FastQCFastQC Report
Fri 27 May 2016
SRR939757_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939757_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2335870
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65690.28122284202459896No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59810.2560502082735769No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37670.16126753629268753No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA39600.056.354321
GTATCAA69700.037.365221
ATCAACG86850.029.7625123
TCAACGC87650.029.4896034
CAACGCA88650.029.156955
TATCAAC89050.029.0297132
AACGCAG93650.027.5789836
ACGCAGA107400.023.8287377
CGCAGAG107950.023.6200128
GTGGTAT20950.023.4212741
TGGTATC20000.021.9355772
GCAGAGT120650.021.0165029
GAGTACT71350.021.00599712-13
GTACATG75200.020.139491
TACATGG72550.019.9616782
CAGAGTA115850.019.54802110-11
GTACTTT77350.019.34631314-15
AGAGTAC107900.019.32846310-11
ACATGGG73800.018.3435923
AGTACTT76950.018.12980712-13
Produced by FastQC (version 0.11.5)