FastQCFastQC Report
Fri 27 May 2016
SRR939756_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939756_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2385247
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57640.2416521224007409No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48720.20425557604726052No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29610.12413808716665402No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN25510.10694909164543547No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA39200.054.0031741
GTATCAA68200.039.7311331
ATCAACG86100.030.9813353
TCAACGC86550.030.7658964
TATCAAC87150.030.7140962
CAACGCA87950.030.2258435
AACGCAG91000.029.3168036
ACGCAGA104350.025.2956227
GTACATG75050.024.6339871
CGCAGAG107450.024.30368
GTATAGG6600.024.2341521
TACATGG73450.024.2098922
ACATGGG77500.022.2177263
GTGGTAT20850.022.1112271
GCAGAGT111850.021.875179
TGGTATC20100.021.0639252
CAGAGTA106300.020.5159410-11
GAGTACT71250.020.40534612-13
AGAGTAC101600.019.68204110-11
CATGGGG49950.019.4022454
Produced by FastQC (version 0.11.5)