##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939758_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1581881 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.338027955326602 31.0 31.0 34.0 30.0 34.0 2 31.572040501150212 31.0 31.0 34.0 30.0 34.0 3 31.61423520479733 31.0 31.0 34.0 30.0 34.0 4 35.431277700408565 37.0 35.0 37.0 33.0 37.0 5 35.1590265007292 37.0 35.0 37.0 32.0 37.0 6 35.181390382715264 37.0 35.0 37.0 32.0 37.0 7 35.0851024824244 37.0 35.0 37.0 32.0 37.0 8 35.04386107425274 37.0 35.0 37.0 32.0 37.0 9 36.63357610338578 39.0 35.0 39.0 32.0 39.0 10-11 36.49354597469721 38.0 35.0 39.0 32.0 39.0 12-13 36.41044869999703 38.0 35.0 39.0 32.0 39.0 14-15 37.44318883658126 39.0 36.0 41.0 32.0 41.0 16-17 37.35189720339267 39.0 36.0 41.0 32.0 41.0 18-19 37.305593151444384 39.0 36.0 40.0 31.5 41.0 20-21 37.208337099946206 39.0 36.0 40.0 31.0 41.0 22-23 37.08503642182946 39.0 36.0 40.0 31.0 41.0 24-25 36.92156868942733 39.0 36.0 40.0 31.0 41.0 26-27 36.61172585042743 38.5 35.0 40.0 30.0 41.0 28-29 36.37744305671539 38.0 35.0 40.0 30.0 41.0 30-31 36.106480828836055 38.0 34.5 40.0 29.5 41.0 32-33 36.09943352249632 38.0 35.0 40.0 30.0 41.0 34-35 35.944236007639006 38.0 34.5 40.0 29.0 41.0 36-37 35.99579266708432 38.0 35.0 40.0 29.0 41.0 38-39 35.890589431189824 38.0 35.0 40.0 29.0 41.0 40-41 35.809357657118326 38.0 34.5 40.0 29.0 41.0 42-43 35.59458739310985 38.0 34.0 40.0 27.5 41.0 44-45 35.3565830805225 38.0 34.0 40.0 27.0 41.0 46-47 35.080989657249816 38.0 33.5 40.0 26.5 41.0 48-49 34.78313254916141 38.0 33.0 40.0 26.0 41.0 50-51 34.551977361128934 38.0 33.0 40.0 24.5 41.0 52-53 34.287929054081815 37.5 33.0 40.0 24.0 41.0 54-55 34.00872347540681 37.0 33.0 40.0 24.0 41.0 56-57 33.61940531557052 37.0 32.0 40.0 23.0 41.0 58-59 33.23072247533158 36.0 31.5 39.0 22.5 41.0 60-61 32.90147805049811 36.0 31.0 39.0 22.0 40.0 62-63 32.59571800912964 35.5 31.0 39.0 21.0 40.0 64-65 32.14237448961079 35.0 31.0 38.5 19.5 40.0 66-67 31.176293602363263 34.5 29.5 38.0 13.0 40.0 68-69 29.162712302632123 34.0 26.0 37.0 7.5 39.0 70-71 28.484805747082113 33.0 24.5 36.0 7.5 39.0 72-73 29.068250709124136 33.0 26.0 36.0 7.5 39.0 74-75 29.20097940363403 33.0 26.5 36.0 8.0 38.5 76-77 28.80802348596386 32.5 27.0 35.0 7.5 37.0 78-79 29.357814525871415 33.5 29.0 35.0 7.0 37.0 80-81 29.16749426790005 34.0 29.0 35.0 6.5 37.0 82-83 28.714298989620584 33.0 27.5 35.0 2.0 36.5 84-85 28.35014485918979 33.0 27.0 35.0 2.0 36.0 86-87 27.94644887953013 33.0 26.5 35.0 2.0 35.5 88-89 27.467471320535488 32.5 26.0 35.0 2.0 35.0 90-91 26.95082626316392 32.0 25.0 34.5 2.0 35.0 92-93 26.42863432837236 32.0 25.0 34.0 2.0 35.0 94-95 25.55316771615564 32.0 22.0 34.0 2.0 35.0 96-97 24.995404521579054 31.0 19.5 34.0 2.0 35.0 98-99 24.28332377719942 31.0 10.0 34.0 2.0 35.0 100-101 22.76517418187588 30.0 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.26579775088144686 1101 2 0.25928055275700146 1101 3 0.334780310446817 1101 4 0.25949239896045384 1101 5 0.2715664398203401 1101 6 0.18697940395527013 1101 7 0.051393457731577996 1101 8 0.21562973389563211 1101 9 0.4028508567238873 1101 10-11 0.31755507792889404 1101 12-13 0.3148317668929721 1101 14-15 0.37224501089760764 1101 16-17 0.40527250192094044 1101 18-19 0.2534114886321319 1101 20-21 0.3198950214548901 1101 22-23 0.31606260564726085 1101 24-25 0.30219546176525114 1101 26-27 0.05816667915406981 1101 28-29 0.25886751897773763 1101 30-31 0.07643592564137691 1101 32-33 0.02414296934867366 1101 34-35 0.1881626941868504 1101 36-37 0.44661812297156445 1101 38-39 0.38515890382419116 1101 40-41 0.4767840856658623 1101 42-43 0.13058393491009213 1101 44-45 0.13200936099549665 1101 46-47 0.24597847513930304 1101 48-49 0.3324656736467304 1101 50-51 0.4063449780227586 1101 52-53 0.12758681876347566 1101 54-55 -0.06158713442209063 1101 56-57 0.02583011479029551 1101 58-59 -0.19767944946557492 1101 60-61 -0.1510807694287024 1101 62-63 -0.14136499085118004 1101 64-65 -0.39592090651272116 1101 66-67 -0.6560974698383717 1101 68-69 -8.806447580622347 1101 70-71 -6.972244845064349 1101 72-73 -2.227204973773791 1101 74-75 -0.8429515540848165 1101 76-77 -0.7977217277565423 1101 78-79 -0.801736567840031 1101 80-81 -0.7607333004195489 1101 82-83 -0.8074068724679577 1101 84-85 -0.6927056542971322 1101 86-87 -0.7744550297066475 1101 88-89 -0.6806591236612043 1101 90-91 -0.9805400582000452 1101 92-93 -1.5031469650517302 1101 94-95 -1.1478066294243412 1101 96-97 -1.4144768676390527 1101 98-99 -0.9541982356418792 1101 100-101 -0.7919269959414414 1102 1 0.26599066957288287 1102 2 0.21263176088400115 1102 3 0.2740123256894975 1102 4 0.20060344225772297 1102 5 0.30771799955041956 1102 6 0.25884898250158983 1102 7 0.38105116385116844 1102 8 0.40250691343839406 1102 9 0.4639211976848756 1102 10-11 0.45398893031872944 1102 12-13 0.2826399509949269 1102 14-15 0.38065644122484343 1102 16-17 0.5126247384799427 1102 18-19 0.44632439250741385 1102 20-21 0.3715204140444257 1102 22-23 0.5631884679061159 1102 24-25 0.3059936800383696 1102 26-27 0.3603559204644853 1102 28-29 0.3653458338645308 1102 30-31 0.304773787302544 1102 32-33 0.23275875318433492 1102 34-35 0.2260599926937985 1102 36-37 0.5552329246756997 1102 38-39 0.675428049952032 1102 40-41 0.290055628896134 1102 42-43 0.2719861451269239 1102 44-45 0.36077480062913025 1102 46-47 0.4937635931809865 1102 48-49 0.4756741289572872 1102 50-51 0.4213617689641467 1102 52-53 0.5115711299139676 1102 54-55 0.3761625637478474 1102 56-57 0.36710187689558893 1102 58-59 0.2784144170529359 1102 60-61 0.1787186442387778 1102 62-63 0.3178993505489629 1102 64-65 0.3676211087229504 1102 66-67 0.5585845545591788 1102 68-69 -4.058651939742948 1102 70-71 0.4392268720032071 1102 72-73 0.9623716644040492 1102 74-75 0.7717249282848684 1102 76-77 0.5503276187401589 1102 78-79 0.6242696434403108 1102 80-81 0.48963710346800937 1102 82-83 0.5067152668109216 1102 84-85 0.5117985060821404 1102 86-87 0.38081820980506365 1102 88-89 0.34218315092733675 1102 90-91 0.10914117698467507 1102 92-93 0.19689463523337025 1102 94-95 0.45198757894608477 1102 96-97 0.0792511703217329 1102 98-99 0.5624524012353369 1102 100-101 0.4037730568832032 1103 1 0.18639014003177223 1103 2 0.24224908557230407 1103 3 0.20590482827933698 1103 4 0.21796877763846112 1103 5 0.29410899057701556 1103 6 0.2719971834449382 1103 7 0.29506248011085034 1103 8 0.27635771607706516 1103 9 0.28098040975254435 1103 10-11 0.3666859204589912 1103 12-13 0.31991406332200256 1103 14-15 0.3174223592137224 1103 16-17 0.392237582110063 1103 18-19 0.3289293127798629 1103 20-21 0.27788923265401166 1103 22-23 0.31304574443892363 1103 24-25 0.26045413240648685 1103 26-27 0.22612494061583988 1103 28-29 0.32415097205048937 1103 30-31 0.3224540316931055 1103 32-33 0.2875864406195845 1103 34-35 0.1232242943967421 1103 36-37 0.2824606809851744 1103 38-39 0.2812245986198505 1103 40-41 0.15740360458691782 1103 42-43 0.21460249033353307 1103 44-45 0.16387428153205974 1103 46-47 0.22416231562071687 1103 48-49 0.202740990579386 1103 50-51 0.10588451156934298 1103 52-53 0.0683793039286229 1103 54-55 0.14899584862460102 1103 56-57 0.2917147769799868 1103 58-59 0.2223166848212088 1103 60-61 0.18836897350835358 1103 62-63 0.052076275428078134 1103 64-65 -0.1196403366758858 1103 66-67 0.02784837668304263 1103 68-69 -4.801708593020539 1103 70-71 0.2764178554084715 1103 72-73 0.6265301146212572 1103 74-75 0.3812601613986928 1103 76-77 0.12686197540133648 1103 78-79 -0.10436364792867892 1103 80-81 -5.774298386036492E-4 1103 82-83 -0.05967954899288941 1103 84-85 -0.1415979948818169 1103 86-87 -0.1835490153825745 1103 88-89 -0.29731783561114256 1103 90-91 -0.30265130979796595 1103 92-93 -0.2585348900708482 1103 94-95 -0.3255303523434172 1103 96-97 -0.5335310657756978 1103 98-99 -0.07748134261467499 1103 100-101 -0.20082560261000637 1104 1 0.10280421771917503 1104 2 0.11763282207739678 1104 3 0.12389440950391162 1104 4 0.1549874604412409 1104 5 0.22799885161379052 1104 6 0.22802035599949022 1104 7 -0.002699368923671841 1104 8 0.15667144406854305 1104 9 0.46469348161352997 1104 10-11 0.21122029243451124 1104 12-13 0.11659489854339711 1104 14-15 0.2620897737101586 1104 16-17 0.31355409297116665 1104 18-19 0.05035619554991655 1104 20-21 0.27126795805258297 1104 22-23 0.27990210704669494 1104 24-25 0.3497409856700813 1104 26-27 0.38048095657816816 1104 28-29 0.37834188078845443 1104 30-31 0.23912965336703706 1104 32-33 0.1450839655711249 1104 34-35 0.172350886539256 1104 36-37 0.06286009973009499 1104 38-39 0.2778945491743414 1104 40-41 0.0913690343302136 1104 42-43 0.08344780049801415 1104 44-45 0.14472628056699932 1104 46-47 0.22959944938980925 1104 48-49 0.21565630648431977 1104 50-51 0.3403677949395103 1104 52-53 0.10303240890118559 1104 54-55 0.1575992418675085 1104 56-57 0.2613803542555786 1104 58-59 0.05568578040938377 1104 60-61 0.001499521568653961 1104 62-63 0.09562638233353482 1104 64-65 0.18301545271898334 1104 66-67 0.2613685056906121 1104 68-69 -4.171251180493051 1104 70-71 0.47458552226278883 1104 72-73 0.7514285475065385 1104 74-75 0.4555467997421161 1104 76-77 0.38559189423084206 1104 78-79 0.05740208769454824 1104 80-81 0.17720852492000816 1104 82-83 0.02110097175175696 1104 84-85 0.19293114968042602 1104 86-87 0.07449506543850148 1104 88-89 -0.19434438033256285 1104 90-91 -0.17583194119184142 1104 92-93 -0.20580682510117398 1104 94-95 0.13074656467520995 1104 96-97 -0.24808726254828883 1104 98-99 0.09218097619374888 1104 100-101 0.0651490326496571 1105 1 0.09837389087807935 1105 2 0.08597792782010316 1105 3 0.09431884317439554 1105 4 0.20022729716001209 1105 5 0.2663838720935985 1105 6 0.15681155943229896 1105 7 0.06351486106323279 1105 8 0.12129396071710374 1105 9 0.20851087691571024 1105 10-11 0.16950695408571903 1105 12-13 0.056642510625096065 1105 14-15 0.11618956751884468 1105 16-17 0.1859241088534631 1105 18-19 0.03665753440026975 1105 20-21 -0.03595640038593473 1105 22-23 0.11132068237618142 1105 24-25 0.20795328609639796 1105 26-27 0.07734785869303096 1105 28-29 0.0324730212493165 1105 30-31 0.13250819553873328 1105 32-33 0.11738501881864494 1105 34-35 0.08726457875692972 1105 36-37 -0.03408186020192261 1105 38-39 0.12807914612606197 1105 40-41 0.00130452988595664 1105 42-43 -0.11097441104313077 1105 44-45 -0.10542017795789604 1105 46-47 -0.06103713145668621 1105 48-49 -0.21375782458648018 1105 50-51 -0.1851544751153824 1105 52-53 -0.07699420092355069 1105 54-55 0.0887315177737733 1105 56-57 -0.04776276759234577 1105 58-59 0.17988974959214232 1105 60-61 0.09593357517839962 1105 62-63 0.07092783263863822 1105 64-65 -0.01690252354115529 1105 66-67 0.5700341135370302 1105 68-69 1.6075994074308255 1105 70-71 1.855277029409823 1105 72-73 1.162609724190201 1105 74-75 0.524991824680118 1105 76-77 0.4855253696689985 1105 78-79 0.5555240749329791 1105 80-81 0.4731700312311382 1105 82-83 0.389018543988378 1105 84-85 0.07585715068351462 1105 86-87 -0.05272358094340035 1105 88-89 0.1479385963455293 1105 90-91 0.17011302105285964 1105 92-93 0.1893704924613111 1105 94-95 0.5641601802677307 1105 96-97 0.1062765397309633 1105 98-99 0.5086687927949392 1105 100-101 0.5804554287598869 1106 1 0.3146255291480671 1106 2 0.3280323183836664 1106 3 0.3011504290197422 1106 4 0.25740838060256976 1106 5 0.24318596907918533 1106 6 0.3798838617477216 1106 7 0.3610847605826706 1106 8 0.3363059746970052 1106 9 0.40669239723025896 1106 10-11 0.36896086409008433 1106 12-13 0.3172939959898784 1106 14-15 0.5520622193318587 1106 16-17 0.4789614614091633 1106 18-19 0.5484011997563201 1106 20-21 0.494286063572126 1106 22-23 0.3535525521883258 1106 24-25 0.5293245180754269 1106 26-27 0.4330244641976151 1106 28-29 0.7461634974397953 1106 30-31 0.7504800172949615 1106 32-33 0.8255927514529802 1106 34-35 0.7201446573938881 1106 36-37 0.8963025871549988 1106 38-39 1.0050887573099843 1106 40-41 0.7835566050279326 1106 42-43 0.8015938502063236 1106 44-45 0.9074702763889775 1106 46-47 1.052071758888438 1106 48-49 0.8548283484148271 1106 50-51 0.8921582160161137 1106 52-53 0.8145414041441654 1106 54-55 0.6971167296566918 1106 56-57 0.659571221048985 1106 58-59 0.8398695442623065 1106 60-61 0.874376672600853 1106 62-63 0.8628265875534495 1106 64-65 0.847604357192786 1106 66-67 1.4684832179494371 1106 68-69 2.896953382965993 1106 70-71 3.230162350510742 1106 72-73 2.3642780291268544 1106 74-75 1.7791148041471345 1106 76-77 1.2484076326659412 1106 78-79 1.0638901736660493 1106 80-81 1.3954013549532824 1106 82-83 1.3492877663562268 1106 84-85 1.2043712398054076 1106 86-87 1.3880802635301706 1106 88-89 1.4792213617452639 1106 90-91 1.3249322652643052 1106 92-93 1.2168852368911942 1106 94-95 1.7119184808356671 1106 96-97 1.3999582784815061 1106 98-99 1.5211933668447415 1106 100-101 1.5037488975235398 1107 1 0.1710431570957347 1107 2 0.1333237271820309 1107 3 0.1712197648333813 1107 4 0.1269923331992473 1107 5 0.16353027407870968 1107 6 0.24974993825777858 1107 7 0.15083171088008385 1107 8 0.15209125482084573 1107 9 0.056964439482172224 1107 10-11 0.0875442930908008 1107 12-13 0.06816841751390967 1107 14-15 0.3625989377185377 1107 16-17 0.20116249949507647 1107 18-19 0.1942161719992228 1107 20-21 0.10606770454139536 1107 22-23 0.27704575031899026 1107 24-25 0.3177348351924607 1107 26-27 0.3103481245561994 1107 28-29 0.32760624823738027 1107 30-31 0.15788483502827688 1107 32-33 0.3127634307293121 1107 34-35 0.34473976486114566 1107 36-37 0.15620822605590234 1107 38-39 0.13175101189469984 1107 40-41 0.0427378277708641 1107 42-43 0.08063391342809467 1107 44-45 0.12188323099874054 1107 46-47 0.01818640038781183 1107 48-49 0.10809391238621657 1107 50-51 -0.15558089009824982 1107 52-53 -0.2626759922505073 1107 54-55 -0.21550087617635683 1107 56-57 -0.022994659356498914 1107 58-59 -0.02745619747154393 1107 60-61 0.1826189999686605 1107 62-63 0.19514132076341895 1107 64-65 0.0022241159483868955 1107 66-67 0.44148631724729626 1107 68-69 1.9086237191406212 1107 70-71 2.002126550856122 1107 72-73 1.0983542887654103 1107 74-75 0.49336149264656015 1107 76-77 0.3450801965625381 1107 78-79 0.2591959407016198 1107 80-81 0.21422373559129326 1107 82-83 0.33329288523852796 1107 84-85 0.278894637390362 1107 86-87 0.23997699804750283 1107 88-89 0.2703264907092269 1107 90-91 0.3845811470129199 1107 92-93 0.3295978054708826 1107 94-95 0.7291910499356966 1107 96-97 0.5023432418460487 1107 98-99 0.6073549856343554 1107 100-101 0.5316466434591653 1108 1 -0.08484437784204601 1108 2 -0.06952525858457292 1108 3 -0.044743380227977525 1108 4 -0.009387236385748565 1108 5 -0.0035046037428330123 1108 6 0.011938573063027036 1108 7 0.022292963382227526 1108 8 0.06078103807592328 1108 9 -0.1502008419725911 1108 10-11 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0.8342844234816802 2316 74-75 0.3272388999808271 2316 76-77 -0.04236431561687937 2316 78-79 0.0023027864175730883 2316 80-81 -0.07123802610944807 2316 82-83 -0.14073409236027956 2316 84-85 -0.5963221622181116 2316 86-87 -0.4323660261567781 2316 88-89 -0.42050245654621676 2316 90-91 -0.2027974666716439 2316 92-93 -0.3422811387794198 2316 94-95 -0.1347204013766934 2316 96-97 -0.03335313566861231 2316 98-99 -0.2043904014710023 2316 100-101 -0.35288717298669425 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 19.0 4 30.0 5 62.0 6 161.0 7 454.0 8 1013.0 9 1667.0 10 2663.0 11 4122.0 12 5595.0 13 7277.0 14 8742.0 15 10803.0 16 12854.0 17 14791.0 18 14166.0 19 13168.0 20 13287.0 21 13851.0 22 14822.0 23 16976.0 24 20298.0 25 24739.0 26 30295.0 27 37347.0 28 45466.0 29 55424.0 30 65435.0 31 77310.0 32 92339.0 33 112056.0 34 137410.0 35 172060.0 36 210767.0 37 219498.0 38 116632.0 39 8278.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 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22.91445278546029 18-19 22.445651727279106 26.87623152436877 28.3171110848414 22.36100566351072 20-21 22.884369936803083 26.157435357021168 28.59225820399891 22.36593650217684 22-23 22.57255760705135 26.12601706449474 28.536280541962384 22.765144786491526 24-25 22.52166568998008 26.364879993981837 28.60873050340766 22.504723812630424 26-27 22.339709307177046 26.585680546389224 28.34454154434355 22.730068602090174 28-29 22.325411741353165 26.8792682626168 28.333993940118052 22.461326055911986 30-31 22.49224653796699 26.45469866752978 28.305067280959168 22.747987513544064 32-33 22.199836203460464 26.46376167508671 28.353332715909097 22.98306940554373 34-35 22.765524398180943 26.630056672473312 28.209546225096148 22.394872704249597 36-37 22.868904924912744 26.25059206973023 28.207483381706126 22.673019623650895 38-39 22.362518512097207 26.514163791049718 28.325051071399987 22.798266625453092 40-41 22.688833672468185 26.383035811185152 28.472646514325454 22.455484002021205 42-43 22.481872060745722 26.39577301102089 28.18146639105217 22.94088853718122 44-45 22.4658723534582 26.57276989363526 28.290146850283836 22.6712109026227 46-47 22.774111557089746 26.66500887897169 27.853237700496607 22.707641863441953 48-49 22.512045962901915 26.835779436283737 27.74681173496667 22.905362865847678 50-51 22.49887292083554 26.877191872375093 27.786764531411894 22.83717067537747 52-53 22.705487598119348 26.730057290058756 27.70944779873577 22.855007313086123 54-55 22.38210286978664 26.781175171255136 27.850248734661836 22.986473224296393 56-57 22.4786115075749 26.95010121156664 27.606158317529232 22.965128963329224 58-59 22.360894830314678 26.714319042678497 27.69009580422631 23.234690322780516 60-61 22.190235435325697 26.88474862484388 27.852992562887653 23.07202337694277 62-63 22.22690030530814 26.78294231828729 27.919590073486994 23.070567302917574 64-65 21.973818926198554 26.851895461569995 27.797816589607415 23.37646902262403 66-67 21.83312859350067 26.887078594767104 27.844469491654213 23.435323320078012 68-69 22.045452452967602 26.502819070562328 28.198515650373658 23.253212826096412 70-71 21.957082313388117 26.45123180603943 27.992576407016546 23.59910947355591 72-73 21.988025067171353 26.572113721958097 27.78107142268992 23.658789788180627 74-75 22.14878285445631 26.965007203943532 27.106683179194974 23.779526762405183 76-77 22.19478432654651 26.75933649055332 27.2743714385629 23.77150774433727 78-79 22.304582891852046 26.740458314472914 27.220292629390897 23.734666164284143 80-81 22.34968513222929 26.766621076717236 27.097593362727103 23.786100428326375 82-83 22.293954754455065 26.86601602713734 27.064356039761563 23.775673178646034 84-85 22.25676634169346 26.775299423342187 26.957867693634256 24.010066541330094 86-87 22.327193717767337 26.720445197988695 26.97751631309511 23.974844771148867 88-89 22.250304227578034 26.707862504938763 26.80998814697748 24.231845120505728 90-91 22.170785063584734 26.73782948044571 26.840398251305185 24.250987204664366 92-93 22.24596395015291 26.447691371585265 26.94221119425984 24.36413348400199 94-95 21.939371239252576 26.230431301439655 27.358232416681876 24.47196504262589 96-97 22.190299394534115 25.935829215119956 27.242676998872025 24.631194391473908 98-99 22.069070606091547 26.163224285518734 26.940925382108894 24.82677972628083 100-101 21.94229594759143 25.943083694647495 27.18840051943257 24.92621983832851 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 354.0 1 249.5 2 180.0 3 228.0 4 210.0 5 233.0 6 367.0 7 665.0 8 1000.5 9 1186.5 10 1147.5 11 999.0 12 1007.0 13 1077.0 14 1139.5 15 1255.5 16 1380.5 17 1507.5 18 1692.0 19 1970.0 20 2340.0 21 2831.5 22 3505.5 23 4239.5 24 5196.5 25 6530.5 26 8381.5 27 10694.0 28 12698.5 29 14673.0 30 17135.0 31 20225.5 32 24174.0 33 26634.0 34 28097.0 35 30417.0 36 33648.0 37 36881.5 38 39193.0 39 41276.0 40 43435.0 41 45899.5 42 47483.5 43 49327.5 44 52345.0 45 55070.0 46 56570.0 47 57804.5 48 59108.0 49 59446.0 50 59570.0 51 59629.0 52 59678.5 53 59815.0 54 59607.5 55 56443.5 56 51755.5 57 47478.0 58 42576.5 59 37131.5 60 30960.5 61 25539.0 62 20599.0 63 15283.0 64 10985.0 65 8267.5 66 6289.0 67 4821.0 68 3522.5 69 2567.0 70 1903.5 71 1283.0 72 895.5 73 679.5 74 533.5 75 401.5 76 276.0 77 174.5 78 115.0 79 69.5 80 38.0 81 22.0 82 15.0 83 9.0 84 5.5 85 4.5 86 2.0 87 1.0 88 0.5 89 0.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.022631285159882445 2 0.021240535792515367 3 6.321588033486716E-5 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 3.7929528200920297E-4 18-19 0.0 20-21 0.0 22-23 0.0 24-25 1.2643176066973432E-4 26-27 3.160794016743358E-4 28-29 1.2643176066973432E-4 30-31 4.425111623440702E-4 32-33 0.0025602431535621203 34-35 0.004583151324277869 36-37 0.10237811820231738 38-39 0.09453934904079385 40-41 0.10332635640734039 42-43 0.10459067401403772 44-45 0.09886963684373223 46-47 0.09239000910940835 48-49 0.09330663937426392 50-51 0.09283252027175243 52-53 0.07288791002610183 54-55 0.061919954788002385 56-57 0.03413657538082827 58-59 0.01378106191300104 60-61 0.009577205870732375 62-63 0.0023073796322226514 64-65 0.005657821289970611 66-67 0.16695313996438418 68-69 0.4414048844382099 70-71 0.4193109342611739 72-73 0.19489455907239547 74-75 0.02879483349253199 76-77 0.03363084833814933 78-79 0.005373349828463709 80-81 0.0016752208288739797 82-83 9.482382050230074E-5 84-85 4.425111623440702E-4 86-87 0.0014539652477019447 88-89 3.7929528200920297E-4 90-91 9.79846145190441E-4 92-93 0.0027498907945667215 94-95 0.08391908114453617 96-97 0.01826938941677661 98-99 0.018743508519288115 100-101 0.009798461451904411 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1581881.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 65.45986738756449 #Duplication Level Percentage of deduplicated Percentage of total 1 81.55719806359936 53.38723369744546 2 11.418092266716027 14.948536111964136 3 3.1814850759471156 6.247787735010412 4 1.332200607883139 3.488227005026523 5 0.6876225952603777 2.250584194921863 6 0.4267473208488727 1.6760893818459386 7 0.2734139895578168 1.2528350448851742 8 0.1844541099556242 0.965947326142912 9 0.13978178062090887 0.8235087140378093 >10 0.7319352155509402 8.584317141592306 >50 0.043648940837377116 1.9576548811264947 >100 0.020710464423427933 2.5540277761894354 >500 0.001838635970759964 0.7981560641744262 >1k 8.7093282825472E-4 1.0650949256372064 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3923 0.24799589855368387 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3635 0.2297897250172421 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2327 0.14710335353923587 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 6.321588033486716E-5 10-11 0.0 0.0 0.0 3.160794016743358E-5 6.321588033486716E-5 12-13 0.0 0.0 0.0 6.321588033486716E-5 5.057270426789373E-4 14-15 0.0 0.0 0.0 6.321588033486716E-5 6.637667435161051E-4 16-17 0.0 0.0 0.0 6.321588033486716E-5 8.21806444353273E-4 18-19 0.0 0.0 0.0 9.482382050230074E-5 8.21806444353273E-4 20-21 0.0 0.0 0.0 4.109032221766366E-4 9.482382050230074E-4 22-23 0.0 0.0 0.0 6.00550863181238E-4 0.0011378858460276088 24-25 0.0 0.0 0.0 0.0010430620255253082 0.0011378858460276088 26-27 0.0 0.0 0.0 0.0020861240510506164 0.0012643176066973432 28-29 0.0 0.0 0.0 0.00556299746946831 0.0012643176066973432 30-31 0.0 0.0 0.0 0.021746262835194305 0.0012959255468647768 32-33 0.0 0.0 0.0 0.060497597480467874 0.0014539652477019447 34-35 0.0 0.0 0.0 0.12412438103751167 0.001580397008371679 36-37 0.0 0.0 0.0 0.22849379947037735 0.001580397008371679 38-39 0.0 0.0 0.0 0.3878926417347449 0.0017068287690414133 40-41 0.0 0.0 0.0 0.554845781699129 0.0017068287690414133 42-43 0.0 0.0 0.0 0.7191122467492814 0.0017068287690414133 44-45 0.0 0.0 0.0 0.9230782846497303 0.0017068287690414133 46-47 0.0 0.0 0.0 1.172370108750279 0.001738436709208847 48-49 0.0 0.0 0.0 1.4308598434395507 0.0018016525895437141 50-51 0.0 0.0 0.0 1.7061017864175625 0.0018964764100460147 52-53 0.0 0.0 0.0 1.9705970297386464 0.0019280843502134483 54-55 0.0 0.0 0.0 2.2555426103480603 0.002022908170715749 56-57 0.0 0.0 0.0 2.577374657132869 0.002054516110883183 58-59 0.0 0.0 0.0 2.896994148105957 0.0021177319912180498 60-61 0.0 0.0 0.0 3.220216944258133 0.0021493399313854836 62-63 0.0 0.0 0.0 3.5261501971387226 0.002212555811720351 64-65 0.0 0.0 0.0 3.8407756335653565 0.0022757716920552176 66-67 0.0 0.0 0.0 4.184164295544355 0.0022757716920552176 68-69 0.0 0.0 0.0 4.562764202869875 0.0022757716920552176 70-71 0.0 0.0 0.0 4.996393534026896 0.0022757716920552176 72-73 0.0 0.0 0.0 5.438936304311133 0.002307379632222651 74-75 0.0 0.0 0.0 5.878918831441808 0.0023705955125575186 76-77 0.0 0.0 0.0 6.361540469858352 0.0025286352133946865 78-79 0.0 0.0 0.0 6.892743512312241 0.0026234590338969875 80-81 0.0 0.0 0.0 7.458335993668298 0.0026866749142318543 82-83 0.0 0.0 0.0 8.016247745563668 0.0028763225552364555 84-85 0.0 0.0 0.0 8.560694514947711 0.00300275431590619 86-87 0.0 0.0 0.0 9.113169701134282 0.0031924019569107916 88-89 0.0 0.0 0.0 9.68973013772844 0.0033504416577479594 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2670 0.0 47.10388 1 GTATCAA 4330 0.0 36.06036 1 ATCAACG 5300 0.0 28.911474 3 TCAACGC 5305 0.0 28.884226 4 TATCAAC 5560 0.0 27.732777 2 CAACGCA 5535 0.0 27.683977 5 AACGCAG 5880 0.0 26.059662 6 ACGCAGA 6660 0.0 22.9364 7 CGCAGAG 6750 0.0 22.560299 8 GTACATG 4890 0.0 21.83714 1 TACATGG 4745 0.0 21.597443 2 GGGCGTA 225 7.252311E-8 21.08439 9 ACATGGG 4635 0.0 21.08439 3 TAAGGTG 950 0.0 20.973423 5 GTAAGGT 845 0.0 20.772493 4 TGGTATC 1470 0.0 20.010555 2 GTGTAAG 695 0.0 19.803196 1 GTGGTAT 1675 0.0 19.550432 1 ACACCGT 340 4.3655746E-11 19.53407 6 GCAGAGT 7685 0.0 19.445103 9 >>END_MODULE