FastQCFastQC Report
Fri 27 May 2016
SRR939759_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939759_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1477253
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37490.2537818505022498No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32460.21973216503875775No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22380.1514974076884596No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA26750.046.5300331
GTATCAA43200.035.687591
TCAACGC51000.029.6667774
ATCAACG51800.029.2086033
CAACGCA52450.028.8466285
TATCAAC54200.027.916182
AACGCAG54900.027.4529916
TACATGG45850.024.8786182
GTACATG48500.024.205321
ACATGGG45750.024.107953
ACGCAGA62750.023.8685157
CGCAGAG62950.023.7926838
GAGTACT42100.020.97676712-13
GCAGAGT72250.020.1433949
CATGGGG25550.020.1081054
CAGAGTA66750.019.7921810-11
TAGGACA8100.019.7846974
GAGTACA34750.019.5371361
GTACTTT45700.019.47925414-15
AGAGTAC59950.019.40527310-11
Produced by FastQC (version 0.11.5)