FastQCFastQC Report
Fri 27 May 2016
SRR939758_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939758_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1581881
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35990.22751395332518692No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28770.18187208772341282No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18190.11498968632912338No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN15860.10026038621109931No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA23750.043.572781
GTATCAA41650.039.3025251
ATCAACG51900.032.0842673
TCAACGC52150.031.9314674
CAACGCA53050.031.6537575
TATCAAC52650.031.448542
AACGCAG54900.030.4147826
TACATGG45850.026.8793132
ACGCAGA62350.026.5543027
CGCAGAG64050.025.5557618
GTACATG48500.025.5076351
ACATGGG47400.024.809493
GCAGAGT70050.022.0910559
GTATACG1554.2128937E-521.2433261
AGAGTAC57600.021.02663210-11
GAGTACT40900.020.7581912-13
CATGGGG27900.019.8951744
CAGAGTA64850.019.47373610-11
GTAGGAC3900.019.2979683
GTACTTT44450.018.89527714-15
Produced by FastQC (version 0.11.5)