##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939759_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1477253 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.365338909448823 31.0 31.0 34.0 30.0 34.0 2 31.581002035534876 31.0 31.0 34.0 30.0 34.0 3 31.643423976800182 31.0 31.0 34.0 30.0 34.0 4 35.455869102990484 37.0 35.0 37.0 33.0 37.0 5 35.18163645631452 37.0 35.0 37.0 32.0 37.0 6 35.19923939907382 37.0 35.0 37.0 32.0 37.0 7 35.12080124393046 37.0 35.0 37.0 32.0 37.0 8 35.06021649642952 37.0 35.0 37.0 32.0 37.0 9 36.66390997344395 39.0 35.0 39.0 32.0 39.0 10-11 36.516569605883355 38.0 35.0 39.0 32.0 39.0 12-13 36.450981991574906 38.0 35.0 39.0 32.0 39.0 14-15 37.49403622805301 39.0 36.0 41.0 32.0 41.0 16-17 37.409324266053275 39.0 36.0 41.0 32.0 41.0 18-19 37.37238001885933 39.0 36.0 41.0 32.0 41.0 20-21 37.286838138084676 39.0 36.0 40.0 31.0 41.0 22-23 37.13602950882483 39.0 36.0 40.0 31.0 41.0 24-25 36.96434970854688 39.0 36.0 40.0 31.0 41.0 26-27 36.65880048982808 38.5 35.5 40.0 30.0 41.0 28-29 36.4284255980526 38.0 35.0 40.0 30.0 41.0 30-31 36.170300551090435 38.0 35.0 40.0 30.0 41.0 32-33 36.1352645755331 38.0 35.0 40.0 30.0 41.0 34-35 36.226725889201106 38.0 35.0 40.0 30.0 41.0 36-37 36.18992379775163 38.0 35.0 40.0 30.0 41.0 38-39 36.05208721864163 38.0 35.0 40.0 29.5 41.0 40-41 35.870249375022425 38.0 35.0 40.0 29.0 41.0 42-43 35.67323708261212 38.0 34.0 40.0 28.0 41.0 44-45 35.44172291408445 38.0 34.0 40.0 27.0 41.0 46-47 35.15220920180903 38.0 34.0 40.0 26.5 41.0 48-49 34.84366658927076 38.0 33.0 40.0 26.0 41.0 50-51 34.56650722658881 38.0 33.0 40.0 24.5 41.0 52-53 34.27624381199429 37.5 33.0 40.0 24.0 41.0 54-55 33.94175168369941 37.0 33.0 40.0 23.5 41.0 56-57 32.61621029031588 36.5 31.0 39.5 16.0 40.5 58-59 29.115493080738368 34.0 23.5 38.5 7.0 40.0 60-61 29.134532811915086 34.0 24.0 38.0 8.0 40.0 62-63 30.96583286681428 34.5 29.0 38.0 8.0 40.0 64-65 30.415802167942797 35.0 29.0 38.0 2.0 40.0 66-67 24.807668693175778 28.0 17.5 36.0 2.0 39.0 68-69 17.055327015751534 13.0 7.0 28.5 2.0 35.0 70-71 15.931759488726712 12.5 7.5 25.5 2.0 33.0 72-73 17.932350788930535 16.5 7.0 29.0 2.0 34.0 74-75 18.677775235521608 17.5 7.5 30.0 2.0 35.0 76-77 19.788748102051578 22.5 7.5 30.5 2.0 34.5 78-79 22.906025575849227 27.5 11.0 33.5 2.0 35.5 80-81 25.564880220246636 31.5 21.0 34.5 2.0 36.0 82-83 26.537566347809076 32.5 25.0 35.0 2.0 36.0 84-85 26.52964454971491 33.0 25.0 35.0 2.0 36.0 86-87 26.237356431159725 33.0 24.5 35.0 2.0 35.5 88-89 25.772176465371878 32.5 23.0 35.0 2.0 35.0 90-91 24.098097617672803 31.5 10.0 34.0 2.0 35.0 92-93 21.924065816755828 30.5 2.0 34.0 2.0 35.0 94-95 17.389799513015035 19.5 2.0 33.0 2.0 35.0 96-97 16.02992446114511 10.5 2.0 31.5 2.0 34.5 98-99 14.895642114113155 2.0 2.0 31.0 2.0 34.0 100-101 14.252178536784152 2.0 2.0 31.5 2.0 34.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.3602554091433916 1101 2 0.31587258375849814 1101 3 0.38039630242564115 1101 4 0.38075733133978673 1101 5 0.4477324687562785 1101 6 0.3869254474348409 1101 7 0.3671906692219835 1101 8 0.3473205807841637 1101 9 0.5666913989822646 1101 10-11 0.3130394972902053 1101 12-13 0.546135619481646 1101 14-15 0.5139052762164837 1101 16-17 0.46234056887317365 1101 18-19 0.5638324755728519 1101 20-21 0.5305785839060206 1101 22-23 0.5094780443553404 1101 24-25 0.45529001336705477 1101 26-27 0.5144194048742676 1101 28-29 0.2537381082604142 1101 30-31 0.794728233119784 1101 32-33 0.5784980332086889 1101 34-35 0.7636772839669064 1101 36-37 0.7238312607911013 1101 38-39 0.779808625359891 1101 40-41 0.7399203709306619 1101 42-43 0.7434010608713919 1101 44-45 0.47319944478969944 1101 46-47 -0.00666293954235897 1101 48-49 0.10309362535999611 1101 50-51 0.346303166242933 1101 52-53 0.39584811660992614 1101 54-55 0.20341843552855465 1101 56-57 0.6068820231078575 1101 58-59 -8.540288820657572 1101 60-61 -9.628431957253863 1101 62-63 -2.689839809306637 1101 64-65 -0.03284566207408446 1101 66-67 -1.34538226496608 1101 68-69 -3.8933061134771876 1101 70-71 -4.710863718879896 1101 72-73 -7.132606510638771 1101 74-75 -7.732331498189094 1101 76-77 -9.031743942056 1101 78-79 -11.806651747298 1101 80-81 -7.963455411017012 1101 82-83 -4.482427405611549 1101 84-85 -3.4932637519029086 1101 86-87 -3.2023484384849397 1101 88-89 -3.279002069985715 1101 90-91 -8.910565135826044 1101 92-93 -13.808691437689482 1101 94-95 -12.263961661258437 1101 96-97 -12.12670730971762 1101 98-99 -11.59933538156004 1101 100-101 -11.334209318261797 1102 1 0.5159288514563087 1102 2 0.5242042999223173 1102 3 0.4591415788204891 1102 4 0.365901130053345 1102 5 0.553130168964806 1102 6 0.5637664626174228 1102 7 0.5222404721625296 1102 8 0.5713359009660905 1102 9 0.498934348417194 1102 10-11 0.7542939033420595 1102 12-13 0.5909565017858185 1102 14-15 0.7104875207536807 1102 16-17 0.6796219951813995 1102 18-19 0.7214266742271391 1102 20-21 0.7374327951918644 1102 22-23 0.9351873349060114 1102 24-25 0.8668487766028434 1102 26-27 1.0105845078850209 1102 28-29 0.9549297269706543 1102 30-31 0.795872748092421 1102 32-33 0.6803880927322936 1102 34-35 0.5016658308783519 1102 36-37 0.8279397828628134 1102 38-39 0.622348504760204 1102 40-41 0.5684517399028834 1102 42-43 0.8065557940031383 1102 44-45 0.6973334064080916 1102 46-47 0.7189943957621523 1102 48-49 0.7327638639130853 1102 50-51 0.5208630903124174 1102 52-53 0.43238085811802307 1102 54-55 0.2504820379236179 1102 56-57 1.106843652085658 1102 58-59 -8.922813510425652 1102 60-61 -10.266130560372236 1102 62-63 -3.1573823349843515 1102 64-65 -0.2658455350527653 1102 66-67 -1.4671061735530735 1102 68-69 -3.8839493176604236 1102 70-71 -4.4689273671437615 1102 72-73 -6.856213863055881 1102 74-75 -7.857611121500327 1102 76-77 -8.816508088172851 1102 78-79 -11.808842423933488 1102 80-81 -8.123300709640596 1102 82-83 -4.171924683474202 1102 84-85 -3.359374906138541 1102 86-87 -3.1447482416018993 1102 88-89 -3.257717178818286 1102 90-91 -8.708849995238147 1102 92-93 -14.337635096679593 1102 94-95 -12.338161515183923 1102 96-97 -11.934463195920635 1102 98-99 -11.457483446072754 1102 100-101 -11.161803905036761 1103 1 0.10937651486227651 1103 2 0.17409125767277445 1103 3 0.188408521295937 1103 4 0.12061107473061128 1103 5 0.13043532759062515 1103 6 0.09915700391450599 1103 7 0.14321937869340218 1103 8 0.19171203474228804 1103 9 0.22801840212657254 1103 10-11 0.05629557393480411 1103 12-13 0.2503684316781545 1103 14-15 0.3041815697589314 1103 16-17 0.34610756172518364 1103 18-19 0.3348917143016834 1103 20-21 0.25438108372862445 1103 22-23 0.29657598381860595 1103 24-25 0.2860696257433162 1103 26-27 0.22237528850256894 1103 28-29 0.21379312471303535 1103 30-31 0.2513827081520148 1103 32-33 0.22544915701075752 1103 34-35 0.1545259607696181 1103 36-37 -0.061752579451905376 1103 38-39 -0.1660941325583991 1103 40-41 -0.15833323656751475 1103 42-43 -0.06354824242794876 1103 44-45 -0.2936654703239867 1103 46-47 -0.18085924850890933 1103 48-49 -0.09785475622024364 1103 50-51 -0.09549306789665479 1103 52-53 -0.3417099298329589 1103 54-55 -0.5009365797056091 1103 56-57 0.2483049825142345 1103 58-59 -7.252717120444199 1103 60-61 -9.113436103556342 1103 62-63 -3.281439049282586 1103 64-65 -1.0140521966754612 1103 66-67 -2.6650457013154956 1103 68-69 -4.777372342506467 1103 70-71 -5.222842483503944 1103 72-73 -7.813332260503131 1103 74-75 -8.570067724544955 1103 76-77 -9.524302816325918 1103 78-79 -12.597434412958375 1103 80-81 -9.20683906619946 1103 82-83 -5.816219448294454 1103 84-85 -4.978242095804475 1103 86-87 -4.628643521909115 1103 88-89 -4.769738649691128 1103 90-91 -9.700995168673934 1103 92-93 -14.704545063536035 1103 94-95 -12.74123404645388 1103 96-97 -12.462586479941077 1103 98-99 -11.776193279854144 1103 100-101 -11.401056171694842 1104 1 0.2180220855595536 1104 2 0.2388690449044013 1104 3 0.24523502146951515 1104 4 0.26433845055816363 1104 5 0.12605580118547977 1104 6 0.24623032499134467 1104 7 0.2572985203507443 1104 8 0.2736363553484651 1104 9 0.3848394812944349 1104 10-11 0.4145798938373275 1104 12-13 0.3162230604092713 1104 14-15 0.3462512666600901 1104 16-17 0.46003609994561145 1104 18-19 0.4860056591747295 1104 20-21 0.36944139151969324 1104 22-23 0.3608359034512958 1104 24-25 0.5310931604472628 1104 26-27 0.5207248256168882 1104 28-29 0.45333139144877066 1104 30-31 0.5892169122568305 1104 32-33 0.4058894862776157 1104 34-35 0.3878519703601313 1104 36-37 0.30822800903955283 1104 38-39 0.31104466062519975 1104 40-41 0.4452804920038034 1104 42-43 0.3420962810459045 1104 44-45 0.02692742255801761 1104 46-47 -0.043355977057608186 1104 48-49 0.08597370161851359 1104 50-51 -0.02021468489290612 1104 52-53 -0.18488903016859837 1104 54-55 -0.14451317259274532 1104 56-57 0.5569652006971495 1104 58-59 -6.559911154035049 1104 60-61 -8.71574753423155 1104 62-63 -2.6047837308272186 1104 64-65 -0.3598380468453257 1104 66-67 -1.8951262876170105 1104 68-69 -4.346785089624447 1104 70-71 -5.248557436971252 1104 72-73 -8.092908244514394 1104 74-75 -8.781336164490803 1104 76-77 -9.417195052117407 1104 78-79 -12.3016986263148 1104 80-81 -8.570087734911748 1104 82-83 -4.994903335808942 1104 84-85 -4.037175428563398 1104 86-87 -3.716452864779111 1104 88-89 -3.7193624283044358 1104 90-91 -9.376253052377924 1104 92-93 -14.824686038405185 1104 94-95 -12.309806358182529 1104 96-97 -12.101800419752117 1104 98-99 -11.416351767084803 1104 100-101 -10.987799622778882 1105 1 0.1312203929277942 1105 2 0.09422702686514484 1105 3 0.0640945386108065 1105 4 0.08299527475512036 1105 5 0.08531077350977512 1105 6 0.08666810845451067 1105 7 0.037878205523234953 1105 8 0.1778191968704732 1105 9 0.1537699409662423 1105 10-11 0.15128030242800605 1105 12-13 0.22227821037083828 1105 14-15 0.2247691025986711 1105 16-17 0.1643741035408084 1105 18-19 0.19917433258478212 1105 20-21 0.04493082006293747 1105 22-23 0.10798519575325116 1105 24-25 0.16112380578913843 1105 26-27 0.16577909696489002 1105 28-29 0.015885264278296063 1105 30-31 0.013472598376608858 1105 32-33 0.19397734263949928 1105 34-35 0.20784544423001705 1105 36-37 0.03555667835412635 1105 38-39 0.29699267050929734 1105 40-41 0.18022096811995425 1105 42-43 0.31480286448775985 1105 44-45 0.38876616804269304 1105 46-47 0.15708965607481673 1105 48-49 0.07902366438877095 1105 50-51 0.14708316011066813 1105 52-53 0.11283506519448139 1105 54-55 -0.06596653511053319 1105 56-57 0.6897153443927166 1105 58-59 -6.940223675755867 1105 60-61 -8.731813343261503 1105 62-63 -2.910292150908557 1105 64-65 -0.6120615301755983 1105 66-67 -2.062613506932024 1105 68-69 -4.161927459566957 1105 70-71 -4.745973414086512 1105 72-73 -6.372415363531779 1105 74-75 -7.0625172691798355 1105 76-77 -8.834510273774836 1105 78-79 -11.963793880344829 1105 80-81 -8.525638878670158 1105 82-83 -4.787276878511186 1105 84-85 -3.8775297624673506 1105 86-87 -3.533938065488826 1105 88-89 -3.739781349253768 1105 90-91 -8.916130704086518 1105 92-93 -11.787165368511246 1105 94-95 -11.768686743956732 1105 96-97 -11.905536629244224 1105 98-99 -11.168144683986107 1105 100-101 -10.779797122205556 1106 1 0.11090097658029308 1106 2 0.17182209492708722 1106 3 0.15086426903194905 1106 4 0.08522784938526229 1106 5 0.041225382304816094 1106 6 0.0993960854696212 1106 7 0.01171903210962455 1106 8 0.07132602363218155 1106 9 0.08806740962131698 1106 10-11 0.215868994993329 1106 12-13 0.18876290188229206 1106 14-15 0.21660886847950422 1106 16-17 0.1940278267593385 1106 18-19 0.20359834644936115 1106 20-21 0.05742202369930283 1106 22-23 0.26178138064118883 1106 24-25 0.2673055253172336 1106 26-27 0.20634520815680446 1106 28-29 0.2568603298623202 1106 30-31 0.46813527099223506 1106 32-33 0.4973286041494447 1106 34-35 0.43507908397307204 1106 36-37 0.306299324055459 1106 38-39 0.2621847801527508 1106 40-41 0.3968717927622123 1106 42-43 0.4580390056546477 1106 44-45 0.07746598102355051 1106 46-47 0.13759294764103203 1106 48-49 0.31523804248279674 1106 50-51 0.1675399392973418 1106 52-53 0.33144594247045234 1106 54-55 -0.056258826582229915 1106 56-57 0.9096996052067894 1106 58-59 -6.664956871089739 1106 60-61 -8.81570775849492 1106 62-63 -2.6819363473653297 1106 64-65 -0.4226271565574926 1106 66-67 -1.5795926312084205 1106 68-69 -3.943938265443638 1106 70-71 -4.623238615451617 1106 72-73 -5.938466321472305 1106 74-75 -6.704791870472565 1106 76-77 -8.435847076193205 1106 78-79 -11.575127808182572 1106 80-81 -7.914774335079617 1106 82-83 -4.15061407563347 1106 84-85 -3.1462805245740135 1106 86-87 -2.9500101053196417 1106 88-89 -3.1809141494919615 1106 90-91 -8.263859467081506 1106 92-93 -11.571949630500624 1106 94-95 -11.553538340671189 1106 96-97 -11.963016488815818 1106 98-99 -11.465833952104166 1106 100-101 -11.030173094825054 1107 1 0.014240453703564526 1107 2 0.07505989548351621 1107 3 0.06454086378404256 1107 4 -0.03104329211362966 1107 5 0.02116668900819718 1107 6 -0.09521834602379897 1107 7 0.001742268195634722 1107 8 -0.20236287199084302 1107 9 -0.16325313971125865 1107 10-11 -0.1309562538899769 1107 12-13 -0.013703053912315966 1107 14-15 0.031202894881523946 1107 16-17 0.018196396538868953 1107 18-19 -0.03734296922547031 1107 20-21 0.01525864540565891 1107 22-23 -0.080105327739453 1107 24-25 0.09658483782415317 1107 26-27 0.16448038017461641 1107 28-29 0.2564232203078092 1107 30-31 0.11448448414519419 1107 32-33 -0.005425883824301536 1107 34-35 -0.1257142823287154 1107 36-37 -0.07837577445617683 1107 38-39 -0.051295289744906825 1107 40-41 -0.08613426496814469 1107 42-43 -0.067585233640159 1107 44-45 0.09926862680731574 1107 46-47 0.12214209679756749 1107 48-49 0.13546888816269842 1107 50-51 0.0377829462920829 1107 52-53 0.044990656734384515 1107 54-55 0.07873874613893861 1107 56-57 0.6377840685289655 1107 58-59 -6.8973461408227195 1107 60-61 -8.489713994657684 1107 62-63 -3.161075705068477 1107 64-65 -1.0743532658039783 1107 66-67 -2.4263635529064977 1107 68-69 -4.376229646560306 1107 70-71 -5.030545970754401 1107 72-73 -6.626481495204663 1107 74-75 -7.08308028502587 1107 76-77 -9.203765328233466 1107 78-79 -12.146908723092244 1107 80-81 -8.620483723515004 1107 82-83 -4.887215446672197 1107 84-85 -3.9686896910155625 1107 86-87 -3.7728907719191938 1107 88-89 -3.704026130281072 1107 90-91 -9.035658374756196 1107 92-93 -12.268971653445636 1107 94-95 -12.165482745519366 1107 96-97 -12.192982217831819 1107 98-99 -11.516040245883655 1107 100-101 -11.11606362704207 1108 1 0.023139479031694776 1108 2 0.03587557658232399 1108 3 -0.08456015125417693 1108 4 -0.06002106692731246 1108 5 -0.21992178087017322 1108 6 0.0544447265750847 1108 7 -0.021878628586726734 1108 8 -0.030772095526529597 1108 9 -0.04365027440236702 1108 10-11 0.037317900325277265 1108 12-13 -0.0985105634576442 1108 14-15 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3.2778871756809878 2312 92-93 5.2064205980808005 2312 94-95 9.072477974891992 2312 96-97 9.89431795297228 2312 98-99 10.044891930431866 2312 100-101 9.22470438458046 2313 1 -0.36038198127284105 2313 2 -0.31640292488690136 2313 3 -0.3557423055048261 2313 4 -0.49685103940752384 2313 5 -0.5127926647509753 2313 6 -0.4754482710303378 2313 7 -0.29293491594859944 2313 8 -0.45605611183017913 2313 9 -0.6140308430410002 2313 10-11 -0.553834007024264 2313 12-13 -0.5648382571256079 2313 14-15 -0.6386265693434083 2313 16-17 -0.5647805715468053 2313 18-19 -0.5623419838837762 2313 20-21 -0.7113690495038938 2313 22-23 -0.6844964582309387 2313 24-25 -0.6244313361119467 2313 26-27 -0.6925160833444508 2313 28-29 -0.6752061586402647 2313 30-31 -0.9223225071228853 2313 32-33 -0.8039781481395352 2313 34-35 -0.7195313527466141 2313 36-37 -0.7877134098510865 2313 38-39 -1.008214359152376 2313 40-41 -1.2119758363552577 2313 42-43 -0.9301564917602718 2313 44-45 -1.0507989138872915 2313 46-47 -0.7293991567720539 2313 48-49 -1.118667307558411 2313 50-51 -0.7324219331200084 2313 52-53 -0.9326407430599488 2313 54-55 -0.623964852328946 2313 56-57 0.4716653115643865 2313 58-59 3.8690490492765655 2313 60-61 3.6915369235016264 2313 62-63 0.9650847587229023 2313 64-65 1.0389637168289276 2313 66-67 1.8640215723752185 2313 68-69 0.13182552182563256 2313 70-71 2.685641580976041 2313 72-73 8.403480506436377 2313 74-75 10.000936666434406 2313 76-77 8.801336054469669 2313 78-79 6.4535773190947125 2313 80-81 3.556621593388801 2313 82-83 1.9664545928489616 2313 84-85 1.4804129314265815 2313 86-87 1.6368528242555342 2313 88-89 1.7391940022205148 2313 90-91 3.071873756340832 2313 92-93 5.207290121387661 2313 94-95 8.93807756276718 2313 96-97 9.412430980730658 2313 98-99 9.722363178089171 2313 100-101 9.021050253376862 2314 1 -0.40786779931174166 2314 2 -0.47949628953179513 2314 3 -0.4185696518012776 2314 4 -0.2861707600070602 2314 5 -0.35034648149713377 2314 6 -0.4348565182487576 2314 7 -0.49089406150675785 2314 8 -0.6577883167779888 2314 9 -0.5904432331947476 2314 10-11 -0.5299201674115608 2314 12-13 -0.4599975108450778 2314 14-15 -0.7450312696505179 2314 16-17 -0.6041467803955669 2314 18-19 -0.7301564433939305 2314 20-21 -0.632856689719759 2314 22-23 -0.47261743161774206 2314 24-25 -0.4614676919528975 2314 26-27 -0.6847271700407731 2314 28-29 -0.726953264188495 2314 30-31 -0.6526196096666226 2314 32-33 -0.7118688889795166 2314 34-35 -0.897165592133895 2314 36-37 -0.7242709570254959 2314 38-39 -0.4898378993401451 2314 40-41 -0.6310761324184142 2314 42-43 -0.6284203485066229 2314 44-45 -0.5669004945689764 2314 46-47 -0.4330753419585065 2314 48-49 -0.5046884737191917 2314 50-51 -0.4149766187295185 2314 52-53 -0.5517243520746931 2314 54-55 -0.49348878017593023 2314 56-57 0.9891826522903671 2314 58-59 4.153153559732967 2314 60-61 3.694053785134731 2314 62-63 1.2886685520666 2314 64-65 1.366219878856512 2314 66-67 1.9008786337981718 2314 68-69 -0.060133391494286315 2314 70-71 2.7042027757524654 2314 72-73 8.57512912997819 2314 74-75 10.137209153348675 2314 76-77 8.835205078598083 2314 78-79 6.632083263906463 2314 80-81 3.6158110819287366 2314 82-83 2.30941261781701 2314 84-85 1.8124896609924583 2314 86-87 1.7278569217176418 2314 88-89 1.922949262091855 2314 90-91 3.321953334479254 2314 92-93 5.572353816379273 2314 94-95 9.281278991275965 2314 96-97 10.229352338041373 2314 98-99 10.432485598498854 2314 100-101 9.461076757769087 2315 1 -0.719584938217448 2315 2 -0.6350029560015926 2315 3 -0.6840324657454957 2315 4 -0.6022682297507842 2315 5 -0.8915978780351992 2315 6 -0.8209703773202293 2315 7 -0.7620365403802793 2315 8 -0.8982291060250134 2315 9 -1.1377680322655266 2315 10-11 -1.0101840700402533 2315 12-13 -0.8437760137562691 2315 14-15 -1.0237655016899296 2315 16-17 -0.9964792327410166 2315 18-19 -0.8915839390751188 2315 20-21 -0.9198938634712377 2315 22-23 -1.0356080655844977 2315 24-25 -0.8730908260057859 2315 26-27 -0.9955460950386836 2315 28-29 -1.1303177520096952 2315 30-31 -1.224562852391088 2315 32-33 -0.9978725015220249 2315 34-35 -1.207772883169966 2315 36-37 -1.2067225642131731 2315 38-39 -1.2538906404921235 2315 40-41 -1.0112445536782673 2315 42-43 -1.2721124260138765 2315 44-45 -1.1325291704786338 2315 46-47 -1.068757320096232 2315 48-49 -1.2592447560487372 2315 50-51 -1.3083155628236938 2315 52-53 -1.1405613988458043 2315 54-55 -1.1654684436287184 2315 56-57 -0.04400696681771876 2315 58-59 3.417851046210547 2315 60-61 2.9059682850141506 2315 62-63 0.37967361651652354 2315 64-65 0.2451160354853279 2315 66-67 1.300379571385843 2315 68-69 5.219282498771044 2315 70-71 4.1375405386338375 2315 72-73 8.013696220404952 2315 74-75 9.207825992521627 2315 76-77 8.053254502510754 2315 78-79 5.390601530870402 2315 80-81 2.415541371670397 2315 82-83 0.9377095550306258 2315 84-85 0.3329271034698529 2315 86-87 -0.01514070109157828 2315 88-89 0.15368934113467958 2315 90-91 1.5592491446189811 2315 92-93 3.4258588193076953 2315 94-95 7.405121771850919 2315 96-97 8.224275583817306 2315 98-99 8.316072154328385 2315 100-101 7.494468167398923 2316 1 -0.46276982370393327 2316 2 -0.39284495077286863 2316 3 -0.5683303390970913 2316 4 -0.4145588831763902 2316 5 -0.656827591270094 2316 6 -0.5136114163533136 2316 7 -0.7985997818981474 2316 8 -0.8368558555404277 2316 9 -0.8022049018010549 2316 10-11 -0.6761131287390043 2316 12-13 -0.5006428238709546 2316 14-15 -0.6938192971217987 2316 16-17 -1.0197628636150284 2316 18-19 -0.9612030259057889 2316 20-21 -0.7950412274562808 2316 22-23 -0.8140972640650546 2316 24-25 -2.0638936645113475 2316 26-27 -1.5110203439854928 2316 28-29 -1.128070011752449 2316 30-31 -0.6734596168574924 2316 32-33 -0.7288039291983779 2316 34-35 -0.6139574907804715 2316 36-37 -0.6499779128408392 2316 38-39 -1.0607990835088827 2316 40-41 -0.764922288344053 2316 42-43 -0.7900189384472469 2316 44-45 -0.7069710786379488 2316 46-47 -0.7695350818706785 2316 48-49 -1.1316681595106033 2316 50-51 -0.9056771473666174 2316 52-53 -0.7267875763149547 2316 54-55 -0.7588386525109527 2316 56-57 0.1303163227295343 2316 58-59 3.654814758905207 2316 60-61 3.641352275187135 2316 62-63 1.344075424213738 2316 64-65 1.1391613908586429 2316 66-67 6.348986772592784 2316 68-69 8.990413560698592 2316 70-71 5.864089478995249 2316 72-73 9.554558780423498 2316 74-75 10.775707748290465 2316 76-77 9.223141717839422 2316 78-79 6.41496223426034 2316 80-81 3.385961400854651 2316 82-83 2.0949732961918173 2316 84-85 1.8537770160092393 2316 86-87 1.692826434860578 2316 88-89 1.6019377283143648 2316 90-91 3.1932333769756767 2316 92-93 5.1212902569255085 2316 94-95 8.93292052368827 2316 96-97 9.499774342104484 2316 98-99 9.731575791206069 2316 100-101 8.844242011267538 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 10.0 4 24.0 5 59.0 6 164.0 7 386.0 8 930.0 9 1619.0 10 2559.0 11 3875.0 12 5368.0 13 7476.0 14 9718.0 15 13397.0 16 17645.0 17 22861.0 18 25604.0 19 27467.0 20 29079.0 21 28874.0 22 27791.0 23 26744.0 24 27090.0 25 28463.0 26 37347.0 27 54247.0 28 75058.0 29 98791.0 30 115731.0 31 122188.0 32 125440.0 33 127359.0 34 127416.0 35 120328.0 36 95999.0 37 54241.0 38 15334.0 39 569.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.281911457832585 19.4298234329488 14.062531737788653 24.225733371429964 2 16.302121201043608 20.586088917953237 38.47068269177393 24.64110718922922 3 19.696048632218847 23.79174254168331 29.815801409413695 26.696407416684153 4 13.561996489430042 16.618412688957136 36.03600737314461 33.7835834484682 5 13.67582619674825 37.33713650154134 34.645295408223056 14.341741893487356 6 33.35716137609654 35.99862176434472 16.647001762056686 13.99721509750205 7 28.847462147648372 32.089560826750734 20.530944936310842 18.532032089290055 8 26.63053190798024 35.43975012997141 19.28003964127892 18.649678320769432 9 26.104939370574982 16.038146478632974 19.724786478687133 38.132127672104914 10-11 25.276408306498617 26.22993488589971 27.353236040136657 21.140420767465017 12-13 25.882702556704913 23.386041524369894 27.698708345828372 23.032547573096824 14-15 22.414531310342216 25.067769435333116 25.57353977978699 26.94415947453768 16-17 22.47719246466245 28.170462337866297 26.485172140452583 22.867173057018668 18-19 22.447967631298997 26.817329331698293 28.357408242930433 22.377294794072284 20-21 22.92799540769252 26.151207680742566 28.560510623434173 22.36028628813074 22-23 22.591797071997824 26.172192576356252 28.52798403523296 22.708026316412962 24-25 22.590375514553024 26.349074938416102 28.555975178253153 22.504574368777725 26-27 22.355314823044566 26.615865081785383 28.33903254728971 22.689787547880336 28-29 22.34176754607903 26.892180738449145 28.31587088563465 22.450180829837176 30-31 22.524652936214462 26.54894096970698 28.25775536759041 22.668650726488146 32-33 22.224971331789018 26.45541094712729 28.390362877949915 22.92925484313378 34-35 22.780972560222306 26.62088293331799 28.215212880821266 22.382931625638438 36-37 22.893091428138067 26.231961387618902 28.187701783276637 22.687245400966393 38-39 22.388936418873875 26.502636870275193 28.336173282851863 22.772253427999072 40-41 22.673017289625175 26.454308118746443 28.3689748955604 22.50369969606798 42-43 22.433309328720654 26.404125949392586 28.178774437559056 22.983790284327696 44-45 22.520003670118633 26.570677038701806 28.245795022520102 22.663524268659454 46-47 22.802234078500177 26.659628741549916 27.85867178236695 22.679465397582955 48-49 22.522355369368498 26.812121450150855 27.748218155766246 22.917305024714402 50-51 22.510279497375322 26.92728937821948 27.757740728138824 22.804690396266373 52-53 22.700127563802212 26.72481969086768 27.71668919120216 22.858363554127948 54-55 22.419149976235516 26.78954687696768 27.82976775802745 22.961535388769352 56-57 22.546179043445722 26.808724395442297 27.740372360906985 22.904724200205 58-59 21.08953830913926 26.380931459298967 29.524773739140635 23.00475649242113 60-61 22.299920928165495 26.582565560192883 28.181113544641974 22.936399966999645 62-63 22.13425450661078 26.760259712918593 28.005447973645563 23.100037806825064 64-65 21.470661915830743 26.776504040594766 28.46440449406208 23.28842954951241 66-67 21.968614302676105 26.124432052551516 29.022067392251838 22.884886252520538 68-69 22.89058596816787 25.09818940170702 29.70996655937582 22.301258070749288 70-71 21.957691924199704 25.47894497775956 29.649789228204092 22.913573869836647 72-73 21.722312787887237 25.684821363251753 29.619861165366157 22.973004683494853 74-75 22.217907371584083 25.940985226012565 28.76713977914691 23.073967623256443 76-77 20.60231972174294 26.43726207104319 29.30267986744447 23.657738339769395 78-79 21.560871967025026 26.702417881273107 28.07057323758127 23.666136914120596 80-81 22.180335082078187 26.74642071416483 27.24061600947707 23.832628194279913 82-83 22.33994843799342 26.831840639079154 27.017286506249278 23.810924416678148 84-85 22.171463578501303 26.832007392101215 26.98655104662202 24.009977982775457 86-87 22.307473338999444 26.728560602137346 26.969522500100695 23.994443558762512 88-89 22.211301047045108 26.74011382617393 26.77978206163131 24.268803065149662 90-91 21.94728585812804 26.708365109822257 27.127780838573628 24.21656819347607 92-93 21.827041458660183 26.505996317001028 27.327126735242828 24.339835489095957 94-95 20.673412870340545 26.032915957862468 28.9093317224225 24.384339449374487 96-97 20.42017034414896 25.907326280076294 29.065583491989887 24.60691988378486 98-99 20.731120023695954 26.102590342531016 28.25130160146061 24.914988032312415 100-101 20.558161003301695 26.20570974866474 28.193116134387175 25.04301311364639 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 189.0 1 146.0 2 101.0 3 124.5 4 159.5 5 188.0 6 231.0 7 342.0 8 461.0 9 588.0 10 695.0 11 759.5 12 860.0 13 964.0 14 1070.5 15 1173.0 16 1316.0 17 1451.0 18 1610.0 19 1884.5 20 2224.0 21 2660.5 22 3253.0 23 3990.5 24 4886.5 25 6195.5 26 7926.5 27 9732.0 28 11616.5 29 13703.0 30 16106.0 31 18815.5 32 21800.0 33 24717.5 34 27325.5 35 29968.0 36 32586.0 37 35453.5 38 38223.5 39 40504.5 40 43028.5 41 45464.5 42 47595.5 43 49947.0 44 52107.5 45 54050.0 46 55605.5 47 56966.5 48 58136.5 49 58636.5 50 58713.0 51 58061.5 52 57094.5 53 55927.0 54 53948.0 55 50569.0 56 46125.0 57 41096.5 58 35472.5 59 29979.0 60 24680.0 61 19825.5 62 15524.5 63 11577.5 64 8444.0 65 6252.0 66 4718.0 67 3503.5 68 2504.5 69 1759.0 70 1239.5 71 852.5 72 586.5 73 432.5 74 319.0 75 226.0 76 150.0 77 86.5 78 57.5 79 32.5 80 19.5 81 15.0 82 7.0 83 3.0 84 3.5 85 2.0 86 0.5 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02078181597871184 2 0.004806218027649969 3 0.0029108081012527982 4 0.0 5 0.06363161895761932 6 1.3538642331408364E-4 7 0.0 8 3.3846605828520906E-4 9 0.0 10-11 0.0 12-13 0.0 14-15 1.0153981748556274E-4 16-17 0.0 18-19 0.001590790473940483 20-21 0.0 22-23 0.0 24-25 0.0 26-27 1.6923302914260453E-4 28-29 0.0024031090138249846 30-31 0.009950902113585148 32-33 4.738524815992927E-4 34-35 0.001218477809826753 36-37 0.028498842107614608 38-39 0.027584983750244544 40-41 0.029954246158241007 42-43 0.030698871486468465 44-45 0.0312404171797248 46-47 0.033305060135264575 48-49 0.03266197462452268 50-51 0.02744959732693046 52-53 0.023354158021679425 54-55 0.01753254181917383 56-57 0.14384807477121386 58-59 0.7762380580712985 60-61 0.3074287207404554 62-63 4.4000587577077186E-4 64-65 0.15464514203051205 66-67 1.45286555518926 68-69 2.7839171760016734 70-71 2.236380633513691 72-73 1.91808715230228 74-75 1.5987105797043568 76-77 1.1327105106572808 78-79 0.4359781296771778 80-81 2.0307963497112548E-4 82-83 1.6923302914260453E-4 84-85 3.384660582852091E-5 86-87 0.0032831207653665284 88-89 3.384660582852091E-5 90-91 0.2313077042321119 92-93 0.17867623216876188 94-95 1.333048570556296 96-97 1.3159221880070646 98-99 1.042475459518444 100-101 0.4190209801570889 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1477253.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 66.00402700674053 #Duplication Level Percentage of deduplicated Percentage of total 1 81.91061910956063 54.06430715846275 2 11.18694470129494 14.767668003743681 3 3.1881202711191827 6.312863294270625 4 1.2726915568193335 3.3601107155021577 5 0.6877086182440688 2.269576910567486 6 0.39345685669007 1.558184219697517 7 0.2603258959520863 1.2027790226882797 8 0.17676497992199267 0.933376040689372 9 0.13855027638828418 0.8230388566071306 >10 0.72216215421209 8.578532150963996 >50 0.03915143174416486 1.7701189475655312 >100 0.020938294072109252 2.597118906293522 >500 0.0018474148663405957 0.8286284395426012 >1k 7.184391146880095E-4 0.9336973334054671 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3749 0.2537818505022498 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3246 0.21973216503875775 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2238 0.1514974076884596 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 6.769321165704182E-5 2 0.0 0.0 0.0 0.0 6.769321165704182E-5 3 0.0 0.0 0.0 0.0 6.769321165704182E-5 4 0.0 0.0 0.0 0.0 6.769321165704182E-5 5 0.0 0.0 0.0 0.0 6.769321165704182E-5 6 0.0 0.0 0.0 0.0 2.0307963497112546E-4 7 0.0 0.0 0.0 6.769321165704182E-5 2.0307963497112546E-4 8 0.0 0.0 0.0 6.769321165704182E-5 2.0307963497112546E-4 9 0.0 0.0 0.0 6.769321165704182E-5 2.0307963497112546E-4 10-11 0.0 0.0 0.0 1.0153981748556273E-4 2.0307963497112546E-4 12-13 0.0 0.0 0.0 1.3538642331408364E-4 7.4462532822746E-4 14-15 0.0 0.0 0.0 1.3538642331408364E-4 8.123185398845018E-4 16-17 0.0 0.0 0.0 1.3538642331408364E-4 8.123185398845018E-4 18-19 0.0 0.0 0.0 1.3538642331408364E-4 9.138583573700646E-4 20-21 0.0 0.0 0.0 3.7231266411373E-4 0.001150784598169711 22-23 0.0 0.0 0.0 5.753922990848555E-4 0.001150784598169711 24-25 0.0 0.0 0.0 7.107787223989391E-4 0.001150784598169711 26-27 0.0 0.0 0.0 0.0017261768972545665 0.0011846312039982317 28-29 0.0 0.0 0.0 0.006633934742390098 0.0012184778098267527 30-31 0.0 0.0 0.0 0.02348954444499351 0.0012184778098267527 32-33 0.0 0.0 0.0 0.06241314114779256 0.0012861710214837946 34-35 0.0 0.0 0.0 0.12184778098267528 0.0012861710214837946 36-37 0.0 0.0 0.0 0.22914152145908656 0.0012861710214837946 38-39 0.0 0.0 0.0 0.3857836132334813 0.0013538642331408365 40-41 0.0 0.0 0.0 0.5516996750048908 0.0013538642331408365 42-43 0.0 0.0 0.0 0.7203573118484106 0.0014215574447978782 44-45 0.0 0.0 0.0 0.924350805176906 0.001523097262283441 46-47 0.0 0.0 0.0 1.1598216419259262 0.0015907904739404827 48-49 0.0 0.0 0.0 1.389775481924897 0.0017600235030830872 50-51 0.0 0.0 0.0 1.6215908852444367 0.0017600235030830872 52-53 0.0 0.0 0.0 1.8337752571834343 0.001963103138054213 54-55 3.384660582852091E-5 0.0 0.0 2.066944524736115 0.001963103138054213 56-57 6.769321165704182E-5 3.384660582852091E-5 0.0 2.269787233466441 0.001963103138054213 58-59 6.769321165704182E-5 1.3538642331408364E-4 0.0 2.4038874857590407 0.001963103138054213 60-61 6.769321165704182E-5 1.3538642331408364E-4 0.0 2.502414955325865 0.001963103138054213 62-63 1.0153981748556273E-4 1.3538642331408364E-4 0.0 2.5841206617959145 0.0020307963497112544 64-65 1.3538642331408364E-4 1.3538642331408364E-4 0.0 2.672392609796697 0.0020307963497112544 66-67 1.3538642331408364E-4 1.3538642331408364E-4 0.0 2.7494274846624105 0.0020307963497112544 68-69 1.3538642331408364E-4 1.3538642331408364E-4 0.0 2.833130140876343 0.0020307963497112544 70-71 1.3538642331408364E-4 1.3538642331408364E-4 0.0 2.989399919986624 0.0020307963497112544 72-73 1.3538642331408364E-4 1.3538642331408364E-4 0.0 3.183848670471477 0.002166182773025338 74-75 1.3538642331408364E-4 1.3538642331408364E-4 0.0 3.407879354450456 0.002301569196339422 76-77 1.3538642331408364E-4 1.3538642331408364E-4 0.0 3.744246923174297 0.002301569196339422 78-79 1.3538642331408364E-4 1.3538642331408364E-4 0.0 4.231401120864199 0.0025046488313105473 80-81 1.3538642331408364E-4 1.3538642331408364E-4 0.0 4.772405268427277 0.0026061886487961103 82-83 1.3538642331408364E-4 1.3538642331408364E-4 0.0 5.2807135947600035 0.0029108081012527982 84-85 1.3538642331408364E-4 1.3538642331408364E-4 0.0 5.682134339886262 0.0029108081012527982 86-87 1.3538642331408364E-4 1.3538642331408364E-4 0.0 6.033055949116367 0.0029446547070813194 88-89 1.3538642331408364E-4 2.0307963497112546E-4 0.0 6.399479303815934 0.00297850131290984 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2675 0.0 46.530033 1 GTATCAA 4320 0.0 35.68759 1 TCAACGC 5100 0.0 29.666777 4 ATCAACG 5180 0.0 29.208603 3 CAACGCA 5245 0.0 28.846628 5 TATCAAC 5420 0.0 27.91618 2 AACGCAG 5490 0.0 27.452991 6 TACATGG 4585 0.0 24.878618 2 GTACATG 4850 0.0 24.20532 1 ACATGGG 4575 0.0 24.10795 3 ACGCAGA 6275 0.0 23.868515 7 CGCAGAG 6295 0.0 23.792683 8 GAGTACT 4210 0.0 20.976767 12-13 GCAGAGT 7225 0.0 20.143394 9 CATGGGG 2555 0.0 20.108105 4 CAGAGTA 6675 0.0 19.79218 10-11 TAGGACA 810 0.0 19.784697 4 GAGTACA 3475 0.0 19.537136 1 GTACTTT 4570 0.0 19.479254 14-15 AGAGTAC 5995 0.0 19.405273 10-11 >>END_MODULE