655889881832 40-41 22.847636518065144 26.36029616282714 28.342787984700696 22.449279334407017 42-43 22.579010544688497 26.333671267156866 28.19874036269604 22.888577825458594 44-45 22.664749770816993 26.556740218896486 28.21028333904807 22.568226671238456 46-47 23.003539621886297 26.66971952694234 27.7418840557588 22.58485679541257 48-49 22.65518929886006 26.74694535038673 27.69183627139154 22.906029079361677 50-51 22.649151217181437 26.941015498804127 27.711747762324524 22.698085521689908 52-53 22.798998093853534 26.72399019540971 27.715580007078906 22.761431703657852 54-55 22.46139906746934 26.829327885653008 27.796845413021494 22.912427633856154 56-57 22.525399739726453 27.06895756238491 27.55758111157702 22.84806158631162 58-59 22.531729394933812 26.656321741204298 27.631671348910203 23.180277514951687 60-61 22.27035166676276 26.806205125944118 27.9130956781854 23.010347529107726 62-63 22.389257274864697 26.74944527323282 27.989428737406946 22.871868714495534 64-65 22.21466340740821 26.83658852223739 27.794323518479835 23.154424551874573 66-67 22.19490911212156 26.923344332060495 27.62366106691384 23.258085488904108 68-69 22.527176604175967 26.640770943248278 27.73963775380771 23.09241469876805 70-71 22.350491518703418 26.66004366567842 27.53172918325102 23.45773563236714 72-73 22.358947355206716 26.78341985718052 27.554551416743884 23.303081370868885 74-75 22.276863903759462 27.134254543677873 27.266308415686048 23.32257313687662 76-77 22.52406395336013 26.84860197466095 27.283780734245383 23.343553337733535 78-79 22.56128328846742 26.964850527447386 27.23265473990042 23.241211444184774 80-81 22.598912815131396 27.024421103074857 27.145785062933797 23.230881018859943 82-83 22.591053188698503 27.104166334356787 27.123544949393306 23.1812355275514 84-85 22.514082179808113 27.16970069981699 26.99228660942639 23.32393051094851 86-87 22.518239989848105 27.024059516163128 27.143052483464995 23.31464801052377 88-89 22.639651506606864 26.970709371901602 26.891308906492718 23.498330214998816 90-91 22.37097961480464 26.987917806402695 27.26516054340121 23.375942035391457 92-93 22.803086202628496 26.609559410149707 27.344097155658194 23.243257231563604 94-95 22.499769038057092 26.58348187356328 27.336214017487897 23.58053507089173 96-97 22.92322345535862 26.46426272956115 26.79538338714539 23.81713042793484 98-99 22.828966020694867 26.671488111911856 26.53733649104043 23.962209376352845 100-101 23.07958242489693 26.62048074114826 26.57663807214513 23.723298761809673 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1864.0 1 1115.0 2 352.0 3 338.0 4 312.0 5 287.5 6 359.5 7 592.0 8 912.0 9 1062.0 10 992.0 11 931.5 12 928.5 13 939.0 14 1042.5 15 1150.5 16 1228.0 17 1382.5 18 1577.0 19 1821.5 20 2130.0 21 2637.0 22 3245.5 23 3927.5 24 4935.0 25 6102.5 26 7771.5 27 9880.0 28 11855.5 29 13780.5 30 15960.0 31 18847.0 32 22308.5 33 24574.5 34 26024.0 35 28211.5 36 31172.0 37 34082.5 38 36501.5 39 38701.0 40 40980.5 41 43496.0 42 45362.0 43 46812.0 44 48911.5 45 51571.5 46 53394.5 47 54751.0 48 56112.0 49 56531.0 50 56362.0 51 56056.0 52 55479.5 53 55019.0 54 53797.5 55 50641.0 56 47022.0 57 42897.5 58 38423.5 59 33707.0 60 28387.0 61 23553.5 62 19000.0 63 14500.5 64 10658.0 65 8029.5 66 6242.5 67 4880.5 68 3664.5 69 2723.0 70 2051.0 71 1465.0 72 1043.5 73 774.0 74 595.0 75 429.0 76 301.0 77 229.0 78 160.0 79 100.5 80 75.5 81 48.5 82 29.5 83 20.0 84 14.0 85 10.5 86 7.0 87 7.0 88 5.0 89 3.5 90 3.5 91 2.0 92 1.0 93 2.0 94 2.0 95 1.5 96 2.0 97 1.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.13132483061466113 2 0.11778618828325277 3 0.12442012302564286 4 0.13518334367911253 5 0.12408165696735766 6 0.1401249481300766 7 0.16808224454443482 8 0.15535592075291096 9 0.1655775957131243 10-11 0.1588421211532486 12-13 0.15014354345531877 14-15 0.15386667009645608 16-17 0.1482819801347501 18-19 0.16415603826832642 20-21 0.16984226804751792 22-23 0.20436580599260926 24-25 0.22914152145908656 26-27 0.24186784525061042 28-29 0.23628315528890445 30-31 0.22673841244526155 32-33 0.22815996989005946 34-35 0.21695674336081905 36-37 0.22555378124126335 38-39 0.2117105194573983 40-41 0.2165505840908768 42-43 0.20974741631934407 44-45 0.20270732230701174 46-47 0.21834445419978837 48-49 0.2574034373259015 50-51 0.26055117166795394 52-53 0.261397336813667 54-55 0.26014501239801174 56-57 0.23144309065542598 58-59 0.23168001689622564 60-61 0.20226731643124096 62-63 0.19485490975479489 64-65 0.1955656884771938 66-67 0.2025042426720406 68-69 0.20355348745272472 70-71 0.20967972310768704 72-73 0.24196938506809598 74-75 0.24450788050523506 76-77 0.25963731331058393 78-79 0.25645573236270297 80-81 0.2706713068106817 82-83 0.2693851357891979 84-85 0.2424770841555238 86-87 0.24616636419083257 88-89 0.25114181524762513 90-91 0.4535783647080087 92-93 0.5988479969240205 94-95 0.34855234682210834 96-97 0.2860038192510017 98-99 0.31582267898592864 100-101 0.33460754522075775 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1477253.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 71.78211610186946 #Duplication Level Percentage of deduplicated Percentage of total 1 85.30136180738994 61.23112256905638 2 9.315293023472188 13.373428906676304 3 2.525174933986855 5.43787200866925 4 1.0304032685219116 2.958581082111425 5 0.5088815356716155 1.826429673783876 6 0.3001897792869562 1.2928954553622514 7 0.20806933423741314 1.045495997522807 8 0.1309449129198954 0.7519602353732096 9 0.1072246673502215 0.6927132168646138 >10 0.5275211821189941 6.665597673642557 >50 0.028683545013588618 1.4111909537382257 >100 0.01434010304909558 1.9484707615041084 >500 0.0014339302359629123 0.6824295244875437 >1k 4.7797674532097076E-4 0.6818119412074645 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3401 0.23022461284559922 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2707 0.18324552395561222 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1719 0.1163646308384549 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 2.0307963497112546E-4 2 0.0 0.0 0.0 0.0 2.0307963497112546E-4 3 0.0 0.0 0.0 0.0 2.0307963497112546E-4 4 0.0 0.0 0.0 0.0 2.0307963497112546E-4 5 0.0 0.0 0.0 0.0 2.0307963497112546E-4 6 0.0 0.0 0.0 0.0 2.707728466281673E-4 7 0.0 0.0 0.0 0.0 2.707728466281673E-4 8 0.0 0.0 0.0 0.0 2.707728466281673E-4 9 0.0 0.0 0.0 0.0 2.707728466281673E-4 10-11 0.0 0.0 0.0 0.0 2.707728466281673E-4 12-13 0.0 0.0 0.0 0.0 5.753922990848555E-4 14-15 0.0 0.0 0.0 6.769321165704182E-5 6.430855107418973E-4 16-17 0.0 0.0 0.0 6.769321165704182E-5 6.769321165704182E-4 18-19 0.0 0.0 0.0 6.769321165704182E-5 7.4462532822746E-4 20-21 0.0 0.0 0.0 2.0307963497112546E-4 9.477049631985855E-4 22-23 0.0 0.0 0.0 3.046194524566882E-4 0.0010153981748556272 24-25 0.0 0.0 0.0 4.400058757707718E-4 0.001083091386512669 26-27 0.0 0.0 0.0 0.0014554040506263991 0.0011846312039982317 28-29 0.0 0.0 0.0 0.005990849231648201 0.0013538642331408365 30-31 0.0 0.0 0.0 0.022304913240995282 0.0013877108389693574 32-33 0.0 0.0 0.0 0.05970541268151089 0.0014215574447978782 34-35 0.0 0.0 0.0 0.11677079010839714 0.0016246370797690036 36-37 0.0 0.0 0.0 0.22003678449121444 0.0016246370797690036 38-39 0.0 0.0 0.0 0.3697403220707624 0.0016246370797690036 40-41 0.0 0.0 0.0 0.5281762839540688 0.0016246370797690036 42-43 0.0 0.0 0.0 0.6896922869677706 0.0016246370797690036 44-45 0.0 0.0 0.0 0.886205680408163 0.0017261768972545665 46-47 0.0 0.0 0.0 1.1229288415728382 0.0018277167147401291 48-49 0.0 0.0 0.0 1.3738675771854925 0.0018277167147401291 50-51 0.0 0.0 0.0 1.6399695922093236 0.0020307963497112544 52-53 0.0 0.0 0.0 1.8885729120198098 0.0020307963497112544 54-55 0.0 0.0 0.0 2.1564687971525527 0.0020984895613682963 56-57 0.0 0.0 0.0 2.467789877563288 0.0021323361671968175 58-59 0.0 0.0 0.0 2.7993850748653073 0.002166182773025338 60-61 0.0 0.0 0.0 3.152371327050952 0.002166182773025338 62-63 0.0 0.0 0.0 3.4837295981121716 0.00223387598468238 64-65 0.0 0.0 0.0 3.821687957309953 0.0022677225905109013 66-67 0.0 0.0 0.0 4.214139351891653 0.002301569196339422 68-69 0.0 0.0 0.0 4.639421954127018 0.002301569196339422 70-71 0.0 0.0 0.0 5.092120307083485 0.002301569196339422 72-73 0.0 0.0 0.0 5.531584637161001 0.002538495437139068 74-75 0.0 0.0 0.0 5.953651811842656 0.002640035254624631 76-77 0.0 0.0 0.0 6.401408560348159 0.002640035254624631 78-79 0.0 0.0 0.0 6.903522957814268 0.002707728466281673 80-81 0.0 0.0 0.0 7.418871378159327 0.002707728466281673 82-83 0.0 0.0 0.0 7.901997829755635 0.0028431148895957563 84-85 0.0 0.0 0.0 8.355982353733586 0.0028431148895957563 86-87 0.0 0.0 0.0 8.811219202127191 0.002876961495424277 88-89 3.384660582852091E-5 0.0 0.0 9.308899694229762 0.00297850131290984 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1985 0.0 42.303185 1 GTATCAA 3465 0.0 41.48582 1 TATCAAC 4475 0.0 32.652615 2 ATCAACG 4505 0.0 32.329865 3 TCAACGC 4540 0.0 31.87163 4 CAACGCA 4675 0.0 30.849794 5 TACATGG 4080 0.0 30.465017 2 AACGCAG 4860 0.0 29.67547 6 GTACATG 4310 0.0 28.398981 1 TACCGTA 85 6.4542243E-4 27.906885 7 ACATGGG 4275 0.0 27.743687 3 ACGCAGA 5360 0.0 26.376173 7 CGCAGAG 5505 0.0 25.768484 8 CGCTATA 140 1.7969205E-5 23.720852 1 TAGTACG 140 1.7969205E-5 23.720852 4 CATGGGG 2690 0.0 22.574492 4 GCAGAGT 6135 0.0 22.503675 9 AGAGTAC 5200 0.0 21.212637 10-11 GAGTACT 3770 0.0 20.260595 12-13 GTATAGC 680 0.0 20.232492 1 >>END_MODULE