FastQCFastQC Report
Fri 27 May 2016
SRR939756_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939756_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2385247
Sequences flagged as poor quality0
Sequence length101
%GC45

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67150.2815222071341039No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58420.2449222239877044No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37830.15859992696773123No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA39350.055.4895481
GTATCAA71100.039.5230261
ATCAACG87600.031.6265543
TCAACGC87650.031.608514
TATCAAC89050.031.3338662
CAACGCA89000.031.1290535
AACGCAG92800.030.0077366
ACGCAGA106650.025.843927
CGCAGAG108250.025.5057548
GTACATG74500.023.2559831
TACATGG74600.022.7727132
GCAGAGT118850.022.5922959
GAGTACT73150.021.95313612-13
ACATGGG75350.021.9098343
CAGAGTA113600.020.81778310-11
CATGGGG45250.020.75824
GTACTTT77950.020.44915414-15
TGGTATC19750.020.4231342
GTGGTAT20450.020.1940781
AGAGTAC106800.020.1443710-11
Produced by FastQC (version 0.11.5)