FastQCFastQC Report
Fri 27 May 2016
SRR939757_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939757_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2335870
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57840.24761651975495214No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47320.20257976685346357No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28150.12051184355293745No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA35750.053.355561
GTATCAA69750.042.4492571
ATCAACG84450.034.8354763
TCAACGC85350.034.5800744
TATCAAC86650.034.443122
CAACGCA86750.033.8032155
AACGCAG90650.032.45366
ACGCAGA100200.028.9342697
CGCAGAG103050.028.1322388
GCAGAGT109350.025.1668919
TACATGG76450.023.6465552
GTACATG77700.023.6330451
TGGTATC19550.022.0858972
ACATGGG78050.022.0072293
GAGTACT66100.021.85661712-13
CAGAGTA104900.021.59754210-11
GTGGTAT20700.021.0885731
AGAGTAC98700.021.0794310-11
GTACTTT70050.020.69344314-15
AGTACTT70300.019.50471512-13
Produced by FastQC (version 0.11.5)