FastQCFastQC Report
Fri 27 May 2016
SRR939758_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939758_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1581881
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39230.24799589855368387No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36350.2297897250172421No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23270.14710335353923587No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA26700.047.103881
GTATCAA43300.036.060361
ATCAACG53000.028.9114743
TCAACGC53050.028.8842264
TATCAAC55600.027.7327772
CAACGCA55350.027.6839775
AACGCAG58800.026.0596626
ACGCAGA66600.022.93647
CGCAGAG67500.022.5602998
GTACATG48900.021.837141
TACATGG47450.021.5974432
GGGCGTA2257.252311E-821.084399
ACATGGG46350.021.084393
TAAGGTG9500.020.9734235
GTAAGGT8450.020.7724934
TGGTATC14700.020.0105552
GTGTAAG6950.019.8031961
GTGGTAT16750.019.5504321
ACACCGT3404.3655746E-1119.534076
GCAGAGT76850.019.4451039
Produced by FastQC (version 0.11.5)