##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939757_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2335870 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.79195503174406 31.0 28.0 33.0 16.0 34.0 2 29.628966509266355 31.0 30.0 33.0 25.0 34.0 3 29.880334093935023 31.0 30.0 34.0 25.0 34.0 4 33.6467603077226 35.0 33.0 37.0 28.0 37.0 5 33.6061356154238 35.0 33.0 37.0 28.0 37.0 6 33.61496787064348 35.0 33.0 37.0 28.0 37.0 7 33.5303201804895 35.0 33.0 37.0 28.0 37.0 8 33.39291313300826 35.0 33.0 37.0 26.0 37.0 9 34.64985080505336 37.0 34.0 39.0 27.0 39.0 10-11 34.48984040207717 37.0 33.5 39.0 27.0 39.0 12-13 34.365769713211776 37.0 33.0 39.0 27.0 39.0 14-15 35.04013943412947 38.0 33.0 40.0 26.0 41.0 16-17 34.86060975139884 38.0 33.0 40.0 25.5 41.0 18-19 34.69887215470039 38.0 33.0 40.0 25.0 41.0 20-21 34.88185515461049 38.0 33.0 40.0 25.0 41.0 22-23 34.78149533150389 38.0 33.0 40.0 25.0 41.0 24-25 34.5766491714008 38.0 33.0 40.0 24.5 41.0 26-27 34.46052198966552 38.0 33.0 40.0 24.0 41.0 28-29 34.50612105981925 38.0 33.0 40.0 24.0 41.0 30-31 34.27807690496475 38.0 33.0 40.0 23.5 41.0 32-33 33.923366454468784 38.0 32.5 40.0 21.5 41.0 34-35 33.734549439823276 38.0 32.0 40.0 20.0 41.0 36-37 33.52807733307076 38.0 32.0 40.0 18.5 41.0 38-39 33.310759588504496 37.5 31.5 40.0 18.0 41.0 40-41 33.069541541267284 37.0 31.0 40.0 16.5 41.0 42-43 32.68025960348821 37.0 31.0 40.0 15.0 41.0 44-45 32.34533107578761 37.0 30.0 40.0 12.5 41.0 46-47 31.976223419967724 37.0 30.0 39.5 9.0 41.0 48-49 31.624650986570316 36.0 30.0 39.0 8.5 40.5 50-51 31.005324996682177 35.5 29.0 38.5 8.0 40.0 52-53 31.162759271706044 36.0 30.0 39.0 7.5 40.0 54-55 31.25505143693784 36.0 29.5 39.0 4.0 40.0 56-57 30.947065761365145 36.0 29.0 39.0 2.0 40.0 58-59 30.543092723482044 35.0 28.0 39.0 2.0 40.0 60-61 30.136285409718866 35.0 28.0 39.0 2.0 40.0 62-63 29.67476550492964 34.5 27.0 38.0 2.0 40.0 64-65 29.122454588654335 34.0 26.0 38.0 2.0 40.0 66-67 28.52963285628053 34.0 26.0 37.5 2.0 40.0 68-69 27.96804060157457 33.5 25.0 37.0 2.0 39.0 70-71 27.40348970619084 33.0 23.5 36.0 2.0 39.0 72-73 26.748181619696304 32.0 22.0 36.0 2.0 38.5 74-75 26.3927104248096 32.0 22.0 35.0 2.0 38.0 76-77 25.814399773960023 32.0 20.0 35.0 2.0 37.5 78-79 25.151905927984007 31.0 18.0 35.0 2.0 37.0 80-81 24.42017706464829 31.0 12.0 34.0 2.0 36.0 82-83 23.66964407265815 30.0 7.0 34.0 2.0 36.0 84-85 22.873639800160113 29.5 2.0 34.0 2.0 35.0 86-87 21.909131287271983 29.0 2.0 34.0 2.0 35.0 88-89 20.885936075209663 28.0 2.0 34.0 2.0 35.0 90-91 19.47187450500242 26.0 2.0 32.5 2.0 34.5 92-93 17.920162937149755 22.0 2.0 32.0 2.0 34.0 94-95 17.41683805177514 20.0 2.0 32.0 2.0 34.0 96-97 17.11740657656462 20.0 2.0 32.0 2.0 34.0 98-99 16.232027895388015 10.0 2.0 32.0 2.0 34.0 100-101 14.619092672109321 2.0 2.0 30.0 2.0 34.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.6083879844173303 1101 2 0.9850641287087889 1101 3 0.9725372328354034 1101 4 0.8699054070482504 1101 5 0.8769307530183212 1101 6 0.8908351626909621 1101 7 0.8944636940603701 1101 8 1.0263513037931844 1101 9 1.0012593157437948 1101 10-11 1.0130260187479294 1101 12-13 1.0239463153895656 1101 14-15 1.220013749097241 1101 16-17 1.0987084054067182 1101 18-19 1.1036259586066777 1101 20-21 0.9820109069782887 1101 22-23 1.1463619159421867 1101 24-25 1.3913004683534282 1101 26-27 1.1952746020019944 1101 28-29 1.047830274052771 1101 30-31 1.0631929334851833 1101 32-33 1.1622421910275165 1101 34-35 1.06502501880788 1101 36-37 0.8928288551339136 1101 38-39 1.0203608772639683 1101 40-41 0.9431551603267962 1101 42-43 0.9768991352058407 1101 44-45 1.0521922818647127 1101 46-47 0.8721086439213366 1101 48-49 0.8937828907827416 1101 50-51 0.669999792398901 1101 52-53 0.7012140825191153 1101 54-55 0.9665048688751376 1101 56-57 0.9960065411358165 1101 58-59 0.7499527518407341 1101 60-61 0.5967172188718486 1101 62-63 0.21959545526238244 1101 64-65 0.37876627610069846 1101 66-67 0.7706410395384395 1101 68-69 0.41049404270014733 1101 70-71 0.4176758944587817 1101 72-73 0.48426833432957395 1101 74-75 0.45377777823532384 1101 76-77 0.3245410451603412 1101 78-79 0.1845569330460357 1101 80-81 0.020201855817784775 1101 82-83 -0.3572768871899292 1101 84-85 -0.042868457934048365 1101 86-87 -0.4116899223056656 1101 88-89 -4.354347172853149 1101 90-91 -8.130318745931149 1101 92-93 -10.500511069445706 1101 94-95 -8.397719448359407 1101 96-97 -5.022524305301442 1101 98-99 -3.601245621176316 1101 100-101 -2.7603646158372523 1102 1 2.031959140646048 1102 2 1.2488411338959402 1102 3 1.0746609342045481 1102 4 1.1254393055905112 1102 5 1.1238170934214295 1102 6 1.156948898423444 1102 7 1.17116268374415 1102 8 1.2953395340741451 1102 9 1.702535775493196 1102 10-11 1.3675740968660577 1102 12-13 1.3687839464714173 1102 14-15 1.6308564195724884 1102 16-17 1.578748242675175 1102 18-19 1.5052310953813972 1102 20-21 1.6180661025700118 1102 22-23 1.5866979477253338 1102 24-25 1.7081159906239876 1102 26-27 1.5639399632292381 1102 28-29 1.4153141795013937 1102 30-31 1.6266644333472087 1102 32-33 1.9757838830852492 1102 34-35 2.072787989768919 1102 36-37 1.7891862036775166 1102 38-39 1.7113797971076394 1102 40-41 1.5815582209171808 1102 42-43 1.761148187046949 1102 44-45 1.6751959420905536 1102 46-47 1.6121977014921782 1102 48-49 1.3817098331204214 1102 50-51 1.4663377007888236 1102 52-53 1.6535456821879677 1102 54-55 1.5183073111723395 1102 56-57 1.5036917213890781 1102 58-59 1.496599851962639 1102 60-61 1.2546334089387763 1102 62-63 1.3774034605732126 1102 64-65 1.2577811117065139 1102 66-67 1.3321767996409228 1102 68-69 1.1897515492025157 1102 70-71 1.162284787757315 1102 72-73 1.0293463191379644 1102 74-75 1.1363295481770557 1102 76-77 1.1223062700958586 1102 78-79 1.1120216872349218 1102 80-81 1.2469100088557319 1102 82-83 0.8895030740441179 1102 84-85 0.9673990127003336 1102 86-87 0.9168190329545958 1102 88-89 0.3039608295988394 1102 90-91 -5.659111990868961 1102 92-93 -10.292855019329831 1102 94-95 -8.165213254443572 1102 96-97 -4.57737232151802 1102 98-99 -2.9867440188682934 1102 100-101 -2.183754912432006 1103 1 2.1834583760816066 1103 2 1.4332796555269383 1103 3 1.0265688065176306 1103 4 0.9387403055774755 1103 5 1.057648588757992 1103 6 1.1049045033978615 1103 7 0.9734934412123906 1103 8 1.0527338934945547 1103 9 1.133361474046822 1103 10-11 1.0627938152406173 1103 12-13 0.9376329277661313 1103 14-15 1.2113175919294648 1103 16-17 1.1372912802341304 1103 18-19 1.1183543488809207 1103 20-21 0.923984383886264 1103 22-23 1.1529570950264443 1103 24-25 1.0478228672339043 1103 26-27 1.1379000096929062 1103 28-29 0.7556856653142745 1103 30-31 1.0569593137144508 1103 32-33 1.2082726257042324 1103 34-35 1.166535137785253 1103 36-37 1.2235994260791614 1103 38-39 1.1593232957023716 1103 40-41 1.0438325688644667 1103 42-43 1.1140631238982124 1103 44-45 1.01273267253611 1103 46-47 1.100871717662578 1103 48-49 1.0189086403626355 1103 50-51 0.984477713863722 1103 52-53 0.7741984830144304 1103 54-55 0.9860009713406761 1103 56-57 0.9945781008427481 1103 58-59 1.063408753069659 1103 60-61 0.8225241237180576 1103 62-63 0.6176722594527249 1103 64-65 0.784456432882557 1103 66-67 0.8604566817558279 1103 68-69 0.9589635977199187 1103 70-71 0.8480363859546429 1103 72-73 0.7685944570836405 1103 74-75 0.7460310421043097 1103 76-77 0.5504313147187325 1103 78-79 0.4730550381380283 1103 80-81 0.2672077512952953 1103 82-83 0.04042430745449721 1103 84-85 0.13613524945393962 1103 86-87 0.18588332155578868 1103 88-89 -0.7000653176979306 1103 90-91 -6.37746420705041 1103 92-93 -9.923616957617096 1103 94-95 -8.618106710231384 1103 96-97 -5.269183628379977 1103 98-99 -3.822917074288817 1103 100-101 -3.080146031688537 1104 1 1.9270352369398616 1104 2 1.189705550369741 1104 3 1.0184137092618286 1104 4 0.6799543750136721 1104 5 0.636940153130368 1104 6 0.8123855917326566 1104 7 0.7367220063472999 1104 8 0.8886717199006782 1104 9 0.8709847761127278 1104 10-11 0.8358715226324591 1104 12-13 0.79464511134276 1104 14-15 1.0481064854941593 1104 16-17 0.8962141958085184 1104 18-19 0.9769294594245324 1104 20-21 0.9270729914091902 1104 22-23 0.8776590926252581 1104 24-25 0.7260602781692 1104 26-27 0.8629056677694322 1104 28-29 0.8668894924318948 1104 30-31 0.8542863014682922 1104 32-33 0.8603202093722118 1104 34-35 0.7923663150047346 1104 36-37 0.8392233976915264 1104 38-39 0.7931303607154021 1104 40-41 0.6436619287435548 1104 42-43 0.8246177078469188 1104 44-45 0.7075953168392886 1104 46-47 0.6701183072158443 1104 48-49 0.6937077364215831 1104 50-51 0.7890577956920382 1104 52-53 0.6852090732624845 1104 54-55 0.6253677965757127 1104 56-57 0.8987403294973113 1104 58-59 0.6109429073174475 1104 60-61 0.6839810753117668 1104 62-63 0.5751254796494152 1104 64-65 0.32935764811116996 1104 66-67 0.4358512403662971 1104 68-69 0.4277892406027526 1104 70-71 0.4493836926479915 1104 72-73 0.3011263080987092 1104 74-75 0.16519744075682397 1104 76-77 0.21528490787734 1104 78-79 -0.05425244814169261 1104 80-81 -0.28418002864615133 1104 82-83 -0.39715781910400594 1104 84-85 -0.5201101558590153 1104 86-87 -0.1926201300931254 1104 88-89 -0.5139439349333692 1104 90-91 -3.2595898055042305 1104 92-93 -7.982244319808487 1104 94-95 -5.870792496485343 1104 96-97 -3.188986949550891 1104 98-99 -2.1857012070688064 1104 100-101 -1.6923177321320981 1105 1 1.9567797606828172 1105 2 1.117483413049566 1105 3 0.9811301233349141 1105 4 0.9177557635517175 1105 5 0.8141609734699387 1105 6 0.767917314783773 1105 7 0.8215150606006034 1105 8 0.7341207653369608 1105 9 0.9953422648779409 1105 10-11 0.8059642866106529 1105 12-13 0.8337959330397666 1105 14-15 1.02612918071069 1105 16-17 0.9017730225900564 1105 18-19 0.7791908904116056 1105 20-21 0.8168960125896518 1105 22-23 0.7902971620845207 1105 24-25 0.8669210171346577 1105 26-27 0.6864508536884344 1105 28-29 0.8585336325874025 1105 30-31 1.057214852798623 1105 32-33 1.0505287957261302 1105 34-35 0.8941347547558038 1105 36-37 0.8247580064134468 1105 38-39 0.7160515765087396 1105 40-41 0.8808036583301302 1105 42-43 0.9071546349940647 1105 44-45 0.8599243947820625 1105 46-47 0.996266709803777 1105 48-49 0.7919706704745657 1105 50-51 0.920360761460433 1105 52-53 0.8548927192401266 1105 54-55 0.6595018660997063 1105 56-57 0.6535150359185131 1105 58-59 0.6504560110496733 1105 60-61 0.6157303761434001 1105 62-63 0.6305461807865136 1105 64-65 0.6783931957997993 1105 66-67 0.7738363769142644 1105 68-69 0.7798080864602888 1105 70-71 0.5377812284109815 1105 72-73 0.699973837703805 1105 74-75 0.5060561121076859 1105 76-77 0.542899518900601 1105 78-79 0.08008226989410971 1105 80-81 -0.011386150942858109 1105 82-83 0.10743678010688384 1105 84-85 0.06751202443158277 1105 86-87 -0.006801988483100985 1105 88-89 -0.41884148463028126 1105 90-91 -3.3831633986498186 1105 92-93 -8.140091411618664 1105 94-95 -5.653390553107418 1105 96-97 -3.1058291986481077 1105 98-99 -2.208889504729793 1105 100-101 -1.667477769996415 1106 1 1.6017960414780958 1106 2 1.016407321007545 1106 3 0.6975843598861253 1106 4 0.7805821474193166 1106 5 0.7173334837370007 1106 6 0.8582948685529317 1106 7 0.7583909437569503 1106 8 -0.1591065183458582 1106 9 -0.46373646422758696 1106 10-11 -0.5721268196800153 1106 12-13 -0.3645188037973668 1106 14-15 0.6085534832830035 1106 16-17 0.6784458802122586 1106 18-19 0.7667910208470019 1106 20-21 0.6612695682302743 1106 22-23 0.6742153280493923 1106 24-25 0.7387356287349007 1106 26-27 0.6265668277714909 1106 28-29 0.4821533094289734 1106 30-31 0.6641690444535868 1106 32-33 0.7059501351464519 1106 34-35 0.8086537269626604 1106 36-37 0.7636386249418905 1106 38-39 0.8360672665639868 1106 40-41 0.6423554615050051 1106 42-43 0.7270635192515513 1106 44-45 0.7095479752337681 1106 46-47 0.7003333641830451 1106 48-49 0.6280034494574487 1106 50-51 0.5813199342353528 1106 52-53 0.5493955287997139 1106 54-55 0.7134704576336937 1106 56-57 0.8452834280186643 1106 58-59 0.8783606797862937 1106 60-61 0.43409199410945476 1106 62-63 0.7175763652391822 1106 64-65 0.8555788157696469 1106 66-67 0.6713373102835121 1106 68-69 0.6024305941280907 1106 70-71 0.527892712869793 1106 72-73 0.5098188747786487 1106 74-75 0.3463809718660009 1106 76-77 0.46202862631604447 1106 78-79 0.2385003153480092 1106 80-81 0.2553471511993983 1106 82-83 0.10619764381607055 1106 84-85 0.1901430387558456 1106 86-87 0.06397906435741518 1106 88-89 -0.3566942155032997 1106 90-91 -3.677764507350709 1106 92-93 -8.678519759578721 1106 94-95 -6.229449986280542 1106 96-97 -3.374845592865473 1106 98-99 -2.333123078612468 1106 100-101 -1.7116826102839386 1107 1 1.8176191148935104 1107 2 1.055617940655921 1107 3 0.834383265494882 1107 4 0.8559482111574752 1107 5 0.6104023391861588 1107 6 0.5876813001281462 1107 7 0.5794731750991033 1107 8 0.49877461860504724 1107 9 0.5738933680670897 1107 10-11 0.5245570735155027 1107 12-13 0.43763957893557404 1107 14-15 0.5750833510440643 1107 16-17 0.5945425514388916 1107 18-19 0.6022219373996691 1107 20-21 0.4883760180453436 1107 22-23 0.5445147656538865 1107 24-25 0.38111795634294054 1107 26-27 0.4489993149782592 1107 28-29 0.31740436075389766 1107 30-31 0.5494276957177107 1107 32-33 0.7302547407096043 1107 34-35 0.5188351121782446 1107 36-37 0.31741832803759706 1107 38-39 0.44303641081083356 1107 40-41 0.21314889515753777 1107 42-43 0.40899593045892857 1107 44-45 0.5536124227181887 1107 46-47 0.4161995039205948 1107 48-49 0.17554866909569355 1107 50-51 0.17609624269154978 1107 52-53 0.28325072462481415 1107 54-55 0.13426135797315553 1107 56-57 0.04688954850303517 1107 58-59 0.13789353685676886 1107 60-61 -0.0628604302711473 1107 62-63 -0.003475466346166911 1107 64-65 0.16050357637156054 1107 66-67 0.3956064494637346 1107 68-69 0.2633086404370566 1107 70-71 0.16589494218528955 1107 72-73 0.20233800129230062 1107 74-75 0.1276851214328083 1107 76-77 0.25048685198357035 1107 78-79 0.12197804752615227 1107 80-81 0.05669740706402138 1107 82-83 0.08919151319844687 1107 84-85 0.04165289334367728 1107 86-87 0.18585422268947838 1107 88-89 -0.040985141175895023 1107 90-91 -3.2399953428373607 1107 92-93 -4.708124056077683 1107 94-95 -3.0776817337598956 1107 96-97 -1.8060585431618676 1107 98-99 -1.0972412323339054 1107 100-101 -0.7926650290912551 1108 1 1.6195192217810082 1108 2 0.888342119023136 1108 3 -0.35073965512818006 1108 4 -0.23664230594971514 1108 5 0.2890819153720372 1108 6 0.530284789019106 1108 7 0.45026694449192206 1108 8 0.3695414066876097 1108 9 0.5326254540336919 1108 10-11 0.5694394453802261 1108 12-13 0.5797589608560187 1108 14-15 0.6360569612471494 1108 16-17 0.6768381420701459 1108 18-19 0.47255131693331265 1108 20-21 0.5092694107375593 1108 22-23 0.3254555831875905 1108 24-25 0.5095640404208623 1108 26-27 0.638870946206012 1108 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2311 82-83 0.9987151105728884 2311 84-85 1.446733266948506 2311 86-87 1.569711826073945 2311 88-89 1.934104850954995 2311 90-91 2.5204587253552795 2311 92-93 3.2927801898145326 2311 94-95 3.073696878123929 2311 96-97 2.3232779123574225 2311 98-99 1.9911772398054985 2311 100-101 1.9022962438571884 2312 1 1.2194255805751695 2312 2 0.560310330460652 2312 3 -0.048233748469609594 2312 4 -0.4346430816573914 2312 5 -0.19567311088921713 2312 6 -0.14352735065153865 2312 7 -0.0428275574094954 2312 8 0.0711444328746964 2312 9 0.08961663538086384 2312 10-11 0.02310211452545019 2312 12-13 0.09339202330976093 2312 14-15 0.09522692312621217 2312 16-17 -0.15588289212434603 2312 18-19 -0.5159009144624562 2312 20-21 0.22458476990412635 2312 22-23 0.4562031212428508 2312 24-25 0.3697115948556089 2312 26-27 0.3442249516854119 2312 28-29 0.3470328801705307 2312 30-31 0.3089962404339488 2312 32-33 0.4972990786398839 2312 34-35 0.5302491788752022 2312 36-37 0.4953651730276647 2312 38-39 0.2969601355323661 2312 40-41 0.38257941222847336 2312 42-43 0.5655185643879008 2312 44-45 0.40415223479060813 2312 46-47 0.36737507035292083 2312 48-49 0.4134636692000875 2312 50-51 0.2921197977659027 2312 52-53 0.19495117656435568 2312 54-55 0.5529136488728206 2312 56-57 0.5967667788298137 2312 58-59 0.4586854401893632 2312 60-61 0.28367441268673943 2312 62-63 0.5251257746399816 2312 64-65 0.5915346593528596 2312 66-67 0.7032782864089029 2312 68-69 0.618781360846647 2312 70-71 0.7130439483458133 2312 72-73 0.6578521807007327 2312 74-75 0.7394769422450906 2312 76-77 0.9413611331206546 2312 78-79 0.8754878947840439 2312 80-81 1.102005116681351 2312 82-83 1.0684634222577536 2312 84-85 1.4074572227766815 2312 86-87 1.8197428447958437 2312 88-89 2.004374102208974 2312 90-91 3.002261998570372 2312 92-93 4.00730217126279 2312 94-95 3.4426302758615073 2312 96-97 2.5245452403777477 2312 98-99 2.3143932696394565 2312 100-101 2.1025619332433596 2313 1 -7.180633263872103 2313 2 -2.772328756978027 2313 3 -1.6381827203028614 2313 4 -0.8361778486510261 2313 5 -0.6347524139878402 2313 6 -0.1538373760336782 2313 7 -0.10495218895737679 2313 8 0.03186649331681224 2313 9 -0.05491850733660897 2313 10-11 -0.33567361770957405 2313 12-13 -0.4230363811790454 2313 14-15 -0.6101232122699614 2313 16-17 -0.5899679987492874 2313 18-19 -0.652454732272993 2313 20-21 -0.5041597511544182 2313 22-23 -1.2340223436771254 2313 24-25 -0.4589620533441163 2313 26-27 -0.5093467065193309 2313 28-29 -0.7688703420003549 2313 30-31 -0.3393215825664484 2313 32-33 -0.5918023640259307 2313 34-35 -0.41754496353623693 2313 36-37 -0.11708210425754828 2313 38-39 -0.2380835844631335 2313 40-41 -0.28722603805621105 2313 42-43 -0.4597175533307407 2313 44-45 -0.35932392365841537 2313 46-47 -1.3805128499288877 2313 48-49 -0.8774194801034092 2313 50-51 -0.7684999701484472 2313 52-53 -1.1650860079941978 2313 54-55 -0.9822012209994462 2313 56-57 -0.8773385296945726 2313 58-59 -0.7046317391087626 2313 60-61 -0.41101198912840076 2313 62-63 -0.5763218483344872 2313 64-65 -0.7139180310091433 2313 66-67 -0.5056024429239869 2313 68-69 -0.6298861463022369 2313 70-71 -0.553812412445879 2313 72-73 -0.5218342816209578 2313 74-75 -0.29974967609020453 2313 76-77 -0.09958000770638264 2313 78-79 -0.16300921861588336 2313 80-81 0.008921613698813502 2313 82-83 0.2535464860939207 2313 84-85 0.3686733957851871 2313 86-87 0.8048387680974436 2313 88-89 1.341835051788646 2313 90-91 2.0937819538509217 2313 92-93 2.9375319897387477 2313 94-95 2.5142046462179017 2313 96-97 1.563709894168717 2313 98-99 1.2628710632234608 2313 100-101 1.1630010562861361 2314 1 0.5840487631534081 2314 2 -0.08773145804895321 2314 3 -1.1846965868731836 2314 4 -0.2277487520118484 2314 5 -0.08225532996680585 2314 6 -3.3793264686288325 2314 7 -2.6478275740252606 2314 8 -2.6313141371550515 2314 9 -3.245150614566654 2314 10-11 -2.4162875406713766 2314 12-13 -1.1812858944947848 2314 14-15 -1.0322873133352246 2314 16-17 -1.930565939143932 2314 18-19 -3.125020268695728 2314 20-21 -2.828457237500153 2314 22-23 -3.935576076308074 2314 24-25 -2.949924759691342 2314 26-27 -1.6518400012262262 2314 28-29 -1.5900267387909253 2314 30-31 -1.713406003590407 2314 32-33 -1.983933340253138 2314 34-35 -1.3377816085517722 2314 36-37 -1.75217421248081 2314 38-39 -3.061796380918622 2314 40-41 -2.2694996464579873 2314 42-43 -1.2875257403267746 2314 44-45 -1.9266783064918371 2314 46-47 -1.418611394009794 2314 48-49 -2.1090767024180472 2314 50-51 -2.952289104990971 2314 52-53 -1.6516958411149645 2314 54-55 -2.070807352459763 2314 56-57 -1.1532753746487288 2314 58-59 -0.67638420226967 2314 60-61 -0.9996691333231169 2314 62-63 -0.6851079629670025 2314 64-65 -1.1639829673029922 2314 66-67 -0.7731685748510309 2314 68-69 -0.5776413512019651 2314 70-71 -0.6565295769290067 2314 72-73 -1.7817302896361475 2314 74-75 -1.6124684100864677 2314 76-77 -0.6384998781553826 2314 78-79 -1.4549853352647197 2314 80-81 -0.526622665141204 2314 82-83 0.0028587390965917336 2314 84-85 -1.3010147378274795 2314 86-87 0.004673008726999939 2314 88-89 1.2652810167809605 2314 90-91 2.2009228544384136 2314 92-93 3.265357892454265 2314 94-95 3.0207810912549924 2314 96-97 2.214920318923369 2314 98-99 2.187225652526834 2314 100-101 2.163657747413774 2315 1 -3.0855293461933115 2315 2 -2.0807860661512407 2315 3 -2.968569683430548 2315 4 -4.325659041866857 2315 5 -4.126444550352449 2315 6 -3.2319781033809427 2315 7 -5.857551642427847 2315 8 -2.993477913962927 2315 9 -2.6261355415055547 2315 10-11 -3.8139568850394916 2315 12-13 -3.3343637352339925 2315 14-15 -2.377464714845921 2315 16-17 -2.7508761754073774 2315 18-19 -2.211986372463265 2315 20-21 -1.6100491553375846 2315 22-23 -1.0728819422312696 2315 24-25 -2.517089054079399 2315 26-27 -3.949250165692561 2315 28-29 -3.285192583176432 2315 30-31 -3.222874400375318 2315 32-33 -4.201290669672204 2315 34-35 -3.9427367638741977 2315 36-37 -3.1448837382517567 2315 38-39 -2.0826819324620267 2315 40-41 -1.7189799223217292 2315 42-43 -3.1195083120953626 2315 44-45 -6.847583897123016 2315 46-47 -3.562616783196166 2315 48-49 -2.6669984728801133 2315 50-51 -3.596509105877427 2315 52-53 -3.667200652156332 2315 54-55 -2.2884390643450345 2315 56-57 -1.7109257223081293 2315 58-59 -3.098494375961497 2315 60-61 -3.377826498777896 2315 62-63 -4.906299067286771 2315 64-65 -4.227056068249318 2315 66-67 -3.991343709836954 2315 68-69 -2.458058961171151 2315 70-71 -3.829790229588234 2315 72-73 -2.8982933897349987 2315 74-75 -1.9779301905074433 2315 76-77 -3.667126826714842 2315 78-79 -2.2326261744636007 2315 80-81 -1.8696047903615387 2315 82-83 -1.3940714834426764 2315 84-85 -2.6982973047017964 2315 86-87 -2.073814092120866 2315 88-89 -1.5109169503488715 2315 90-91 -0.6037545111953868 2315 92-93 0.050725402538414954 2315 94-95 -0.04678947109407616 2315 96-97 -0.38502116636284356 2315 98-99 -0.7193335104976413 2315 100-101 -1.1956503150128892 2316 1 -7.3986874510642195 2316 2 -6.14055346330618 2316 3 -3.3789633190144954 2316 4 -4.087437795600625 2316 5 -2.1531609020515 2316 6 -2.9545005060071503 2316 7 -3.374961662242711 2316 8 -2.6375253217646595 2316 9 -7.090320174634826 2316 10-11 -6.632703742756938 2316 12-13 -5.71874119360799 2316 14-15 -4.788709798097926 2316 16-17 -4.849455494012645 2316 18-19 -4.010231352204784 2316 20-21 -2.8454888530869695 2316 22-23 -3.6129603883910306 2316 24-25 -3.3079938835828457 2316 26-27 -4.09381904452615 2316 28-29 -4.206989894861287 2316 30-31 -6.919733670478536 2316 32-33 -4.942514755083142 2316 34-35 -5.590853982103873 2316 36-37 -4.191228561248828 2316 38-39 -6.359856983477908 2316 40-41 -3.89321978568789 2316 42-43 -4.574049686714595 2316 44-45 -3.0261873188838777 2316 46-47 -4.14972277793192 2316 48-49 -5.214859798405797 2316 50-51 -4.6816924673064655 2316 52-53 -6.337925151609202 2316 54-55 -5.023982198642496 2316 56-57 -4.154061720297072 2316 58-59 -3.022319310158405 2316 60-61 -3.124030177836243 2316 62-63 -4.1955734587929925 2316 64-65 -3.082437356511228 2316 66-67 -3.986089369790225 2316 68-69 -2.9672497309934087 2316 70-71 -2.579727531809276 2316 72-73 -2.1588852478960625 2316 74-75 -2.2461915101182406 2316 76-77 -2.9383844790970635 2316 78-79 -4.70532070023771 2316 80-81 -5.586102069514528 2316 82-83 -3.4358737488435587 2316 84-85 -2.130728839910148 2316 86-87 -2.2479766923042277 2316 88-89 -1.294908980041594 2316 90-91 -1.1910551056413077 2316 92-93 -1.0667470932472334 2316 94-95 -1.7637121783368528 2316 96-97 -3.152528264224614 2316 98-99 -4.753053529425273 2316 100-101 -3.9455438470863538 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 20495.0 3 7210.0 4 6934.0 5 7457.0 6 8353.0 7 9942.0 8 11762.0 9 14100.0 10 16634.0 11 19231.0 12 22066.0 13 24643.0 14 26761.0 15 29351.0 16 31935.0 17 34510.0 18 32904.0 19 32764.0 20 35050.0 21 38278.0 22 42078.0 23 46996.0 24 52739.0 25 58859.0 26 67020.0 27 75643.0 28 85851.0 29 96732.0 30 110738.0 31 125083.0 32 139892.0 33 155401.0 34 177055.0 35 206644.0 36 224922.0 37 182130.0 38 56613.0 39 1094.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.38418181303753 19.79635458240772 13.863527047407235 22.955936557147524 2 15.518043224526904 19.983103162863305 40.625663046548596 23.87319056606119 3 19.261711824476095 24.515658060334626 29.91590565384233 26.30672446134695 4 12.751926734983213 16.673133059501247 37.67967819227952 32.89526201323603 5 13.584911807079353 37.37918990851289 34.69291614674802 14.342982137659742 6 31.80129405550754 36.384250113182695 17.76166639228437 14.052789439025396 7 27.869893665153523 32.71652403630695 21.306080963725215 18.107501334814316 8 25.85864744963548 35.573462178690605 20.059877415100054 18.508012956573864 9 26.27108213891644 16.765320505975623 20.373036304012494 36.59056105109544 10-11 24.80580481145662 26.627162605089673 27.72557763272801 20.841454950725698 12-13 25.64540900987119 23.972640918052242 28.04301611925349 22.33893395282308 14-15 22.619881982739713 25.46720972129823 26.17017631837861 25.742731977583453 16-17 22.134927379513964 28.446696811936224 26.979816161306065 22.43855964724375 18-19 22.07871486632667 27.227183012975487 29.069609620491466 21.624492500206376 20-21 22.78692999492016 26.487858025235372 29.144899371356438 21.58031260848803 22-23 22.447283269107636 26.370988932730867 29.026275513760886 22.15545228440061 24-25 22.231381145343597 26.480189370867084 29.23173536824485 22.056694115544463 26-27 21.97426145172279 26.849908536421818 29.00791253828864 22.16791747356676 28-29 21.924036531263653 27.17120017439979 28.95570828888227 21.949055005454284 30-31 22.26629981900771 26.749055776348797 28.885155285206604 22.099489119436893 32-33 22.001068022384274 26.756771311262444 28.86889392315002 22.373266743203267 34-35 22.376708906597973 26.992867144478673 28.770964521933063 21.859459426990295 36-37 22.415052272224063 26.67136381502275 28.740969717982296 22.17261419477089 38-39 22.115492080939575 26.975024245961848 28.772282312450166 22.13720136064841 40-41 22.380867181881996 26.80608544473792 28.75757934607813 22.05546802730195 42-43 22.151096890334994 26.77453663065424 28.652897511736004 22.42146896727477 44-45 22.25200658339963 26.908734372952203 28.724767692350262 22.1144913512979 46-47 22.39704510919619 27.046703175881177 28.242724276482324 22.31352743844031 48-49 22.16692387907647 27.172833218837862 28.207134001334456 22.453108900751207 50-51 22.22103229270778 27.336761045723414 28.15962234355688 22.28258431801193 52-53 22.35097578883051 27.247937046795755 28.05554118813313 22.3455459762406 54-55 22.010744528154166 27.284628697401946 28.18715275154695 22.517474022896934 56-57 22.083596796262178 27.382886749811885 28.068838008866088 22.464678445059853 58-59 22.109856138370134 27.137728806162393 28.089868836145275 22.662546219322202 60-61 21.947428616534864 27.277239573368018 28.214518906461684 22.560812903635433 62-63 21.936402962688426 27.217388861113417 28.293275526034193 22.552932650163964 64-65 21.83431514804436 27.11483746703601 28.244390894164557 22.80645649075507 66-67 21.869639407839912 27.23923537859442 27.970866049681298 22.920259163884367 68-69 22.045385855707618 27.059245974366448 28.15233238887613 22.74303578104981 70-71 21.962298959065144 27.037255921959378 27.942917513554676 23.0575276054208 72-73 21.9005333894683 27.19704767845651 27.84940207463333 23.053016857441865 74-75 21.93193279114182 27.4207849616961 27.697452844359567 22.949829402802514 76-77 22.078361633284484 27.146812918135748 27.730818659682033 23.044006788897732 78-79 22.12757418470067 27.348859369651368 27.602707166135314 22.92085927951265 80-81 22.220161655733857 27.389929796929025 27.423972072455527 22.965936474881588 82-83 22.243154624942477 27.369737827200858 27.483004615669927 22.904102932186742 84-85 22.14917645402709 27.442097709658952 27.37018925806615 23.03853657824781 86-87 22.036789499333796 27.299976759512024 27.62740368985129 23.035830051302895 88-89 22.21635165921854 27.254675324006435 27.385410997591304 23.143562019183722 90-91 22.01472210099516 27.30676704983973 27.589083974881273 23.089426874283838 92-93 22.318831219159325 26.94740830685233 27.650252753593236 23.083507720395104 94-95 22.024440719067048 27.02002145731466 27.644238152566018 23.31129967105228 96-97 22.431823973170747 26.869418846210735 27.213500324910978 23.48525685570754 98-99 22.423396863184518 26.977978899926363 26.969561031798712 23.629063205090407 100-101 22.584443397723835 27.03443073612739 27.0350536136454 23.346072252503376 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 3033.0 1 1792.5 2 522.0 3 475.0 4 428.0 5 399.0 6 476.0 7 816.5 8 1238.5 9 1534.5 10 1577.0 11 1475.5 12 1488.5 13 1610.5 14 1799.0 15 1944.5 16 2130.0 17 2446.0 18 2824.0 19 3198.0 20 3715.5 21 4521.5 22 5510.5 23 6833.0 24 8623.5 25 10996.5 26 14010.0 27 17657.5 28 21169.0 29 24651.0 30 28740.0 31 33802.5 32 39414.0 33 43748.5 34 47290.5 35 51334.0 36 56395.5 37 61201.0 38 65013.5 39 68019.0 40 70624.5 41 73309.0 42 75535.5 43 77816.5 44 80792.0 45 83842.5 46 85934.5 47 87212.5 48 88395.5 49 87959.0 50 86252.5 51 84983.0 52 83296.0 53 81098.0 54 78002.0 55 72286.0 56 65233.0 57 58761.0 58 52700.5 59 46403.0 60 39115.5 61 32286.5 62 26319.5 63 20102.5 64 14576.0 65 10688.0 66 7833.5 67 5864.0 68 4414.0 69 3185.5 70 2393.5 71 1750.5 72 1221.0 73 917.0 74 705.0 75 519.5 76 367.0 77 247.5 78 162.5 79 116.5 80 86.0 81 61.5 82 44.5 83 26.0 84 16.0 85 17.0 86 12.0 87 7.0 88 5.0 89 4.0 90 5.0 91 4.0 92 2.5 93 4.0 94 3.0 95 4.0 96 3.0 97 1.0 98 2.5 99 1.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.13648019795622188 2 0.1236798280726239 3 0.12903115327479697 4 0.1411465535325168 5 0.1272759186084842 6 0.14375800023117724 7 0.1748813076070158 8 0.16148158930077444 9 0.17034338383557304 10-11 0.16582686536493896 12-13 0.15531686266787106 14-15 0.16068959317085282 16-17 0.1544606506355234 18-19 0.17006511492506005 20-21 0.17567330373693743 22-23 0.2096649214211407 24-25 0.2318836236605633 26-27 0.2443415087312222 28-29 0.2393968842444143 30-31 0.23049227910799833 32-33 0.23094179042498086 34-35 0.22160907927239099 36-37 0.23023541549829402 38-39 0.21702834489933084 40-41 0.22235826480069523 42-43 0.2143954928998617 44-45 0.20786687615321056 46-47 0.22370679875164284 48-49 0.26043829493935877 50-51 0.262578825020228 52-53 0.2628356886299323 54-55 0.26225774550809766 56-57 0.23672122164332776 58-59 0.237192138261119 60-61 0.2084020086734279 62-63 0.20020377846369877 64-65 0.20238711914618537 66-67 0.2103070804454015 68-69 0.20983616382761025 70-71 0.21544435263948764 72-73 0.2470813872347348 74-75 0.24915770141317795 76-77 0.2647407604019059 78-79 0.2623219614105237 80-81 0.2745657934730957 82-83 0.27458719877390436 84-85 0.24932894381964749 86-87 0.2519189852174993 88-89 0.2559217764687247 90-91 0.4330292353598445 92-93 0.5758025917538219 94-95 0.34216373342694584 96-97 0.28984917825050194 98-99 0.32063000081340143 100-101 0.34096503658165905 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2335870.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 65.07010314371452 #Duplication Level Percentage of deduplicated Percentage of total 1 79.74424679413066 51.889663640119075 2 12.097618746233499 15.743865992214962 3 3.8221359473793544 7.461203409758207 4 1.6193517528772134 4.214855423427007 5 0.8245075417428128 2.6825395391987663 6 0.47876742982455356 1.8692067624320878 7 0.2947392156454201 1.3425097813781501 8 0.2037913609177145 1.0608579899770907 9 0.14625862485027236 0.8565357424198564 >10 0.7145573405012863 8.115651682080975 >50 0.037045747035867604 1.616007015759725 >100 0.015310848716289224 1.8516658597729823 >500 0.0012681741103128781 0.5718651257240996 >1k 3.3373002902908016E-4 0.4723631128857487 >5k 6.674600580581602E-5 0.25120892285133967 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5784 0.24761651975495214 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4732 0.20257976685346357 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2815 0.12051184355293745 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 8.562120323476906E-5 2 0.0 0.0 0.0 0.0 8.562120323476906E-5 3 0.0 0.0 0.0 4.281060161738453E-5 8.562120323476906E-5 4 0.0 0.0 0.0 4.281060161738453E-5 1.284318048521536E-4 5 0.0 0.0 0.0 4.281060161738453E-5 1.284318048521536E-4 6 0.0 0.0 0.0 8.562120323476906E-5 1.284318048521536E-4 7 0.0 0.0 0.0 8.562120323476906E-5 1.284318048521536E-4 8 0.0 0.0 0.0 8.562120323476906E-5 1.284318048521536E-4 9 0.0 0.0 0.0 1.284318048521536E-4 1.284318048521536E-4 10-11 0.0 0.0 0.0 3.4248481293907624E-4 1.7124240646953812E-4 12-13 0.0 0.0 0.0 4.7091661779122984E-4 2.1405300808692264E-4 14-15 4.281060161738453E-5 0.0 0.0 4.7091661779122984E-4 2.568636097043072E-4 16-17 4.281060161738453E-5 0.0 0.0 5.137272194086144E-4 4.281060161738453E-4 18-19 4.281060161738453E-5 0.0 0.0 5.993484226433834E-4 4.281060161738453E-4 20-21 4.281060161738453E-5 0.0 0.0 7.919961299216137E-4 4.4951131698253754E-4 22-23 4.281060161738453E-5 0.0 0.0 0.0010274544388172288 4.7091661779122984E-4 24-25 4.281060161738453E-5 0.0 0.0 0.001369939251756305 4.7091661779122984E-4 26-27 4.281060161738453E-5 0.0 0.0 0.0021619353816779187 4.7091661779122984E-4 28-29 4.281060161738453E-5 0.0 0.0 0.006657048551503295 4.7091661779122984E-4 30-31 8.562120323476906E-5 0.0 0.0 0.022732429458831187 4.923219185999221E-4 32-33 8.562120323476906E-5 0.0 0.0 0.05867192951662549 5.137272194086144E-4 34-35 8.562120323476906E-5 0.0 0.0 0.11361933669253854 5.137272194086144E-4 36-37 8.562120323476906E-5 0.0 0.0 0.20739595953541934 5.137272194086144E-4 38-39 8.562120323476906E-5 0.0 0.0 0.3455457709547192 5.779431218346912E-4 40-41 8.562120323476906E-5 0.0 0.0 0.49463369108726085 6.42159024260768E-4 42-43 1.0702650404346132E-4 0.0 0.0 0.645284198178837 6.42159024260768E-4 44-45 1.284318048521536E-4 0.0 0.0 0.8208290701109222 6.42159024260768E-4 46-47 1.7124240646953812E-4 0.0 0.0 1.035096131205932 6.42159024260768E-4 48-49 1.7124240646953812E-4 0.0 0.0 1.2649890618912867 6.42159024260768E-4 50-51 1.7124240646953812E-4 0.0 0.0 1.504257514330849 7.27780227495537E-4 52-53 1.7124240646953812E-4 0.0 0.0 1.7345143351299517 8.134014307303061E-4 54-55 1.7124240646953812E-4 0.0 0.0 1.9864119150466422 8.134014307303061E-4 56-57 1.7124240646953812E-4 0.0 0.0 2.2778450855569874 8.348067315389983E-4 58-59 1.7124240646953812E-4 0.0 0.0 2.5937445148916676 8.990226339650751E-4 60-61 1.7124240646953812E-4 0.0 0.0 2.926040404645806 9.84643837199844E-4 62-63 1.7124240646953812E-4 0.0 0.0 3.2522571889702765 0.0011130756420519978 64-65 1.7124240646953812E-4 0.0 0.0 3.5840821621066237 0.0011130756420519978 66-67 1.7124240646953812E-4 0.0 0.0 3.954928998617218 0.00113448094286069 68-69 1.7124240646953812E-4 0.0 0.0 4.36655293316837 0.0011558862436693823 70-71 1.7124240646953812E-4 0.0 0.0 4.797955365666754 0.0011986968452867668 72-73 1.7124240646953812E-4 0.0 0.0 5.224520200182373 0.0013485339509476126 74-75 1.7124240646953812E-4 0.0 0.0 5.6415168652365075 0.001369939251756305 76-77 2.1405300808692264E-4 0.0 0.0 6.08505610329342 0.001369939251756305 78-79 2.1405300808692264E-4 0.0 0.0 6.557834982255006 0.001369939251756305 80-81 2.1405300808692264E-4 0.0 0.0 7.047759507164354 0.001369939251756305 82-83 2.568636097043072E-4 0.0 0.0 7.523470912336731 0.0014127498533736895 84-85 2.568636097043072E-4 0.0 0.0 7.966453612572618 0.0014127498533736895 86-87 2.568636097043072E-4 0.0 0.0 8.417334868806911 0.001455560454991074 88-89 2.568636097043072E-4 0.0 0.0 8.899788943734027 0.001541181658225843 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3575 0.0 53.35556 1 GTATCAA 6975 0.0 42.449257 1 ATCAACG 8445 0.0 34.835476 3 TCAACGC 8535 0.0 34.580074 4 TATCAAC 8665 0.0 34.44312 2 CAACGCA 8675 0.0 33.803215 5 AACGCAG 9065 0.0 32.4536 6 ACGCAGA 10020 0.0 28.934269 7 CGCAGAG 10305 0.0 28.132238 8 GCAGAGT 10935 0.0 25.166891 9 TACATGG 7645 0.0 23.646555 2 GTACATG 7770 0.0 23.633045 1 TGGTATC 1955 0.0 22.085897 2 ACATGGG 7805 0.0 22.007229 3 GAGTACT 6610 0.0 21.856617 12-13 CAGAGTA 10490 0.0 21.597542 10-11 GTGGTAT 2070 0.0 21.088573 1 AGAGTAC 9870 0.0 21.07943 10-11 GTACTTT 7005 0.0 20.693443 14-15 AGTACTT 7030 0.0 19.504715 12-13 >>END_MODULE