##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939757_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2335870 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.4820593611802 31.0 31.0 34.0 30.0 34.0 2 31.703376472149564 31.0 31.0 34.0 30.0 34.0 3 31.763033473609404 31.0 31.0 34.0 30.0 34.0 4 35.55745225547655 37.0 35.0 37.0 33.0 37.0 5 35.2867522593295 37.0 35.0 37.0 33.0 37.0 6 35.306396331987656 37.0 35.0 37.0 32.0 37.0 7 35.233909849435115 37.0 35.0 37.0 32.0 37.0 8 35.172366184762 37.0 35.0 37.0 32.0 37.0 9 36.79232363102399 39.0 37.0 39.0 32.0 39.0 10-11 36.65531172539568 39.0 35.0 39.0 32.0 39.0 12-13 36.59959993492788 39.0 35.0 39.0 32.0 39.0 14-15 37.67115806958435 39.0 36.5 41.0 32.0 41.0 16-17 37.588069755594276 39.0 36.0 41.0 32.0 41.0 18-19 37.56486683762367 39.0 36.0 41.0 32.0 41.0 20-21 37.489094641396996 39.0 36.0 41.0 32.0 41.0 22-23 37.35448719320853 39.0 36.0 41.0 31.5 41.0 24-25 37.193303993801024 39.0 36.0 40.5 31.0 41.0 26-27 36.903191316297566 39.0 36.0 40.0 30.5 41.0 28-29 36.6826257454396 39.0 35.5 40.0 30.0 41.0 30-31 36.44116646046227 38.0 35.0 40.0 30.0 41.0 32-33 36.403602298073096 38.0 35.0 40.0 30.0 41.0 34-35 36.488050062717534 38.0 35.0 40.0 30.0 41.0 36-37 36.4603706541888 38.0 35.0 40.0 30.0 41.0 38-39 36.33105716499634 38.0 35.0 40.0 30.0 41.0 40-41 36.16531870352374 38.0 35.0 40.0 30.0 41.0 42-43 35.9741105027249 38.0 35.0 40.0 29.0 41.0 44-45 35.740456018528434 38.0 35.0 40.0 28.5 41.0 46-47 35.4607251259702 38.0 34.0 40.0 27.5 41.0 48-49 35.1647908488058 38.0 34.0 40.0 26.5 41.0 50-51 34.90561889146228 38.0 34.0 40.0 26.0 41.0 52-53 34.62757751929688 38.0 33.0 40.0 25.5 41.0 54-55 34.32422416487219 38.0 33.0 40.0 24.0 41.0 56-57 32.92456236862497 36.5 31.5 39.5 16.5 41.0 58-59 29.41922474281531 34.5 23.5 39.0 8.0 40.0 60-61 29.412674292661833 34.0 24.0 38.0 9.0 40.0 62-63 31.444550638520123 35.0 30.0 39.0 12.5 40.0 64-65 31.03187527559325 35.0 30.0 38.5 6.5 40.0 66-67 25.49128932688891 28.5 18.0 36.0 2.0 39.0 68-69 17.738163296758806 13.5 7.0 29.5 2.0 35.5 70-71 16.57765800322792 15.0 7.5 26.0 2.0 33.5 72-73 18.7292826655593 19.5 7.5 30.0 2.0 35.0 74-75 19.438257694135377 19.0 8.5 31.0 2.0 35.5 76-77 20.397891363817337 23.5 9.0 31.5 2.0 34.5 78-79 23.488886581873132 29.0 12.0 34.0 2.0 36.0 80-81 26.20404324726975 31.5 22.5 35.0 2.0 36.0 82-83 27.275105849212498 33.0 26.0 35.0 2.0 36.0 84-85 27.29043696781071 33.0 26.0 35.0 2.0 36.0 86-87 27.00913792291523 33.0 26.0 35.0 2.0 36.0 88-89 26.568937483678457 33.0 25.0 35.0 2.0 35.0 90-91 24.864477047095946 32.0 12.5 34.5 2.0 35.0 92-93 22.65876525662815 31.0 2.0 34.0 2.0 35.0 94-95 18.196667408717094 23.0 2.0 33.0 2.0 35.0 96-97 16.8645076566761 15.0 2.0 32.5 2.0 35.0 98-99 15.76494411075959 4.5 2.0 32.0 2.0 34.0 100-101 15.134669095454798 2.0 2.0 31.5 2.0 34.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.34530128344203703 1101 2 0.32194350595178634 1101 3 0.2894685314678078 1101 4 0.2725760858819726 1101 5 0.38702234190221674 1101 6 0.2986525816653014 1101 7 0.29671471850876685 1101 8 0.3461799990551455 1101 9 0.41269604351020917 1101 10-11 0.43349105857501513 1101 12-13 0.348224983464128 1101 14-15 0.4061110645486039 1101 16-17 0.3574876555641282 1101 18-19 0.5179800038272901 1101 20-21 0.5597347219207123 1101 22-23 0.48287797054965864 1101 24-25 0.4474715406374372 1101 26-27 0.33037579139373463 1101 28-29 0.5362685866399346 1101 30-31 0.6491902427309526 1101 32-33 0.5647066378959522 1101 34-35 0.5239368049768416 1101 36-37 0.4924250356091662 1101 38-39 0.40948737213981445 1101 40-41 0.5135175245777361 1101 42-43 0.5463599023857739 1101 44-45 0.35127023060972107 1101 46-47 0.31168723075992233 1101 48-49 0.24262350663391885 1101 50-51 0.293989820034696 1101 52-53 0.3035610200583534 1101 54-55 0.3952849799922191 1101 56-57 0.9113601602794787 1101 58-59 -8.268408198440962 1101 60-61 -9.984577361208316 1101 62-63 -3.076122204947829 1101 64-65 -0.5052366443949268 1101 66-67 -1.3107742185559594 1101 68-69 -3.785146319050426 1101 70-71 -4.419550991011597 1101 72-73 -6.877650473981831 1101 74-75 -7.734226016771913 1101 76-77 -8.730496028608222 1101 78-79 -12.007645245239335 1101 80-81 -8.11504246708402 1101 82-83 -3.9572071695631124 1101 84-85 -3.0706480658958526 1101 86-87 -2.89419618186038 1101 88-89 -2.916631171210998 1101 90-91 -8.333199456822385 1101 92-93 -14.521361994415143 1101 94-95 -12.417656274192016 1101 96-97 -12.455555584913254 1101 98-99 -12.268238925758169 1101 100-101 -12.127056660513908 1102 1 0.48943136515387664 1102 2 0.49339723522628276 1102 3 0.43057937073775676 1102 4 0.32385445654431066 1102 5 0.4055341229061824 1102 6 0.4241017590548992 1102 7 0.39611258532806204 1102 8 0.4676770473717511 1102 9 0.5861566827662585 1102 10-11 0.6091230666977481 1102 12-13 0.5977863010491333 1102 14-15 0.7182943532989867 1102 16-17 0.7822319919642453 1102 18-19 0.7572208868917585 1102 20-21 0.7531674106895636 1102 22-23 0.7159992677796723 1102 24-25 0.7072971690795242 1102 26-27 0.721996483300714 1102 28-29 0.8341599017362427 1102 30-31 0.6838095151455832 1102 32-33 0.6174035980999122 1102 34-35 0.5128756283780902 1102 36-37 0.7757675322966691 1102 38-39 0.6604766081327469 1102 40-41 0.7237032021197578 1102 42-43 0.8286918721999683 1102 44-45 0.7417866555835175 1102 46-47 0.7332210829587851 1102 48-49 0.6304169559040034 1102 50-51 0.821314520856447 1102 52-53 0.864985435627446 1102 54-55 0.5619706053226778 1102 56-57 1.533528285108133 1102 58-59 -8.672835919137118 1102 60-61 -9.761730756960795 1102 62-63 -2.503510372153613 1102 64-65 0.26848764066031805 1102 66-67 -1.3896120163536843 1102 68-69 -3.759034822245864 1102 70-71 -4.15303731775243 1102 72-73 -6.758001744919493 1102 74-75 -7.669386032408298 1102 76-77 -8.797238951233703 1102 78-79 -11.957996416869712 1102 80-81 -7.985285911373687 1102 82-83 -3.5903505551411072 1102 84-85 -2.5802507795727827 1102 86-87 -2.3460830052580164 1102 88-89 -2.4454206545091672 1102 90-91 -8.291529066132469 1102 92-93 -14.426839964899212 1102 94-95 -12.24279600405814 1102 96-97 -12.46009678675411 1102 98-99 -12.16405790843627 1102 100-101 -11.886386504851242 1103 1 0.3371877425774059 1103 2 0.38158767913308367 1103 3 0.36542221440838674 1103 4 0.2196104564971293 1103 5 0.288814964218858 1103 6 0.24434375006389786 1103 7 0.3764263702728172 1103 8 0.35978437073811875 1103 9 0.4459107928821666 1103 10-11 0.4097527226096034 1103 12-13 0.343790730121583 1103 14-15 0.4695150799176844 1103 16-17 0.49199240360599106 1103 18-19 0.5263792327505357 1103 20-21 0.5791034356125664 1103 22-23 0.49938043683378197 1103 24-25 0.5330674297267777 1103 26-27 0.3842874697595704 1103 28-29 0.5069139044468329 1103 30-31 0.531696579385752 1103 32-33 0.37263246765584057 1103 34-35 0.3715376809619997 1103 36-37 0.22239174592522915 1103 38-39 0.1342442562853421 1103 40-41 0.294028540896619 1103 42-43 0.48421197779243386 1103 44-45 0.35733056418097675 1103 46-47 0.13241094886355853 1103 48-49 0.12701351111126513 1103 50-51 0.41464291230350625 1103 52-53 0.4114649314557113 1103 54-55 0.12882606942850572 1103 56-57 0.6101765687028688 1103 58-59 -6.980036545440491 1103 60-61 -8.940261054883276 1103 62-63 -2.7477326800517545 1103 64-65 -0.4758014075361068 1103 66-67 -1.8752526083618584 1103 68-69 -4.3092415309185625 1103 70-71 -5.054560610737374 1103 72-73 -7.674763244893729 1103 74-75 -8.280598340436814 1103 76-77 -9.101176753000736 1103 78-79 -12.276369989946613 1103 80-81 -8.457909351435621 1103 82-83 -4.448074518509063 1103 84-85 -3.4448750689438157 1103 86-87 -3.172842263999911 1103 88-89 -3.2413657623211343 1103 90-91 -8.993337448838103 1103 92-93 -14.843206921063562 1103 94-95 -12.830021228041685 1103 96-97 -12.896257984130937 1103 98-99 -12.413235450667358 1103 100-101 -11.994498337999254 1104 1 0.13364028416048868 1104 2 0.1756905855443165 1104 3 0.11410938953567751 1104 4 0.17946611465365692 1104 5 0.048478185548056274 1104 6 0.09727482926932396 1104 7 0.17291891396801873 1104 8 0.2387824262798972 1104 9 0.18144242895255047 1104 10-11 0.26254112761516524 1104 12-13 0.23798514358661293 1104 14-15 0.22795050308580045 1104 16-17 0.23977064185252317 1104 18-19 0.38741642884782124 1104 20-21 0.17379652429654158 1104 22-23 0.13602534057358895 1104 24-25 0.26480055412463344 1104 26-27 0.2961877958745589 1104 28-29 0.258727822443916 1104 30-31 0.22470783704682873 1104 32-33 0.18156042349635726 1104 34-35 0.2503538602340427 1104 36-37 0.21917895406759413 1104 38-39 0.15175095841699004 1104 40-41 0.1149795315533666 1104 42-43 0.17407862497362458 1104 44-45 0.06849593593482695 1104 46-47 0.1254488034845025 1104 48-49 0.08604752588502151 1104 50-51 -0.05382324493181301 1104 52-53 0.043422743272621744 1104 54-55 -0.07688541436006346 1104 56-57 0.5469212288209278 1104 58-59 -6.831661955706597 1104 60-61 -9.044885956855879 1104 62-63 -2.788720888673616 1104 64-65 -0.4926332295896465 1104 66-67 -1.9315948721222753 1104 68-69 -4.193114154948669 1104 70-71 -4.888283209068344 1104 72-73 -7.9652691613838975 1104 74-75 -9.029559130010949 1104 76-77 -9.404623797226895 1104 78-79 -12.391213120330564 1104 80-81 -8.547916592706848 1104 82-83 -4.825861932351184 1104 84-85 -3.883139098173448 1104 86-87 -3.6995957654736777 1104 88-89 -3.9279142787494656 1104 90-91 -9.603693497859714 1104 92-93 -15.53418722704594 1104 94-95 -13.074318215811529 1104 96-97 -12.775745221057047 1104 98-99 -12.125868722148537 1104 100-101 -11.678571523730128 1105 1 0.1523412288805659 1105 2 0.2205463154886793 1105 3 0.1762410282640765 1105 4 0.12397835088671627 1105 5 0.09120768852047689 1105 6 0.2279362052398639 1105 7 0.14844716702246785 1105 8 0.19994848899334272 1105 9 0.1461394380131935 1105 10-11 0.2309884299624514 1105 12-13 0.27227086814618673 1105 14-15 0.3219688041718385 1105 16-17 0.31038237709357475 1105 18-19 0.35107765289364323 1105 20-21 0.2608194559403003 1105 22-23 0.3263799184970395 1105 24-25 0.3794540084343936 1105 26-27 0.39676933495292843 1105 28-29 0.35050788578484315 1105 30-31 0.2959411152138074 1105 32-33 0.37180964297184715 1105 34-35 0.36948927370751505 1105 36-37 0.06523902456425645 1105 38-39 0.3185131548709492 1105 40-41 0.27588228804320636 1105 42-43 0.3551931231770453 1105 44-45 0.30916330798768854 1105 46-47 0.19463406444892684 1105 48-49 0.25073248884325494 1105 50-51 0.14536948641403313 1105 52-53 0.30102226070935956 1105 54-55 0.33554490478074683 1105 56-57 0.8740330499129385 1105 58-59 -6.946751137317772 1105 60-61 -9.083227262491569 1105 62-63 -2.6826011090633486 1105 64-65 -0.4083499771779522 1105 66-67 -1.7017654862847174 1105 68-69 -3.9503536377573525 1105 70-71 -4.590531784886423 1105 72-73 -6.002191633927325 1105 74-75 -6.645413418917835 1105 76-77 -8.706395203555413 1105 78-79 -12.065682574920803 1105 80-81 -8.306722248621618 1105 82-83 -4.255568160276024 1105 84-85 -3.2835195392585703 1105 86-87 -3.037148590779122 1105 88-89 -3.2582924814170475 1105 90-91 -8.673796060533157 1105 92-93 -11.804046440471412 1105 94-95 -11.950984645751307 1105 96-97 -12.158173913075066 1105 98-99 -11.632085320059527 1105 100-101 -11.411061569963788 1106 1 0.04540688609066734 1106 2 0.13098918147575844 1106 3 0.09415935491023575 1106 4 0.08038551727219101 1106 5 0.07551763327447247 1106 6 0.13454824026838708 1106 7 0.03823776149597791 1106 8 0.2792551019735612 1106 9 0.14513943328727663 1106 10-11 0.22146948138518496 1106 12-13 0.0870667792799864 1106 14-15 0.25430827647559084 1106 16-17 0.07892050818115592 1106 18-19 0.0848755441880229 1106 20-21 0.15163226696493126 1106 22-23 0.10381175891216543 1106 24-25 0.1758051254056454 1106 26-27 0.19472395666663544 1106 28-29 0.0405895969460488 1106 30-31 0.08773833542751674 1106 32-33 0.3139048324576166 1106 34-35 0.2709850274681642 1106 36-37 0.17192194839660857 1106 38-39 0.3668508124213119 1106 40-41 0.35448143076126826 1106 42-43 0.08732938090983566 1106 44-45 0.1609518325081254 1106 46-47 0.06628515920835554 1106 48-49 0.13050271460046758 1106 50-51 0.2440159828530568 1106 52-53 0.24487312956978968 1106 54-55 -0.1141850088766958 1106 56-57 0.7487857661355974 1106 58-59 -6.353573948389663 1106 60-61 -9.083990498612721 1106 62-63 -2.5273938538305742 1106 64-65 -0.3168920780385456 1106 66-67 -1.7525450268307488 1106 68-69 -3.7640677857448175 1106 70-71 -4.897209982798511 1106 72-73 -6.2573676980864175 1106 74-75 -6.659488154620746 1106 76-77 -8.545738962268828 1106 78-79 -11.83719047429651 1106 80-81 -7.871173876464852 1106 82-83 -3.9616797203495615 1106 84-85 -2.94430054483988 1106 86-87 -2.8939272144966246 1106 88-89 -3.0733010589623113 1106 90-91 -8.398372736226332 1106 92-93 -12.111892413278467 1106 94-95 -12.367033870936682 1106 96-97 -12.516666628068993 1106 98-99 -12.074726353334732 1106 100-101 -11.787600684042777 1107 1 0.06095636169485985 1107 2 0.10810323123002874 1107 3 0.09856731964651644 1107 4 0.05299533639363574 1107 5 -0.019810937430300157 1107 6 0.02912188367923818 1107 7 0.064503151372854 1107 8 0.09107047411724523 1107 9 -0.016865937336426384 1107 10-11 0.05912718012359619 1107 12-13 0.09301753167396498 1107 14-15 0.11121408789576748 1107 16-17 0.05293124942855343 1107 18-19 0.07081818998019429 1107 20-21 -0.020169425288202092 1107 22-23 0.047417676731491554 1107 24-25 0.101282654918748 1107 26-27 0.1712654776678022 1107 28-29 0.17533398565760194 1107 30-31 0.23347476953604485 1107 32-33 0.3643658954357818 1107 34-35 0.21501654188271857 1107 36-37 0.02995761744458747 1107 38-39 0.09724296928405352 1107 40-41 0.060419516589917066 1107 42-43 0.04624410959200986 1107 44-45 0.10698719755853148 1107 46-47 0.05023408761692849 1107 48-49 0.21616162932915017 1107 50-51 0.18541285167809463 1107 52-53 -0.016972642330699728 1107 54-55 -0.13244766277546205 1107 56-57 0.5309461755189915 1107 58-59 -7.130959062642081 1107 60-61 -9.142603763039936 1107 62-63 -2.865499948981814 1107 64-65 -0.4765338675708968 1107 66-67 -1.7368359525033448 1107 68-69 -4.455540653961872 1107 70-71 -4.946774252951016 1107 72-73 -6.358449422765954 1107 74-75 -7.083737027296431 1107 76-77 -9.446382423787803 1107 78-79 -12.729598135035221 1107 80-81 -8.795220084095192 1107 82-83 -4.617564606603803 1107 84-85 -3.617267561103752 1107 86-87 -3.434835153899776 1107 88-89 -3.467010842617377 1107 90-91 -8.83965053933724 1107 92-93 -12.262525741545174 1107 94-95 -12.171261970445219 1107 96-97 -12.646886519643195 1107 98-99 -11.931497899162366 1107 100-101 -11.482888721738476 1108 1 0.0383512124155736 1108 2 0.04981218591180436 1108 3 -7.116192836065238E-5 1108 4 -0.054197258274442106 1108 5 -0.18663949195848772 1108 6 -0.06603185790207533 1108 7 -0.04524125259962375 1108 8 -0.058348908840940794 1108 9 -0.12074501955677874 1108 10-11 0.08948271077332492 1108 12-13 0.03403898181561971 1108 14-15 0.113513725723557 1108 16-17 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1.7845361704712701 2312 86-87 1.493487837882327 2312 88-89 1.6498544030429336 2312 90-91 3.2826341199383897 2312 92-93 5.2652076277126625 2312 94-95 9.24993469666222 2312 96-97 9.991760276530645 2312 98-99 10.672038742340577 2312 100-101 9.975634714857923 2313 1 -0.35124415275104326 2313 2 -0.3050986282428809 2313 3 -0.37108503934024384 2313 4 -0.443387516845668 2313 5 -0.44282421569618435 2313 6 -0.36453530709102466 2313 7 -0.4691801305060963 2313 8 -0.3820484055774145 2313 9 -0.44618592680076574 2313 10-11 -0.5248827465086237 2313 12-13 -0.3786010439943297 2313 14-15 -0.52620003648709 2313 16-17 -0.5020289007601448 2313 18-19 -0.6582098656316688 2313 20-21 -0.5830132092626954 2313 22-23 -0.49710733551605557 2313 24-25 -0.5175153622353292 2313 26-27 -0.6075564013946462 2313 28-29 -0.7003194752003452 2313 30-31 -0.716310616897303 2313 32-33 -0.5951560863396068 2313 34-35 -0.6838847365293859 2313 36-37 -0.8508148445227519 2313 38-39 -1.0426564033739396 2313 40-41 -1.1815485898529303 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64-65 1.2250437700270531 2314 66-67 1.7212152988458165 2314 68-69 0.27798587136704356 2314 70-71 2.845888575104226 2314 72-73 8.607016254685245 2314 74-75 10.094764711043396 2314 76-77 9.125006433565478 2314 78-79 6.555894789182606 2314 80-81 3.5820911930711183 2314 82-83 2.1145151619927454 2314 84-85 1.6813427211868515 2314 86-87 1.5791445009421672 2314 88-89 1.6385605939476946 2314 90-91 2.817658784238489 2314 92-93 4.90993514863878 2314 94-95 8.922549109583102 2314 96-97 9.57720721971354 2314 98-99 10.075774212394125 2314 100-101 9.140170180634717 2315 1 -0.6892336367851506 2315 2 -0.554234450460676 2315 3 -0.6795174360675951 2315 4 -0.4092210443066904 2315 5 -0.7046951054157802 2315 6 -0.618649247877201 2315 7 -0.5872139878312055 2315 8 -0.6964978135717459 2315 9 -1.0961780087222124 2315 10-11 -0.8785689653660711 2315 12-13 -0.769103924295564 2315 14-15 -0.7864452798488983 2315 16-17 -1.013390141568948 2315 18-19 -0.9652077571063487 2315 20-21 -1.013804103661755 2315 22-23 -0.9244081565049882 2315 24-25 -1.1771873133999406 2315 26-27 -0.9292027381943058 2315 28-29 -0.9769933839587281 2315 30-31 -0.9340353413634688 2315 32-33 -0.791977520483556 2315 34-35 -0.825593211670629 2315 36-37 -0.9861967239089253 2315 38-39 -0.9174629130688885 2315 40-41 -0.8656390357259767 2315 42-43 -0.7522964040446709 2315 44-45 -0.6919124504599594 2315 46-47 -0.7407377806865583 2315 48-49 -0.8641660025595996 2315 50-51 -0.6750664213504649 2315 52-53 -0.4407221542953863 2315 54-55 -0.6247963122215623 2315 56-57 0.7611528044541984 2315 58-59 4.298611108639687 2315 60-61 3.969463301207867 2315 62-63 1.4228432344688002 2315 64-65 1.1460837005081679 2315 66-67 2.202369749537315 2315 68-69 6.296947208488167 2315 70-71 4.922987377191237 2315 72-73 9.180322933721861 2315 74-75 10.401116740932245 2315 76-77 9.147391884841259 2315 78-79 6.3737155874746065 2315 80-81 3.4440766681974573 2315 82-83 1.700591201090912 2315 84-85 1.2411537676040538 2315 86-87 1.1122114501195455 2315 88-89 1.4043229318104657 2315 90-91 2.632448742692034 2315 92-93 4.72128713304987 2315 94-95 8.73834867423825 2315 96-97 9.463712713210004 2315 98-99 9.614316322559157 2315 100-101 8.793188755916214 2316 1 -0.5869963316066702 2316 2 -0.5918606593152589 2316 3 -0.6625533985672263 2316 4 -0.44185094996427665 2316 5 -0.6910015555976585 2316 6 -0.5606796799595344 2316 7 -0.5827250338785817 2316 8 -0.6543807284269789 2316 9 -0.7497440927727226 2316 10-11 -0.7476821023252214 2316 12-13 -0.7110830447521934 2316 14-15 -0.8468631664605581 2316 16-17 -1.035080245966661 2316 18-19 -0.9876537837824841 2316 20-21 -0.894374520365389 2316 22-23 -1.033650533697191 2316 24-25 -2.5475504133910647 2316 26-27 -1.7140086560365546 2316 28-29 -1.2588217487888258 2316 30-31 -0.8609440007548272 2316 32-33 -0.9005748055514289 2316 34-35 -0.7910939066695946 2316 36-37 -0.8792612257277455 2316 38-39 -1.1197869452458562 2316 40-41 -0.7989671195990411 2316 42-43 -1.016039564959108 2316 44-45 -0.9297646079142012 2316 46-47 -0.8029460289934249 2316 48-49 -1.0599958608981126 2316 50-51 -0.9080399078793135 2316 52-53 -0.7590982966075188 2316 54-55 -0.8187476031451766 2316 56-57 0.27136159988791064 2316 58-59 3.7458147729108298 2316 60-61 3.7030892734670573 2316 62-63 1.1550261227070493 2316 64-65 1.0209083241736217 2316 66-67 6.074446761337509 2316 68-69 9.04204332536629 2316 70-71 5.874999251322489 2316 72-73 9.625138167507682 2316 74-75 10.249930732592262 2316 76-77 8.878958959634975 2316 78-79 6.291149562572944 2316 80-81 3.270118649092378 2316 82-83 1.7420348766003322 2316 84-85 1.4382212416524922 2316 86-87 1.1439301677725204 2316 88-89 1.2004068344514671 2316 90-91 2.7777048572042027 2316 92-93 4.816604318747679 2316 94-95 8.994620226599768 2316 96-97 9.568211870338917 2316 98-99 9.78230104736914 2316 100-101 8.776011265010348 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 23.0 4 36.0 5 85.0 6 224.0 7 549.0 8 1263.0 9 2230.0 10 3764.0 11 5651.0 12 7876.0 13 10910.0 14 14218.0 15 19722.0 16 26022.0 17 33969.0 18 37465.0 19 38589.0 20 40385.0 21 40587.0 22 39094.0 23 37672.0 24 39121.0 25 41428.0 26 54404.0 27 79704.0 28 112409.0 29 149728.0 30 178054.0 31 190497.0 32 194923.0 33 202004.0 34 207155.0 35 202003.0 36 172734.0 37 111026.0 38 38247.0 39 2097.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 41.521497012680214 19.886133723506884 14.8876559655832 23.704713298229706 2 15.604876705924461 20.805674562893316 39.559252096857676 24.030196634324547 3 18.995312468559632 24.65537075338293 30.43654536339393 25.91277141466351 4 13.213278136197648 17.305543544803434 37.18807125396533 32.29310706503358 5 13.375190421763858 37.61663065289175 34.86928798967738 14.138890935667003 6 31.930499467649486 36.846147490417906 17.312458286366475 13.910894755566133 7 27.743538809950895 32.65644064096033 21.284103995513448 18.315916553575327 8 25.878827861346558 35.93372396630458 19.777752757327367 18.40969541502149 9 25.853621990949836 16.52275169422956 20.25879008677709 37.364836228043515 10-11 24.73943327325579 26.731196513504607 27.709525786965884 20.819844426273722 12-13 25.445337283324843 23.869222174179214 28.167877493182413 22.517563049313534 14-15 22.256323295873287 25.52979804282479 26.162753736140314 26.051124925161606 16-17 21.96224533043363 28.530975610800258 27.014024753089856 22.492754305676257 18-19 21.873775199976198 27.306059638917983 29.085483362412802 21.734681798693018 20-21 22.492625873871404 26.507639551858624 29.219541327214273 21.780193247055703 22-23 22.24899074006687 26.38505139412724 29.086443166785823 22.279514699020066 24-25 22.107116406306858 26.640930359994346 29.14834729672456 22.103605936974233 26-27 21.914989166763345 26.86955207638106 28.94653248665271 22.268926270202886 28-29 21.907797650417262 27.178176048743502 28.875032697310882 22.038993603528358 30-31 22.141462776598367 26.810007130702502 28.85699612681476 22.19153396588437 32-33 21.879919224645644 26.81159110066909 28.817211298103786 22.491278376581484 34-35 22.290769908172358 27.01392452020009 28.71538696834784 21.979918603279714 36-37 22.34403482921327 26.709462373050606 28.762769876515932 22.183732921220187 38-39 22.01236666652393 27.030822460401403 28.746890134500347 22.209920738574322 40-41 22.316679577384875 26.83185534514439 28.77306640048748 22.07839867698325 42-43 22.005067126689088 26.832048066833142 28.64622330343002 22.51666150304775 44-45 22.169248468080248 26.97701097989122 28.70230622465045 22.151434327378087 46-47 22.294219540552287 27.037913322013797 28.26650451032997 22.401362627103946 48-49 22.091868021939245 27.17429007492369 28.263843924397992 22.469997978739077 50-51 22.123741089755573 27.33368489363374 28.212885391556174 22.329688625054516 52-53 22.31870169558803 27.116712472828354 28.17617398762388 22.38841184395974 54-55 21.97550940715707 27.224943256480707 28.203102621312954 22.59644471504927 56-57 22.06552328177661 27.223832588248737 28.170093241157655 22.540550888817 58-59 20.557991853831304 26.92766350561278 29.8381462204503 22.676198420105614 60-61 21.961291181392973 27.114079261936684 28.37550280777955 22.549126748890792 62-63 21.842990262907282 27.13112485479126 28.365787098250472 22.660097784050983 64-65 21.116357671213233 27.027322481677945 28.893041552284437 22.96327829482438 66-67 21.248734194522346 26.860815436988823 29.019944276345573 22.870506092143263 68-69 21.996124755269104 26.189074483047893 29.2629780468749 22.551822714808104 70-71 21.16526733659835 26.457125472983336 29.405986955819017 22.971620234599296 72-73 20.93535780751077 26.65919697388085 29.318188207925388 23.087257010682997 74-75 21.460435905800118 26.73953858026469 28.694571030121875 23.105454483813322 76-77 19.94637681002611 26.99568396075856 29.455534192357508 23.602405036857828 78-79 20.896165862618048 27.166498564017232 28.405851734537794 23.53148383882692 80-81 21.54922414213829 27.11043784189675 27.73806374167011 23.602274274294842 82-83 21.74626771424321 27.09214028621058 27.605718218178044 23.555873781368163 84-85 21.65457017183537 27.08090070956447 27.52816456570027 23.736364552899893 86-87 21.626564632221125 26.999812699007304 27.565290450325104 23.80833221844647 88-89 21.669199272133685 26.974720537564504 27.405267034509862 23.95081315579195 90-91 21.261603403586395 27.05390406144063 27.609026891859163 24.075465643113812 92-93 21.01522189620294 26.921990864074974 27.838349229900057 24.22443800982203 94-95 19.834488074419024 26.888567412978244 28.683928969333973 24.59301554326876 96-97 19.68138379776437 26.64124029717692 28.936981001080415 24.740394903978295 98-99 19.898777904640383 26.72969166335462 28.420573527037618 24.950956904967374 100-101 19.717452755689784 26.718882789845143 28.54857442462882 25.01509002983625 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 227.0 1 176.0 2 138.5 3 175.5 4 211.5 5 235.0 6 283.5 7 444.5 8 702.0 9 900.0 10 1075.5 11 1237.0 12 1400.5 13 1603.0 14 1805.5 15 2044.5 16 2267.5 17 2509.0 18 2898.0 19 3336.0 20 3782.5 21 4557.0 22 5574.5 23 6935.0 24 8668.0 25 10852.0 26 13782.5 27 17166.0 28 20734.5 29 24636.0 30 28927.0 31 33937.0 32 39411.5 33 44433.0 34 48976.5 35 53444.0 36 58247.0 37 62851.5 38 66868.0 39 70454.5 40 73549.5 41 76420.5 42 79206.0 43 81765.0 44 84860.5 45 87663.0 46 89412.0 47 90858.5 48 91631.0 49 91258.5 50 89820.0 51 87484.5 52 84811.0 53 81747.5 54 77405.0 55 71419.0 56 64278.5 57 56780.5 58 49104.0 59 41469.0 60 34054.5 61 27121.0 62 20934.0 63 15597.5 64 11347.0 65 8123.5 66 5817.5 67 4242.5 68 2977.0 69 2024.5 70 1441.5 71 1060.5 72 771.0 73 561.5 74 388.5 75 259.0 76 164.0 77 91.5 78 67.0 79 47.0 80 19.0 81 8.5 82 5.0 83 5.0 84 3.0 85 0.5 86 0.5 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.021362490207074883 2 0.004837597982764452 3 0.004238249560121069 4 0.0 5 0.06755512935223279 6 1.284318048521536E-4 7 0.0 8 1.284318048521536E-4 9 0.0 10-11 0.0 12-13 0.0 14-15 1.4983710566084585E-4 16-17 0.0 18-19 0.0012629127477128436 20-21 0.0 22-23 0.0 24-25 0.0 26-27 1.284318048521536E-4 28-29 0.002183340682486611 30-31 0.008369472616198676 32-33 3.852954145564608E-4 34-35 0.0011344809428606901 36-37 0.025215444352639484 38-39 0.023973936905735335 40-41 0.025729171572048102 42-43 0.026606788905204487 44-45 0.0276770539456391 46-47 0.028897156091734558 48-49 0.029710557522464865 50-51 0.024252205816248335 52-53 0.02078454708524019 54-55 0.015454627183875816 56-57 0.15345460149751483 58-59 0.7377979082740049 60-61 0.30277797993895206 62-63 3.638901137477685E-4 64-65 0.13829964852496074 66-67 1.3699820623579222 68-69 2.6299836891607837 70-71 2.1283932753106978 72-73 1.7856515987619175 74-75 1.505499021777753 76-77 1.083215247423872 78-79 0.40304040892686666 80-81 1.7124240646953812E-4 82-83 1.0702650404346132E-4 84-85 2.1405300808692265E-5 86-87 0.0024616095929996104 88-89 6.42159024260768E-5 90-91 0.2155299738427224 92-93 0.16557000175523467 94-95 1.2478434159435243 96-97 1.2168057297709205 98-99 0.9565386772380313 100-101 0.38540244106050425 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2335870.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.645102460002306 #Duplication Level Percentage of deduplicated Percentage of total 1 75.26740967352568 44.14064952202882 2 14.22168992008393 16.680649250354083 3 4.753756196626558 8.36353557663106 4 2.1233339230565913 4.9809254189780985 5 1.10455782507844 3.238845341236122 6 0.6357337885729561 2.2369603900887878 7 0.38960371690123885 1.5993844927529624 8 0.26275789216958767 1.2327570806767767 9 0.20530365975262332 1.0836048745445441 >10 0.9523152788050064 9.909084935595088 >50 0.05834185026067701 2.31190271799452 >100 0.022563309144837642 2.409177750639011 >500 0.0019015865714109563 0.7326352254892161 >1k 5.851035604327094E-4 0.5415950466737732 >5k 1.4627589010817734E-4 0.5382923763170866 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6569 0.28122284202459896 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5981 0.2560502082735769 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3767 0.16126753629268753 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 4.281060161738453E-5 4.281060161738453E-5 5 0.0 0.0 0.0 4.281060161738453E-5 4.281060161738453E-5 6 0.0 0.0 0.0 4.281060161738453E-5 8.562120323476906E-5 7 0.0 0.0 0.0 4.281060161738453E-5 8.562120323476906E-5 8 0.0 0.0 0.0 4.281060161738453E-5 8.562120323476906E-5 9 0.0 0.0 0.0 2.1405300808692264E-4 8.562120323476906E-5 10-11 0.0 0.0 0.0 4.281060161738453E-4 8.562120323476906E-5 12-13 0.0 0.0 0.0 5.565378210259989E-4 1.926477072782304E-4 14-15 0.0 0.0 0.0 5.565378210259989E-4 2.3545830889561492E-4 16-17 0.0 0.0 0.0 5.565378210259989E-4 2.7826891051299944E-4 18-19 0.0 0.0 0.0 6.207537234520757E-4 4.281060161738453E-4 20-21 0.0 0.0 0.0 8.990226339650751E-4 5.137272194086144E-4 22-23 0.0 0.0 0.0 0.0011130756420519978 6.421590242607679E-4 24-25 0.0 0.0 0.0 0.001455560454991074 6.849696258781525E-4 26-27 0.0 0.0 0.0 0.0022903671865300724 7.491855283042292E-4 28-29 0.0 0.0 0.0 0.007042343966059755 7.705908291129215E-4 30-31 0.0 0.0 0.0 0.023995342206544028 7.705908291129215E-4 32-33 0.0 0.0 0.0 0.06132618681690334 8.990226339650751E-4 34-35 0.0 0.0 0.0 0.11890644599228553 9.84643837199844E-4 36-37 4.281060161738453E-5 0.0 0.0 0.21585105335485277 0.0010274544388172288 38-39 4.281060161738453E-5 0.0 0.0 0.3592451634722823 0.0011130756420519978 40-41 4.281060161738453E-5 0.0 0.0 0.515354022270075 0.0011558862436693823 42-43 4.281060161738453E-5 0.0 0.0 0.6725545514091109 0.0011772915444780746 44-45 4.281060161738453E-5 0.0 0.0 0.8556768998274733 0.0012629127477128436 46-47 4.281060161738453E-5 0.0 0.0 1.0710142259629174 0.0013271286501389205 48-49 4.281060161738453E-5 4.281060161738453E-5 0.0 1.2808289844897192 0.0013271286501389205 50-51 4.281060161738453E-5 4.281060161738453E-5 0.0 1.4875613797000689 0.001369939251756305 52-53 4.281060161738453E-5 4.281060161738453E-5 0.0 1.6814291891243949 0.0014983710566084585 54-55 1.0702650404346132E-4 4.281060161738453E-5 0.0 1.8999130944787166 0.0014983710566084585 56-57 1.284318048521536E-4 4.281060161738453E-5 0.0 2.0971201308291985 0.0014983710566084585 58-59 1.284318048521536E-4 4.281060161738453E-5 0.0 2.2323588213385164 0.0014983710566084585 60-61 1.284318048521536E-4 6.42159024260768E-5 0.0 2.335832045447735 0.0014983710566084585 62-63 2.1405300808692264E-4 1.284318048521536E-4 0.0 2.42462123320219 0.0015839922598432277 64-65 2.568636097043072E-4 1.284318048521536E-4 0.0 2.522165188987401 0.0015839922598432277 66-67 2.568636097043072E-4 1.284318048521536E-4 0.0 2.603184252548301 0.0015839922598432277 68-69 2.568636097043072E-4 1.284318048521536E-4 0.0 2.695697962643469 0.0015839922598432277 70-71 2.996742113216917E-4 1.284318048521536E-4 0.0 2.855188858969035 0.0016482081622693043 72-73 2.996742113216917E-4 1.284318048521536E-4 0.0 3.055392637432734 0.0017338293655040733 74-75 2.996742113216917E-4 1.284318048521536E-4 0.0 3.2854354052237493 0.0017552346663127656 76-77 2.996742113216917E-4 1.284318048521536E-4 0.0 3.613214776507254 0.0017552346663127656 78-79 2.996742113216917E-4 1.284318048521536E-4 0.0 4.074220740024059 0.001776639967121458 80-81 2.996742113216917E-4 1.284318048521536E-4 0.0 4.590816269741039 0.0017980452679301501 82-83 2.996742113216917E-4 1.284318048521536E-4 0.0 5.088981835461734 0.0018408558695475349 84-85 2.996742113216917E-4 1.284318048521536E-4 0.0 5.492985482924992 0.0018836664711649193 86-87 3.2107951213038394E-4 1.284318048521536E-4 0.0 5.847500074918553 0.001969287674399688 88-89 3.4248481293907624E-4 1.284318048521536E-4 0.0 6.219502797672815 0.002012098276017073 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3960 0.0 56.35432 1 GTATCAA 6970 0.0 37.36522 1 ATCAACG 8685 0.0 29.762512 3 TCAACGC 8765 0.0 29.489603 4 CAACGCA 8865 0.0 29.15695 5 TATCAAC 8905 0.0 29.029713 2 AACGCAG 9365 0.0 27.578983 6 ACGCAGA 10740 0.0 23.828737 7 CGCAGAG 10795 0.0 23.620012 8 GTGGTAT 2095 0.0 23.421274 1 TGGTATC 2000 0.0 21.935577 2 GCAGAGT 12065 0.0 21.016502 9 GAGTACT 7135 0.0 21.005997 12-13 GTACATG 7520 0.0 20.13949 1 TACATGG 7255 0.0 19.961678 2 CAGAGTA 11585 0.0 19.548021 10-11 GTACTTT 7735 0.0 19.346313 14-15 AGAGTAC 10790 0.0 19.328463 10-11 ACATGGG 7380 0.0 18.343592 3 AGTACTT 7695 0.0 18.129807 12-13 >>END_MODULE