##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939756_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2385247 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.997945286169525 31.0 30.0 33.0 26.0 34.0 2 30.085308565528013 31.0 30.0 33.0 26.0 34.0 3 30.026559094299248 31.0 30.0 34.0 25.0 34.0 4 33.676469145543415 35.0 33.0 37.0 28.0 37.0 5 33.54433607924043 35.0 33.0 37.0 28.0 37.0 6 33.508717545813916 35.0 33.0 37.0 28.0 37.0 7 33.50370213231586 35.0 33.0 37.0 28.0 37.0 8 33.33429745430976 35.0 33.0 37.0 26.0 37.0 9 34.6342533917871 37.0 34.0 39.0 27.0 39.0 10-11 34.43932829597941 37.0 33.0 39.0 27.0 39.0 12-13 34.358432061752936 37.0 33.0 39.0 27.0 39.0 14-15 34.98324995273026 38.0 33.0 40.0 25.5 41.0 16-17 34.7665457707315 38.0 33.0 40.0 25.0 41.0 18-19 34.78879378110527 38.0 33.0 40.0 25.0 41.0 20-21 34.89884234211384 38.0 33.0 40.0 25.0 41.0 22-23 34.822970325505075 38.0 33.0 40.0 25.0 41.0 24-25 34.684732650329295 38.0 33.0 40.0 25.0 41.0 26-27 34.56393048602514 38.0 33.0 40.0 24.0 41.0 28-29 34.4365417921079 38.0 33.0 40.0 24.0 41.0 30-31 34.24029398213267 38.0 33.0 40.0 23.5 41.0 32-33 33.973184747743105 38.0 33.0 40.0 23.0 41.0 34-35 33.68296931093509 38.0 32.0 40.0 19.0 41.0 36-37 33.47837729174379 38.0 32.0 40.0 19.0 41.0 38-39 33.2084457081384 37.0 31.0 40.0 18.0 41.0 40-41 32.922842372299385 37.0 31.0 40.0 16.0 41.0 42-43 32.61925284886638 37.0 30.5 40.0 15.0 41.0 44-45 32.2291444030744 37.0 30.0 40.0 12.5 41.0 46-47 31.861522936618304 36.5 30.0 39.0 9.0 41.0 48-49 31.53481652005012 36.0 30.0 39.0 8.5 40.0 50-51 30.804095341069498 35.5 28.5 38.5 8.0 40.0 52-53 30.909113395803452 35.5 29.0 39.0 7.0 40.0 54-55 30.994184040478828 36.0 29.0 39.0 4.0 40.0 56-57 30.77847472400133 36.0 28.5 39.0 2.0 40.0 58-59 30.25127146161383 35.0 28.0 39.0 2.0 40.0 60-61 29.761567041065348 35.0 27.0 38.0 2.0 40.0 62-63 29.3342582550151 34.0 26.0 38.0 2.0 40.0 64-65 28.822879139980053 34.0 26.0 38.0 2.0 40.0 66-67 28.287187238889725 34.0 25.5 37.0 2.0 39.0 68-69 27.751613354927184 33.0 25.0 36.5 2.0 39.0 70-71 27.249142122388164 33.0 23.5 36.0 2.0 39.0 72-73 26.77642483147448 32.0 22.0 36.0 2.0 38.5 74-75 26.172585061421312 32.0 21.0 35.0 2.0 38.0 76-77 25.52389291339639 31.0 19.0 35.0 2.0 37.0 78-79 24.869913053029727 31.0 17.5 34.5 2.0 36.5 80-81 24.202978769075067 30.5 10.5 34.0 2.0 36.0 82-83 23.448259027262168 30.0 7.0 34.0 2.0 35.5 84-85 22.70756613466027 29.0 2.0 34.0 2.0 35.0 86-87 21.92492328886694 29.0 2.0 34.0 2.0 35.0 88-89 21.08026527231771 28.5 2.0 33.5 2.0 35.0 90-91 20.161707781206726 26.5 2.0 33.0 2.0 34.5 92-93 19.207152550658275 25.5 2.0 32.0 2.0 34.0 94-95 17.846351342229966 23.0 2.0 32.0 2.0 34.0 96-97 15.29499104285636 5.0 2.0 31.0 2.0 34.0 98-99 13.890970201408912 2.0 2.0 30.5 2.0 34.0 100-101 12.607585084479721 2.0 2.0 28.0 2.0 33.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.8450368231653762 1101 2 0.8375014222738244 1101 3 1.0290412543522613 1101 4 0.9047591893466773 1101 5 0.9439482246270359 1101 6 1.0613778842333446 1101 7 0.9268088485636454 1101 8 0.6488205645239802 1101 9 0.7557141190892267 1101 10-11 0.9570601323553021 1101 12-13 0.7625864415051282 1101 14-15 0.827680899796313 1101 16-17 1.0849916539032876 1101 18-19 0.9455715714201389 1101 20-21 0.864006135509797 1101 22-23 0.7843338720203477 1101 24-25 0.9674282452771621 1101 26-27 0.9642029487228072 1101 28-29 1.1893024136985986 1101 30-31 1.080032938870339 1101 32-33 1.0717206773767742 1101 34-35 0.6881612396806673 1101 36-37 0.818700534048233 1101 38-39 0.8307138532592973 1101 40-41 1.0207480185742313 1101 42-43 0.8476393663459447 1101 44-45 0.7574923000952367 1101 46-47 0.6574373185180526 1101 48-49 0.5824755096604974 1101 50-51 0.6140446212488158 1101 52-53 0.841170488454356 1101 54-55 0.8316708347475341 1101 56-57 0.6128172254011801 1101 58-59 0.3688192672285169 1101 60-61 0.6712428030794477 1101 62-63 0.541241567012964 1101 64-65 0.5753466843095687 1101 66-67 0.4899462147401259 1101 68-69 0.5110688819155698 1101 70-71 0.5906056928287917 1101 72-73 0.2404998514108989 1101 74-75 0.21052840068668388 1101 76-77 -0.21089971105502414 1101 78-79 -0.0880075221619272 1101 80-81 -0.26875397583701854 1101 82-83 -0.26261301377454416 1101 84-85 -0.27719766269978763 1101 86-87 -0.40044005822704776 1101 88-89 -0.4857512494268015 1101 90-91 -0.757444945805414 1101 92-93 -0.7398019015379695 1101 94-95 -0.39274296343721815 1101 96-97 1.271843588356269 1101 98-99 1.4748868219116105 1101 100-101 0.8989649699196871 1102 1 0.9110790297773228 1102 2 0.8558150416254655 1102 3 0.8142002466212013 1102 4 0.9714002109121154 1102 5 0.9370665438390091 1102 6 0.8554861705813224 1102 7 0.8885100790869771 1102 8 0.8820397642790851 1102 9 1.0664477529522358 1102 10-11 0.9443545852131976 1102 12-13 0.8636521481546069 1102 14-15 0.997271273574583 1102 16-17 1.1449318349569637 1102 18-19 1.0758046254168718 1102 20-21 0.8630549303740054 1102 22-23 0.8836476539994536 1102 24-25 1.1433015768738457 1102 26-27 1.339231187769002 1102 28-29 1.2700208297511821 1102 30-31 1.253827435144622 1102 32-33 1.4358470808665729 1102 34-35 1.2555701425412948 1102 36-37 1.074033666488802 1102 38-39 1.2326330229908677 1102 40-41 1.042589035135741 1102 42-43 0.8326984273452283 1102 44-45 1.1538751450310443 1102 46-47 1.0797124253297206 1102 48-49 0.9162377637508428 1102 50-51 0.9246966654246194 1102 52-53 0.9531692131900691 1102 54-55 0.8727240610263713 1102 56-57 0.9132987057379758 1102 58-59 0.793053354388011 1102 60-61 0.9028072131243796 1102 62-63 0.7705457495560459 1102 64-65 0.6057925153800987 1102 66-67 0.5894780904603572 1102 68-69 0.15442426194138648 1102 70-71 0.552953569914802 1102 72-73 0.2727166478116061 1102 74-75 0.2181074111463559 1102 76-77 0.23910319563507088 1102 78-79 0.23580860871713227 1102 80-81 0.06846006472713029 1102 82-83 0.022951995269306025 1102 84-85 -0.15943881642763458 1102 86-87 -0.29244106784193846 1102 88-89 -0.3632740908598677 1102 90-91 -0.36171086306114475 1102 92-93 -0.32743584844046936 1102 94-95 -0.38255933788954266 1102 96-97 0.9135542968969368 1102 98-99 0.6185205451093534 1102 100-101 0.21290120596941442 1103 1 0.8890110145904835 1103 2 0.8302933573512874 1103 3 0.9868771599480617 1103 4 1.0426125127186054 1103 5 1.1031340996994885 1103 6 1.0804972176709953 1103 7 0.955490328053493 1103 8 0.9825566517906381 1103 9 1.14142842054531 1103 10-11 0.9993996166392591 1103 12-13 0.9179278806057241 1103 14-15 1.0023329483133807 1103 16-17 1.1021636087125728 1103 18-19 0.9696809765050105 1103 20-21 1.020962538911668 1103 22-23 0.955201906838262 1103 24-25 1.132916585798725 1103 26-27 1.2920721067705685 1103 28-29 1.307921068717171 1103 30-31 1.2501411957483768 1103 32-33 1.3701764934521137 1103 34-35 1.4596295119268987 1103 36-37 1.3115544524601574 1103 38-39 1.3059584440884748 1103 40-41 1.4342090908363332 1103 42-43 1.2324242850936002 1103 44-45 1.342663003116641 1103 46-47 1.1374385563482043 1103 48-49 1.0363396150104975 1103 50-51 1.2423560923713772 1103 52-53 1.3594451956542706 1103 54-55 1.013206339168832 1103 56-57 1.0085587774859164 1103 58-59 0.7682355586893976 1103 60-61 1.0534459093352275 1103 62-63 0.7208869011279297 1103 64-65 0.8528864766920243 1103 66-67 0.9260923609745113 1103 68-69 0.8051101989962319 1103 70-71 0.7275317455170089 1103 72-73 0.6749201061321024 1103 74-75 0.5675163191706112 1103 76-77 0.39110024780640273 1103 78-79 0.34785662285740315 1103 80-81 0.1719353192462627 1103 82-83 0.15076972991821336 1103 84-85 0.050677555940684016 1103 86-87 -0.12399475379062253 1103 88-89 0.18153166932525622 1103 90-91 0.360603095227372 1103 92-93 0.33214008867252076 1103 94-95 0.671639355646743 1103 96-97 1.8774811884203295 1103 98-99 2.1004600070579436 1103 100-101 1.4498274840886172 1104 1 0.5773704212579602 1104 2 0.6487128285903054 1104 3 0.69468314486247 1104 4 0.7443192082601016 1104 5 0.7727151783673563 1104 6 0.884142193251165 1104 7 0.7595767881554849 1104 8 0.8609103042778372 1104 9 1.0281029578723633 1104 10-11 0.8751723917131713 1104 12-13 0.837067829073213 1104 14-15 0.8168520490738942 1104 16-17 1.0245920145211755 1104 18-19 0.8962393530891859 1104 20-21 0.6392637376519801 1104 22-23 0.7824166993334529 1104 24-25 0.9645331136186286 1104 26-27 1.1903364985990734 1104 28-29 1.181978573670584 1104 30-31 1.1561446766022598 1104 32-33 1.2357979590956845 1104 34-35 1.134301835453563 1104 36-37 0.9382109052206147 1104 38-39 0.827067852881882 1104 40-41 0.8950675550087581 1104 42-43 0.6645805862044654 1104 44-45 0.8136539976827706 1104 46-47 0.6382042449931937 1104 48-49 0.4004254515067416 1104 50-51 0.6251812815081657 1104 52-53 0.6773955452986691 1104 54-55 0.6070359729138382 1104 56-57 0.6905698068038859 1104 58-59 0.45233673021783005 1104 60-61 0.6245188938839767 1104 62-63 0.5509181947980473 1104 64-65 0.5378429801913036 1104 66-67 0.427895148492194 1104 68-69 0.326495269874691 1104 70-71 0.38953566006034634 1104 72-73 0.4562049182141763 1104 74-75 0.4155623781240294 1104 76-77 0.21698846080745327 1104 78-79 0.2925186994078466 1104 80-81 0.17969070705817103 1104 82-83 0.18008203450993676 1104 84-85 0.003123011697272915 1104 86-87 0.17373163635414102 1104 88-89 0.13945422599542923 1104 90-91 -0.14690320460794126 1104 92-93 0.04642625851437643 1104 94-95 0.3076977279790576 1104 96-97 1.7319840470857564 1104 98-99 1.9298382863029282 1104 100-101 1.684425130827302 1105 1 0.8475426631588157 1105 2 0.8844023236037302 1105 3 0.8502927457662146 1105 4 0.8938802670271926 1105 5 0.8383537357818867 1105 6 1.131858337781921 1105 7 0.9438507827588865 1105 8 0.8849414861185991 1105 9 1.1194656832550791 1105 10-11 1.0540497910033864 1105 12-13 0.8230469742606843 1105 14-15 1.0842207665148322 1105 16-17 1.1283777125227985 1105 18-19 1.212868930922987 1105 20-21 1.0687270724484463 1105 22-23 0.8294501031015429 1105 24-25 0.9267596685424238 1105 26-27 1.0906952224710054 1105 28-29 0.9043731682283962 1105 30-31 1.058563191755944 1105 32-33 1.1604740272710856 1105 34-35 1.2730825485799429 1105 36-37 1.1079374856642445 1105 38-39 1.0513423238556214 1105 40-41 1.0082754328129724 1105 42-43 1.0363474777859878 1105 44-45 1.0016934445487635 1105 46-47 0.9576404947337416 1105 48-49 0.9057995042953806 1105 50-51 0.9270788516792159 1105 52-53 0.9024572182432173 1105 54-55 0.9925186721642376 1105 56-57 0.8341816806746358 1105 58-59 0.8458661750082079 1105 60-61 1.0113108459475093 1105 62-63 0.9584295341132325 1105 64-65 1.1002545539487478 1105 66-67 0.9178422033326257 1105 68-69 0.5609036689641442 1105 70-71 0.7126534868589012 1105 72-73 0.6822509764198266 1105 74-75 0.7728746626940577 1105 76-77 0.679982227161414 1105 78-79 0.5481258333938044 1105 80-81 0.5638536903613911 1105 82-83 0.4323303323366652 1105 84-85 0.11123372696732403 1105 86-87 0.3706168549230533 1105 88-89 0.4929218617354536 1105 90-91 0.3784026931970139 1105 92-93 0.5689971100943971 1105 94-95 0.7497629294666943 1105 96-97 2.224529191681164 1105 98-99 2.1133548520811622 1105 100-101 1.4081049895494733 1106 1 0.9944264573294248 1106 2 1.1130446530731497 1106 3 1.121651715527907 1106 4 1.127743946501674 1106 5 1.0862355992950583 1106 6 1.048576064231412 1106 7 1.059050487573579 1106 8 1.0327420177639723 1106 9 1.3445003077642923 1106 10-11 1.258858765052544 1106 12-13 1.2147713917504106 1106 14-15 1.3831291525319855 1106 16-17 1.4328779892590262 1106 18-19 1.2455916256322652 1106 20-21 1.3491879357095726 1106 22-23 0.9708098264172875 1106 24-25 1.1446222774504733 1106 26-27 1.453652844150504 1106 28-29 1.4348689808065132 1106 30-31 1.3564703902758595 1106 32-33 1.3544401381266837 1106 34-35 1.5846927376700464 1106 36-37 1.6778964039763906 1106 38-39 1.4599580450718008 1106 40-41 1.5688920998694087 1106 42-43 1.3920967053930795 1106 44-45 1.6259903656308694 1106 46-47 1.5838098676988217 1106 48-49 1.4452493578720293 1106 50-51 1.7312080680089927 1106 52-53 1.8626791788869035 1106 54-55 1.929478679232055 1106 56-57 1.8196838205082813 1106 58-59 1.7663033402937884 1106 60-61 1.9767583141358358 1106 62-63 2.0178792317821532 1106 64-65 1.908312905016583 1106 66-67 1.7928130355940937 1106 68-69 1.774636022157523 1106 70-71 1.9565391753084747 1106 72-73 1.98299335707895 1106 74-75 1.8719623426050838 1106 76-77 1.697501579208872 1106 78-79 1.5919086227390729 1106 80-81 1.5765412018920664 1106 82-83 1.4240503324406042 1106 84-85 1.5620250886394906 1106 86-87 1.7320933531314147 1106 88-89 1.6653649099395267 1106 90-91 1.7476584676297016 1106 92-93 2.012000100924425 1106 94-95 2.2062160804919166 1106 96-97 3.4823922683124167 1106 98-99 3.821781405247002 1106 100-101 3.0057484441530384 1107 1 0.5647335772042439 1107 2 0.5938202765139664 1107 3 0.5306828438157218 1107 4 0.48003033627684033 1107 5 0.7818025185379724 1107 6 0.6778021080630694 1107 7 0.6743351303371483 1107 8 0.5433581940350578 1107 9 0.5036937621146791 1107 10-11 0.6241661904213842 1107 12-13 0.8024598271964223 1107 14-15 0.8028264234865432 1107 16-17 0.8546733310447081 1107 18-19 0.7212186391744524 1107 20-21 0.6791080205437083 1107 22-23 0.7297186017590533 1107 24-25 0.6887136087727228 1107 26-27 0.8339085064045904 1107 28-29 0.920069089748786 1107 30-31 0.8957302559206184 1107 32-33 1.021992219680122 1107 34-35 1.020286312561126 1107 36-37 0.8127122117906183 1107 38-39 0.998371588709297 1107 40-41 0.9071599383904427 1107 42-43 0.9166628262092402 1107 44-45 1.0766673196793874 1107 46-47 0.7910515776783207 1107 48-49 0.6888443390712098 1107 50-51 1.0313670802380734 1107 52-53 0.9323828186152134 1107 54-55 0.8778954952801215 1107 56-57 0.7573055650552121 1107 58-59 0.5153495775911949 1107 60-61 0.8386224593894447 1107 62-63 0.6446403530591382 1107 64-65 0.7182037142029429 1107 66-67 0.7441983215520338 1107 68-69 0.625508595939511 1107 70-71 0.5199514503690814 1107 72-73 0.6413096811263017 1107 74-75 0.48922933092071474 1107 76-77 0.4240867566387685 1107 78-79 0.4392276278848293 1107 80-81 0.39767652124723796 1107 82-83 0.6055482162472465 1107 84-85 0.5614104760835836 1107 86-87 0.487949518259736 1107 88-89 0.3474463391130662 1107 90-91 0.3715052276843416 1107 92-93 0.4473474683426062 1107 94-95 0.8277542895098939 1107 96-97 2.1115002568258383 1107 98-99 2.293573950985671 1107 100-101 1.770594677101034 1108 1 0.4908098990596379 1108 2 0.5090079768753419 1108 3 0.5341739237001057 1108 4 0.6132067804309003 1108 5 0.6746670173351887 1108 6 0.609884959740711 1108 7 0.595376117635233 1108 8 0.5399267359807425 1108 9 0.6634329634186784 1108 10-11 0.584788435375458 1108 12-13 0.4868585681410451 1108 14-15 0.4342139663674587 1108 16-17 0.45075489567920357 1108 18-19 0.4085403280194484 1108 20-21 0.44841243581122825 1108 22-23 0.48746525135711494 1108 24-25 0.7306048427869314 1108 26-27 0.591655156002659 1108 28-29 0.704683996783082 1108 30-31 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1.4264901621706905 2311 88-89 1.5623134370216754 2311 90-91 2.160795250722412 2311 92-93 1.9881274258601422 2311 94-95 2.136995027755024 2311 96-97 3.6368679675857116 2311 98-99 3.8537957325539267 2311 100-101 3.841776068241325 2312 1 0.185515784344215 2312 2 -0.12695922237061197 2312 3 -0.5918395748704839 2312 4 -0.19838512017756216 2312 5 -1.6987433901698843 2312 6 -0.40267370091800814 2312 7 0.03899195658934218 2312 8 0.39433990512340955 2312 9 0.4189713456958515 2312 10-11 0.3504557293755468 2312 12-13 0.21307433034492362 2312 14-15 0.37438624632886075 2312 16-17 -0.1876058422193978 2312 18-19 0.12009442976458473 2312 20-21 0.5183064588250872 2312 22-23 0.4885829420973167 2312 24-25 0.44607533973841385 2312 26-27 0.689887735975212 2312 28-29 0.6975229265908851 2312 30-31 0.4727328848178374 2312 32-33 -0.07897783961177396 2312 34-35 0.20727623155279673 2312 36-37 0.6359065927275225 2312 38-39 0.6944774638599824 2312 40-41 0.6521166000058969 2312 42-43 0.8264909905537294 2312 44-45 0.6122241936724535 2312 46-47 0.4807936824840304 2312 48-49 0.6971295536343121 2312 50-51 0.7018312878088437 2312 52-53 0.5065294977272607 2312 54-55 0.49385842493224175 2312 56-57 0.5481169268793948 2312 58-59 0.6529439099054706 2312 60-61 0.5904730480885867 2312 62-63 0.8002839479091683 2312 64-65 0.7204655798256105 2312 66-67 0.6412898127174529 2312 68-69 0.6335376284373169 2312 70-71 0.8042993717515792 2312 72-73 0.630829834955211 2312 74-75 0.8205568232399756 2312 76-77 0.789811769203812 2312 78-79 0.7635956911295239 2312 80-81 0.701981608106653 2312 82-83 1.0063671800813303 2312 84-85 1.02721024909399 2312 86-87 0.8838451257214253 2312 88-89 1.0314088379636352 2312 90-91 1.2432287110298326 2312 92-93 1.1670550019533152 2312 94-95 1.5391062254086378 2312 96-97 3.119435602581877 2312 98-99 3.591173271528479 2312 100-101 3.256168965806099 2313 1 -0.13947165176961107 2313 2 -0.5280209892797068 2313 3 -0.8777491765241763 2313 4 -0.04791421150041941 2313 5 -0.3519836875309963 2313 6 0.07692479994960877 2313 7 0.06979659052984744 2313 8 0.3482594820286167 2313 9 0.3044094460362601 2313 10-11 -0.6776858940681976 2313 12-13 -0.015756706720914337 2313 14-15 0.14964765813036962 2313 16-17 -0.33923476191483104 2313 18-19 -0.07403035333058483 2313 20-21 -2.1125484899237392 2313 22-23 -0.18187402777780193 2313 24-25 0.0545970918553067 2313 26-27 0.14477242083501807 2313 28-29 0.13805159358626895 2313 30-31 0.2554573635350792 2313 32-33 0.44779509066577106 2313 34-35 0.26330494121798154 2313 36-37 -0.43282171678973924 2313 38-39 0.2919098085181986 2313 40-41 0.337288725246502 2313 42-43 0.4324747783994738 2313 44-45 0.6318570437307685 2313 46-47 0.3658332350200375 2313 48-49 0.20187568016450186 2313 50-51 0.43717598869353225 2313 52-53 0.6014730669139716 2313 54-55 0.7085207171540127 2313 56-57 0.4350635825151272 2313 58-59 0.4936039050695591 2313 60-61 0.2694396803033392 2313 62-63 0.5318446670375678 2313 64-65 0.3472254128444483 2313 66-67 0.5610809558888619 2313 68-69 0.2867088836880711 2313 70-71 0.531492955161557 2313 72-73 0.4248123261130772 2313 74-75 0.4561057465386362 2313 76-77 0.5319249674477113 2313 78-79 0.34599137531609614 2313 80-81 0.6424068600955444 2313 82-83 0.7172229703734772 2313 84-85 0.7714714570286745 2313 86-87 0.8418719673974913 2313 88-89 1.12252308872932 2313 90-91 1.5021884805180754 2313 92-93 1.616837089644502 2313 94-95 1.8091826636180244 2313 96-97 3.3093834861596925 2313 98-99 3.499921825496294 2313 100-101 3.1201065232918026 2314 1 -0.3128034629933154 2314 2 -0.36914675496788263 2314 3 -1.3652345074883812 2314 4 -0.3359257941237601 2314 5 -0.6279422617007171 2314 6 -0.7030214747067944 2314 7 -0.49009226194933575 2314 8 -1.8836371237457783 2314 9 -1.6359628458112994 2314 10-11 -0.9303862831525365 2314 12-13 -0.8178060538305161 2314 14-15 -0.7540478298398341 2314 16-17 -0.7011951425053269 2314 18-19 -1.9558096807553014 2314 20-21 -1.4218468102008401 2314 22-23 -2.0377429138064898 2314 24-25 -1.9026496188500133 2314 26-27 -1.9776460802497766 2314 28-29 -2.959785329914709 2314 30-31 -1.6218635825467445 2314 32-33 -1.7336419543279504 2314 34-35 -1.978645203579596 2314 36-37 -3.176351740402154 2314 38-39 -3.6509136318912105 2314 40-41 -4.300840505409887 2314 42-43 -3.076863645128711 2314 44-45 -3.5607219620398425 2314 46-47 -3.168050980742553 2314 48-49 -1.8973191642609102 2314 50-51 -1.6332266585942357 2314 52-53 -2.8485502527124886 2314 54-55 -2.278091125963016 2314 56-57 -2.2282852252708203 2314 58-59 -4.021811077812622 2314 60-61 -2.8053768384505524 2314 62-63 -2.6331512279983826 2314 64-65 -2.001999745616626 2314 66-67 -2.6407372503956346 2314 68-69 -2.7413205827375826 2314 70-71 -2.44217952667368 2314 72-73 -1.7611672401566558 2314 74-75 -2.19506103330043 2314 76-77 -1.1483456526029663 2314 78-79 -0.8720205888204795 2314 80-81 -0.9746353358591158 2314 82-83 -0.831184699876065 2314 84-85 -0.8336137742097449 2314 86-87 -0.4553340101162924 2314 88-89 -0.3029407603783518 2314 90-91 0.05839136807045264 2314 92-93 0.2258220367295003 2314 94-95 0.6892083610478679 2314 96-97 2.3375597908166377 2314 98-99 2.4913869457232387 2314 100-101 2.2921455517994183 2315 1 -2.2359171188416127 2315 2 -1.5878503731320066 2315 3 -1.3282468815823627 2315 4 -1.570108251099505 2315 5 -1.4594408752839811 2315 6 -2.6221961788275507 2315 7 -2.0586808970700616 2315 8 -1.321729049232502 2315 9 -1.300885188910378 2315 10-11 -3.4749437699455115 2315 12-13 -1.8526412097740703 2315 14-15 -1.0005797614093481 2315 16-17 -2.0798593216131707 2315 18-19 -1.1433101780218706 2315 20-21 -0.8721932605131357 2315 22-23 -0.9076026598635281 2315 24-25 -3.077152956503248 2315 26-27 -4.241456092443883 2315 28-29 -5.8287825717928285 2315 30-31 -8.292585592593557 2315 32-33 -6.73321396730957 2315 34-35 -6.1226349132764994 2315 36-37 -3.8613272493666884 2315 38-39 -6.365671768185635 2315 40-41 -4.0080750922853206 2315 42-43 -3.951588196977063 2315 44-45 -2.9590456970649868 2315 46-47 -2.29015765864607 2315 48-49 -2.9372258387717523 2315 50-51 -2.887830071051372 2315 52-53 -2.2443685898620735 2315 54-55 -3.4840988717624484 2315 56-57 -3.3253743280848305 2315 58-59 -2.1048876214369017 2315 60-61 -2.219426807098362 2315 62-63 -2.9605528904374694 2315 64-65 -2.315213735973334 2315 66-67 -2.740093813721426 2315 68-69 -1.9859143380902324 2315 70-71 -2.4952509677799775 2315 72-73 -1.728296681235033 2315 74-75 -2.3384587518338904 2315 76-77 -1.293006315642078 2315 78-79 -1.3202963085359691 2315 80-81 -1.0703121265479432 2315 82-83 -0.8223079098477548 2315 84-85 -0.7586039076903539 2315 86-87 -1.237148374837794 2315 88-89 -1.7923434655385329 2315 90-91 -1.5380962110737464 2315 92-93 -0.6801054108390865 2315 94-95 -0.053621054975781846 2315 96-97 1.5037233112808295 2315 98-99 1.474215148791746 2315 100-101 1.231259782300226 2316 1 -3.5349582358026375 2316 2 -2.2289149739883136 2316 3 -2.3822623269336063 2316 4 -2.8374057160196173 2316 5 -2.464694864788882 2316 6 -5.863529380162138 2316 7 -3.6306555359369135 2316 8 -4.70852571968889 2316 9 -6.661050812392535 2316 10-11 -5.710448981924067 2316 12-13 -3.830696442862937 2316 14-15 -4.972464477377148 2316 16-17 -4.491615662308327 2316 18-19 -2.702761510547738 2316 20-21 -3.4838475767598744 2316 22-23 -4.125067876520951 2316 24-25 -2.8669821434333933 2316 26-27 -3.8338188465708036 2316 28-29 -3.634905191940021 2316 30-31 -3.2826865102294818 2316 32-33 -3.3084880007544797 2316 34-35 -5.5413222366489165 2316 36-37 -5.6418959802511495 2316 38-39 -5.651662607252852 2316 40-41 -5.051284363270312 2316 42-43 -4.20901376308769 2316 44-45 -3.523900715860581 2316 46-47 -3.9644926837500414 2316 48-49 -4.308677355217206 2316 50-51 -5.307084280121515 2316 52-53 -3.177344278478426 2316 54-55 -3.5686130671943808 2316 56-57 -2.875031867717027 2316 58-59 -3.32462719549531 2316 60-61 -3.3763469837510307 2316 62-63 -2.5944733835576343 2316 64-65 -2.8054196435363643 2316 66-67 -3.6382606236946025 2316 68-69 -2.8354566583915854 2316 70-71 -2.3383568513945256 2316 72-73 -2.396021459651621 2316 74-75 -3.6455815470641433 2316 76-77 -3.5190042478946673 2316 78-79 -3.4370340243775104 2316 80-81 -4.078060338236991 2316 82-83 -2.9634283006439297 2316 84-85 -2.5820458968615654 2316 86-87 -2.3325001217967944 2316 88-89 -2.3173909768504117 2316 90-91 -1.9591272361214074 2316 92-93 -1.6365454495340899 2316 94-95 -1.5432051855311322 2316 96-97 0.08531141797395847 2316 98-99 0.571380630440153 2316 100-101 0.3132032222345291 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 21007.0 3 6894.0 4 6723.0 5 7382.0 6 8197.0 7 9834.0 8 12212.0 9 14521.0 10 17458.0 11 20293.0 12 23278.0 13 25657.0 14 27734.0 15 30097.0 16 33023.0 17 35449.0 18 34069.0 19 34333.0 20 36517.0 21 40433.0 22 44404.0 23 50000.0 24 55863.0 25 62405.0 26 69751.0 27 77948.0 28 88465.0 29 99782.0 30 112698.0 31 127960.0 32 146878.0 33 168839.0 34 194398.0 35 216285.0 36 217912.0 37 164721.0 38 41418.0 39 409.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.508402091455864 19.063294412374113 14.057551975767591 23.37075152040244 2 15.600650611259773 19.99034576840338 40.53899994753135 23.8700036728055 3 19.288305946330343 24.49398448673875 29.88609587123246 26.33161369569844 4 12.855844878881953 16.68533660043179 37.62561268592609 32.83320583476017 5 13.728753429574848 37.23585188854905 34.71193670479521 14.323457977080889 6 31.844591715280735 36.357922711500066 17.764179599513824 14.033305973705367 7 27.977104957863265 32.61985068341247 21.272974083944288 18.130070274779975 8 25.93042169711739 35.53913191712342 20.05032620077912 18.480120184980066 9 26.262469750737257 16.77296686952516 20.341739510544567 36.62282386919301 10-11 24.850047972052344 26.625412801084984 27.68413605167965 20.840403175183017 12-13 25.72280517373462 23.891583265329515 28.020266992049276 22.365344568886588 14-15 22.747365455720217 25.43720147394256 26.074085051876413 25.74134801846081 16-17 22.228571296618377 28.453990354771676 26.892041080703898 22.425397267906053 18-19 22.145420187916645 27.196388641016217 28.981827725578707 21.676363445488427 20-21 22.81163927684658 26.440113096160722 29.132741808992495 21.615505818000198 22-23 22.441947839341424 26.36888065136647 29.003760452792044 22.18541105650006 24-25 22.20902715137961 26.53394274389817 29.18058399954784 22.07644610517438 26-27 22.086467843981268 26.751678678715905 28.947718432046738 22.21413504525609 28-29 22.01572367085687 27.113078391004862 28.930844172353552 21.94035376578471 30-31 22.291267439905866 26.723798117330645 28.85424020843839 22.130694234325095 32-33 21.96990040625038 26.823472505712175 28.81143922402689 22.395187864010556 34-35 22.481418371067946 26.93825757002875 28.71785920141427 21.86246485748903 36-37 22.46121882667444 26.636761053196228 28.750996072000305 22.151024048129024 38-39 22.13634241068587 26.906043425005922 28.753254102642796 22.204360061665415 40-41 22.45287508850328 26.77981644246536 28.74893171211918 22.018376756912183 42-43 22.189785038721933 26.754207154509864 28.611696247655686 22.444311559112514 44-45 22.27292834808516 26.925982079354455 28.686854174307243 22.114235398253136 46-47 22.439948315527403 26.99261500320402 28.228399146996104 22.339037534272478 48-49 22.22021891648758 27.204814826255607 28.145282813885263 22.429683443371555 50-51 22.257234672624364 27.32038108529022 28.185956674982503 22.236427567102915 52-53 22.328199983686744 27.259028419539916 28.062981777085263 22.34978981968808 54-55 22.04837324357855 27.203885095153552 28.178837473183084 22.56890418808482 56-57 22.107144433167115 27.371674851485984 28.04335537665929 22.477825338687612 58-59 22.163217432451855 27.084102032222734 28.077751283639596 22.674929251685814 60-61 22.04098772880607 27.241424512221407 28.204104234774025 22.5134835241985 62-63 21.985824988048158 27.179814534281416 28.271276171175597 22.56308430649483 64-65 21.834642527094008 27.113521801226582 28.191485339830297 22.860350331849112 66-67 21.88340355107133 27.253947385001826 27.963636592752522 22.899012471174323 68-69 22.102286833119024 27.05532061829643 28.031611301800723 22.810781246783822 70-71 21.958019367081004 27.020211885998798 27.905093657409097 23.1166750895111 72-73 21.927788362314324 27.14569054802871 27.825407608552904 23.101113481104065 74-75 21.94757816819699 27.396660320644017 27.646073931759275 23.009687579399714 76-77 22.089813132083847 27.163320566064623 27.66490985349801 23.08195644835352 78-79 22.079919497075295 27.31223129963128 27.59646311711763 23.011386086175794 80-81 22.229230605612603 27.37312712572255 27.36577077527947 23.03187149338538 82-83 22.201769997805787 27.3378657966041 27.484672040945174 22.975692164644936 84-85 22.18073584431457 27.394549463259477 27.34073251569118 23.08398217673477 86-87 22.24897981567868 27.258719920557894 27.361573579027855 23.130726684735574 88-89 22.31291004675082 27.237789135293706 27.181517530454013 23.267783287501462 90-91 22.27198570442761 27.368166463400794 27.173564958364814 23.18628287380678 92-93 22.43286175678149 27.16589434194298 27.15231885498543 23.248925046290097 94-95 22.294037725380832 27.092964240642996 27.202960699488372 23.4100373344878 96-97 22.5121639047652 26.81783912604529 27.156565988624955 23.513430980564557 98-99 22.46336338508695 26.86535087202798 26.914684759403716 23.75660098348135 100-101 22.543910843872673 26.902953959205476 27.010823787726313 23.54231140919554 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 3089.0 1 1777.0 2 441.0 3 397.5 4 356.5 5 368.5 6 496.5 7 837.0 8 1297.0 9 1606.5 10 1550.5 11 1415.0 12 1467.0 13 1554.5 14 1697.0 15 1932.5 16 2217.5 17 2502.5 18 2788.0 19 3189.5 20 3776.0 21 4517.0 22 5576.5 23 6899.0 24 8676.5 25 11142.5 26 14271.0 27 18054.5 28 21689.5 29 25297.0 30 29402.0 31 34571.5 32 40484.5 33 44871.5 34 48317.5 35 52624.0 36 57714.0 37 62662.0 38 66458.0 39 69141.0 40 71672.0 41 74449.0 42 77206.5 43 79717.5 44 82165.0 45 85420.5 46 87620.0 47 89009.5 48 90398.0 49 89873.0 50 88540.0 51 86770.5 52 84633.5 53 82575.5 54 79596.0 55 73916.5 56 66794.5 57 60049.0 58 54026.0 59 47591.5 60 39974.5 61 33112.0 62 26840.5 63 20540.5 64 15008.0 65 10976.5 66 8145.0 67 6146.0 68 4608.0 69 3401.0 70 2514.5 71 1743.0 72 1226.0 73 920.5 74 686.0 75 537.5 76 419.5 77 288.0 78 167.5 79 110.5 80 73.5 81 50.0 82 36.0 83 22.0 84 20.5 85 19.0 86 11.0 87 10.5 88 12.0 89 6.0 90 4.0 91 3.5 92 1.0 93 1.5 94 2.0 95 2.0 96 1.0 97 0.0 98 0.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content warn #Base N-Count 1 0.12506042351169502 2 0.12040681740716999 3 0.13231334113406285 4 0.14761573958587937 5 0.14220749465359353 6 0.14220749465359353 7 0.15076006803488276 8 0.15029889986236225 9 0.15864185134705128 10-11 0.1531078332768053 12-13 0.15262570291462477 14-15 0.139859729411671 16-17 0.14453429770585605 18-19 0.16233119672721524 20-21 0.193606783699969 22-23 0.21303873351480998 24-25 0.2335397550023121 26-27 0.23571982272695446 28-29 0.2368727431582557 30-31 0.2367469700202956 32-33 0.23813047453785707 34-35 0.20706450946170354 36-37 0.19478066632093027 38-39 0.2088882199621255 40-41 0.19643667930407208 42-43 0.1990779152012349 44-45 0.20392018101270015 46-47 0.2273139846732854 48-49 0.24511088369464462 50-51 0.26211121950892297 52-53 0.28563079630746835 54-55 0.27745554234005954 56-57 0.2449222239877044 58-59 0.23834009643445733 60-61 0.21465282211863174 62-63 0.20379440787473999 64-65 0.19482259070025032 66-67 0.19104939656144626 68-69 0.19729612908013303 70-71 0.20190781080533798 72-73 0.24012188255555922 74-75 0.2543342471450546 76-77 0.27284386061485455 78-79 0.26005692492224075 80-81 0.2662198086822874 82-83 0.2624885389228034 84-85 0.24672497229846638 86-87 0.23697755410655585 88-89 0.23966071438303876 90-91 0.2418617442973411 92-93 0.2498064141784897 94-95 0.25487926407621514 96-97 8.496499523948673 98-99 0.3174094758320627 100-101 0.3288757935760951 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2385247.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 64.44838217290851 #Duplication Level Percentage of deduplicated Percentage of total 1 79.7208167402263 51.37877664410507 2 11.86217203022938 15.289955928100179 3 3.948329715083422 7.6339038726704205 4 1.6715540008605214 4.309158042804525 5 0.8405209180784726 2.7085106676322668 6 0.49568005049187924 1.9167466397752329 7 0.2978269275096727 1.3436124551868556 8 0.20935774924871206 1.0794214587552757 9 0.1505698344202031 0.8733584009182322 >10 0.7480207611437302 8.47934355099142 >50 0.037775774422948126 1.6541269413537278 >100 0.015601351107543106 1.9026381986508805 >500 0.0012484739395796506 0.5553580605258399 >1k 4.5996408300197063E-4 0.6309924585801772 >5k 6.570915471456724E-5 0.24409667995007656 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5764 0.2416521224007409 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4872 0.20425557604726052 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2961 0.12413808716665402 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 2551 0.10694909164543547 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 8.384875864009053E-5 2 0.0 0.0 0.0 0.0 8.384875864009053E-5 3 0.0 0.0 0.0 0.0 8.384875864009053E-5 4 0.0 0.0 0.0 0.0 1.2577313796013578E-4 5 0.0 0.0 0.0 0.0 1.2577313796013578E-4 6 0.0 0.0 0.0 4.1924379320045264E-5 1.2577313796013578E-4 7 4.1924379320045264E-5 0.0 0.0 4.1924379320045264E-5 1.2577313796013578E-4 8 4.1924379320045264E-5 0.0 0.0 4.1924379320045264E-5 1.2577313796013578E-4 9 4.1924379320045264E-5 0.0 0.0 1.2577313796013578E-4 1.2577313796013578E-4 10-11 6.28865689800679E-5 0.0 0.0 2.7250846558029423E-4 1.6769751728018105E-4 12-13 8.384875864009053E-5 0.0 0.0 3.5635722422038473E-4 3.353950345603621E-4 14-15 8.384875864009053E-5 0.0 0.0 3.7731941388040736E-4 4.6116817252049786E-4 16-17 8.384875864009053E-5 0.0 0.0 3.7731941388040736E-4 5.869413104806336E-4 18-19 8.384875864009053E-5 0.0 0.0 4.821303621805205E-4 6.707900691207242E-4 20-21 8.384875864009053E-5 0.0 0.0 5.869413104806337E-4 8.175253967408826E-4 22-23 8.384875864009053E-5 0.0 0.0 7.546388277608147E-4 8.594497760609278E-4 24-25 1.2577313796013578E-4 0.0 0.0 0.0012158070002813125 8.804119657209505E-4 26-27 1.2577313796013578E-4 0.0 0.0 0.0020123702073621724 9.223363450409957E-4 28-29 1.2577313796013578E-4 0.0 0.0 0.00637250565664688 9.223363450409957E-4 30-31 1.2577313796013578E-4 0.0 0.0 0.021486244401523198 9.432985347010183E-4 32-33 1.2577313796013578E-4 0.0 0.0 0.05653502551308104 9.64260724361041E-4 34-35 1.2577313796013578E-4 0.0 0.0 0.11300716445718201 0.0010481094830011315 36-37 1.2577313796013578E-4 0.0 0.0 0.20706450946170354 0.0010481094830011315 38-39 2.5154627592027156E-4 0.0 0.0 0.3454149612178529 0.0010481094830011315 40-41 2.5154627592027156E-4 0.0 0.0 0.49474960035585414 0.0010481094830011315 42-43 2.5154627592027156E-4 0.0 0.0 0.6453838952527767 0.0010481094830011315 44-45 2.5154627592027156E-4 0.0 0.0 0.826224705449792 0.0010481094830011315 46-47 2.5154627592027156E-4 0.0 0.0 1.044042818207087 0.0010481094830011315 48-49 2.5154627592027156E-4 0.0 0.0 1.2798464896926816 0.001131958241641222 50-51 2.5154627592027156E-4 0.0 0.0 1.5180398507995188 0.0011738826209612672 52-53 2.725084655802942E-4 0.0 0.0 1.7470517728352661 0.0013625423279014712 54-55 2.934706552403168E-4 0.0 0.0 1.9998767423247992 0.0014463910865415617 56-57 2.934706552403168E-4 0.0 0.0 2.289679014374612 0.0015302398451816522 58-59 2.934706552403168E-4 0.0 0.0 2.601135228343228 0.0015512020348416747 60-61 2.934706552403168E-4 2.0962189660022632E-5 0.0 2.931813770230085 0.00159312641416172 62-63 3.353950345603621E-4 4.1924379320045264E-5 0.0 3.2469593295788655 0.0016350507934817652 64-65 3.353950345603621E-4 4.1924379320045264E-5 0.0 3.5684773945842925 0.0016350507934817652 66-67 3.353950345603621E-4 4.1924379320045264E-5 0.0 3.930242863736963 0.0016350507934817652 68-69 3.353950345603621E-4 4.1924379320045264E-5 0.0 4.332549207692118 0.0016350507934817652 70-71 3.353950345603621E-4 4.1924379320045264E-5 0.0 4.76135175937754 0.0016560129831417877 72-73 3.353950345603621E-4 4.1924379320045264E-5 0.0 5.182733695923315 0.001760823931441901 74-75 3.353950345603621E-4 4.1924379320045264E-5 0.0 5.598518727829864 0.0018027483107619462 76-77 3.353950345603621E-4 4.1924379320045264E-5 0.0 6.033966293637514 0.0018027483107619462 78-79 3.353950345603621E-4 4.1924379320045264E-5 0.0 6.525403868027085 0.0018027483107619462 80-81 4.192437932004526E-4 4.1924379320045264E-5 0.0 7.034680265817334 0.0018446726900819914 82-83 4.192437932004526E-4 4.1924379320045264E-5 0.0 7.550727450867772 0.0018865970694020367 84-85 4.192437932004526E-4 4.1924379320045264E-5 0.0 8.045644748740905 0.0018865970694020367 86-87 4.192437932004526E-4 4.1924379320045264E-5 0.0 8.491049354637067 0.0019075592590620592 88-89 4.192437932004526E-4 4.1924379320045264E-5 0.0 8.937355334688608 0.001928521448722082 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3920 0.0 54.003174 1 GTATCAA 6820 0.0 39.731133 1 ATCAACG 8610 0.0 30.981335 3 TCAACGC 8655 0.0 30.765896 4 TATCAAC 8715 0.0 30.714096 2 CAACGCA 8795 0.0 30.225843 5 AACGCAG 9100 0.0 29.316803 6 ACGCAGA 10435 0.0 25.295622 7 GTACATG 7505 0.0 24.633987 1 CGCAGAG 10745 0.0 24.3036 8 GTATAGG 660 0.0 24.234152 1 TACATGG 7345 0.0 24.209892 2 ACATGGG 7750 0.0 22.217726 3 GTGGTAT 2085 0.0 22.111227 1 GCAGAGT 11185 0.0 21.87517 9 TGGTATC 2010 0.0 21.063925 2 CAGAGTA 10630 0.0 20.51594 10-11 GAGTACT 7125 0.0 20.405346 12-13 AGAGTAC 10160 0.0 19.682041 10-11 CATGGGG 4995 0.0 19.402245 4 >>END_MODULE